Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity

2018 ◽  
Vol 269 ◽  
pp. 150-156 ◽  
Author(s):  
Gong Zhang ◽  
Shasha Chen ◽  
Wu Zhou ◽  
Jing Meng ◽  
Kai Deng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Qualitative And Quantitative ◽  
Lycium Ruthenicum ◽  
Quantitative Analyses ◽  
Orbitrap Ms

scholarly journals Optimization of Ultrasound-Assisted Extraction via Sonotrode of Phenolic Compounds from Orange By-Products

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1120
Author(s):  
María del Carmen Razola-Diaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Phenolic Content ◽  
Orange Peel ◽  
Polar Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel is the main by-product from orange juice industry. It is a known source of bioactive compounds, mostly phenolic compounds, and it has been widely studied for its healthy activities. Thus, this research focuses on the establishment of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode. For this purpose, a Box–Behnken design of 27 experiments was carried out with four independent factors—ratio ethanol/water (v/v), time (min), amplitude (%), and pulse (%). Quantitative analyses of phenolic compounds were performed and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology (RSM). The extracts obtained in the established conditions were analyzed by High Performance Liquid Chromatography (HPLC) coupled to mass spectrometer detector and 74 polar compounds were identified. The highest phenolic content and antioxidant activity were obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W), and pulse 100%. The established method allows an increment of phenolics recovery up to 60% higher than a conventional extraction. Moreover, the effect of drying on phenolic content was also evaluated.

Assessment of the distribution of phenolic compounds and contribution to the antioxidant activity in Tunisian fig leaves, fruits, skins and pulps using mass spectrometry-based analysis

2015 ◽  
Vol 6 (12) ◽  
pp. 3663-3677 ◽  
Author(s):  
Sonda Ammar ◽  
María del Mar Contreras ◽  
Olfa Belguith-Hadrich ◽  
Antonio Segura-Carretero ◽  
Mohamed Bouaziz
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Composition ◽  
Qualitative And Quantitative

The qualitative and quantitative phenolic composition explains the differences in the antioxidant activity of fig leaves, fruits, pulps, and skins.

scholarly journals Optimization of Ultrasound Assisted Extraction of Phenolic Compounds from Orange By-Product

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 49
Author(s):  
María del Carmen Razola-Díaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Orange Peel ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel (OP) is the main by-product from orange juice industry. OP is a known source of bioactive compounds and is widely studied for its antioxidant, anti-inflammatory, anti-cancer, anti-rheumatic, anti-diabetic and cardioprotective activities. Thus, this research focuses on the establishments of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode; objective framed in the European SHEALTHY (non-thermal physical technologies to preserve healthiness of fresh and minimally processed fruit and vegetables) project. For this purpose, a Box Behnken design of 27 experiments was carried out with 4 independent factors (ratio ethanol/water, time (min), amplitude (%) and pulse (%)). Quantitative analyses of total phenolic compounds (TPC) were performed by Folin-Ciocalteu method and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology. The optimal extracts were characterized by HPLC coupled to mass spectrometer detectors. The highest phenolic content and antioxidant activity was obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W) and pulse 100%. The established method allows the extraction of 30.42 mg of gallic acid equivalents/g dry weight of total phenolic compounds from OP; this value suppose an increment up to 60% higher than conventional extraction.

scholarly journals Phytochemical, antioxidant and anticholinesterase profiles of Musanga cecropioïdes R. Br. (Urticaceae) from Côte d'Ivoire

2021 ◽  
Vol 16 (2) ◽  
pp. 094-102
Author(s):  
Djélé Alette Edwige Ziale ◽  
Kohué Christelle Chantal N’gaman-Kouassi ◽  
Fatou Diane Micheline Baguia-Broune ◽  
Janat Akhanovna Mamyrbekova-Békro ◽  
Yves-Alain Békro
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Quantitative Composition ◽  
Root Bark ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Tropical Plant ◽  
Anticholinesterase Properties

This study is devoted to Musanga cecropioides (Urticaceae), a tropical plant whose leaves and root bark are used in traditional medicine in the treatment of various pathologies. The objective of this work was to study qualitative and quantitative composition of phenolic compounds in extracts of the leaves (McF) and root bark (McR) Musanga cecropioides and to evaluate their antioxidant and anticholinesterase properties. Phytochemical screening using TLC showed that the extracts contain, along with phenolic compounds such as coumarins, flavonoids and tannins, other bioactive phytocompounds namely sterols, terpenes and alkaloids. Quantitative analysis of phenolic phytoconstituents by spectrophotometry showed that contents of total flavonoids and polyphenols in leaves (7.753% and 119.389 mg EAG / g, respectively) are higher than those in the root bark (1.41% and 105.944 mg EAG / g, respectively). The antioxidant activity of total and selective extracts evaluated by FRAP and DPPH methods was found to be significant compared to vitamin C. All the extracts of Musanga cecropioides showed good anticholinesterase activity with percentages of inhibition of the acetylcholinesterase (AChE) ranging from 51.952 to 63.589%.

scholarly journals Composition and Antioxidant Activity of Phenolic Compounds in Fruit of the Genus Rosa L.

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 545
Author(s):  
Mindaugas Liaudanskas ◽  
Irena Noreikienė ◽  
Kristina Zymonė ◽  
Rugilė Juodytė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Quantitative Composition ◽  
Rosa Multiflora ◽  
Epicatechin Gallate ◽  
Qualitative And Quantitative ◽  
Fruit Samples

We investigated the qualitative and quantitative composition of phenolic compounds in the fruit of Rosa L. cultivars grown in Lithuania. The highest total content of phenolic compounds (50.13 ± 4.17 mg GAE/g, p < 0.05) was determined in fruit samples of Rosa pimpinellifolia L. cultivar “Single Cherry”. The highest levels of hydroxycinnamic acid derivatives were determined in fruit samples of Rosa rugosa Thunb. cultivars “Dart’s Defender” and “Adam Chodun”. The highest flavonoid content was determined in fruit samples of Rosa multiflora Thunb. cultivar “Nana” and R. multiflora species. The strongest antioxidant activity evaluated by applying DPPH and FRAP assays was determined in fruit extracts of R. pimpinellifolia cultivar “Single Cherry” and R. rugosa cultivar “Adam Chodun”. Qualitative and quantitative analysis of phenolic compounds in Rosa L. fruit was performed by applying UHPLC. The following phenolic compounds were identified in fruit samples: caffeic acid, chlorogenic acid, quercetin, quercitrin, (+)-catechin, (−)-epicatechin, (−)-epicatechin gallate, rutin, phloridzin, and kaempferol-3-O-glycoside. A strong correlation was determined between the total amount of phenolic compounds determined in extracts of the fruit samples of Rosa L. cultivars and the radical scavenging and reducing activity of their extracts in vitro (R = 0.767 and 0.727, respectively, p < 0.05).

scholarly journals The Potential Antioxidant Activity and Characterization of Bioactive Compounds of Stahlianthus involucratus

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Wengkun Li ◽  
BingMin Wu ◽  
Yange Wang ◽  
Ying Lin ◽  
Lin An ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Comparative Assessment ◽  
H9c2 Cells ◽  
Stress Related Genes ◽  
Orbitrap Ms

Stahlianthus involucratus (S. involucratus) has anti-inflammatory, antinociceptive, and antipyretic activities; however, there are no literature reports on its antioxidant capacity. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of the aqueous and methanol extracts of S. involucratus (ASI and MSI). Moreover, the expression of oxidative stress-related genes in H2O2-induced H9c2 cells pretreated with the MSI was measured by RT-qPCR, and furthermore, MSI were characterized by UHPLC-Q-Orbitrap-MS/MS. The results indicated that the MSI had higher antioxidant contents and antioxidant capacity, and MSI could inhibit H2O2-induced oxidative stress in H9c2 cells by activating the Nrf2/HO-1 pathway. UHPLC-Q-Orbitrap-MS/MS characterized 15 phenolic compounds from the MSI. In conclusion, S. involucratus has the potential antioxidant capacity.

In situ microprobe quantitation of inorganic particle burden in paraffin sections of lung tissue

1988 ◽  
Vol 46 ◽  
pp. 990-991
Author(s):  
Jerrold L. Abraham
Keyword(s):  
Lung Tissue ◽  
Quantitative Information ◽  
Particulate Material ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Quantitative Analyses ◽  
Data Result

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).

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