Carbon dots and gold nanoclusters assisted construction of a ratiometric fluorescent biosensor for detection of Gram-negative bacteria

2021 ◽  
pp. 131750
Author(s):  
Li Fu ◽  
Qingmei Chen ◽  
Li Jia
Keyword(s):  
Carbon Dots ◽  
Gold Nanoclusters ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Fluorescent Biosensor

One-step synthesis of amikacin modified fluorescent carbon dots for the detection of Gram-negative bacteria like Escherichia coli

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 72471-72478 ◽  
Author(s):  
Soumen Chandra ◽  
Angshuman Ray Chowdhuri ◽  
Triveni Kumar Mahto ◽  
Arpita Samui ◽  
Sumanta kumar Sahu
Keyword(s):  
Escherichia Coli ◽  
Carbon Dots ◽  
Pathogenic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
One Step ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

In this paper, we report a one-step strategy to synthesize amikacin modified fluorescent carbon dots (CDs@amikacin) for assaying pathogenic bacteria, Escherichia coli.

scholarly journals Antibacterial Properties of Citric Acid/β-Alanine Carbon Dots against Gram-Negative Bacteria

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2012
Author(s):  
Anju Pandey ◽  
Asmita Devkota ◽  
Zeinab Yadegari ◽  
Korsi Dumenyo ◽  
Ali Taheri
Keyword(s):  
Citric Acid ◽  
Incubation Time ◽  
Carbon Dots ◽  
Bacterial Species ◽  
Synthesis Process ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli

While multi-drug resistance in bacteria is an emerging concern in public health, using carbon dots (CDs) as a new source of antimicrobial activity is gaining popularity due to their antimicrobial and non-toxic properties. Here we prepared carbon dots from citric acid and β-alanine and demonstrated their ability to inhibit the growth of diverse groups of Gram-negative bacteria, including E. coli, Salmonella, Pseudomonas, Agrobacterium, and Pectobacterium species. Carbon dots were prepared using a one-pot, three-minute synthesis process in a commercial microwave oven (700 W). The antibacterial activity of these CDs was studied using the well-diffusion method, and their minimal inhibitory concentration was determined by exposing bacterial cells for 20 h to different concentrations of CDs ranging from 0.5 to 10 mg/mL. Our finding indicates that these CDs can be an effective alternative to commercially available antibiotics. We also demonstrated the minimum incubation time required for complete inhibition of bacterial growth, which varied depending on bacterial species. With 15-min incubation time, A. tumefaciens and P. aeruginosa were the most sensitive strains, whereas E. coli and S. enterica were the most resistant bacterial strains requiring over 20 h incubation with CDs.

Selective inactivation of Gram-negative bacteria by carbon dots derived from natural biomass: Artemisia argyi leaves

2020 ◽  
Vol 8 (13) ◽  
pp. 2666-2672 ◽  
Author(s):  
Huibo Wang ◽  
Mengling Zhang ◽  
Yurong Ma ◽  
Bo Wang ◽  
Mingwang Shao ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Artemisia Argyi

Carbon dots derived from Artemisia argyi leaves exhibit specific antibacterial activities on Gram-negative bacteria.

scholarly journals A Novel Fluorescent Protein-Based Biosensor for Gram-Negative Bacteria

2002 ◽  
Vol 68 (12) ◽  
pp. 6343-6352 ◽  
Author(s):  
Yan Y. Goh ◽  
Bow Ho ◽  
Jeak L. Ding
Keyword(s):  
Lipid A ◽  
Fluorescent Protein ◽  
Computational Design ◽  
Site Directed Mutagenesis ◽  
Dual Function ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Fluorescent Biosensor ◽  
Wide Range

ABSTRACT Site-directed mutagenesis of enhanced green fluorescent protein (EGFP) based on rational computational design was performed to create a fluorescence-based biosensor for endotoxin and gram-negative bacteria. EGFP mutants (EGFPi) bearing one (G10) or two (G12) strands of endotoxin binding motifs were constructed and expressed in an Escherichia coli host. The EGFPi proteins were purified and tested for their efficacy as a novel fluorescent biosensor. After efficient removal of lipopolysaccharide from the E. coli lysates, the binding affinities of the EGFPi G10 and G12 to lipid A were established. The KD values of 7.16 × 10−7 M for G10 and 8.15 × 10−8 M for G12 were achieved. With high affinity being maintained over a wide range of pH and ionic strength, the binding of lipid A/lipopolysaccharide to the EGFPi biosensors could be measured as a concentration-dependent fluorescence quenching of the EGFP mutants. The EGFPi specifically tagged gram-negative bacteria like E. coli and Pseudomonas aeruginosa, as well as other gram-negative bacteria in contaminated water sampled from the environment. This dual function of the EGFPi in detecting both free endotoxin and live gram-negative bacteria forms the basis of the development of a novel fluorescent biosensor.

scholarly journals Amine-Coated Carbon Dots (NH2-FCDs) as Novel Antimicrobial Agent for Gram-Negative Bacteria

2021 ◽  
Vol 3 ◽  
Author(s):  
Asmita Devkota ◽  
Anju Pandey ◽  
Zeinab Yadegari ◽  
Korsi Dumenyo ◽  
Ali Taheri
Keyword(s):  
Pseudomonas Syringae ◽  
Carbon Dots ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Bacterial Diseases ◽  
Emergence Of Resistance ◽  
Coated Carbon

Multidrug resistance (MDR) is a major concern in battling infectious bacterial diseases. The overuse of antibiotics contributes to the emergence of resistance by eradicating the drug-sensitive strains, leaving behind the resistant strains that multiply without any competition. Nanoparticles are becoming popular as novel antimicrobial agents that follow a different mode of action from standard antibiotics and are therefore desirable against MDR bacteria. In this study, we synthesized carbon dots from different precursors including glucosamine HCL (GlcNH2·HCl) and 4,7,10-trioxa-1,13-tridecanediamine (TTDDA, and studied their antimicrobial effects in a diverse list of Gram-negative bacteria including Escherichia coli, Pseudomonas syringae, Salmonella enterica subsp. enterica serovar Typhimurium, Pectobacterium carotovorum, Agrobacterium tumefaciens, and Agrobacterium rhizogenes. We demonstrated the antimicrobial properties of these carbon dots against these bacteria and provided the optimum concentration and incubation times for each bacterial species. Our findings indicated that not all carbon dots carry antimicrobial properties, and there is also a variation between different bacterial species in their resistance against these carbon dots.

Bacterial-Mucosal Cell Junctional Complexes

1974 ◽  
Vol 32 ◽  
pp. 144-145
Author(s):  
Roger C. Wagner
Keyword(s):  
Intestinal Wall ◽  
Rat Colon ◽  
Mucosal Cell ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Mucosal Cells ◽  
Mucosal Lining ◽  
Degenerative Alterations ◽  
Junctional Complexes ◽  
Intestinal Mucosal Cells

Bacteria exhibit the ability to adhere to the apical surfaces of intestinal mucosal cells. These attachments either precede invasion of the intestinal wall by the bacteria with accompanying inflammation and degeneration of the mucosa or represent permanent anchoring sites where the bacteria never totally penetrate the mucosal cells.Endemic gram negative bacteria were found attached to the surface of mucosal cells lining the walls of crypts in the rat colon. The bacteria did not intrude deeper than 0.5 urn into the mucosal cells and no degenerative alterations were detectable in the mucosal lining.

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