Inhibitory effects of Allium cepa L. ethanolic extract on biological activities and expression of ERG11 in Candida albicans

2022 ◽  
pp. 100535
Author(s):  
Fatemeh Heidary ◽  
Masoomeh Shams-Ghahfarokhi ◽  
Mehdi Razzaghi-Abyaneh
Keyword(s):  
Candida Albicans ◽  
Allium Cepa ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Inhibitory Effects ◽  
Allium Cepa L

scholarly journals Phytochemical Screening and Anti-Hyperuricemia Activity Test In Vivo of Ethanolic Extract of Shallot (Allium cepa L.) Skin

2020 ◽  
Vol 3 (3) ◽  
pp. 146-151
Author(s):  
Syahrina Syahrina ◽  
Vivi Asfianti ◽  
Kasta Gurning ◽  
Iksen Iksen
Keyword(s):  
Uric Acid ◽  
Allium Cepa ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Purine Metabolism ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Allium Cepa L ◽  
Potassium Oxonate

Uric acid is the final product of purine metabolism that will be excreted through urine, feces, and sweat. Excessive production of uric acid can cause hyperuricemia, known as gout. The skin of shallots (Allium cepa L.) is one of the household wastes that are very rarely used by the community. Ethanol extract of shallot skin (EESS) was tested for phytochemical screening and anti-hyperuricemia activity using potassium oxonate. Mice were divided into five groups (Allopurinol, Na-CMC, EESS 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW) and uric acid levels were observed at 2-hour intervals for six hours. Phytochemical screening shows that EESS has potential compounds in the treatment of gout. Tests to reduce uric acid levels showed that EESS has better potential than allopurinol at concentrations of 300 mg/kg BW and 400 mg/kg BW after six hours of induction in reducing uric acid levels.

scholarly journals UJI AKTIVITTAS ANTIFUNGI EKSTRAK ETANOL UMBI BAWANG MERAH (Allium cepa L.) TERHADAP Candida albicans DAN Pityrosporum ovale

2019 ◽  
Vol 4 (2) ◽  
pp. 91
Author(s):  
Helen Anjelina Simanjuntak ◽  
Megawati Butar - Butar
Keyword(s):  
Candida Albicans ◽  
Allium Cepa ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Concentration Variation ◽  
Zone Diameter ◽  
Pityrosporum Ovale ◽  
Allium Cepa L ◽  
Bioactive Substance

Candida albicans dan Pityrosporum fungal caused micosis infections such as candidiasis and dandruff. The altervative medicine as antifungal bioactive substance is obtained from Bulbus Allium cepa L. exstract. The ekstraction of Bulbus Allium cepa L. has held by maserasi technic using etanol 96% as a solvent. The phytochem skrining result of the Bulbus Allium cepa L. extract is composed by alkaloid, flavanoid, tannin, and saponin compound. The test of these extract as antifungal is held by disk diffusion method with concentration variation of the extract is 50 %, 75%, and 100% (w/v). The extraction variation of the extract is implemented to inhibitory test and the result of the test to Candida albicans is 13,5 mm ; 16 mm ; 19 mm respectively and 12 mm ; 15 mm ; 17 mm to Pityrosporum . Base on the data ( zone diameter test of those fungal ) is concluded the extract of Aulbus Allium Cepa L. has a strong category inhibitory test. Keywords : Allium cepa, Candida albicans and Pityrosporum ovale

scholarly journals Antifungal activity of onion (Allium cepa L.) essential oil on Candida albicans

2019 ◽  
Vol 3 (1) ◽  
pp. 59
Author(s):  
Miftahur Rahmi ◽  
Felicia Aileen Agustia
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Allium Cepa ◽  
Inhibitory Concentration ◽  
Fungal Growth ◽  
Diffusion Method ◽  
Minimum Concentration ◽  
Allium Cepa L

Background:Onions (Allium cepa L.) contain essential oil that have sulfur compounds called thiosulfinates  or isothiocyanates which are known to have antibacterial and antifungal effects. Minimum Inhibition Concentration (MIC) test is needed to determine the minimum concentration of onion essential oil which can inhibit or kill  fungus test. Objectives: To determine the antifungal activity of essential oil in onion (Allium cepa L.) and to find out the Minimum Inhibitory Concentration (MIC) on Candida albicans. Methods:This research was an experimental study with disc diffusion method (Kirby Bauer) using Sabourond Dextrose Agar (SDA) media. Results: The results were obtained at concentrations of 20% (C1); 10% (C2); 5% (C3); 2.5% (C4); 1.25% (C5); 0.625% (C6); and 0.312% (C7) provided inhibition with an average diameter of 13.14 mm; 9.33 mm; 8.49 mm; 6.90 mm; 0 mm; 0 mm; and 0 mm respectively. Conclusion: Essential oil of onion (Allium cepa L.) could inhibit fungal growth of Candida albicans and Minimum Inhibitory Concentration (MIC) of essential oil onions (Allium cepa L.) indicated on concentration of 2.5% with a flat diameter average of 6.90 mm.

scholarly journals Assessment of Preservative Ability of Aqueous and Ethanolic Extracts of Onion (Allium cepa L ) on Soybean Daddawa- A Condiment

2020 ◽  
Vol 8 (3) ◽  
pp. 651
Author(s):  
Adelodun Kolapo ◽  
Temitope Popoola ◽  
Flora Oluwafemi
Keyword(s):  
Allium Cepa ◽  
Ethanolic Extract ◽  
Titratable Acidity ◽  
Absorption Capacity ◽  
Water Absorption Capacity ◽  
Fat Absorption ◽  
Allium Cepa L ◽  
Ethanolic Extracts ◽  
Key Factor ◽  
Glycine Max L

Reports have indicated that lipid peroxidation is a key factor in soybean daddawa deterioration and Alliums are rich in antioxidants. The present study was carried out to investigate the preservative abilities of aqueous and ethanolic extracts of Onion (Allium cepa L) on stored soybean daddawa compared with Sodium Chloride treated samples. Soybean (Glycine max (L). Merr.) seeds fermented into soybean daddawa was preserved with both extracts (at 3, 5 and 7% concentration) and NaCl (at the concentrations of 0, 3 and 5%). The samples stored at 30±2°C for up to 14 days were analyzed for peroxide value (POV), free fatty acid (FFA), pH, titratable acidity (TTA), water absorption capacity (WAC) and fat absorption capacity (FAC). Type of solvent used in extraction did not have significant effect on all the storage indices. Extracts of Onion significantly lowered the POV, FFA and TTA generated in stored soybean daddawa. The pH of stored soybean daddawa samples treated with the NaCl and aqueous extract was not significantly affected while the ethanolic extract significantly reduced the pH from 8.26 – 8.34 to 5.72 – 5.89. The tested extracts lowered the WAC significantly while the FAC of treated soybean daddawa was not significantly affected compared to those treated with NaCl. Results of this study indicate that both extracts of Onion are more effective in lowering peroxidation in stored soybean daddawa and thus could be exploited for soybean daddawa preservation.

scholarly journals Antioxidant potential and α-glucosidase inhibitory activity of onion (Allium cepa L.) peel and bulb extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
S. Masood ◽  
A. ur Rehman ◽  
M. A. Ihsan ◽  
K. Shahzad ◽  
M. Sabir ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Onion Bulb ◽  
Onion Peel ◽  
Allium Cepa L ◽  
Food Applications

Abstract Allium cepa L. is a commonly consumed vegetable that belongs to the Amaryllidaceae family and contains nutrients and antioxidants in ample amounts. In spite of the valuable food applications of onion bulb, its peel and outer fleshy layers are generally regarded as waste and exploration of their nutritional and therapeutic potential is still in progress with a very slow progression rate. The present study was designed with the purpose of doing a comparative analysis of the antioxidant potential of two parts of Allium cepa, i.g., bulb (edible part) and outer fleshy layers and dry peels (inedible part). Moreover, the inhibitory effect of the onion bulb and peel extracts on rat intestinal α-glucosidase and pancreatic α-amylase of porcine was also evaluated. The antioxidant potential of onion peel and bulb extracts were evaluated using 2,2-diphenyl- 1-picryl hydrazyl (DPPH), ferric-reducing antioxidant power assay (FRAP), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay, H2O2 radical scavenging activity and Fe2+ chelating activity. Total flavonoids and phenolic content of ethanolic extract of onion peel were significantly greater as compared to that of onion bulb. Ethanolic extract of onion peel also presented better antioxidant and free-radical scavenging activity as compared to the ethanolic extract of bulb, while the aqueous extract of bulb presented weakest antioxidative potential. Onion peel extract’s α-glucosidase inhibition potential was also correlated with their phenolic and flavonoid contents. The current findings presented onion peel as a possible source of antioxidative agents and phenolic compounds that might be beneficial against development of various common chronic diseases that might have an association with oxidative stress. Besides, outer dry layers and fleshy peels of onion exhibited higher phenolic content and antioxidant activities, compared to the inner bulb. The information obtained by the present study can be useful in promoting the use of vegetable parts other than the edible mesocarp for several future food applications, rather than these being wasted.

scholarly journals Cytological changes in meristematic cells of Allium cepa L. root tip treated with extracts from callus of Catharanthus roseus (L.) G. Don

2014 ◽  
Vol 68 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Agnieszka Pietrosiuk ◽  
Mirosława Furmanowa ◽  
Alicja Zobel ◽  
Mieczysław Kuraś ◽  
Agnieszka Michalak
Keyword(s):  
Allium Cepa ◽  
Catharanthus Roseus ◽  
Ethanolic Extract ◽  
Root Tip ◽  
Root Cells ◽  
Irregular Structures ◽  
Allium Cepa L ◽  
Green Callus ◽  
Pharmacologically Active ◽  
Tip Cells

The effect of an ethanolic extract from callus of <em>Catharanthus roseus</em> on <em>Allium cepa</em> root cells divisions was investigated. Two lines: white and green callus, were established on solid B5 medium with IAA 1 mg/l and kinetin 0.1 mg/l. The HPLC analysis of callus extracts showed the presence of indole alkaloids, however not known pharmacologically active alkaloids or derivatives used in semisynthesis (vinblastine, ajmalicine, serpentine, yohimbine, vindoline and catharanthine) have been found. The ethanolic extract of <em>C. roseus</em> callus inhibited the number of mitoses in <em>Allium cepa</em> root tip cells. Short (1-3 hours) treatment resulted in an increase in the index of late prophases, with characteristic light spaces, and the index of metaphases with twisted chromosomes forming an equatorial plate or irregular structures of c-metaphases. At the same time, the percentage of anaphases and telophases decreased significantly. Longer treatment of the root tip inhibited mitotic activity, stopping it completely already after 12 hours. Interphase nuclei became at first denser and homogeneous, eventually their structure became , partitioned into zones and formation of chromatin territories, with distinct large nucleoli has been observed. Electron microscope observations revealed well developed rough endoplasmic reticulum and thick, invaginating cell membrane.

The Cytotoxicity of Ethanolic Extract of Allium cepa L. on Hela Cell Lines

2021 ◽  
pp. 4969-4972
Author(s):  
Annise Proboningrat ◽  
Shara Jayanti ◽  
Amaq Fadholly ◽  
Arif N. M. Ansori ◽  
Naimah Putri ◽  
...  
Keyword(s):  
Side Effects ◽  
Allium Cepa ◽  
Hela Cells ◽  
Cytotoxic Effect ◽  
Test Procedure ◽  
Ethanolic Extract ◽  
Cytotoxicity Test ◽  
Drug Candidates ◽  
Hela Cell Lines ◽  
Allium Cepa L

Cancer is a significant global health problem today and responsible for most deaths after cardiovascular diseases. One of the conventional modalities for cancer treatment is chemotherapy. However, it has many side effects and complications. Therefore, the search for novel drug candidates from natural ingredients that are more effective and have minimal side effects is critical. This study is aimed to determine the cytotoxic effect of Allium cepa L. extract on human cervical cancer cells. The cytotoxicity test procedure was performed using MTT assay against HeLa cells. The result revealed that Allium cepa L. ethanolic extract has a weak cytotoxic effect on HeLa cells with IC50 of 900.88 µg/ml. Further research into Allium cepa L. is required to isolate and identify its components and determine their potential as new agents against HeLa cells.

scholarly journals Cytogenotoxic potential of Terminalia actinophylla (Mart.) bark in Allium cepa L.

2020 ◽  
Vol 9 (9) ◽  
pp. e353997322
Author(s):  
Francisca Mairana Silva de Sousa ◽  
José Rafael da Silva Araujo ◽  
Luciana Maria Fortes Castelo Branco Couto ◽  
Regina Maria Silva Sousa ◽  
Maria das Dores Alves Oliveira ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Allium Cepa L ◽  
Phytochemical Profile ◽  
Chromosomal Changes ◽  
Harmful Effects ◽  
Total Average

Terminalia actinophylla (Mart), commonly known as "chapada", is used in folk medicine for the treatment of diarrhea, diabetes and anti-inflammatory. However, studies on its toxicity are still incipient. The present work was carried out with the objective of evaluating the phytochemical profile and the cytogenotoxic effect of the ethanolic extract of the T. actinophylla bark using the Allium cepa L. seed. Seeds of A. cepa were subjected to different extract concentrations (1.25, 2.5, 5 and 10 mg/mL), a negative control (NC - distilled water) as well as trifluralin (PC - positive control), during 24 h of exposure. The phytochemical profile of the extract was obtained to identify the main secondary metabolites. The cytotoxicity (mitotic index) and the genotoxicity (chromosomal alterations) were analyzed by means of the collection of 5,000 meristematic cells. The statistical analysis was carried out using the Kruskal-Wallis test (p<0.05). The phytochemicals present in the extract were tannins, saponins and reducing sugars. Only the lowest concentration (1.25 mg/ml) of T. actinophylla was cytotoxic in comparison with NC. There was a significant increase in the total average of chromosomal changes to lower concentrations (1.25 and 2.5 mg/ml) compared to NC. Probably, phytochemicals in the extract interfere with the cell cycle and cause DNA damage in A. cepa in lower concentrations. However, if bad studies must be carried out on mammals, since the extract with medicinal purpose can have harmful effects on the organism.

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