The Cytotoxicity of Ethanolic Extract of Allium cepa L. on Hela Cell Lines

2021 ◽  
pp. 4969-4972
Author(s):  
Annise Proboningrat ◽  
Shara Jayanti ◽  
Amaq Fadholly ◽  
Arif N. M. Ansori ◽  
Naimah Putri ◽  
...  
Keyword(s):  
Side Effects ◽  
Allium Cepa ◽  
Hela Cells ◽  
Cytotoxic Effect ◽  
Test Procedure ◽  
Ethanolic Extract ◽  
Cytotoxicity Test ◽  
Drug Candidates ◽  
Hela Cell Lines ◽  
Allium Cepa L

Cancer is a significant global health problem today and responsible for most deaths after cardiovascular diseases. One of the conventional modalities for cancer treatment is chemotherapy. However, it has many side effects and complications. Therefore, the search for novel drug candidates from natural ingredients that are more effective and have minimal side effects is critical. This study is aimed to determine the cytotoxic effect of Allium cepa L. extract on human cervical cancer cells. The cytotoxicity test procedure was performed using MTT assay against HeLa cells. The result revealed that Allium cepa L. ethanolic extract has a weak cytotoxic effect on HeLa cells with IC50 of 900.88 µg/ml. Further research into Allium cepa L. is required to isolate and identify its components and determine their potential as new agents against HeLa cells.

scholarly journals Assessment of phytoremediation potencial of Allium cepa L. in raw sewage treatment

2020 ◽  
Vol 80 (2) ◽  
pp. 431-436 ◽  
Author(s):  
A. S. Bertan ◽  
F. P. Baumbach ◽  
I. B. Tonial ◽  
T. S. Pokrywiecki ◽  
E. Düsman
Keyword(s):  
Allium Cepa ◽  
Sewage Treatment ◽  
Oxygen Demand ◽  
Mitotic Cell ◽  
Artemia Salina ◽  
Cytotoxicity Test ◽  
Nitrogen And Phosphorus ◽  
Toxic Activity ◽  
Allium Cepa L ◽  
Physicochemical Data

Abstract Whereas the effects of the substances found in domestic sewage on live organisms is important to evaluate the use of plants to remove pollutants from the environment. The objective of this study was to assess the phytoremediation activity of Allium cepa L. (onion) roots exposed to raw sewage, as well as the acute toxic activity of this effluent for the bioindicators A. cepa, through a cytotoxicity test, and Artemia salina L., through a mortality/immobility test. The physicochemical assessments of the sewage were conducted in two scenarios: immediately after collection and after being in contact with onion roots (phytoremediation) for 24 hours. The physicochemical data indicate there was a reduction in nitrogen and phosphorus levels and in biochemical oxygen demand in sewage treated. The results from the cytotoxicity test with A. cepa indicated a reduction in the mitotic cell divisions of the onions treated with the raw sewage. The mortality/immobility test with A. salina indicated that the concentrations with 50 and 100% of raw sewage induced the mortality of the nauplii. Thus, the data suggest new studies that seek greater efficiency, efficacy and viability of onion phytoremediation.

Cytotoxic Effect of Allium cepa L. Extract on Human colon Cancer (WiDr) Cells: In Vitro Study

2019 ◽  
Vol 12 (7) ◽  
pp. 3483
Author(s):  
Amaq Fadholly ◽  
Arif N. M. Ansori ◽  
Shara Jayanti ◽  
Annise Proboningrat ◽  
Muhammad K. J. Kusala ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Allium Cepa ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Human Colon ◽  
Vitro Study ◽  
Human Colon Cancer ◽  
Allium Cepa L

scholarly journals The potency of chitosan-based Pinus merkusii bark extract nanoparticles as anti-cancer on HeLa cell lines

2019 ◽  
Vol 12 (10) ◽  
pp. 1616-1623
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Regina Purnama Dewi Iskandar ◽  
Agung Budianto Achmad ◽  
Fedik Abdul Rantam ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Hela Cell ◽  
Hela Cells ◽  
Therapeutic Potential ◽  
Bark Extract ◽  
Cytotoxicity Test ◽  
Caspase 9 ◽  
Hela Cell Lines ◽  
Pinus Merkusii

Background and Aim: Cervical cancer accounts for the fourth as a cause of death from cancer in women worldwide, with more than 85% of events and deaths occurring in developing countries. The main problems of chemotherapy are the lack of selectivity and drug resistance. This study aimed to investigate the signal transduction of chitosan-based Pinus merkusii bark extract nanoparticles (Nano-PMBE) as an anticancer on HeLa cell line. Materials and Methods: Nano-PMBE was prepared based on the ionic gelation method. Its anticancer activities in HeLa cells were investigated through cytotoxicity test, cell cycle, and apoptosis analysis. The expression of p53 and caspase-9 was also observed. Results: The results showed that Nano-PMBE has a size of 394.3 nm. Meanwhile, the Nano-PMBE was cytotoxic to HeLa cells ( IC50 of 384.10 μg/ml), caused G0/G1 phase arrest and cell apoptosis in HeLa cells. Besides, the expression of p53 and caspase-9 has increased. Conclusion: The results showed a notable anticancer effect of Nano-PMBE by arresting the cell cycle and inducing apoptosis in HeLa cells, suggesting that it might have therapeutic potential for cervical cancer. Further research is needed to find out more about the anticancer mechanism of Nano-PMBE in HeLa cells to in vivo and clinical studies.

scholarly journals Phytochemical Screening and Anti-Hyperuricemia Activity Test In Vivo of Ethanolic Extract of Shallot (Allium cepa L.) Skin

2020 ◽  
Vol 3 (3) ◽  
pp. 146-151
Author(s):  
Syahrina Syahrina ◽  
Vivi Asfianti ◽  
Kasta Gurning ◽  
Iksen Iksen
Keyword(s):  
Uric Acid ◽  
Allium Cepa ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Purine Metabolism ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Allium Cepa L ◽  
Potassium Oxonate

Uric acid is the final product of purine metabolism that will be excreted through urine, feces, and sweat. Excessive production of uric acid can cause hyperuricemia, known as gout. The skin of shallots (Allium cepa L.) is one of the household wastes that are very rarely used by the community. Ethanol extract of shallot skin (EESS) was tested for phytochemical screening and anti-hyperuricemia activity using potassium oxonate. Mice were divided into five groups (Allopurinol, Na-CMC, EESS 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW) and uric acid levels were observed at 2-hour intervals for six hours. Phytochemical screening shows that EESS has potential compounds in the treatment of gout. Tests to reduce uric acid levels showed that EESS has better potential than allopurinol at concentrations of 300 mg/kg BW and 400 mg/kg BW after six hours of induction in reducing uric acid levels.

scholarly journals TOTAL PHENOLIC CONTENTS, ANTIOXIDANT, ANTICANCER AND ANTIDIABETIC PROPERTIES OF Myrmecodia tuberosa (RUBIACEAE)

2013 ◽  
Vol 9 (3) ◽  
pp. 2000-2004
Author(s):  
Saidi Rasemi ◽  
Dr Heng Yen Khong ◽  
Rohaya Ahmad ◽  
Mizaton Hazizul Hasan
Keyword(s):  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Total Phenolic ◽  
Cytotoxicity Test ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Cancer Agent

Myrmecodia tuberosa, locally known as “Sarang Semut”, belongs to the Rubiaceae family. The aim of the study is to investigate the total phenolic contents and bioactivity of the crude extract of M. tuberosa. The tuber, bark and leaves of the plant were cut, washed and air-dried. The plant was extracted with ethyl acetate and ethanol to yield EtOAc and EtOH crudes. The crudes were then tested for antioxidant 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay, α-glucosidase assay for anti-diabetic activity and [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] MTT Assay for cytotoxicity test. The results showed that bark ethanolic extract demonstrated the highest DPPH scavenging of 95.16% while other crudes were ranging from 92.77% to 94.55% scavenging of DPPH compared to ascorbic acid. The tuber ethyl acetate extract demonstrated the highest inhibition of α -glucosidase enzyme with 72.58% while other crudes were ranging from 63.27% to 72.46% inhibition. In addition, the ethanolic tuber extract exhibited the strongest cytotoxicity against Human colorectal Cancer (HT-29) and Cervical Cancer (HeLa) cell lines with the IC50 value of 16 µg/mL and 14 µg/mL respectively. Meanwhile, the ethanolic bark extract exhibited the strongest cytotoxicity against Human Breast Cancer (MCF-7) with IC50 value of 6.0µg/mL. Furthurmore, the tuber extracts contained the highest phenolic content with 1087mg GAE/ g extract compared to the leaves and barks of the plants. The findings suggest that the whole part of M. tuberosa is a potential natural source for anti-oxidative, anti-diabetic and anti-cancer agent.

scholarly journals Assessment of Preservative Ability of Aqueous and Ethanolic Extracts of Onion (Allium cepa L ) on Soybean Daddawa- A Condiment

2020 ◽  
Vol 8 (3) ◽  
pp. 651
Author(s):  
Adelodun Kolapo ◽  
Temitope Popoola ◽  
Flora Oluwafemi
Keyword(s):  
Allium Cepa ◽  
Ethanolic Extract ◽  
Titratable Acidity ◽  
Absorption Capacity ◽  
Water Absorption Capacity ◽  
Fat Absorption ◽  
Allium Cepa L ◽  
Ethanolic Extracts ◽  
Key Factor ◽  
Glycine Max L

Reports have indicated that lipid peroxidation is a key factor in soybean daddawa deterioration and Alliums are rich in antioxidants. The present study was carried out to investigate the preservative abilities of aqueous and ethanolic extracts of Onion (Allium cepa L) on stored soybean daddawa compared with Sodium Chloride treated samples. Soybean (Glycine max (L). Merr.) seeds fermented into soybean daddawa was preserved with both extracts (at 3, 5 and 7% concentration) and NaCl (at the concentrations of 0, 3 and 5%). The samples stored at 30±2°C for up to 14 days were analyzed for peroxide value (POV), free fatty acid (FFA), pH, titratable acidity (TTA), water absorption capacity (WAC) and fat absorption capacity (FAC). Type of solvent used in extraction did not have significant effect on all the storage indices. Extracts of Onion significantly lowered the POV, FFA and TTA generated in stored soybean daddawa. The pH of stored soybean daddawa samples treated with the NaCl and aqueous extract was not significantly affected while the ethanolic extract significantly reduced the pH from 8.26 – 8.34 to 5.72 – 5.89. The tested extracts lowered the WAC significantly while the FAC of treated soybean daddawa was not significantly affected compared to those treated with NaCl. Results of this study indicate that both extracts of Onion are more effective in lowering peroxidation in stored soybean daddawa and thus could be exploited for soybean daddawa preservation.

scholarly journals Antioxidant potential and α-glucosidase inhibitory activity of onion (Allium cepa L.) peel and bulb extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
S. Masood ◽  
A. ur Rehman ◽  
M. A. Ihsan ◽  
K. Shahzad ◽  
M. Sabir ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Onion Bulb ◽  
Onion Peel ◽  
Allium Cepa L ◽  
Food Applications

Abstract Allium cepa L. is a commonly consumed vegetable that belongs to the Amaryllidaceae family and contains nutrients and antioxidants in ample amounts. In spite of the valuable food applications of onion bulb, its peel and outer fleshy layers are generally regarded as waste and exploration of their nutritional and therapeutic potential is still in progress with a very slow progression rate. The present study was designed with the purpose of doing a comparative analysis of the antioxidant potential of two parts of Allium cepa, i.g., bulb (edible part) and outer fleshy layers and dry peels (inedible part). Moreover, the inhibitory effect of the onion bulb and peel extracts on rat intestinal α-glucosidase and pancreatic α-amylase of porcine was also evaluated. The antioxidant potential of onion peel and bulb extracts were evaluated using 2,2-diphenyl- 1-picryl hydrazyl (DPPH), ferric-reducing antioxidant power assay (FRAP), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay, H2O2 radical scavenging activity and Fe2+ chelating activity. Total flavonoids and phenolic content of ethanolic extract of onion peel were significantly greater as compared to that of onion bulb. Ethanolic extract of onion peel also presented better antioxidant and free-radical scavenging activity as compared to the ethanolic extract of bulb, while the aqueous extract of bulb presented weakest antioxidative potential. Onion peel extract’s α-glucosidase inhibition potential was also correlated with their phenolic and flavonoid contents. The current findings presented onion peel as a possible source of antioxidative agents and phenolic compounds that might be beneficial against development of various common chronic diseases that might have an association with oxidative stress. Besides, outer dry layers and fleshy peels of onion exhibited higher phenolic content and antioxidant activities, compared to the inner bulb. The information obtained by the present study can be useful in promoting the use of vegetable parts other than the edible mesocarp for several future food applications, rather than these being wasted.

scholarly journals Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells

2010 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Raditya Prima Istiaji ◽  
Maya Fitria ◽  
Larasati Larasati ◽  
Fortunella Tjondro ◽  
Astrid Ayu Maruti ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Side Effects ◽  
Cancer Cells ◽  
Hela Cells ◽  
Human Cancer ◽  
Ethanolic Extract ◽  
Solanum Nigrum ◽  
Human Cancer Cells ◽  
Cervical Cancer Cells ◽  
Solanum Nigrum L

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value). The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed the effect of synergism combination. The Ooptimum concentration of combination was 1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored. Key words: Leunca (Solanum nigrum L.), cisplatin, cytotoxic, combination agent, HeLa cells

scholarly journals Cytological changes in meristematic cells of Allium cepa L. root tip treated with extracts from callus of Catharanthus roseus (L.) G. Don

2014 ◽  
Vol 68 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Agnieszka Pietrosiuk ◽  
Mirosława Furmanowa ◽  
Alicja Zobel ◽  
Mieczysław Kuraś ◽  
Agnieszka Michalak
Keyword(s):  
Allium Cepa ◽  
Catharanthus Roseus ◽  
Ethanolic Extract ◽  
Root Tip ◽  
Root Cells ◽  
Irregular Structures ◽  
Allium Cepa L ◽  
Green Callus ◽  
Pharmacologically Active ◽  
Tip Cells

The effect of an ethanolic extract from callus of <em>Catharanthus roseus</em> on <em>Allium cepa</em> root cells divisions was investigated. Two lines: white and green callus, were established on solid B5 medium with IAA 1 mg/l and kinetin 0.1 mg/l. The HPLC analysis of callus extracts showed the presence of indole alkaloids, however not known pharmacologically active alkaloids or derivatives used in semisynthesis (vinblastine, ajmalicine, serpentine, yohimbine, vindoline and catharanthine) have been found. The ethanolic extract of <em>C. roseus</em> callus inhibited the number of mitoses in <em>Allium cepa</em> root tip cells. Short (1-3 hours) treatment resulted in an increase in the index of late prophases, with characteristic light spaces, and the index of metaphases with twisted chromosomes forming an equatorial plate or irregular structures of c-metaphases. At the same time, the percentage of anaphases and telophases decreased significantly. Longer treatment of the root tip inhibited mitotic activity, stopping it completely already after 12 hours. Interphase nuclei became at first denser and homogeneous, eventually their structure became , partitioned into zones and formation of chromatin territories, with distinct large nucleoli has been observed. Electron microscope observations revealed well developed rough endoplasmic reticulum and thick, invaginating cell membrane.

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