scholarly journals Characterization of airborne fungi present in two hospitals in Kabale district – Uganda

2020 ◽  
Vol 101 ◽  
pp. 392-393
Author(s):  
A. Odebode ◽  
G. Nimawanya
Keyword(s):  
Airborne Fungi ◽  
Kabale District

scholarly journals Sampling and Characterization of the Environmental Fungi in the Provincial Historic Archive of Pinar Del Río, Cuba

2020 ◽  
Vol 1 (8) ◽  
pp. 404-420
Author(s):  
Sofia Borrego ◽  
Alian Molina ◽  
Tamara Abrante
Keyword(s):  
Respiratory Tract ◽  
Human Health ◽  
Culture Media ◽  
Airborne Fungi ◽  
New Findings ◽  
Del Rio ◽  
Positive Results ◽  
Made In

It has been reported that there is a correlation between indoor airborne fungi and the biodeterioration of valuable documents in archives, libraries and museums, and that these fungi can also cause effects on human health if there are immunological problems or the time of exposure to these environments of low quality is long. The aims of this study were quantifying and characterizing the mycobiota of the indoor air in three repositories of the Provincial Historical Archive of Pinar del Río, Cuba and assessing its impact on the human health. The samplings were made in two different months corresponding to the years 2016 and 2017, one belonging to the rainy season and the other to the season of the little rain using a SAS biocollector and appropriate culture media to isolate fungi. The fungal concentrations and the Indoor/Outdoor (I/O) ratios obtained revealing that the repositories showed good quality environments. In both isolations Cladosporium was the predominant genus followed by Penicillium in the first sampling and Fusarium in the second isolation. The genera Aureobasidium, Sepedonium, Trichaegum and Wallemia were new findings for the Cuban archives. The pathogenic attributes studied showed that 30% of the isolates have spores so small that they can penetrate into the respiratory tract into the alveoli; 10.7% of the taxa obtained in the first isolation and 13.3% of the taxa detected in the second sampling also showed positive results to four virulence tests analyzed "In vitro" (growth at 37°C, hemolytic activity, phospholipase activity and respiratory tract level to which the spores can penetrate). These virulence factors (pathogenic attributes) evidence the risk that environmental fungi represent for the health of personnel in this archive.

scholarly journals How Working Tasks Influence Biocontamination in an Animal Facility

2019 ◽  
Vol 9 (11) ◽  
pp. 2216 ◽  
Author(s):  
Anna M. Marcelloni ◽  
Alessandra Chiominto ◽  
Simona Di Renzi ◽  
Paola Melis ◽  
Annarita Wirz ◽  
...  
Keyword(s):  
Airborne Fungi ◽  
Airborne Microorganisms ◽  
Air Sampler ◽  
Fel D 1 ◽  
Inhalable Dust ◽  
Storage Area ◽  
Can F 1 ◽  
High Level ◽  
Determinant Factor

The exposure to biocontaminants in animal facilities represents a risk for developing infectious, allergic and toxic diseases. The aim of this study was to determine what factors could be associated with a high level of exposure to biological agents through the measure and characterization of airborne fungi, bacteria, endotoxin, (1,3)-β-d-glucan and animal allergens. Airborne microorganisms were collected with an air sampler and identified by microscopic and biochemical methods. Endotoxin, (1,3)-β-d-glucan, Mus m 1, Rat n 1, Can f 1, Fel d 1, Equ c 4 allergens were detected on inhalable dust samples by Kinetic LAL, Glucatell, and ELISA assays, respectively. Our data evidenced that changing cages is a determinant factor in increasing the concentration of the airborne biocontaminants; the preparation of bedding and distribution of feed, performed in the storage area, is another critical working task in terms of exposure to endotoxins (210.7 EU/m3) and (1,3)-β-d-glucans (4.3 ng/m3). The highest concentration of Mus m 1 allergen (61.5 ng/m3) was observed in the dirty washing area. The detection of expositive peaks at risk of sensitization (>2 μg/g) by Fel d 1 in animal rooms shows passive transport by operators themselves, highlighting their role as vehicle between occupational and living environments.

scholarly journals High levels of primary biogenic organic aerosols in the atmosphere in summer are driven by only a few microbial taxa from the leaves of surrounding plants

2020 ◽  
Author(s):  
Abdoulaye Samaké ◽  
Aurélie Bonin ◽  
Jean-Luc Jaffrezo ◽  
Pierre Taberlet ◽  
Samuël Weber ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Physicochemical Characterization ◽  
Organic Aerosols ◽  
Sampling Period ◽  
Airborne Fungi ◽  
Soil Samples ◽  
Atmospheric Particulate Matter ◽  
Surrounding Environment ◽  
Bacteria And Fungi

Abstract. Primary biogenic organic aerosols (PBOA) represent a major fraction of coarse organic matter (OM) in air. Despite their implication in many atmospheric processes and human health problems, we surprisingly know little about PBOA characteristics (i.e., composition, dominant sources, and contribution to airborne-particles). In addition, specific primary sugar compounds (SCs) are generally used as markers of PBOA associated with Bacteria and Fungi but our knowledge of microbial communities associated with atmospheric particulate matter (PM) remains incomplete. This work aimed at providing a comprehensive understanding of the microbial fingerprints associated with SCs in PM10 (particles smaller than 10 µm) and their main sources in the surrounding environment (soils and vegetation). An intensive study was conducted on PM10 collected at rural background site located in an agricultural area in France. We combined high-throughput sequencing of Bacteria and Fungi with detailed physicochemical characterization of PM10, soils and plant samples, and monitored meteorology and agricultural activities throughout the sampling period. Results shows that in summer SCs in PM10 are a major contributor of OM in air, representing 0.8 to 13.5 % of OM mass. SCs concentrations are clearly determined by the abundance of only a few specific airborne Fungi and Bacteria Taxa. These microbial are significantly enhanced in leaf over soil samples. Interestingly, the overall community structure of Bacteria and Fungi are similar within PM10 and leaf samples and significantly distinct between PM10 and soil samples, indicating that surrounding vegetation are the major source of SC-associated microbial taxa in PM10 in rural area.

Molecular characterization of airborne fungi in caves of the Mogao Grottoes, Dunhuang, China

2011 ◽  
Vol 65 (5) ◽  
pp. 726-731 ◽  
Author(s):  
Wanfu Wang ◽  
Xu Ma ◽  
Yantian Ma ◽  
Lin Mao ◽  
Fasi Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Airborne Fungi ◽  
Mogao Grottoes

scholarly journals Characterization of allergens and airborne fungi in low and middle-income homes of primary school children in Durban, South Africa

2012 ◽  
Vol 55 (12) ◽  
pp. 1110-1121 ◽  
Author(s):  
Nkosana Jafta ◽  
Stuart A. Batterman ◽  
Nceba Gqaleni ◽  
Rajen N. Naidoo ◽  
Thomas G. Robins
Keyword(s):  
South Africa ◽  
Primary School ◽  
School Children ◽  
Airborne Fungi ◽  
Primary School Children ◽  
Middle Income ◽  
Low And Middle Income

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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