Sampling and Characterization of the Environmental Fungi in the Provincial Historic Archive of Pinar Del Río, Cuba

It has been reported that there is a correlation between indoor airborne fungi and the biodeterioration of valuable documents in archives, libraries and museums, and that these fungi can also cause effects on human health if there are immunological problems or the time of exposure to these environments of low quality is long. The aims of this study were quantifying and characterizing the mycobiota of the indoor air in three repositories of the Provincial Historical Archive of Pinar del Río, Cuba and assessing its impact on the human health. The samplings were made in two different months corresponding to the years 2016 and 2017, one belonging to the rainy season and the other to the season of the little rain using a SAS biocollector and appropriate culture media to isolate fungi. The fungal concentrations and the Indoor/Outdoor (I/O) ratios obtained revealing that the repositories showed good quality environments. In both isolations Cladosporium was the predominant genus followed by Penicillium in the first sampling and Fusarium in the second isolation. The genera Aureobasidium, Sepedonium, Trichaegum and Wallemia were new findings for the Cuban archives. The pathogenic attributes studied showed that 30% of the isolates have spores so small that they can penetrate into the respiratory tract into the alveoli; 10.7% of the taxa obtained in the first isolation and 13.3% of the taxa detected in the second sampling also showed positive results to four virulence tests analyzed "In vitro" (growth at 37°C, hemolytic activity, phospholipase activity and respiratory tract level to which the spores can penetrate). These virulence factors (pathogenic attributes) evidence the risk that environmental fungi represent for the health of personnel in this archive.

Download Full-text

Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽

10.1007/s11306-016-1029-2 ◽

2016 ◽

Vol 12 (5) ◽

Cited By ~ 4

Author(s):

Érika Cristina dos Santos ◽

Camila Bruna de Lima ◽

Kelly Annes ◽

Marcella Pecora Milazzotto

Keyword(s):

Culture Media ◽

Bovine Embryos ◽

In Vitro Production ◽

Noninvasive Characterization ◽

Vitro Production

Download Full-text

Applicability and Limitations in the Characterization of Poly-Dispersed Engineered Nanomaterials in Cell Media by Dynamic Light Scattering (DLS)

Materials ◽

10.3390/ma12233833 ◽

2019 ◽

Vol 12 (23) ◽

pp. 3833 ◽

Cited By ~ 2

Author(s):

Arianna Marucco ◽

Elisabetta Aldieri ◽

Riccardo Leinardi ◽

Enrico Bergamaschi ◽

Chiara Riganti ◽

...

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Culture Media ◽

Raw 264.7 ◽

Pre Treatment ◽

The Stability ◽

Definition Of ◽

Cellular Tests

The dispersion protocol used to administer nanomaterials (NMs) in in vitro cellular tests might affect their toxicity. For this reason, several dispersion procedures have been proposed to harmonize the toxicological methods, allowing for the comparison of the data that were obtained by different laboratories. At the same time, several techniques and methods are available to monitor the identity of the NMs in the cell media. However, while the characterization of suspensions of engineered NMs having narrow size distribution may be easily performed, the description of aggregated NMs forming polydispersions is still challenging. In the present study, sub-micrometric/nanometric TiO2, SiO2, and CeO2 were dispersed in cell media by using two different dispersion protocols, with and without albumin (0.5%) and with different sonication procedures. Dynamic Light Scattering (DLS) was used to characterize NMs in stock solutions and culture media. Pitfalls that affect DLS measurements were identified and, guidance on a critical analysis of the results provided. The NMs were then tested for their cytotoxicity (LDH leakage) toward murine macrophages (RAW 264.7) and PMA-activated human monocytes (THP-1). As markers of pro-inflammatory response, nitric oxide (NO) and cytokine IL-1β production were measured on RAW 264.7 and THP-1 cells, respectively. The pre-treatment with albumin added to a strong sonication treatment increases the stability and homogeneity of the suspensions of nanometric samples, but not of the submicrometric-samples. Nevertheless, while TiO2 and CeO2 were non-cytotoxic in any conditions, differences in cytotoxicity, NO, and IL-1β releases were found for the SiO2, depending upon the protocol. Overall, the results suggest that there is no one-fits-all method valid for all NMs, since each class of NMs respond differently. The definition of validated procedures and parameters for the selection of the most appropriate method of dispersion for each class of NM appears to be a more efficacious strategy for the harmonization of the dispersion protocols.

Download Full-text

Isolation and Characterization of Fusarium moniliforme var. subglutinans from Malformed Mango

Journal of Agricultural and Marine Sciences [JAMS] ◽

10.24200/jams.vol4iss2pp19-25 ◽

1999 ◽

Vol 4 (2) ◽

pp. 19

Author(s):

K.P. Akhtar ◽

M. Asif ◽

M.A. Khan ◽

M.J. Jaskani ◽

I.A. Khan

Keyword(s):

Fusarium Moniliforme ◽

Culture Media ◽

Isolation And Characterization ◽

Ph Conditions ◽

Fungal Association ◽

Mango Malformation ◽

Growth Characters ◽

Better Than

Mango malformation occurs in most mango growing regions of the world. Floral and vegetative malformation have been reported. There is general agreement that the fungal pathogen Fusarium moniliforme var. subglutinans or Fusarium subglutinans is the causal agent. Healthy and malformed samples of both floral and vegetative tissues were collected from different varieties of mango grown in several locations to verify the association of F.moniliforme with mango malformation disease in Pakistan. The fungus was isolated and cultured. Frequency of fungal association with the disease ranged between 90- 94%, There was less recovery of fungus from asymptomatic tissue (12- 15%). There was no difference among the commercial mango varieties in the level of susceptibility to this disease. However, seedling germplasm and land races showing resistance to mango malformation were identified. The in vitro growth characters of the fungus were determined on different culture media, at varying temperatures, light and pH conditions. Mycelial growth on potato dextrose agar was better than nine other media tested. At pH 7.00, the ideal temperature for growth was between 25-30° C. Normally, the malformation is not controlled by fungicide application. The in vitro sensitivity of fungus to six fungicides at three concentrations was determined to seek potential means of chemical control.

Download Full-text

100 A Comparison of Two Media on Growth of Deciduous Azalea Shoots In Vitro

HortScience ◽

10.21273/hortsci.34.3.458e ◽

1999 ◽

Vol 34 (3) ◽

pp. 458E-458

Author(s):

Carol D. Robacker ◽

Betty Robicheaux

Keyword(s):

Woody Plant ◽

Culture Media ◽

Shoot Tips ◽

Fresh Medium ◽

First Flush ◽

Wide Range ◽

Woody Plant Medium ◽

Made In

Micropropagation is a useful technique to propagate species such as deciduous azaleas, which are difficult to root from cuttings. To develop a micropropagation protocol that would be effective with a wide range of species and cultivars of native azalea, two culture media, Woody Plant Medium (WPM) (Lloyd and McCown, 1980) and ER medium (Economou and Read, 1994) were evaluated for ability to support growth of 11 species and four cultivars of deciduous azalea. Shoot tips were obtained from the first flush of growth in the spring on plants growing in the greenhouse or field. Following disinfection, the terminal and basal ends were removed from each explant. The explants were placed in culture tubes containing either WPM or ER medium with 12 mg/L 2iP and solidified with agar. Cultures were transferred to fresh medium every 4 to 6 weeks. Initial evaluations were made in 1996, and the experiment was repeated in 1997. In 1998, six of the taxa were evaluated for a third year. For most of the taxa evaluated, growth was superior on ER medium. On WPM, many of the cultures browned and died. R. canescens, R. viscosum, R. prunifolium, and R. austrinum are examples of species that preferred ER medium. R. alabamense, R. arborescens, and `My Mary' performed similarly on either medium.

Download Full-text

Characterization of polypeptides made in vitro from bacteriophage lambda DNA

Journal of Molecular Biology ◽

10.1016/0022-2836(73)90281-7 ◽

1973 ◽

Vol 78 (4) ◽

pp. 589-600 ◽

Cited By ~ 29

Author(s):

Bernard Allet ◽

Kazuo J. Katagiri ◽

Raymond F. Gesteland

Keyword(s):

Bacteriophage Lambda ◽

Lambda Dna ◽

Made In

Download Full-text

Dextranomer/Hyaluronic Acid Copolymer (Dx/HA) Has no Effect on Bacterial Growth in Culture Media With or Without Antibiotic Discs

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v9i2.715 ◽

2020 ◽

Vol 9 (2) ◽

Author(s):

Karabulut R ◽

Doğruman-Al F ◽

Türkyılmaz Z ◽

Sönmez K ◽

Demirel F ◽

...

Keyword(s):

Hyaluronic Acid ◽

Bacterial Growth ◽

Culture Media ◽

Acid Copolymer ◽

Mueller Hinton ◽

Mueller Hinton Agar ◽

Agar Media ◽

Inhibition Zones ◽

Made In

Introduction: Endoscopic treatment of vesicoureteral refl ux (VUR) by subureteral injection of biocompatible polymers is an established treatment option for refl ux. Dextranomer/hyaluronic acid copolymer(Dx/HA) has gained wide popularity for treating VUR. We decided to investigate the antibacterial activity of Dx/HA and its interaction with antibiotics in in-vitro conditions. Materials and Methods: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus mirabilis suspensions were inoculated into Mueller-Hinton agar media and 30 μl of Dx/HA was inoculated in 5 mm diameter pits and the plates were incubated at 37°C for 24 hours. At the end of the incubation period, inhibition zones around the discs were measured. Expansion of the inhibition zones towards the pits which contained Dx/HA was considered as synergism. Dx/ HA was inoculated into pits made in Mueller-Hinton agar medium without antibiotic discs but containing suspensions of bacteria. These media were incubated under the same circumstances and same measurements were done. All experimental procedures were performed twice. Increase in bacterial zone diameters for ≥ 5 mm was inoculated was regarded as signifi cant for each agent. Results: Dx/HA caused no difference in bacterial growth either with or without antibiotic discs as determined by inhibition zones in the culture media. Conclusion : Dx/ha will not contribute to UTI if it is used for the treatment of VUR in cases either with or without infection.

Download Full-text

Bisphenol A Analogues: A Brief Review of their Occurrence in Food, Biological Samples and Endocrine Effects

Archives of Ecotoxicology ◽

10.36547/ae.2020.2.4.89-94 ◽

2020 ◽

Vol 2 (4) ◽

pp. 89-94

Author(s):

Nikola Knizatova ◽

Katarína Tokárová ◽

Hana Greifová ◽

Tomáš Jambor ◽

Peter Massányi ◽

...

Keyword(s):

Bisphenol A ◽

Human Health ◽

Human Exposure ◽

Food Packaging ◽

In Vitro Tests ◽

Endocrine Disrupting Chemical ◽

Endocrine Disrupting ◽

Made In

Bisphenol A (BPA) is the most well-known compound from the bisphenol family. There is increasing evidence that bisphenol BPA used in plastics, receipts, food packaging, and other products might be harmful to human health due to its actions as an endocrine-disrupting chemical, therefore BPA is being replaced by compounds very similar in structure, but data on the occurrence and effects of these BPA analogs are limited. Therefore, there is increasing concern regarding human exposure to bisphenol analogs (BPs) due to their widespread use and potential adverse effects. The main objective of this work was to investigate human exposure to BPs and the associated endocrine activities. We performed a literature review of the available research made in humans, in in vivo and in vitro tests. The findings support the idea that exposure to BPs may have an impact on human health, especially in terms of endocrine disruption.

Download Full-text

Characterization of pseudarthrosis with ultrasound backscattered signals in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000100005 ◽

2010 ◽

Vol 25 (1) ◽

pp. 13-17 ◽

Cited By ~ 2

Author(s):

Manoel Luiz Ferreira ◽

Daniel Patterson Matusin ◽

Christiano Bittencourt Machado ◽

Paulo César Silva ◽

Natalia Bernades Mello ◽

...

Keyword(s):

Small Animal ◽

Control Group ◽

Ultrasound Backscatter ◽

Group A ◽

Backscattered Signals ◽

Novel Model ◽

Experimental Group ◽

Made In

PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the ability of backscatter parameters from ultrasound signals in differentiating normal bone from those ones with pseudarthorsis. METHODS: Twelve Rattus norvegicus albinus free from pathogenic species (SPF) were randomly divided in two groups, with six animals each. In the Control group a surgical approach to the femur was made, followed by the synthesis of the muscle and skin layers. The Experimental group was submitted to an osteotomy of the femur and a vascularized flap of the fascia lata was interposed in the line of the fractured bone. Then the alignment and bone stabilization were accomplished, by using nylon stitch in U shape introduced in holes made in the proximal and distal fractured bone. Bone samples were scanned with ultrasound and signals were collected for each one to analyze the parameter Apparent Integrated Backscatter - AIB. RESULTS: Radiological and anatomopathologic studies revealed the absence of bone consolidation with persistence of fiber-osteoid tissue. Values of the ultrasound parameter AIB from normal bones were statistically different from those with pseudarthrosis. CONCLUSION: The experimental model was suitable for pseudarthrosis development in rats and the ultrasound backscatter parameters were able to identify such a bone disease in vitro.

Download Full-text

Bisphenol A Analogues in Food and Their Hormonal and Obesogenic Effects: A Review

Nutrients ◽

10.3390/nu11092136 ◽

2019 ◽

Vol 11 (9) ◽

pp. 2136 ◽

Cited By ~ 17

Author(s):

Andújar ◽

Gálvez-Ontiveros ◽

Zafra-Gómez ◽

Rodrigo ◽

Álvarez-Cubero ◽

...

Keyword(s):

Literature Review ◽

Bisphenol A ◽

Human Health ◽

Health Effects ◽

Human Exposure ◽

In Vitro Tests ◽

Adverse Health Effects ◽

Made In

Bisphenol A (BPA) is the most well-known compound from the bisphenol family. As BPA has recently come under pressure, it is being replaced by compounds very similar in structure, but data on the occurrence of these BPA analogues in food and human matrices are limited. The main objective of this work was to investigate human exposure to BPA and analogues and the associated health effects. We performed a literature review of the available research made in humans, in in vivo and in vitro tests. The findings support the idea that exposure to BPA analogues may have an impact on human health, especially in terms of obesity and other adverse health effects in children.

Download Full-text

Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media

Journal of Nanomaterials ◽

10.1155/2016/5937932 ◽

2016 ◽

Vol 2016 ◽

pp. 1-19 ◽

Cited By ~ 6

Author(s):

Verónica Freyre-Fonseca ◽

Darío I. Téllez-Medina ◽

Estefany I. Medina-Reyes ◽

Maribel Cornejo-Mazón ◽

Edgar O. López-Villegas ◽

...

Keyword(s):

Titanium Dioxide ◽

Culture Media ◽

Titanium Dioxide Nanoparticles ◽

Physicochemical Characterization ◽

Cell Culture Media ◽

Size Stability ◽

Maximum Stability

Titanium dioxide nanoparticles (TiO2NP) are possible carcinogenic materials (2B-IARC) and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2NP by evaluating their morphometry, stability, and zeta potential (ζ) in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS) resulted to be 10–50 times larger than those obtained by digital image analysis (DIA) given the charged zone around particles. Fractal dimension (FD) was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci) which was approximately unchanged with time. All dispersions hadζvalues around −30 mV at physiological pH (7.4) and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis). Results help in understanding the complex NP geometry-size-stability relationships when performingin vivoandin vitroenvironmental-toxicity works and help in supporting decisions on the usage of TiO2NP.

Download Full-text