Simultaneous quantification of oleanolic acid, ursolic acid, betulinic acid and lupeol in different populations of five Swertia species by using HPTLC-densitometry: Comparison of different extraction methods and solvent selection

2019 ◽  
Vol 130 ◽  
pp. 537-546 ◽  
Author(s):  
Prabhjot Kaur ◽  
R.C. Gupta ◽  
Abhijit Dey ◽  
Devendra Kumar Pandey
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Betulinic Acid ◽  
Extraction Methods ◽  
Solvent Selection ◽  
Simultaneous Quantification ◽  
Different Populations ◽  
Swertia Species

Simultaneous Quantification of Ursolic and Oleanolic Acids in Glechoma hederacea and Glechoma hirsuta by UPLC/MS/MS

Planta Medica ◽  
2021 ◽  
Author(s):  
Karolina Grabowska ◽  
Paweł Żmudzki ◽  
Dagmara Wróbel-Biedrawa ◽  
Irma Podolak
Keyword(s):  
Ethyl Acetate ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Dry Weight ◽  
Future Application ◽  
Plant Materials ◽  
Plant Parts ◽  
Simultaneous Quantification ◽  
Aerial Parts ◽  
Glechoma Hederacea

AbstractThe content of ursolic acid and oleanolic acid was determined in different plant parts of two Glechoma species, G. hederacea and G. hirsuta. To achieve optimal extraction conditions of ursolic acid and oleanolic acid from plant material, several methods including maceration, heat reflux, Soxhlet, and ultrasonic extraction, as well as various solvents (methanol, dichloromethane, ethyl acetate), were investigated and compared.For the simultaneous quantification of pentacyclic triterpenes in extracts from Glechoma sp., an UPLC-MS/MS was developed and validated. The method exhibited good linearity, precision, and recovery, and it also was simple, specific, and fast. We developed the method for future application in the quality control of plant materials and botanical extracts containing ursolic acid and oleanolic acid. With regard to the triterpene constituents, both G. hederacea and G. hirsuta can be used equally, and the aboveground parts of both species, but the leaves especially, are abundant sources of ursolic acid (7.1 – 7.5 mg/g dry weight [DW]). Dichloromethane as an extractant provided the best extraction efficiency as well as selectivity to obtain Glechoma extracts rich in triterpenes as compared to methanol and ethyl acetate, regardless of the particular extraction technique. Dry dichloromethane extracts from aerial parts of Glechoma sp. obtained by the heat reflux method resulted in products with a high content of UA (17 – 25% w/w) are considered to be convenient and rich sources of this compound.

scholarly journals Simultaneous Quantification of Bioactive Triterpene acids (Ursolic acid and Oleanolic acid) in Different Extracts of Eucalyptus globulus (L) by HPTLC Method

2017 ◽  
Vol 10 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Arti Gupta ◽  
Pooja Maheta ◽  
Renu Chauhan ◽  
Sonia Pandey ◽  
Jitendra Singh Yadav ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Eucalyptus Globulus ◽  
Simultaneous Quantification ◽  
Triterpene Acids ◽  
Hptlc Method

Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Leaves of Paulownia by HPLC

2013 ◽  
Vol 781-784 ◽  
pp. 787-791 ◽  
Author(s):  
Ya Li Xing ◽  
Liang Wu Bi ◽  
Zhen Dong Zhao ◽  
Tian Juan Xia
Keyword(s):  
Phosphoric Acid ◽  
Simultaneous Determination ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Flow Rate ◽  
Mobile Phase ◽  
Leaf Extract ◽  
Hplc Method ◽  
Simultaneous Quantification

A quick and accurate HPLC method has been developed for the simultaneous quantification of two bioactive triterpenes, ursolic acid and oleanolic acid in Paulownia leaves. The samples were analyzed on a Shim-pack ODS-CLC (M) (4.6 mm × 250 mm, 5 μm) column kept at 21 °C, using the methanol and aqueous phase containing 0.05%phosphoric acid with the volumetric ratio of 91.7:8.3 as the mobile phase at a flow rate of 0.6 mL/ min, and the detection wavelength was set at 210 nm. The method was validated and applied to the simultaneous quantification of the two triterpenes in Paulownia leaf extract. The standard curves were established in the range of 0.44 ~ 8.75 μg for oleanolic acid and 0.92 ~ 18.37 μg for ursolic acid. The contents of oleanolic acid and ursolic acid in leaves of Paulownia were determinated using the HPLC method and the contents were 3.87 mg/g and 13.61 mg/g, respectively.

scholarly journals Chemical Constituents from Fadogia homblei De Wild (Rubiaceae)

2013 ◽  
Vol 14 ◽  
pp. 116-124 ◽  
Author(s):  
Abdelhafeez M.A. Mohammed ◽  
Philip H. Coombes ◽  
Neil R. Crouch ◽  
Dulcie A. Mulholland
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Betulinic Acid ◽  
Chemical Constituents ◽  
First Report ◽  
Plant Source ◽  
Lupane Triterpenoids ◽  
Coumarin 7

The total of fourteen known compounds was isolated from the fruits, leaf and stem of Fadogia homblei De Wild. (Rubiaceae) and identified as: a coumarin, 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one (scopoletin) 1, two flavones; 3′,4′,5,7-tetrahydroxyflavone (luteolin) 2 and quercetin-3-O-β-D-galactoside 3, four lupane triterpenoids; lupeol 4, betulinic acid 5, 3β-dodecanoyllup-20(29)-en-28-al 6, lup-20(29)-en-3β-ylhexadecanoate 7, and two steroids; sitosterol 8, stigmasterol 9, a lignan 4,4′-dihydroxy-3,3′-dimethoxy-7,9′:7′,9-diepoxylignan ((-)-pinoresinol) 10, a phaeophytin A 11, an uracil 12, an oleanolic acid 13, and an ursolic acid 14. To our best knowledge, this is the first report for isolation of these compounds from this species. This finding is nevertheless significant as it is the first report of uracil from a plant source other than from various species of ferns.

scholarly journals A New Hyptadienic Acid Derivative from Hyptis Verticillata (Jacq.) with Insecticidal Activity

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Duanne A. C. Biggs ◽  
Roy B. R. Porter ◽  
William F. Reynolds ◽  
Lawrence A. D. Williams
Keyword(s):  
Acid Derivative ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Insecticidal Activity ◽  
Betulinic Acid ◽  
Spectroscopic Analysis ◽  
Cylas Formicarius ◽  
Sweet Potato Weevil ◽  
Cylas Formicarius Elegantulus ◽  
Tormentic Acid

A new triterpene, A(1)-1-benzoate-19α-hydroxy-urs-2(3),12(13)-dien-28-oic acid (1), was isolated from Hyptis verticillata, in addition to the known triterpenes tormentic acid, isolated as the diacetate (2), betulinic acid (3), α- and β-amyrin, oleanolic acid and ursolic acid. The structure of 1 was elucidated by spectroscopic analysis. Compounds 1–3 exhibited insecticidal activity against the sweet potato weevil Cylas formicarius elegantulus (Summers).

Ursolic Acid and Oleanolic Acid: Pentacyclic Terpenoids with Promising Anti-Inflammatory Activities

2016 ◽  
Vol 10 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Dharambir Kashyap ◽  
Ajay Sharma ◽  
Hardeep S. Tuli ◽  
Sandeep Punia ◽  
Anil K. Sharma
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Anti Inflammatory

Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid

2020 ◽  
Vol 6 (2) ◽  
pp. 134-146 ◽  
Author(s):  
Kehkashan Arshad Qamar ◽  
Ahsana Dar Farooq ◽  
Bina S. Siddiqui ◽  
Nurul Kabir ◽  
Sabira Begum
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Mitotic Spindle ◽  
Immunofluorescence Microscopy ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Ocimum Basilicum ◽  
Antiproliferative Effects ◽  
Ancient Times ◽  
Mcf 7

Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Objective: To emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy. Method: O. basilicum (aerial parts) methanolic extract and fractions were screened against HT-144, MCF-7, NCI-H460 and SF-268 human cancer cell lines using sulforhodamine B assay. The more active Petroleum Ether Insoluble (PEI) fraction was fractionated into six sub-fractions (OB-1 to OB-6). Four pure compounds (3-O-methyl ursolic acid, oleanolic acid, 3-epi-ursolic acid and ursolic acid) were isolated from the more potent sub-fraction OB- 6. Triple channel immunofluorescence microscopy was employed to observe the effects of methanolic extract, PEI fraction, sub-fractions OB-5 and OB-6, 3-epi-ursolic acid and oleanolic acid on the cytoskeleton and nuclei of MCF-7 cells. Result: The methanolic extract and the PEI fraction exhibited selectively greater growth inhibition against MCF-7 cell line (TGI: 56 and 36.2 µg/ml, respectively). By using triple channel immunofluorescence microscopy, it was observed that the methanolic extract, PEI fraction, sub-fraction OB-5 and 3-epi-ursolic acid induced irregular mitotic spindle formation and slowing of mitotic progression in MCF-7 cells while sub-fraction OB-6 induced mitotic arrest in the prophase stage. F-actin aggregation was also visible in PEI fraction, subfraction OB-5 and 3-epi-ursolic acid treated MCF-7 cells. Conclusion: These results emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy and suggest that it inhibits the growth of MCF-7 cancer cells via multiple mechanisms such as interaction with the microtubules and mitotic spindle apparatus, and F-actin aggregation.

Sign in / Sign up

Export Citation Format

Share Document