Comparison Of Total Protein Profile Of Alternaria Alternata Extract Obtained From Various U.S. Allergenic Extract Manufacturers

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.

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Protein Profile of Human Perilymph: In Search of Markers for the Diagnosis of Perilymph Fistula and other Inner Ear Disease

Otolaryngology ◽

10.1177/01945998941113p117 ◽

1994 ◽

Vol 111 (3P1) ◽

pp. 273-280 ◽

Cited By ~ 16

Author(s):

Isolde Thalmann ◽

Robert I. Kohut ◽

Jay Ryu ◽

Thomas H. Comegys ◽

Masamitsu Senarita ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Inner Ear ◽

Total Protein ◽

Gel Electrophoresis ◽

Protein Profile ◽

Polyacrylamide Gel Electrophoresis ◽

Density Lipoprotein ◽

Polyacrylamide Gel ◽

Computer Assisted ◽

Two Dimensional

Recent developments in high-resolution two-dimensional polyacrylamide gel electrophoresis, combined with amino acid sequencing and computer-assisted image analysis, have allowed separation of approximately 100 proteins and identification and quantitation of some 30 proteins in human perilymph. The majority of proteins were found to be present in perilymph at levels in basic agreement with the total protein gradient between perilymph and plasma (1:35). However, several striking differences were observed: (1) β2-transferrin, known to be absent from normal plasma but present in cerebrospinal fluid, was detected in perilymph at a concentration roughly equal to that in cerebrospinal fluid; and (2) two high-density lipoprotein-associated apolipoproteins—apo D (formerly PLS:33) and apo J or NA1 and NA2 (formerly PSL:29/30), the latter showing identity with 5P40/40, or cytolysis inhibitor—were found to be present at concentrations 1 to 2 orders of magnitude higher when examined in terms of total protein and to be comparable with or higher than plasma levels when examined in terms of absolute concentrations. The functional significance of the extremely high levels of the two apolipoproteins is not known at this time. An attempt was made to use β2-transferrin, as well as apo D and apo J (NA1/NA2), as markers for the diagnosis of perilymph fistula, one of the most controversial and challenging problems for the otologist today. It was determined that the technique is indeed applicable when relatively pure fistula samples are analyzed. Limitations and potential improvements of the technique are discussed. In addition, the potential usefulness of two-dimensional polyacrylamide gel electrophoresis in other pathologic conditions of the inner ear is discussed briefly.

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Effect of Gamma Radiation on Protein Profile, Protein Fraction and Solubility’s of Three Oil Seeds: Soybean, Peanut and Sesame

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha3926252 ◽

2011 ◽

Vol 39 (2) ◽

pp. 90 ◽

Cited By ~ 18

Author(s):

Abd El-Moneim M.R. AFIFY ◽

Mohamed M. RASHED ◽

Ebtesam A. MAHMOUD ◽

Hossam Saad EL-BELTAGI

Keyword(s):

Gamma Radiation ◽

Total Protein ◽

Protein Profile ◽

Polyacrylamide Gel Electrophoresis ◽

Protein Solubility ◽

Γ Irradiation ◽

Small Molecular Weight ◽

Oil Seeds ◽

Complex Protein ◽

Maximum Decrease

The proteins properties of three oil seeds (soybean, peanut and sesame) were investigated following γ-irradiation (0.0, 0.5, 1.0, 2.0, 3.0, 5.0 and 7.5 KGy). The effect of γ-radiation on total protein solubility, albumin, globulin and SDS-ME fractions were studied using SDS-polyacrylamide gel electrophoresis. The results showed that solubility of total protein were decreased and reached to the maximum decrease using irradiation dose of 7.5 KGy compared to control. The interesting phenomena are that albumin and globulin fractions decreased in its solubility while the SDS-ME fraction increased. These phenomena may be due to the effect of gamma radiation on the protein, which may dissociate this fraction to small subunits, and rearrangement to form a complex protein even high or small molecular weight proteins solubilized only in SDS-ME fraction. The changes in protein profile were depended even on radiation dose and on the nature of oil seeds; soybean, peanut and sesame.

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Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples

Molecular Biology International ◽

10.1155/2016/4982131 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Kamal Uddin Zaidi ◽

Abin Mani ◽

Vijay Thawani ◽

Arti Mehra

Keyword(s):

Drug Resistance ◽

Candida Albicans ◽

Amphotericin B ◽

Total Protein ◽

Opportunistic Infections ◽

Protein Profile ◽

Antifungal Susceptibility ◽

Morphological Difference ◽

Clinical Isolates ◽

Clinical Samples

This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

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Antioxidative activity and protein profile of skim milk of Gaddi goats and hill cattle of North West Himalayan region

Veterinary World ◽

10.14202/vetworld.2019.1535-1539 ◽

2019 ◽

Vol 12 (10) ◽

pp. 1535-1539

Author(s):

Vinesh Sharma ◽

Birbal Singh ◽

Rinku Sharma ◽

Jyoti B. Dhar ◽

Neelam Sharma ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Protein ◽

Antioxidative Activity ◽

Protein Profile ◽

Polyacrylamide Gel Electrophoresis ◽

Skim Milk ◽

Total Phenol ◽

Bioactive Metabolites ◽

Crossbred Cattle ◽

Skimmed Milk

Aim: This study was aimed at evaluation of antioxidative activity, protein profile, and vitamins content of milk of Gaddi goats, local non-Gaddi goats, hill cattle, and Jersey crossbred cattle. Materials and Methods: Total phenol, antioxidant activity measured as 2, 2-diphenyl- 1-picrylhydrazyl radical scavenging capacity, total protein, and vitamins were estimated in milk samples by spectrophotometric methods. Milk protein profiles were studied by sulfate-polyacrylamide gel electrophoresis. Results: Total phenol, antioxidant activity, and total protein were higher in indigenous hill cattle skim milk. Average protein content in raw skimmed milk was 1.33±0.01, 1.03±0.02, 0.76±0.05, and 0.81±0.01%, in indigenous hill cattle, Jersey crossbred cattle, non-Gaddi goat, and Gaddi goat, respectively. Three proteins of 19.01, 22.08, and 32.96 kDa were observed in Gaddi goat, but not in non-Gaddi goat skim milk. Furthermore, the above proteins were absent in cattle skim milk. Two proteins of 15.56 and 25.06 kDa were found in local hill and crossbred cattle skimmed milk, but were absent in goat skimmed milk. Vitamin C content was the lowest in Gaddi goat milk and the highest in Jersey crossbred cattle milk. Conclusion: It is envisaged that bioactive metabolites in the milk of Gaddi goats and hill cattle might offer anti-aging and beneficial health effects.

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Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.7862 ◽

2015 ◽

Vol 18 (1) ◽

pp. 58

Author(s):

Sri Darmawati ◽

Langkah Sembiring ◽

Widya Asmara ◽

Wayan T. Artama ◽

Syaiful Anwar

Keyword(s):

Key Words ◽

Total Protein ◽

Reference Strain ◽

Protein Profile ◽

Blood Cultures ◽

Protein Profiles ◽

E Coli ◽

Relationship Analysis ◽

Pair Group ◽

Sds Page

The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%). Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile

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Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000100009 ◽

2013 ◽

Vol 48 (1) ◽

pp. 66-72 ◽

Cited By ~ 7

Author(s):

Karina Freire D'eça Nogueira Santos ◽

Ricardo Diógenes Dias Silveira ◽

Claudia Cristina Garcia Martin-Didonet ◽

Claudio Brondani

Keyword(s):

Amino Acids ◽

Wild Species ◽

Total Protein ◽

Storage Protein ◽

Protein Profile ◽

Protein Fractions ◽

Interspecific Crosses ◽

Major Protein ◽

Rice Cultivars ◽

Oryza Glumaepatula

- The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.

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PERFIL PROTÉICO E EFICIÊNCIA REPRODUTIVA DE VACAS DE CORTE EM LACTAÇÃO SUPLEMENTADAS COM URÉIA NO VERÃO

Archives of Veterinary Science ◽

10.5380/avs.v9i2.4070 ◽

2004 ◽

Vol 9 (2) ◽

Author(s):

R.M. SOUZA ◽

A. FILAPPI ◽

D. PRESTES ◽

M.A. LISTON ◽

A. ANTONIAZZI ◽

...

Keyword(s):

Total Protein ◽

Plasma Levels ◽

Protein Profile ◽

Beef Cows ◽

Pregnancy Rates ◽

Reproductive Efficiency ◽

Reproduction Performance ◽

Group A

O objetivo do presente estudo foi mensurar os níveis plasmáticos de proteína plasmática total (PPT), albumina e uréia, hematócrito e escore da condição corporal (ECC), visando avaliar o efeito da suplementação com uréia sobre o perfil protéico e o índice de prenhez (IP) de vacas de corte em lactação, mantidas em campo nativo, durante o período de verão. O experimento ocorreu entre setembro/2002 (primavera) e fevereiro/2003 (verão). As matrizes foram divididas aleatoriamente em dois grupos: controle © n=161) e tratado (T) n=159). Os grupos receberam mistura mineral, porém o T, recebeu adicionalmente 10% de uréia agrícola em janeiro e fevereiro. Relativamente a PPT, não foi observada diferença entre T e C, no verão. Na albumina, não foi observada diferença entre primavera e verão. O C apresentou concentrações maiores no verão do que o T. Os valores do hematócrito, no C de verão foram maiores do que os do C da primavera e T de verão. Na uréia o grupo T, no verão, apresentou níveis maiores do que os C de primavera e verão. Não houve diferença nos ECC entre primavera e verão, assim como entre T e C, no verão. Quanto ao IP, não houve diferença entre C e T. Em conclusão, a uréia tem efeitos deletérios sobre o perfil protéico de vacas de corte com cria ao pé, no verão, quando há alta disponibilidade de massa verde. A adição de uréia à mistura mineral não influência a eficiência reprodutiva das matrizes. Protein profile and reproductive efficiency of lactating beef cows, supplemented with urea during the summer Abstract The objective of the present study was to assay total protein (PPT), albumin and urea plasma levels, hematocrit, body conditions score (BCS) and pregnancy rates (PR) in beef cows supplemented or not with urea during the summer. The experiment started in September (spring) 2002 and ended in February (summer) 2003. The dams were randomly assigned in two experimental groups: control (C; n = 161) and treated (T; n = 159). Both groups received a complete mineral mix. For the T group, a total of 10% of agricultural grade urea was added to the mix during January and February. As a result, no differences in PPT has been observed between both experimental groups during the summer. However, in regard to plasmatic albumin no differences were found for both experimental groups between spring and summer but values showed to be higher for the C group than in T group during summer. There were no differences in BCS nor in IP. The addition of urea to the mineral mix has deleterious effects upon the protein profile os suckling beef cows, when trere is high avalability of forage. The use of urea has no effect on reproduction performance.

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Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Reproduction ◽

10.1530/rep.1.00581 ◽

2006 ◽

Vol 131 (3) ◽

pp. 449-460 ◽

Cited By ~ 39

Author(s):

A A Fouladi-Nashta ◽

K H S Campbell

Keyword(s):

Oocyte Maturation ◽

Total Protein ◽

Culture Medium ◽

Protein Pattern ◽

Protein Profile ◽

Cell Number ◽

Blastocyst Stage ◽

Cleavage Rate ◽

Developmental Potential ◽

Follicle Culture

Follicles of 4–8 mm in diameter were dissected from ovaries and cultured in Waymouth culture medium in the presence or absence of insulin (5 μg/ml) at 39 °C in a humidified atmosphere of 45% O2, 5% CO2and 50% N2for 24 h. Following follicle culture, the oocytes were collected and examined for developmental potential, total protein profile and ultrastructural aspects. Oocytes aspirated directly from follicles of the same size were used as controls. Addition of insulin to the follicle culture medium significantly reduced expression of the low molecular weight insulin-like growth factor-binding proteins (IGFBPs) in the follicular fluid, and significantly reduced the cleavage rate of subsequently matured and fertilised oocytes (0.52 vs 0.61). However, there were no differences in the proportion of cleaved embryos which developed to the blastocyst stage (0.30 vs 0.28), nor embryo quality as assessed by total cell number (137 ± 8.53 vs124.6 ± 6.95). The total protein profiles of immature oocytes recovered after 24 h of follicle culture were compared by PAGE. There were marked differences between the two groups, unmatured oocytes recovered from the insulin-positive follicle group showed a protein pattern similar to that of matured oocytes. In addition, examination of ultrastructural features by transmission electron microscopy indicated that oocytes from follicles cultured in the presence of insulin undergo many of the cytoplasmic changes associated with oocyte maturation. In conclusion, follicle culture in the presence of insulin is beneficial for follicular survival and significantly reduces cleavage but has no detrimental effects on the development of cultured embryos. However, many of the cytoplasmic changes associated with oocyte maturation occur prior to the induction of nuclear maturation.

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