Utilizing hydrolysis and acidification via liquid fraction of digestate (LFD-HA) for methane production enhancement of corn straw: Physicochemical and microbial community characterization

2021 ◽  
pp. 129282
Author(s):  
Yue Liu ◽  
Hui Gong ◽  
Shuting He ◽  
Chuan Shi ◽  
Hairong Yuan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Liquid Fraction ◽  
Corn Straw

scholarly journals Effect of Biochar Addition on the Microbial Community and Methane Production in the Rapid Degradation Process of Corn Straw

Energies ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2223
Author(s):  
Zhi Wang ◽  
Ying Guo ◽  
Weiwei Wang ◽  
Liumeng Chen ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Biogas Production ◽  
Degradation Process ◽  
Hydrolysis Rate ◽  
Control Group ◽  
Corn Straw ◽  
High Concentration ◽  
Fermentation Time

Anaerobic digestion with corn straw faces the problems of difficult degradation, long fermentation time and acid accumulation in the high concentration of feedstocks. In order to speed up the process of methane production, corn straw treated with sodium hydroxide was used in thermophilic (50 °C) anaerobic digestion, and the effects of biochar addition on the performance of methane production and the microbial community were analyzed. The results showed that the cumulative methane production of all treatment groups reached over 75% of the theoretical methane yield in 7 days and the addition of 4% biochar increased the cumulative methane production by 6.75% compared to the control group. The addition of biochar also decreased the number of biogas and methane production peaks from 2 to 1, and had a positive effect on shortening the digestion start-up period and reducing the fluctuation of biogas production during the digestion process. The addition of 4% biochar increased the abundance of the bacterial family Peptococcaceae throughout the digestion period, promoting the hydrolysis rate of corn straw. The dominant archaeal genus Methanosarcina was significantly more abundant at the peak stage and the end of methane production with 4% biochar added compared to the control group.

Anaerobic co-digestion of organic fractions of municipal solid waste: Synergy study of methane production and microbial community

2021 ◽  
Vol 151 ◽  
pp. 106137
Author(s):  
Yiran Zhou ◽  
Kangyi Huang ◽  
Xiuyao Jiao ◽  
Nemanja Stanisavljevic ◽  
Lei Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Municipal Solid Waste ◽  
Solid Waste ◽  
Methane Production ◽  
Organic Fractions

scholarly journals Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

2017 ◽  
Vol 43 (3) ◽  
pp. 53-60 ◽  
Author(s):  
Piotr Świątczak ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Paulina Rusanowska
Keyword(s):  
Microbial Community ◽  
Sewage Sludge ◽  
Methane Production ◽  
Treatment Plant ◽  
Full Scale ◽  
Successful Implementation ◽  
Anaerobic Digesters ◽  
Syntrophic Bacteria ◽  
The Stability ◽  
Microbial Groups

AbstractAnaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34–35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m3/d, for a sludge load of 0.054 m3/(m3·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) wereMethanosaetaandMethanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the orderBacteroidetes, including the classCytophagia(11.6% of all sequences), indicated the high potential of the biomass for efficient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.

scholarly journals Microbial Community Analysis Using MiSeq Sequencing and Pathway of Methane Production in Tehran WWTP: A Full-Scale Anaerobic Digester

2020 ◽  
Author(s):  
Reza Barati Rashvanlou ◽  
Mahdi Farzadkia ◽  
Abbas Ali Moserzadeh ◽  
Asghar Riazati ◽  
Chiang Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Community Analysis ◽  
Full Scale ◽  
Microbial Community Analysis ◽  
Miseq Sequencing ◽  
Sequencing Method ◽  
Community Data ◽  
Rank Abundance ◽  
Made In

Introduction: One of biological wastewater treatment methods that utilizes to both digesting waste activated sludge and methane production is anaerobic digestion (AD). It is believed to be most effective solution in terms of energy crisis and environmental pollution issues. Materials and Methods: In this study the sludge was digested anaerobically sampled from a full-scale WWTP, located at south of Tehran, Iran for evaluation. To study the microbial community within the sludge the MiSeq Sequencing method utilized. Based on our field data (data not shown) and microbial community data, a schematic diagram of probable leading pathways was made in the studied digester. Results: At first, the community variety in the bulk sludge and richness were enhanced followed by loading increasing. Meanwhile, the loading change enhanced the community richness and variety of the sludge. By comparing the rank-abundance distributions, a shallow gradient would show high evenness since the abundances of diverse species are alike. The results showed all the communities were extremely diverse and 15 phyla were distinguished in the sludge sample. The dominant phyla of the community were Bacteroidetes and Firmicutes and quantity of the two phyla were 21% and 11%, respectively. Anaerobaculum, Acinetobacter, Syntrophomonas, and Coprothermobacter were the chief genera for the microbial communities and the sum of four genera were 7%, 3%, 3%, and 2%, respectively. Conclusion: It was shown that syntrophic acetate oxidizing bacterias (SAOBs) metabolized acetate through hydrogen trophic methanogenesis in the digester. Generally, the findings may be useful to help the wastewater operators to utilize an effective method that able to treat waste sludge plus methane production, simultaneously.

scholarly journals Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Jingjing Wan ◽  
Yuhang Jing ◽  
Yue Rao ◽  
Shicheng Zhang ◽  
Gang Luo
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Quantitative Pcr ◽  
Methane Production ◽  
Bacterial Pathogens ◽  
Methane Yield ◽  
Metagenomic Analysis ◽  
Waste Activated Sludge ◽  
Hydrogen Yield

ABSTRACT Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H 2 /g volatile solids [VS]) and methane yield (150.7 ml CH 4 /g VS) in the TM process were higher than those (6.7 ml H 2 /g VS and 127.8 ml CH 4 /g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH 4 /g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that different microbial community compositions were established in the hydrogen reactors of the TM and MM processes, which also significantly changed the microbial community compositions in the following methane reactors compared to that with the M process. The dynamics of bacterial pathogens were also evaluated. For the TM process, the reduced diversity and total abundance of bacterial pathogens in WAS were observed in the hydrogen reactor and were further reduced in the methane reactor, as revealed by metagenomic analysis. The results also showed not all bacterial pathogens were reduced in the reactors. For example, Collinsella aerofaciens was enriched in the hydrogen reactor, which was also confirmed by quantitative PCR (qPCR) analysis. The study further showed that qPCR was more sensitive for detecting bacterial pathogens than metagenomic analysis. Although there were some differences in the relative abundances of bacterial pathogens calculated by metagenomic and qPCR approaches, both approaches demonstrated that the TM process was more efficient for the removal of bacterial pathogens than the MM and M processes. IMPORTANCE This study developed an efficient process for bioenergy (H 2 and CH 4 ) production from WAS and elucidates the dynamics of bacterial pathogens in the process, which is important for the utilization and safe application of WAS. The study also made an attempt to combine metagenomic and qPCR analyses to reveal the dynamics of bacterial pathogens in anaerobic processes, which could overcome the limitations of each method and provide new insights regarding bacterial pathogens in environmental samples.

scholarly journals Co-digestion of microalgae with potato processing waste and glycerol: effect of glycerol addition on methane production and the microbial community

RSC Advances ◽  
2020 ◽  
Vol 10 (61) ◽  
pp. 37391-37408
Author(s):  
Yanghanzi Zhang ◽  
Gary S. Caldwell ◽  
Philip T. Blythe ◽  
Andrew M. Zealand ◽  
Shuo Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Processing Waste ◽  
Potato Processing ◽  
Potato Waste

Glycerol as an additional co-substrate enhanced methane yields by up to 128% when co-digestion with microalgae and potato waste.

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