Sedative-hypnotic, anxiolytic and possible side effects of Salvia limbata C. A. Mey. Extracts and the effects of phenological stage and altitude on the rosmarinic acid content

Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surface methodology (RSM) was performed to develop an extraction method with maximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21 min, ultrasound amplitudes 62 A, and solvent composition 70% ethanol in water. The crude extract was further fractionated using solid-phase extraction (SPE), where six sequential fractions that varied in polarity (0–100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.

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Antioxidant activity, total phenolics, and rosmarinic acid content in different basils (Ocimum spp.)

Israel Journal of Plant Sciences ◽

10.1560/ijps.58.3-4.191 ◽

2010 ◽

Vol 58 (3) ◽

pp. 191-195 ◽

Cited By ~ 6

Author(s):

Adolfina R. Koroch ◽

Hector R. Juliani ◽

Cedric Sims ◽

James E. Simon

Keyword(s):

Antioxidant Activity ◽

Acid Content ◽

Rosmarinic Acid ◽

Total Phenolics

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Antioxidative / Acetylcholinesterase Inhibitory Activity of Some Asteraceae Plants

Natural Product Communications ◽

10.1177/1934578x1300800416 ◽

2013 ◽

Vol 8 (4) ◽

pp. 1934578X1300800

Author(s):

Ivana Generalić Mekinić ◽

Franko Burčul ◽

Ivica Blaževieć ◽

Danijela Skroza ◽

Daniela Kerum ◽

...

Keyword(s):

Inhibitory Activity ◽

Acid Content ◽

Rosmarinic Acid ◽

Total Phenolic ◽

Artemisia Absinthium ◽

Arctium Lappa ◽

Spectrophotometric Methods ◽

Acetylcholinesterase Inhibitory Activity ◽

Linear Relationships ◽

Inula Helenium

The extracts obtained by 80% EtOH from some Asteraceae plants ( Calendula officinalis, Inula helenium, Arctium lappa, Artemisia absinthium and Achillea millefolium) were studied. Rosmarinic acid, one of the main compounds identified in all extracts, was determined quantitatively by using HPLC. In addition, spectrophotometric methods were evaluated as an alternative for rosmarinic acid content determination. Total phenolic content was also established for all extracts. A. millefolium extract was found to have the highest content of rosmarinic acid as well as total phenols. All extracts were tested for antioxidant and acetylcholinesterase inhibitory activity. A. millefolium was shown to possess the best antioxidant activity (for all tested methods) as well as acetylcholinesterase inhibitory activity. Highly positive linear relationships were obtained between antioxidant/acetylcholinesterase inhibitory activity and the determined rosmarinic acid content indicating its significance for the observed activities.

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ANTIOXIDANT PROPERTIES OFO. ONITES,T. VULGARISANDO. BASILICUMSPECIES GROWN IN GREECE AND THEIR TOTAL PHENOL AND ROSMARINIC ACID CONTENT

Journal of Food Lipids ◽

10.1111/j.1745-4522.2009.01161.x ◽

2009 ◽

Vol 16 (4) ◽

pp. 484-498 ◽

Cited By ~ 11

Author(s):

V. LAGOURI ◽

E. NISTEROPOULOU

Keyword(s):

Acid Content ◽

Rosmarinic Acid ◽

Antioxidant Properties ◽

Total Phenol

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Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Acta agriculturae Slovenica ◽

10.14720/aas.2016.107.1.05 ◽

2016 ◽

Vol 107 (1) ◽

pp. 45 ◽

Cited By ~ 8

Author(s):

Elnaz NOUROZI ◽

Bahman HOSSEINI ◽

Abbas HASSANI

Keyword(s):

Growth Rate ◽

Hairy Root ◽

Hairy Roots ◽

Acid Content ◽

Rosmarinic Acid ◽

Culture Media ◽

Root Induction ◽

Transformed Roots ◽

Inoculation Methods ◽

Hairy Root Induction

Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A. rhizogenes, rosmarinic acid content and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of A. foeniculum was established by inoculation of the 1-month-old leaf explant with A4 strain of A. rhizogenes and the effectiveness of light – dark conditions and two inoculation methods (immersion and injection) were tested. Furthermore, in immersion method, the effects of inoculation time (3, 5 and 7 min) on root induction were investigated. In the second part of the study, the hairy root culture of A. foeniculum was studied using different basal culture media (MS, 1/2 MS and B5). Rosmarinic acid content in hairy roots and non- transformed roots was analyzed using high-performance liquid chromatography (HPLC). There was no significant difference between various inoculation methods in the ability of hairy roots induction. Observations showed that percentage of hairy root induction was higher when the explants were immersed for 5 min in bacterial suspension. Light conditions displayed the highest hairy root induction rates compared with dark condition. Various culture media are different in terms of types and amounts of nutrients and have influence on growth rate. The maximum growth rate (1.61 g fr wt/50 ml) of hairy roots were obtained in 1/2 MS medium. Rosmarinic acid content in transformed roots (213.42 µg/g dry wt) was significantly higher than non-transformed roots (52.28 µg/ g dry wt).

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