scholarly journals Puerarin improves intestinal barrier function through enhancing goblet cells and mucus barrier

2020 ◽  
Vol 75 ◽  
pp. 104246
Author(s):  
Jiaojiao Li ◽  
Li Zhang ◽  
Yafei Li ◽  
Yi Wu ◽  
Tao Wu ◽  
...  
Keyword(s):  
Barrier Function ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Intestinal Barrier Function ◽  
Mucus Barrier

scholarly journals 138 Effects of Diet Complexity and L-Threonine Supplementation on Immune Response and Intestinal Barrier Function in Nursery Pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 99-99
Author(s):  
Bonjin Koo ◽  
Janghan Choi ◽  
Chengbo Yang ◽  
Charles Martin Nyachoti
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Proximal Colon ◽  
Intestinal Barrier Function ◽  
Experimental Unit ◽  
Animal Products ◽  
Nursery Pigs

Abstract The aim was to investigate the effects of diet complexity and L-Thr supplementation on immune response and intestinal barrier function in nursery pigs. Thirty-two pigs (7.23 ± 0.48 kg of body weight) were randomly assigned dietary treatments in a 2 × 2 factorial arrangement based on diet complexity (complex or simple) and L-Thr supplementation (standard, NRC 2012 or 15% more). The complex diet contained fish meal, plasma protein and dried whey to mimic a conventional nursery diet. The simple diet was formulated with corn, wheat, and soybean meal and did not contain any animal products. Pigs were individually housed and fed experimental diets ad libitum for 14 d. All data were analyzed using mixed procedure of SAS with the individual pen as the experimental unit. Pigs fed the simple diet had greater (P < 0.05) plasma interleukin (IL)-6 and IL-10 concentrations compared to those fed the complex diet. Pigs fed the simple diet tended to show greater (P < 0.10) expression of genes encoding for tumor necrosis factor-α, claudin-1, and zonula occludens-1 in the jejunum compared to those fed the complex diet. The addition of L-Thr increased (P < 0.05) villus height and numbers of both villi and crypt goblet cells in the jejunum and deepened (P < 0.05) crypts in the proximal colon. Also, L-Thr-supplemented diets upregulated (P < 0.05) the expression of the gene encoding for occludin and tended to downregulate (P < 0.10) IL-6 gene expression in the jejunum. Trends (P < 0.10) for interaction between diet complexity and L-Thr supplementation were observed in villus height:crypt depth ratio, the number of goblet cells, and IL-6 gene expression in the jejunum. In conclusion, feeding a simple diet stimulated the immune system of nursery pigs compared to a complex diet. Dietary L-Thr supplementation fortified intestinal structure and function.

scholarly journals Lactic Acid Bacteria May Impact Intestinal Barrier Function by Modulating Goblet Cells

2018 ◽  
Vol 62 (6) ◽  
pp. 1700572 ◽  
Author(s):  
Chengcheng Ren ◽  
Jelleke Dokter-Fokkens ◽  
Susana Figueroa Lozano ◽  
Qiuxiang Zhang ◽  
Bart J. de Haan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Intestinal Barrier Function

Dietary squid ink polysaccharide induces goblet cells to protect small intestine from chemotherapy induced injury

2015 ◽  
Vol 6 (3) ◽  
pp. 981-986 ◽  
Author(s):  
Tao Zuo ◽  
Lu Cao ◽  
Changhu Xue ◽  
Qing-Juan Tang
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Intestinal Barrier Function ◽  
Cancer Drugs ◽  
Anti Cancer ◽  
Gastrointestinal Mucositis ◽  
Potential Damage

Gastrointestinal mucositis induced by chemotherapy is associated with alterations of intestinal barrier function due to the potential damage induced by anti-cancer drugs on the epithelial cells.

scholarly journals Association of Heterophil/Lymphocyte Ratio with Intestinal Barrier Function and Immune Response to Salmonella enteritidis Infection in Chicken

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3498
Author(s):  
Mamadou Thiam ◽  
Astrid Lissette Barreto Sánchez ◽  
Jin Zhang ◽  
Maiqing Zheng ◽  
Jie Wen ◽  
...  
Keyword(s):  
Immune Response ◽  
Barrier Function ◽  
Salmonella Enteritidis ◽  
Current Knowledge ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Intestinal Barrier Function ◽  
Immune Gene ◽  
Intestinal Immunity ◽  
Ileal Mucosa

The heterophil/lymphocyte (H/L) ratio has been extensively studied to select poultry that are resistant to environmental stressors. Chickens with a low H/L ratio are superior to the chickens with a high H/L ratio in survival, immune response, and resistance to Salmonella infection. However, this disease resistance ability is likely to be associated with enhanced intestinal immunity. Therefore, to expand our understanding of these underlying resistance mechanisms, it is crucial to investigate the correlation between the H/L ratio as a blood immune indicator in live chickens and the intestinal barrier function and immunity. Jinxing yellow chickens H/L line one-day-old were divided into non-infected (NI) and Salmonella enteritidis infected (SI) at 7-days old. After dividing the birds into NI and SI, blood samples were taken for H/L ratios determination, and subsequently, birds from the SI group were infected with Salmonella enteritidis (SE). We assessed the effects of SE infection on the (i) goblet cells number from the ileum and caecum gut-segments, (ii) ileal mucosa morphology, and (iii) immune gene mRNA expressions from the ileum and caecum of NI and SI chickens at 7 and 21 days-post-infection (dpi). We found that the H/L ratio was negatively correlated with most intestinal immune indices, particularly with the goblet cells number and with IL-1β, IL-8, and IFN-γ ileal expressions. In conclusion, these results suggest that the H/L ratio is associated with the intestinal barrier and immune response for SE clearance and that the chickens with a low H/L ratio displayed enhanced intestinal immunity. This study expands the current knowledge that is related to using the H/L ratio to select and breed resistant broiler chickens.

Galacto-oligosaccharides may directly enhance intestinal barrier function through the modulation of goblet cells

2014 ◽  
Vol 59 (3) ◽  
pp. 566-573 ◽  
Author(s):  
Shikha Bhatia ◽  
P. Nagendra Prabhu ◽  
Ann C. Benefiel ◽  
Michael J. Miller ◽  
JoMay Chow ◽  
...  
Keyword(s):  
Barrier Function ◽  
Intestinal Barrier ◽  
Goblet Cells ◽  
Intestinal Barrier Function

Influence of Plant Bioactive Compounds on Intestinal Epithelial Barrier in Poultry

2020 ◽  
Vol 20 (7) ◽  
pp. 566-577 ◽  
Author(s):  
Amlan Kumar Patra
Keyword(s):  
Bioactive Compounds ◽  
Barrier Function ◽  
Molecular Mechanisms ◽  
Digestive Enzyme ◽  
Intestinal Barrier ◽  
Feed Additives ◽  
Interactive Effects ◽  
Intestinal Barrier Function ◽  
Poultry Production ◽  
Poultry Feeding

Natural plant bioactive compounds (PBC) have recently been explored as feed additives to improve productivity, health and welfare of poultry following ban or restriction of in-feed antibiotic use. Depending upon the types of PBC, they possess antimicrobial, digestive enzyme secretion stimulation, antioxidant and many pharmacological properties, which are responsible for beneficial effects in poultry production. Moreover, they may also improve the intestinal barrier function and nutrient transport. In this review, the effects of different PBC on the barrier function, permeability of intestinal epithelia and their mechanism of actions are discussed, focusing on poultry feeding. Dietary PBC may regulate intestinal barrier function through several molecular mechanisms by interacting with different metabolic cascades and cellular transcription signals, which may then modulate expressions of genes and their proteins in the tight junction (e.g., claudins, occludin and junctional adhesion molecules), adherens junction (e.g., E-cadherin), other intercellular junctional proteins (e.g., zonula occludens and catenins), and regulatory proteins (e.g., kinases). Interactive effects of PBC on immunomodulation via expressions of several cytokines, chemokines, complement components, pattern recognition receptors and their transcription factors and cellular immune system, and alteration of mucin gene expressions and goblet cell abundances in the intestine may change barrier functions. The effects of PBC are not consistent among the studies depending upon the type and dose of PBC, physiological conditions and parts of the intestine in chickens. An effective concentration in diets and specific molecular mechanisms of PBC need to be elucidated to understand intestinal barrier functionality in a better way in poultry feeding.

scholarly journals BAFF Blockade Attenuates Inflammatory Responses and Intestinal Barrier Dysfunction in a Murine Endotoxemia Model

2020 ◽  
Vol 11 ◽  
Author(s):  
Runze Quan ◽  
Chaoyue Chen ◽  
Wei Yan ◽  
Ying Zhang ◽  
Xi Zhao ◽  
...  
Keyword(s):  
Barrier Function ◽  
Intestinal Inflammation ◽  
Inflammatory Responses ◽  
Survival Rates ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Barrier Dysfunction ◽  
Protein Levels ◽  
Lps Challenge ◽  
Endotoxemia Model

B cell-activating factor (BAFF) production is increased in septic patients. However, the specific role of BAFF in sepsis remains unknown. This study was designed to investigate the expression and function of BAFF in an experimental endotoxemia model and to identify the potential mechanisms. We established an endotoxemia mouse (6–8 weeks, 20–22 g) model by administering 30 mg/kg lipopolysaccharide (LPS). BAFF levels in the circulating system and organ tissues were measured 4 and 8 h after LPS injection. Survival rates in the endotoxemia mice were monitored for 72 h after BAFF blockade. The effects of BAFF blockade on systemic and local inflammation, organ injuries, and intestinal barrier function were also evaluated 4 h after LPS treatment. BAFF production was systemically and locally elevated after LPS challenge. BAFF blockade improved the survival rate, systemic inflammation, and multi-organ injuries. Moreover, BAFF blockade attenuated both intestinal inflammation and impaired intestinal permeability. BAFF blockade upregulated ZO-1 and occludin protein levels via the NF-κB/MLCK/MLC signaling pathway. These results suggested that BAFF blockade protects against lethal endotoxemia at least partially by alleviating inflammation, multi-organ injuries, and improving intestinal barrier function and provides a novel focus for further research on sepsis and experimental evidence for clinical therapy.

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