Enhanced permeability and photoprotective potential of optimized p-coumaric acid-phospholipid complex loaded gel against UVA mediated oxidative stress

To protect against oxidative stress-induced apoptosis in lens epithelial cells is a potential strategy in preventing cataract formation. The present study aimed at studying the protective effect and underlying mechanisms of p-coumaric acid (p-CA) on hydrogen peroxide- (H2O2-) induced apoptosis in human lens epithelial (HLE) cells (SRA 01–04). Cells were pretreated with p-CA at a concentration of 3, 10, and 30 μM before the treatment of H2O2 (275 μM). Results showed that pretreatment with p-CA significantly protected against H2O2-induced cell death in a dose-dependent manner, as well as downregulating the expressions of both cleaved caspase-3 and cleaved caspase-9 in HLE cells. Moreover, p-CA also greatly suppressed H2O2-induced intracellular ROS production and mitochondrial membrane potential loss and elevated the activities of T-SOD, CAT, and GSH-Px of H2O2-treated cells. As well, in vitro study showed that p-CA also suppressed H2O2-induced phosphorylation of p-38, ERK, and JNK in HLE cells. These findings demonstrate that p-CA suppresses H2O2-induced HLE cell apoptosis through modulating MAPK signaling pathways and suggest that p-CA has a potential therapeutic role in the prevention of cataract.

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Yak-Kong Soybean (Glycine max) Fermented by a Novel Pediococcus pentosaceus Inhibits the Oxidative Stress-Induced Monocyte–Endothelial Cell Adhesion

Nutrients ◽

10.3390/nu11061380 ◽

2019 ◽

Vol 11 (6) ◽

pp. 1380 ◽

Cited By ~ 1

Author(s):

Ji Seung Kim ◽

Jong Hun Kim ◽

Sasikumar Arunachalam Palaniyandi ◽

Charles C. Lee ◽

Ji Woo You ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Adhesion ◽

Glycine Max ◽

Endothelial Cell ◽

Phenolic Acids ◽

Coumaric Acid ◽

Antioxidant Ability ◽

Pediococcus Pentosaceus ◽

Black Soybean ◽

Endothelial Cell Adhesion

Yak-Kong (YK), a small black soybean (Glycine max) in Korea, contained higher concentrations of antioxidants than ordinary black soybean or yellow soybean in our previous study. We prepared the fermented YK extract by using a novel lactic acid bacterium, Pediococcus pentosaceus AOA2017 (AOA2017) isolated from Eleusine coracana, and found that the antioxidant ability was enhanced after fermentation. In order to investigate the cause of the enhanced antioxidant ability in the fermented YK extract, we conducted a phenolic composition analysis. The results show that proanthocyanidin decreased and phenolic acids increased with a statistical significance after fermentation. Among the phenolic acids, p-coumaric acid was newly produced at about 11.7 mg/100 g, which did not exist before the fermentation. Further, the fermented YK extract with increased p-coumaric acid significantly inhibited the lipopolysaccharide-induced THP-1 monocyte–endothelial cell adhesion compared to the unfermented YK extract. The fermented YK extract also suppressed the protein expression levels of vascular cell adhesion molecule (VCAM)-1 in human umbilical vein endothelial cells (HUVECs). Together with the previous studies, our results suggest that the extract of YK fermented by AOA2017 has potential to be a new functional food material with its enhanced bioactive compounds which may help to prevent atherosclerosis caused by oxidative stress.

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P-Coumaric Acid Mitigates Doxorubicin-Induced Nephrotoxicity Through Suppression of Oxidative Stress, Inflammation and Apoptosis

Archives of Medical Research ◽

10.1016/j.arcmed.2019.12.004 ◽

2020 ◽

Vol 51 (1) ◽

pp. 32-40 ◽

Cited By ~ 5

Author(s):

Zeinab Rafiee ◽

Maasoumeh Zare Moaiedi ◽

Armita Valizade Gorji ◽

Esrafil Mansouri

Keyword(s):

Oxidative Stress ◽

Coumaric Acid

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Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480

Frontiers in Microbiology ◽

10.3389/fmicb.2021.747868 ◽

2021 ◽

Vol 12 ◽

Author(s):

Daniela Catrileo ◽

Sandra Moreira ◽

María Angélica Ganga ◽

Liliana Godoy

Keyword(s):

Oxidative Stress ◽

Antimicrobial Properties ◽

Growth Curves ◽

Wine Industry ◽

Economic Losses ◽

Protective Effects ◽

Coumaric Acid ◽

Volatile Phenols ◽

Brettanomyces Bruxellensis ◽

Expression Of Genes

Brettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.

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HPLC Profile of Phenolic Contents, Antioxidant and Antidiabetic Activities of Methanolic Extract of the Leaves of Sarcocephalus latifolius(Bruce, Smith) Grown in North Central Geopolitical Zone, Nigeria

Jordan Journal of Chemistry ◽

10.47014/15.3.1 ◽

2020 ◽

Vol 15 (3) ◽

pp. 103-110

Keyword(s):

Oxidative Stress ◽

Leaf Extract ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Coumaric Acid ◽

Phytochemical Screening ◽

North Central ◽

Butanol Fraction ◽

Phenolic Contents ◽

Hplc Profile

Abstract: Preliminary phytochemical screening of the n-butanol fraction of the methanol leaf extract of Sarcocephalus latifoliuswas carried out;its phenolic contents were quantified by HPLC. The in vitroantihyperglycemic and antioxidant activities of the fraction were evaluated.The results of the present study indicated that the leaves’extract contained phytochemicals,like phenols, flavonoids, alkaloids and glycosides.Phenolic compounds,such as garlic acid, catechin, ferrulic acid, p-coumaric acid, apigen-7-glucose, kaemferol, quercetin and amentoflavone,were quantified by HPLC. Moreover, the fraction of leaves’extract of S.latifoliusdemonstrated significant antioxidant and antihyperglyce-mic activities.The presence of these phytochemicals,especially phenols and flavonoids,in the leavesof S.latifoliustherefore justifiesitsmedicinal usefulness,as this plant has the potential to protect oxidative stress-induced ailments

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Brazilian green propolis promotes the cytoprotective expression of heme oxygenase-1 against oxidative stress injury in murine myoblast cells

Functional Foods in Health and Disease ◽

10.31989/ffhd.v10i12.756 ◽

2020 ◽

Vol 10 (12) ◽

pp. 493

Author(s):

Wataru Otsu ◽

Naoki Chinen ◽

Kazuki Ohuchi ◽

Shiori Ando ◽

Shinsuke Nakamura ◽

...

Keyword(s):

Oxidative Stress ◽

Caffeic Acid ◽

Heme Oxygenase ◽

Heme Oxygenase 1 ◽

Oxygen Species ◽

Coumaric Acid ◽

Stress Injury ◽

Antioxidative Effects ◽

Myoblast Cell ◽

Myoblast Cells

Background: Sarcopenia is a progressive skeletal muscle disorder characterized by the progressive loss of muscle mass and function, resulting in physical disability and mortality. Although sarcopenia impacts a large proportion of elderly individuals, no effective treatment for this disease has yet been identified. The excessive production of reactive oxygen species (ROS) can damage tissues and promote aging, and the daily use of dietary antioxidants can be effective for maintaining skeletal muscle health. Propolis, a natural substance that is collected by honey bees, has been used as traditional medicine, and many reports have described its antioxidative properties. However, how propolis exhibits cytoprotective effects and antioxidative effects in skeletal muscles remains unclear. The purpose of this study was to investigate the antioxidative effects of ethanol-extracted Brazilian green propolis (EEBP, from Baccharis dracunculifolia) and its three constituents using an in vitro myoblast cell model.Methods: Murine myoblast C2C12 cells were treated with either EEBP or its constituents, including caffeic acid, trans-ferulic acid, and p-coumaric acid, in the presence of 100 or 300 mM H2O2 to induce oxidative stress injury. The cell death ratio and cell viability were assessed by Hoechst 33342 and propidium iodide staining and the WST-8 assay, respectively. Simultaneously, intracellular ROS production was measured by CM-H2DCFDA [5-(and-6)-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester] assay. Finally, immunoblotting was performed in myoblast cell lysates to assess the expression level of an antioxidative enzyme, heme oxygenase-1 (HO-1).Results: We demonstrated that EEBP significantly reduced H2O2-induced cell death at a concentration of 3 µg/ml in myoblasts. Additionally, caffeic acid at 100 µM improved cell viability under oxidative stress conditions, but not trans-ferulic acid or p-coumaric acid. Both EEBP and caffeic acid inhibited the H2O2-induced increase in ROS production. Finally, HO-1 expression was increased by treatment with either EEBP or caffeic acid. The increase in HO-1 expression induced by H2O2 was enhanced in the presence of EEBP and caffeic acid.Conclusions: These findings indicated that EEBP has protective effects against oxidative damage in C2C12 murine myoblast cell line. Caffeic acid is an EEBP constituent that contributes to cytoprotective activity. EEBP may act as an inducer of HO-1 to prevent oxidative stress-induced myoblast death.Keywords: C2C12 murine myoblast cells, heme oxygenase-1, oxidative stress, propolis, reactive oxygen species

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p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis

Life Sciences ◽

10.1016/j.lfs.2021.119546 ◽

2021 ◽

Vol 278 ◽

pp. 119546

Author(s):

Yun-Hong Li ◽

Qian He ◽

Yu-Zhong Chen ◽

Ya-Fang Du ◽

Ya-Xin Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Ionizing Radiation ◽

Intestinal Injury ◽

Coumaric Acid ◽

Radiation Induced

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AMELIORATIVE EFFECTS OF GALLIC ACID AND P-COUMARIC ACID ON OXIDATIVE STRESS AND HAEMATOLOGICAL ABNORMALITIES IN DIABETIC RATS

Egyptian Journal of Zoology ◽

10.12816/0049662 ◽

2018 ◽

Vol 69 (69) ◽

pp. 55-72

Author(s):

Eman Abdel Reheim ◽

Adel Abdel-Moneim ◽

Sanaa Abd El-Twab ◽

Mohamed Ashour ◽

Ahmed Yousef

Keyword(s):

Oxidative Stress ◽

Gallic Acid ◽

Diabetic Rats ◽

Coumaric Acid

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Contribution and Interactions of Hydroxycinnamic Acids Found in Bran and Wholegrain Sorghum (Sorghum bicolorL. Moench): Effects on the Antioxidant Capacity and Inhibition of Human Erythrocyte Hemolysis

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/8219023 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Norma Julieta Salazar-López ◽

Gustavo A. González-Aguilar ◽

Guadalupe Loarca-Piña ◽

Francisco J. Cinco-Moroyoqui ◽

Ofelia Rouzaud-Sández ◽

...

Keyword(s):

Oxidative Stress ◽

Ferulic Acid ◽

Antioxidant Capacity ◽

Functional Foods ◽

Hydroxycinnamic Acids ◽

Cellular Aging ◽

Noncommunicable Diseases ◽

Erythrocyte Membranes ◽

Coumaric Acid ◽

Whole Grain

An imbalance between free radicals and antioxidants is known as oxidative stress, and it promotes cellular aging and the development of chronic noncommunicable diseases. The bioactive compounds present in food play an important role in preventing oxidative stress. The aim of this study was to determine the contributions and interactions of the hydroxycinnamic acids found in the bran and whole grain of sorghum and to evaluate their effects on the antioxidant capacity and inhibition of the hemolysis of human erythrocytes. Results showed that the caffeic acid,p-coumaric acid, and ferulic acid found in sorghum contributed to the scavenging of DPPH and ABTS radicals in various proportions. Ferulic acid, which was present in bound form in the bran and wholegrain sorghum, significantly inhibited the AAPH radical-induced oxidation of the erythrocyte membranes by 78.0 and 4.3%, respectively. Combinations of two, three, or four hydroxycinnamic acids may interact in an antagonistic or synergistic manner, thereby altering each other’s bioactivities. The various interactions between the different sorghum bioactives can have a significant impact on their potential bioactivities. These results can be useful in the design of functional foods that aim to deliver bioactives to mitigate cellular aging or noncommunicable diseases.

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