Mitochondrial activity and oxidative stress markers in peripheral blood mononuclear cells of patients with bipolar disorder, schizophrenia, and healthy subjects

2013 ◽  
Vol 47 (10) ◽  
pp. 1396-1402 ◽  
Author(s):  
Carolina Gubert ◽  
Laura Stertz ◽  
Bianca Pfaffenseller ◽  
Bruna Schilling Panizzutti ◽  
Gislaine Tezza Rezin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Mitochondrial Activity ◽  
Oxidative Stress Markers ◽  
Peripheral Blood Mononuclear ◽  
Stress Markers ◽  
Blood Mononuclear Cells ◽  
And Oxidative Stress

scholarly journals Biochemical parameters and oxidative stress markers in Tunisian patients with periodontal disease

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Ahmed Gharbi ◽  
Ali Hamila ◽  
Adel Bouguezzi ◽  
Azza Dandana ◽  
Salima Ferchichi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Healthy Subjects ◽  
Biochemical Parameters ◽  
Periodontal Diseases ◽  
Enzymatic Antioxidants ◽  
Plasma Samples ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Abstract Background Oxidative stress is involved in many diseases including diabetes and cancer. Numbers of studies have suggested its involvement in the pathogenesis of periodontal diseases. The aim of this study was to evaluate the levels of biochemical parameters and oxidative stress markers in plasma of healthy and chronic periodontitis patients. Methods One hundred thirty subjects were divided into two groups; patients (mean age = 42 ± 13.6 y.o) and control (mean age = 44.8 ± 12.6 y.o). Patients and healthy subjects were free from any infection, coronary or heart disease, diabetes or liver failure. Total cholesterol, LDLc, HDLc, Triglycerides (TG), creatinine, uric acid (UA), glucose and urea levels as well as the activities of enzymatic antioxidants such as catalase, glutathione reductase (GR) and total antioxidant capacity (TAOC), were measured in plasma samples using colorimetric assays. Statistical differences between groups were determined by Student’s t-test and p ≤ 0.05 was considered as significant. Results Periodontitis patients exhibited significant decrease in the activities of catalase, TAOC, GR and TG, cholesterol, LDLc, glucose, HDLc, uric acid levels in plasma samples in comparison with healthy subjects. However, no statistically significant differences in the levels of creatinine and urea were observed between the two groups. Conclusion The reduction of plasma antioxidant activities (Catalase, TAOC, GR) may have a role in the pathogenesis of periodontal diseases. Our findings suggest a decrease in the host capacity to control the damage caused by oxidative stress. Therefore, therapeutic strategies, aiming at modulating the oxidative stress could be considered as potential tools for the prevention or treatment of periodontal diseases and their potential systemic effects on the general health.

Inflammatory and oxidative stress markers among children and adolescents with bipolar disorder

2017 ◽  
Vol 27 ◽  
pp. S21
Author(s):  
F. Hernandez Alvarez ◽  
G. Salazar de Pablo ◽  
R. Vicente Moreno ◽  
A. Del Rey Mejías ◽  
K. McDowell ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Children And Adolescents ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Iontophoresis with gold nanoparticles improves mitochondrial activity and oxidative stress markers of burn wounds

2014 ◽  
Vol 44 ◽  
pp. 380-385 ◽  
Author(s):  
Paulo C.L. Silveira ◽  
Mirelli Venâncio ◽  
Priscila S. Souza ◽  
Eduardo G. Victor ◽  
Frederico de Souza Notoya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Mitochondrial Activity ◽  
Oxidative Stress Markers ◽  
Burn Wounds ◽  
Stress Markers ◽  
And Oxidative Stress

Methamidophos induces cytotoxicity and oxidative stress in human peripheral blood mononuclear cells

2015 ◽  
Vol 32 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Marco Antonio Ramirez-Vargas ◽  
Gerardo Huerta-Beristain ◽  
Iris Paola Guzman-Guzman ◽  
Luz del Carmen Alarcon-Romero ◽  
Eugenia Flores-Alfaro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
And Oxidative Stress

scholarly journals Neurotrophins, cytokines, oxidative stress mediators and mood state in bipolar disorder: systematic review and meta-analyses

2018 ◽  
Vol 213 (3) ◽  
pp. 514-525 ◽  
Author(s):  
Tobias Rowland ◽  
Benjamin I. Perry ◽  
Rachel Upthegrove ◽  
Nicholas Barnes ◽  
Jayanta Chatterjee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Bipolar Disorder ◽  
Inflammatory Markers ◽  
Healthy Controls ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Tnf Α ◽  
Meta Analyses ◽  
And Oxidative Stress

BackgroundA reliable biomarker signature for bipolar disorder sensitive to illness phase would be of considerable clinical benefit. Among circulating blood-derived markers there has been a significant amount of research into inflammatory markers, neurotrophins and oxidative stress markers.AimsTo synthesise and interpret existing evidence of inflammatory markers, neurotrophins and oxidative stress markers in bipolar disorder focusing on the mood phase of illness.MethodFollowing PRISMA (Preferred Reporting Items for Systematic reviews and Meta-analyses) guidelines, a systematic review was conducted for studies investigating peripheral biomarkers in bipolar disorder compared with healthy controls. We searched Medline, Embase, PsycINFO, SciELO and Web of Science, and separated studies by bipolar mood phase (mania, depression and euthymia). Extracted data on each biomarker in separate mood phases were synthesised using random-effects model meta-analyses.ResultsIn total, 53 studies were included, comprising 2467 cases and 2360 controls. Fourteen biomarkers were identified from meta-analyses of three or more studies. No biomarker differentiated mood phase in bipolar disorder individually. Biomarker meta-analyses suggest a combination of high-sensitivity C-reactive protein/interleukin-6, brain derived neurotrophic factor/tumour necrosis factor (TNF)-α and soluble TNF-α receptor 1 can differentiate specific mood phase in bipolar disorder. Several other biomarkers of interest were identified.ConclusionsCombining biomarker results could differentiate individuals with bipolar disorder from healthy controls and indicate a specific mood-phase signature. Future research should seek to test these combinations of biomarkers in longitudinal studies.Declaration of interestNone.

scholarly journals Elevated extracellular CO2 level affects the adaptive transcriptional response and survival of human peripheral blood mononuclear cells toward hypoxia and oxidative stress

2021 ◽  
Vol 30 (1) ◽  
pp. 5-12
Author(s):  
Septelia Inawati Wanandi ◽  
Sekar Arumsari ◽  
Edwin Afitriansyah ◽  
Resda Akhra Syahrani ◽  
Idham Rafly Dewantara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Transcriptional Response ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Co2 Level ◽  
And Oxidative Stress

BACKGROUND High carbon dioxide (CO2) level from indoor environments, such as classrooms and offices, might cause sick building syndrome. Excessive indoor CO2 level increases CO2 level in the blood, and over-accumulation of CO2 induces an adaptive response that requires modulation of gene expression. This study aimed to investigate the adaptive transcriptional response toward hypoxia and oxidative stress in human peripheral blood mononuclear cells (PBMCs) exposed to elevated CO2 level in vitro and its association with cell viability. METHODS PBMCs were treated in 5% CO2 and 15% CO2, representatives a high CO₂ level condition for 24 and 48 hours. Extracellular pH (pHe) was measured with a pH meter. The levels of reactive oxygen species were determined by measuring superoxide and hydrogen peroxide with dihydroethidium and dichlorofluorescin-diacetate assay. The mRNA expression levels of hypoxia-inducible factor (HIF)-1α, HIF-2α, nuclear factor (NF)-κB, and manganese superoxide dismutase (MnSOD) were analyzed using a real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell survival was determined by measuring cell viability. RESULTS pHe increased in 24 hours after 15% CO₂ treatment, and then decreased in 48 hours. Superoxide and hydrogen peroxide levels increased after the 24- and 48-hour of high CO₂ level condition. The expression levels of NF-κB, MnSOD, HIF-1α, and HIF-2α decreased in 24 hours and increased in 48 hours. The increased antioxidant mRNA expression in 48 hours showed that the PBMCs were responsive under high CO2 conditions. Elevated CO2 suppressed cell viability significantly in 48 hours. CONCLUSIONS After 48 hours of high CO₂ level condition, PBMCs showed an upregulation in genes related to hypoxia and oxidative stress to overcome the effects of CO2 elevation.

scholarly journals High BDNF serum levels are associated to good cognitive functioning in bipolar disorder

2019 ◽  
Vol 60 ◽  
pp. 97-107 ◽  
Author(s):  
Ester Mora ◽  
Maria J. Portella ◽  
Gerard Piñol-Ripoll ◽  
Ricard López ◽  
Daniel Cuadras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Cognitive Functioning ◽  
Verbal Memory ◽  
Cognitive Outcome ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Bipolar Patients ◽  
And Oxidative Stress

AbstractBackground:Neurotrophins such as brain-derived neurotrophic factor (BDNF), inflammation and oxidative damage may contribute to the pathophysiology of bipolar disorder (BD) in terms of illness activity. To date, there is a lack of studies linking the cognitive impairment observed in BD with these neurobiological mechanisms. This study aimed to investigate the role of these neurobiological factors in clinical and cognitive outcomes in a sample of bipolar individuals.Methods:We measured serum BDNF, cytokines and oxidative stress markers in a sample of 133 individuals: 52 euthymic bipolar patients, 32 manic patients and 49 healthy controls. They were all assessed with a comprehensive cognitive battery. Sociodemographic and clinical data were collected. Multiple linear regression models were built to study associations of neurotrophins and inflammatory and oxidative measures with cognitive functioning.Results:BDNF levels were decreased in euthymic (p = 0.039) and manic (p < 0.001) individuals. Conversely, inflammatory (interleukin 6 (IL-6)) (p = 0.019) and oxidative stress (p = 0.003) measures were increased in bipolar individuals compared to controls. BDNF levels were associated with executive functioning (β = 0.01, p = 0.02) and verbal memory (β = 0.013, p = 0.005), together with other demographic variables. In particular, verbal memory was also associated with obesity (β=-0.04, p = 0.005). Neither inflammatory markers, oxidative stress markers nor other relevant clinical variables showed any association with cognitive outcome.Conclusions:Of all the peripheral neurobiological factors analysed, BDNF was the only one significantly associated with cognitive dysfunction in bipolar disorder individuals. This study emphasizes the role of BDNF not only across mood phases but also in cognitive functioning.

Oxidative DNA damage in equine peripheral blood mononuclear cells (PBMC) induced by acute natural allergen challenge

2004 ◽  
Vol 32 ◽  
pp. 189-190
Author(s):  
D A Kingston ◽  
D J Marlin ◽  
N C Smith ◽  
C M Deaton ◽  
K Saunders ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Oxidative Dna Damage ◽  
Mononuclear Cells ◽  
Allergen Challenge ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
And Oxidative Stress

Oxidative stress occurs when antioxidant defence mechanisms are overwhelmed by free radicals and may lead to damage to DNA, which has been implicated in processes such as ageing and cancer. The Comet assay allows detection of oxidative DNA damage in individual cells. As horses with recurrent airway obstruction (RAO) have been shown to demonstrate low antioxidant status and oxidative stress, we hypothesised that peripheral blood mononuclear cells (PBMC) of horses with RAO would demonstrate increases in DNA damage following natural allergen challenge.Six horses (mean age 15 years, range 8-23 years) diagnosed with RAO (in remission) and 6 healthy breed matched controls (mean age 9 years, range 5-15 years) were studied. Blood samples were collected 7 days prior to challenge and immediately and 3 days after stabling on mouldy hay and straw for 24h. All animals were kept at grass prior to and after the challenge period. Bronchoalveolar lavage (BAL) was performed and neutrophil counts determined.

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