In-vitro bioactivity evaluation and non-targeted metabolomic analysis of green tea processed from different tea shoot maturity

Temulawak (Curcuma xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol

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N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

Current Biochemistry ◽

10.29244/cb.6.2.3 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

Lisni Noraida Waruwu ◽

Maria Bintang ◽

Bambang Pontjo Priosoeryanto

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Green Tea ◽

Cancer Cell ◽

Breast Cancer Cells ◽

Green Tea Extract ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro

Antiviral Research ◽

10.1016/j.antiviral.2007.11.011 ◽

2008 ◽

Vol 78 (3) ◽

pp. 242-249 ◽

Cited By ~ 54

Author(s):

Jun Xu ◽

Jue Wang ◽

Fei Deng ◽

Zhihong Hu ◽

Hualin Wang

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Green Tea Extract ◽

B Virus ◽

Tea Extract

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Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water

Waste and Biomass Valorization ◽

10.1007/s12649-021-01477-z ◽

2021 ◽

Author(s):

Jaroslava Švarc-Gajić ◽

Víctor Cerdà ◽

Cristina Delerue-Matos ◽

Pavle Mašković ◽

Sabrina Clavijo ◽

...

Keyword(s):

Subcritical Water ◽

Chemical Characterization ◽

In Vitro Bioactivity

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Phytochemical investigations and in vitro bioactivity screening on Melia azedarach L. leaves extract from Nepal

Chemistry & Biodiversity ◽

10.1002/cbdv.202001070 ◽

2021 ◽

Author(s):

Stefano Dall'Acqua ◽

Shyam Sharan Shrestha ◽

Irene Ferrarese ◽

Stefania Sut ◽

Gokhan Zengin ◽

...

Keyword(s):

Melia Azedarach ◽

In Vitro Bioactivity

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Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles

Gels ◽

10.3390/gels7020034 ◽

2021 ◽

Vol 7 (2) ◽

pp. 34

Author(s):

Shaher Bano ◽

Memoona Akhtar ◽

Muhammad Yasir ◽

Muhammad Salman Maqbool ◽

Akbar Niaz ◽

...

Keyword(s):

Bioactive Glass ◽

Composite Coatings ◽

Antibacterial Properties ◽

Bone Diseases ◽

Osteogenic Potential ◽

Diffusion Method ◽

Charge Composition ◽

In Vitro Bioactivity ◽

Mesoporous Bioactive Glass

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

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Mesoporous Properties of Bioactive Glass Synthesized by Spray Pyrolysis with Various Polyethylene Glycol and Acid Additions

Polymers ◽

10.3390/polym13040618 ◽

2021 ◽

Vol 13 (4) ◽

pp. 618

Author(s):

Tzu-Yu Peng ◽

Pei-Yun Tsai ◽

May-Show Chen ◽

Yuichi Mine ◽

Shan-Hua Wu ◽

...

Keyword(s):

Molecular Weight ◽

Polyethylene Glycol ◽

Bioactive Glass ◽

Spray Pyrolysis ◽

Mesoporous Structure ◽

High Specific Surface Area ◽

Process Time ◽

In Vitro Bioactivity ◽

One Pot

Mesoporous bioactive glass (MBG) has a high specific surface area, promoting the reaction area, thereby improving the bioactivity; thus, MBG is currently gaining popularity in the biomaterial field. Spray pyrolysis (SP) is a one-pot process that has the advantages of shorter process time and better particle bioactivity, therefore, MBG was prepared by SP process with various polyethylene glycol (PEG, molecular weight ranged from 2000–12,000) and acid (HCl and CH3COOH) additions. In vitro bioactivity and mesoporous properties of the so-obtained MBG were investigated. The experimental results showed that all the MBG exhibited amorphous and mesoporous structure. Increasing the molecular weight of PEG can improve the mesoporous structure and bioactivity of MBG. Whereas optimized MBG was prepared with suitable concentration of PEG and CH3COOH. In the present work, MBG synthesized via spray pyrolysis by adding 5 g of PEG with a molecular weight of 12,000 and 50 mL of CH3COOH exhibited the best in vitro bioactivity and mesoporous structure.

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