Identification of differentially expressed genes in the hydrothermal vent shrimp Rimicaris exoculata exposed to heat stress

2010 ◽  
Vol 3 (2) ◽  
pp. 71-78 ◽  
Author(s):  
Delphine Cottin ◽  
Bruce Shillito ◽  
Thomas Chertemps ◽  
Arnaud Tanguy ◽  
Nelly Léger ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Hydrothermal Vent ◽  
Differentially Expressed ◽  
Rimicaris Exoculata

scholarly journals The Algal Symbiont Modifies the Transcriptome of the Scleractinian Coral Euphyllia paradivisa During Heat Stress

2019 ◽  
Vol 7 (8) ◽  
pp. 256 ◽  
Author(s):  
Dalit ◽  
Keren ◽  
Eviatar ◽  
Hiba ◽  
Gal ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Elevated Temperatures ◽  
Negative Impact ◽  
Global Climate ◽  
Differentially Expressed ◽  
Heat Stress Response ◽  
Stress Genes ◽  
Mutualistic Symbiosis ◽  
Algal Symbiont

The profound mutualistic symbiosis between corals and their endosymbiotic counterparts, Symbiodiniaceae algae, has been threatened by the increase in seawater temperatures, leading to breakdown of the symbiotic relationship—coral bleaching. To characterize the heat-stress response of the holobiont, we generated vital apo-symbiotic Euphyllia paradivisa corals that lacked the endosymbiotic algae. Using RNA sequencing, we analyzed the gene expression of these apo-symbionts vs. symbiotic ones, to test the effect of the algal presence on the tolerance of the coral. We utilized literature-derived lists of “symbiosis differentially expressed genes” and “coral heat-stress genes” in order to compare between the treatments. The symbiotic and apo-symbiotic samples were segregated into two separate groups with several different enriched gene ontologies. Our findings suggest that the presence of endosymbionts has a greater negative impact on the host than the environmental temperature conditions experienced by the holobiont. The peak of the stress reaction was identified as 28 °C, with the highest number of differentially expressed genes. We suggest that the algal symbionts increase coral holobiont susceptibility to elevated temperatures. Currently, we can only speculate whether coral species, such as E. paradivisa, with the plasticity to also flourish as apo-symbionts, may have a greater chance to withstand the upcoming global climate change challenge.

scholarly journals Expression Profiling of Heat Shock Protein Genes as Putative Early Heat-Responsive Members in Lettuce

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 312
Author(s):  
Yeeun Kang ◽  
Suk-Woo Jang ◽  
Hee Ju Lee ◽  
Derek W. Barchenger ◽  
Seonghoe Jang
Keyword(s):  
Heat Treatment ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Differentially Expressed Genes ◽  
Expression Patterns ◽  
Differentially Expressed ◽  
Cool Season ◽  
Potential Link ◽  
Hsp Genes

High temperatures due to global warming can cause harmful effects on the productivity of lettuce, a cool-season crop. To identify lettuce heat shock protein (HSP) genes that could be involved in early responses to heat stress in plants, we compared RNA transcriptomes between lettuce plants with and without heat treatment of 37 °C for 1 h. Using transcriptome sequencing analyses, a total of 7986 differentially expressed genes (DEGs) were identified including the top five, LsHSP70A, LsHSP70B, LsHSP17.3A, LsHSP17.9A and LsHSP17.9B, which were the most highly differentially expressed genes. In order to investigate the temporal expression patterns of 24 lettuce HSP genes with a fold-change greater than 100 under heat stress, the expression levels of the genes were measured by qRT-PCR at 0, 1, 4, 8, 14, and 24 h time points after heat treatment. The 24 LsHSP genes were classified into three groups based on the phylogenetic analysis and/or major domains available in each protein, and we provided a potential link between the phylogenetic relationships and expression patterns of the LsHSP genes. Our results showed putative early heat-responsive lettuce HSP genes that could be possible candidates as breeding guides for the development of heat-tolerant lettuce cultivars.

scholarly journals Exploring differentially expressed genes of microspore embryogenesis under heat stress in sweet pepper

2020 ◽  
Vol 19 (9) ◽  
pp. 661-674
Author(s):  
Cheng Yan ◽  
Jiao Yansheng ◽  
Miao Ruyi ◽  
Tian Ruxia ◽  
Liang Yanping ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Microspore Embryogenesis ◽  
Sweet Pepper ◽  
Differentially Expressed

scholarly journals Transcriptomic Analysis Provides Novel Insights into Heat Stress Responses in Sheep

Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 387 ◽  
Author(s):  
Zengkui Lu ◽  
Mingxing Chu ◽  
Qing Li ◽  
Meilin Jin ◽  
Xiaojuan Fei ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Stress Responses ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Transcriptomic Analysis ◽  
Differentially Expressed ◽  
P Value ◽  
Comprehensive Overview

With the intensified and large-scale development of sheep husbandry and global warming, sheep heat stress has become an increasingly important issue. However, little is known about the molecular mechanisms related to sheep responses to heat stress. In this study, transcriptomic analysis of liver tissues of sheep in the presence and absence of heat stress was conducted, with the goal of identifying genes and pathways related to regulation when under such stress. After a comparison with the sheep reference genome, 440,226,436 clean reads were obtained from eight libraries. A p-value ≤ 0.05 and fold change ≥ 2 were taken as thresholds for categorizing differentially expressed genes, of which 1137 were identified. The accuracy and reliability of the RNA-Seq results were confirmed by qRT-PCR. The identified differentially expressed genes were significantly associated with 419 GO terms and 51 KEGG pathways, which suggested their participation in biological processes such as response to stress, immunoreaction, and fat metabolism. This study’s results provide a comprehensive overview of sheep heat stress-induced transcriptional expression patterns, laying a foundation for further analysis of the molecular mechanisms of sheep heat stress.

Identification of differentially expressed genes in Chrysanthemum nankingense (Asteraceae) under heat stress by RNA Seq

Gene ◽  
2014 ◽  
Vol 552 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Jing Sun ◽  
Liping Ren ◽  
Yue Cheng ◽  
Jiaojiao Gao ◽  
Bin Dong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Rna Seq

scholarly journals Microarray Analysis of Gene Expression Profiles of Rat Small Intestine in Response to Heat Stress

2011 ◽  
Vol 16 (6) ◽  
pp. 655-667 ◽  
Author(s):  
An Lu ◽  
Huichuan Wang ◽  
Xiaolin Hou ◽  
Huanrong Li ◽  
Guilin Cheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Treatment ◽  
Small Intestine ◽  
Heat Stress ◽  
Differentially Expressed Genes ◽  
Microarray Analysis ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Epithelial Tissue ◽  
Rat Small Intestine

Ambient temperature is a critical factor that affects biological organisms in many ways. In this study, the authors investigated gene expression changes in rat small intestine in response to heat stress. Male Sprague-Dawley rats were randomly divided into control and heat-stressed groups. Both groups were housed at 25 °C, although the heat-stressed group was also subjected to 40 °C for 2 h each day for 10 successive days. Rats were sacrificed 1, 3, 6, and 10 days after heat treatment, and sections of their small intestine epithelial tissue were excised for morphological examination and microarray analyses. The rat rectal and body surface temperatures and serum cortisol levels were all significantly increased after heat treatment (p < 0.05). The jejuna were significantly damaged by 3 days after heat treatment began. Microarray analysis showed that 422 genes were differentially expressed, of which 290 genes were significantly upregulated and 132 genes were significantly downregulated. Subsequent bioinformatics analyses revealed that the differentially expressed genes were mainly related to stress, immune regulation, and metabolism processes. The bioinformatics analysis of the differentially expressed genes should be beneficial to further investigations on the underlying mechanisms involved in heat stress–induced damage in the small intestine.

scholarly journals Comparative Transcriptome Analysis of the Phototrophic Dinoflagellate Biecheleriopsis adriatica Grown Under Optimal Temperature and Cold and Heat Stress

2021 ◽  
Vol 8 ◽  
Author(s):  
Hee Chang Kang ◽  
Hae Jin Jeong ◽  
Sang Ah Park ◽  
Jin Hee Ok ◽  
Ji Hyun You ◽  
...  
Keyword(s):  
Heat Stress ◽  
Cold Stress ◽  
Water Temperature ◽  
Differentially Expressed Genes ◽  
Temperature Fluctuations ◽  
Hot Water ◽  
Differentially Expressed ◽  
Optimal Temperature ◽  
Number Of Genes ◽  
Upregulated Genes

Dinoflagellates are a major component of marine ecosystems, and very cold and hot water may affect their survival. Global warming has amplified the magnitude of water temperature fluctuations. To investigate the molecular responses of dinoflagellates to very cold and hot water, we compared the differentially expressed genes of the phototrophic dinoflagellate Biecheleriopsis adriatica grown under optimal temperature and cold and heat stress. The number of genes upregulated or downregulated between optimal temperature and cold stress was twice than that between optimal temperature and heat stress. Moreover, the number of upregulated genes was greater than that of the downregulated genes under cold stress, whereas the number of upregulated genes was less than that of the downregulated genes under heat stress. Furthermore, among the differentially expressed genes, the number of genes upregulated under cold stress and with unchanged expression under heat stress was the highest, while the number of the genes downregulated under cold stress, but not under heat stress, was the second-highest. Facilitated trehalose transporter Tret1 and DnaJ-like subfamily B member 6-A were upregulated and downregulated, respectively, under cold stress; however, their expression remained unchanged under heat stress. In contrast, Apolipoprotein d lipocalin and Troponin C in skeletal muscle were upregulated and downregulated, respectively, under both cold and heat stress. This study provides insight into the genetic responses of dinoflagellates to climate change-driven large water temperature fluctuations.

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