The cell structure damage and embden-meyerhof-parnas pathway inhibition of Listeria monocytogenes induced by glycinin basic peptide

2020 ◽  
pp. 104635
Author(s):  
Houqi Ning ◽  
Shuangtong Wang ◽  
Yingqiu Li ◽  
Guijin Sun ◽  
Jinxing He
LWT ◽  
2019 ◽  
Vol 99 ◽  
pp. 62-68 ◽  
Author(s):  
Hou-Qi Ning ◽  
Ying-Qiu Li ◽  
Qi-Wen Tian ◽  
Zhao-Sheng Wang ◽  
Hai-Zhen Mo

2017 ◽  
Vol 65 (25) ◽  
pp. 5173-5180 ◽  
Author(s):  
Guo-Ping Zhao ◽  
Ying-Qiu Li ◽  
Gui-Jin Sun ◽  
Hai-Zhen Mo

LWT ◽  
2021 ◽  
pp. 111977
Author(s):  
Shuang-Tong Wang ◽  
Hou-Qi Ning ◽  
Lin-Hui Feng ◽  
Ying-Ying Wang ◽  
Ying-Qiu Li ◽  
...  

2018 ◽  
Vol 14 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Linhui Feng ◽  
Yingqiu Li ◽  
Zhaosheng Wang ◽  
Lianqing Qi ◽  
Haizhen Mo

Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.


Author(s):  
D. Caillard ◽  
J.L. Martin

The behaviour of the dislocation substructure during the steady stage regime of creep, as well as its contribution to the creep rate, are poorly known. In particular, the stability of the subboundaries has been questioned recently, on the basis of experimental observations |1||2| and theoretical estimates |1||3|. In situ deformation experiments in the high voltage electron microscope are well adapted to the direct observation of this behaviour. We report here recent results on dislocation and subboundary properties during stationary creep of an aluminium polycristal at 200°C.During a macroscopic creep test at 200°C, a cell substructure is developed with an average cell size of a few microns. Microsamples are cut out of these specimens |4| with the same tensile axis, and then further deformed in the microscope at the same temperature and stain rate. At 1 MeV, one or a few cells can be observed in the foil thickness |5|. Low electron fluxes and an image intensifier were used to reduce radiation damage effects.


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