The Synergistic Antibacterial Properties of Glycinin Basic Peptide against Bacteria via Membrane Damage and Inactivation of Enzymes

ABSTRACT Totarol is a natural antimicrobial compound extracted from the heartwood of Podocarpus totara, a conifer native to New Zealand. The effects of whey protein–totarol nanoparticles treated with ultrasound on the physiochemical properties and the growth of Staphylococcus aureus were investigated. The particle size of whey protein–totarol nanoparticles was reduced by ultrasound treatment from 31.24 ± 5.31 to 24.20 ± 4.02 nm, and the size distribution was also narrowed by the treatment. Viscosity and modulus data indicated that the flow behaviors of whey protein–totarol nanoparticles seemed to be Newtonian and exerted a typical viscoelastic fluid at protein content of 15% (w/v). Rheological properties were more insensitive to ultrasonic time. Time-killing assays, agar diffusion tests, the cell membrane damage analysis, and microstructure were exploited to study the antibacterial properties of whey protein–totarol nanoparticles. The MIC of whey protein–totarol nanoparticles after ultrasound treatment decreased from 4 to 2 μg/mL compared with that without ultrasound treatment. Whey protein–totarol nanoparticles treated with ultrasound resulted in a significant (P < 0.05) decrease in time killing after 24 h. The agar diffusion results showed that the inhibition zones of whey protein–totarol nanoparticles were 12 and 36 mm for untreated and treated with ultrasound, respectively. The cell membrane damages and the microstructure changes also proved that whey protein–totarol nanoparticles treated with ultrasound had strong antibacterial activities against S. aureus and that the antibacterial effectiveness enhanced with the increasing of ultrasonic time. These findings suggested that whey protein–totarol nanoparticles treated with ultrasound were more effective against S. aureus than untreated nanoparticles.

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A Novel Fluorescence-Labeled Curcumin Conjugate: Synthesis, Evaluation and Imaging on Human Cell Lines

Current Pharmaceutical Design ◽

10.2174/1381612824666180406103317 ◽

2018 ◽

Vol 24 (16) ◽

pp. 1821-1826 ◽

Cited By ~ 2

Author(s):

Sumbla Sheikh ◽

Alexander Sturzu ◽

Hubert Kalbacher ◽

Thomas Nagele ◽

Christopher Weidenmaier ◽

...

Keyword(s):

Cell Growth ◽

Cell Lines ◽

Human Cell ◽

Cellular Localization ◽

Membrane Damage ◽

Antibacterial Properties ◽

Water Soluble ◽

Osteosarcoma Cell ◽

Human Cell Lines

Curcumin, as the main ingredient of the curcuma spice, has increasingly become the target of scientific research. The turmeric root where the spice is obtained from has been widely used in the traditional medicine. Moreover, scientific studies have found that curcumin has anti-inflammatory, anti-cancer, anti-angiogenic effects as well as antibacterial properties. Recently, curcumin has gathered interest as a potential therapeutic agent in the research on Alzheimer’s disease. A consistent problem in the investigative and therapeutic applications of curcumin is its poor solubility in aqueous solutions. In the present study, we synthesized a conjugate of curcumin, the amino acid lysine and the fluorescent dye fluorescein. This conjugate was soluble in cell culture medium and facilitated the examination of curcumin with fluorescence imaging methods. We studied the cell growth impact of unmodified curcumin on seven different human cell lines and then analyzed the uptake and cellular localization of our curcumin conjugate with confocal laser scanning imaging and flow cytometry on the seven cell lines. We found that unbound curcumin inhibited cell growth in vitro and was not taken up into the cells. The curcumin conjugate was internalized into the cell cytoplasm in a dot-like pattern and cellular uptake correlated with the cell membrane damage which was measured using propidium iodide. The CAL-72 osteosarcoma cell exhibited 3-4fold increased conjugate uptake and a strong uniform fluorescein staining in addition to the dot-like pattern observed in all cell lines. In conclusion, we successfully synthesized a novel water-soluble fluorescent curcumin conjugate which showed a strong preference for CAL-72 osteosarcoma cells in vitro.

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The apoptosis of Staphylococcus aureus induced by glycinin basic peptide through ROS oxidative stress response

LWT ◽

10.1016/j.lwt.2018.09.028 ◽

2019 ◽

Vol 99 ◽

pp. 62-68 ◽

Cited By ~ 3

Author(s):

Hou-Qi Ning ◽

Ying-Qiu Li ◽

Qi-Wen Tian ◽

Zhao-Sheng Wang ◽

Hai-Zhen Mo

Keyword(s):

Oxidative Stress ◽

Staphylococcus Aureus ◽

Stress Response ◽

Oxidative Stress Response ◽

Basic Peptide ◽

Glycinin Basic Peptide

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Antibacterial Actions of Glycinin Basic Peptide against Escherichia coli

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.7b02295 ◽

2017 ◽

Vol 65 (25) ◽

pp. 5173-5180 ◽

Cited By ~ 13

Author(s):

Guo-Ping Zhao ◽

Ying-Qiu Li ◽

Gui-Jin Sun ◽

Hai-Zhen Mo

Keyword(s):

Escherichia Coli ◽

Basic Peptide ◽

Glycinin Basic Peptide

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Oxidative phosphorylation system as the target of glycinin basic peptide against Aspergillus niger

LWT ◽

10.1016/j.lwt.2021.111977 ◽

2021 ◽

pp. 111977

Author(s):

Shuang-Tong Wang ◽

Hou-Qi Ning ◽

Lin-Hui Feng ◽

Ying-Ying Wang ◽

Ying-Qiu Li ◽

...

Keyword(s):

Aspergillus Niger ◽

Oxidative Phosphorylation ◽

Basic Peptide ◽

Oxidative Phosphorylation System ◽

Glycinin Basic Peptide

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Antifungal Actions of Glycinin Basic Peptide against Aspergillus niger through the Collaborative Damage to Cell Membrane and Mitochondria

Food Biophysics ◽

10.1007/s11483-018-9561-4 ◽

2018 ◽

Vol 14 (1) ◽

pp. 97-107 ◽

Cited By ~ 2

Author(s):

Linhui Feng ◽

Yingqiu Li ◽

Zhaosheng Wang ◽

Lianqing Qi ◽

Haizhen Mo

Keyword(s):

Aspergillus Niger ◽

Cell Membrane ◽

Basic Peptide ◽

Glycinin Basic Peptide

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Mechanisms Mediating Bactericidal Properties and Conditions That Enhance the Potency of a Broad-Spectrum Oligo-Acyl-Lysyl

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00666-10 ◽

2010 ◽

Vol 55 (2) ◽

pp. 688-695 ◽

Cited By ~ 15

Author(s):

Hadar Sarig ◽

Yair Goldfeder ◽

Shahar Rotem ◽

Amram Mor

Keyword(s):

Membrane Damage ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Bactericidal Effect ◽

Cell Membrane Permeability ◽

Permeability Barrier ◽

Host Defense Peptides ◽

Bactericidal Properties ◽

Time Kill

ABSTRACTPrevious studies have established the potential of the oligo-acyl-lysyl (OAK) concept in generating simple chemical mimics of host defense peptides (HDPs) with improved antimicrobial properties. We investigated the antibacterial properties of such an OAK, C16(ω7)-KK-C12-Kamide, to obtain a better understanding of the complex mode(s) of action of cationic antibacterial peptides. The average MIC, determined against a multispecies panel of 50 strains, was 6 ± 5 μg/ml. However, although the OAK exerted an essentially dose-dependent bactericidal effect (time-kill curves typically exhibited 99% death within 2 h), marked differences in the killing rates occurred among inter- and intraspecies strains. Mechanistic comparison between equally sensitive and related strains revealed death of one strain to stem from the OAK's capacity to breach the cell membrane permeability barrier, whereas the death of the related strain resulted from the OAK's direct interference with DNA functionsin vivo, without detectable membrane damage. These findings therefore support the notion that the antibacterial mechanism of action of a single HDP can vary among inter- and intraspecies strains. In addition, we present data illustrating the differential effects of environmental conditions (pH, ionic strength and temperature), on the OAK's antibacterial properties, and ultimately demonstrate potency enhancement (by orders of magnitude) through selection of optimal incubation conditions. Such attributes might be useful in a variety of antibacterial applications.

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Antibacterial Potential of a Novel Peptide from the Consensus sequence of Dermaseptin Related Peptides Secreted by Agalychnis annae

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666201020161428 ◽

2020 ◽

Vol 21 ◽

Author(s):

Ya’u Sabo Ajingi ◽

Auwal Muhammad ◽

Pongsak Khunrae ◽

Triwit Rattanarojpong ◽

Kovit Pattanapanyasate ◽

...

Keyword(s):

Bioactive Compounds ◽

Bacterial Resistance ◽

Consensus Sequence ◽

Membrane Damage ◽

Antibacterial Properties ◽

Peptide Sequence ◽

The Novel ◽

Bacterial Strains ◽

Skin Secretions ◽

Antibacterial Potential

Background: The consistently increasing reports of bacterial resistance and the reemergence of bacterial epidemics have inspired the health and scientific community to discover new molecules with antibacterial potential continuously. Frog-skin secretions constitute bioactive compounds essential for finding new biopharmaceuticals. The exact antibacterial characterization of dermaseptin related peptides derived from Agalychnis annae is limited. The resemblance in their conserved and functionally linked genomes indicates an unprecedented opportunity to obtain novel bioactive compounds. Objective: In this study, we derived a novel peptide sequence and determined its antibacterial potentials. Method: Consensus sequence strategy was used to design the novel and active antibacterial peptide named 'AGAAN' from skin secretions of Agalychnis annae. The In-vitro activities of the novel peptide against some bacterial strains were investigated. Time kill studies, DNA retardation, cytotoxicity, beta-galactosidase, and molecular computational studies were conducted. Results: AGAAN inhibited P. aeruginosa, E. faecalis, and S. typhimurium at 20 µM concentration. E. coli and S. aureus were inhibited at 25 µM, and lastly, B. subtilis at 50 µM. Kinetics of inactivation against exponential and stationary growing bacteria was found to be rapid within 1-5 hours of peptide exposure, depending on time and concentration. The peptide displayed weak hemolytic activity between 0.01%–7.31% at the antibacterial concentrations. AGAAN efficiently induced bacterial membrane damage with subsequent cell lysis. The peptide's DNA binding shows that it also targets intracellular DNA by retarding its movement. Our in-silico molecular docking analysis displayed a strong affinity to the bacterial cytoplasmic membrane. Conclusion: AGAAN exhibits potential antibacterial properties that could be used to combat bacterial resistance.

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