A novel direct contact method for the assessment of the antimicrobial activity of dental cements

2013 ◽  
Vol 93 (3) ◽  
pp. 168-172 ◽  
Author(s):  
E.M. Costa ◽  
S. Silva ◽  
A.R. Madureira ◽  
A. Cardelle-Cobas ◽  
F.K. Tavaria ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Contact Method

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Duddi Narendra Nirupama ◽  
Mohan Thomas Nainan ◽  
Rajendran Ramaswamy ◽  
Sethumadhavan Muralidharan ◽  
Hulimangala Hosakote Lingareddy Usha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Microbial Growth ◽  
Antimicrobial Property ◽  
Recurrent Infection ◽  
Antimicrobial Effect ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

Synthesis of Bulk Metal Matrix Nanocomposites by Full Cavitation Solidification Method

2013 ◽  
Vol 545 ◽  
pp. 193-196 ◽  
Author(s):  
Payodhar Padhi ◽  
Biranchi Narayan Dash ◽  
Pragyan Mohanty ◽  
B.K. Satapathy ◽  
Sachikanta Kar
Keyword(s):  
Liquid Metal ◽  
Metal Matrix ◽  
Direct Contact ◽  
Spray Deposition ◽  
Nano Particles ◽  
Order Effects ◽  
Contact Method ◽  
Solidification Processing ◽  
Ultrasonic Probe ◽  
Metal Matrix Nanocomposites

There are several methods for the production of metal matrix nano composites including mechanical alloying, vertex process and spray deposition. However, the above processes are expensive. Solidification processing is a relatively cheaper route. However, during solidification processing nano particulates tend to agglomerate as a result of van der Waals forces and thus proper dispersion of the nano-particulate in metal matrix is a challenge. Many researchers dispersed nanoparticles in metal matrix by ultrasonic casting. However their technique has several drawbacks such as the oscillating probe, which is in direct contact with liquid metal, may dissolve in the liquid metal and contaminate it. Moreover, the extent of dispersion is not uniform. It is maximum near the probe and gradually decreases as one move away from the probe. Lastly in the method developed by them, the oscillating probe is removed from the liquid metal before cooling and solidification begin. It may lead to partial reagglomeration of nanoparticles. To overcome these difficulties a non-contact method, where the ultrasonic probe is not in direct contact with the liquid metal, was attempted to disperse Nano-sized Al2O3 particulates in aluminium matrix. In this method the mold was subjected to ultrasonic vibration. The crystallite size of Al2O3 was mostly below 10 nm. Nano composite having 1-1.5 wt. % of Al2O3 was cast. From HRTEM studies it is observed that the Al2O3 particles are distributed uniformly except the grain boundaries. In micro scale the hardness is uniform throughout the samples. This is due to cavitation process as well as pushing of the nano-particles during the growth of the grains. In the present study it accounts all first-order effects i.e., phase change, bubble dynamics, turbulent pressure fluctuations, and noncondensable gases for deaglomeration and distribution of particles throughout the domain to get uniform distributions.

scholarly journals Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juliana Leitzke Santos de Souza ◽  
Tomaz Alves ◽  
Laísa Camerini ◽  
Fernanda Nedel ◽  
Angela Diniz Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Direct Contact ◽  
Eucalyptus Grandis ◽  
Effective Control ◽  
Natural Polymers ◽  
Contact Test ◽  
Enamel Demineralization ◽  
Chitosan Films ◽  
Direct Contact Test

AbstractChitosan films containing distilled pyroligneous extracts of Eucalyptus grandis (DPEC), characterized and developed by Brazilian Agricultural Research Corporation—Embrapa Temperate Agriculture (EMBRAPA-CPACT), were evaluated for antimicrobial activity against Candida albicans, Streptococcus mutans, and Lactobacillus acidophilus by direct contact test. Further, their capacity for the prevention of teeth enamel demineralization and cytotoxicity in vitro were also determined. The natural polymers were tested at different concentrations (1500–7500 µg mL−1) and the formulation of an experimental fluoride varnish with antimicrobial activity was evaluated by direct contact test, whereas cytotoxicity was analyzed through the colorimetric MTT assay. Preliminary data showed no statistically significant differences in cytotoxicity to NIH/3T3 cell line when DPEC is compared to the control group. On the other hand, the antimicrobial capacity and demineralization effects were found between the test groups at the different concentrations tested. Chitosan films containing distilled pyroligneous extracts of E. grandis may be an effective control strategy to prevent biofilm formation related to dental caries when applied as a protective varnish. They may inhibit the colonization of oral microorganisms and possibly control dental caries through a decrease in pH and impairment of enamel demineralization.

scholarly journals Antibacterial Activity of Chlorhexidine Bigluconate in Relation to Biofilm Monocultures

2021 ◽  
Vol 6 (1) ◽  
pp. 184-193
Author(s):  
G. G. Chistyakova ◽  
◽  
G. A. Skorokhod ◽  
I. O. Pohodenko-Chudakova
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Statistical Processing ◽  
Single Species ◽  
Modern Medicine ◽  
Reduction Factor ◽  
The Body ◽  
Contact Method ◽  
E Coli ◽  
High Level

On the background of a high percentage of caries incidence, the prevalence of its complications is increasing – pulpitis, apical periodontitis. Teeth with complicated caries can cause odontogenic inflammatory processes in the maxillofacial region. Being foci of chronic infection and intoxication, they have an adverse effect on the body as a whole. In connection with the above, it is obvious that determining the optimal concentration of chlorhexidine bigluconate and exposure to the dentin of the crown part of the tooth in case of carious lesion of the latter is currently very important. The purpose of the study was to evaluate the antimicrobial activity of chlorhexidine (by suspension and contact method) in various concentrations in relation to monocultures of biofilm formed on tooth sections and in U-shaped 96-well plastic plates. Material and methods. In this work, one of the most relevant pathogens for modern medicine was Staphylococcus aureus, which was one of the most powerful pathogens that form biofilms, was used as a model. The antimicrobial activity of chlorhexidine bigluconate was evaluated on S. aureus and E. coli biofilm cultures formed on dental samples and in polystyrene plastic tablets. Antimicrobial activity was evaluated by the reduction factor was determined by the difference in the number of decimal logarithms of CFU/ml in the experiment compared to the control. The obtained data was subjected to statistical processing. Results and discussion. The study revealed that 2.0 % solution of chlorhexidine bigluconate had a high level of antibacterial activity against tablet biofilm cultures of S. aureus and E. coli. When it was exposed to S. aureus and E. coli tablet biofilm cultures with exposures of 30 and 60 seconds, statistically significant differences were found (p=0.02). Secondly, statistically significant differences in the effect of sensitivity of single-species biofilm cultures of dental samples to the effect of antiseptic at the specified concentration were determined (p=0.007). At the same time, the level of antibacterial activity of 2.0 % chlorhexidine bigluconate solution in respect of tablet biofilm cultures of S. aureus and E. coli was significantly higher than in biofilm cultures of S. aureus and E. coli of dental samples (p<0.05). Conclusion. The obtained results give grounds for using them in clinical practice in order to improve the effectiveness of caries treatment which will help reduce the number of complications and on the one hand, corresponds to the main direction of medicine ‒ prevention and on the other hand, increases the level of specialized medical care provided to the population as a whole

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