scholarly journals Antibacterial Activity of Chlorhexidine Bigluconate in Relation to Biofilm Monocultures

2021 ◽  
Vol 6 (1) ◽  
pp. 184-193
Author(s):  
G. G. Chistyakova ◽  
◽  
G. A. Skorokhod ◽  
I. O. Pohodenko-Chudakova
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Statistical Processing ◽  
Single Species ◽  
Modern Medicine ◽  
Reduction Factor ◽  
The Body ◽  
Contact Method ◽  
E Coli ◽  
High Level

On the background of a high percentage of caries incidence, the prevalence of its complications is increasing – pulpitis, apical periodontitis. Teeth with complicated caries can cause odontogenic inflammatory processes in the maxillofacial region. Being foci of chronic infection and intoxication, they have an adverse effect on the body as a whole. In connection with the above, it is obvious that determining the optimal concentration of chlorhexidine bigluconate and exposure to the dentin of the crown part of the tooth in case of carious lesion of the latter is currently very important. The purpose of the study was to evaluate the antimicrobial activity of chlorhexidine (by suspension and contact method) in various concentrations in relation to monocultures of biofilm formed on tooth sections and in U-shaped 96-well plastic plates. Material and methods. In this work, one of the most relevant pathogens for modern medicine was Staphylococcus aureus, which was one of the most powerful pathogens that form biofilms, was used as a model. The antimicrobial activity of chlorhexidine bigluconate was evaluated on S. aureus and E. coli biofilm cultures formed on dental samples and in polystyrene plastic tablets. Antimicrobial activity was evaluated by the reduction factor was determined by the difference in the number of decimal logarithms of CFU/ml in the experiment compared to the control. The obtained data was subjected to statistical processing. Results and discussion. The study revealed that 2.0 % solution of chlorhexidine bigluconate had a high level of antibacterial activity against tablet biofilm cultures of S. aureus and E. coli. When it was exposed to S. aureus and E. coli tablet biofilm cultures with exposures of 30 and 60 seconds, statistically significant differences were found (p=0.02). Secondly, statistically significant differences in the effect of sensitivity of single-species biofilm cultures of dental samples to the effect of antiseptic at the specified concentration were determined (p=0.007). At the same time, the level of antibacterial activity of 2.0 % chlorhexidine bigluconate solution in respect of tablet biofilm cultures of S. aureus and E. coli was significantly higher than in biofilm cultures of S. aureus and E. coli of dental samples (p<0.05). Conclusion. The obtained results give grounds for using them in clinical practice in order to improve the effectiveness of caries treatment which will help reduce the number of complications and on the one hand, corresponds to the main direction of medicine ‒ prevention and on the other hand, increases the level of specialized medical care provided to the population as a whole

Preparation of bioactive and antibacterial PMMA-based bone cement by modification with quaternary ammonium and alkoxysilane

2021 ◽  
pp. 088532822110044
Author(s):  
Haiyang Wang ◽  
Toshinari Maeda ◽  
Toshiki Miyazaki
Keyword(s):  
Antibacterial Activity ◽  
Methyl Methacrylate ◽  
Bone Cement ◽  
Setting Time ◽  
Antibacterial Properties ◽  
The Body ◽  
Apatite Formation ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria

Bone cement based on poly(methyl methacrylate) (PMMA) powder and methyl methacrylate (MMA) liquid is a very popular biomaterial used for the fixation of artificial joints. However, there is a risk of this cement loosening from bone because of a lack of bone-bonding bioactivity. Apatite formation in the body environment is a prerequisite for cement bioactivity. Additionally, suppression of infection during implantation is required for bone cements to be successfully introduced into the human body. In this study, we modified PMMA cement with γ-methacryloxypropyltrimetoxysilane and calcium acetate to introduce bioactive properties and 2-( tert-butylamino)ethyl methacrylate (TBAEMA) to provide antibacterial properties. The long-term antibacterial activity is attributed to the copolymerization of TBAEMA and MMA. As the TBAEMA content increased, the setting time increased and the compressive strength decreased. After soaking in simulated body fluid, an apatite layer was detected within 7 days, irrespective of the TBAEMA content. The cement showed better antibacterial activity against Gram-negative E. Coli than Gram-positive bacteria; however, of the Gram-positive bacteria investigated, B. subtilis was more susceptible than S. aureus.

scholarly journals Antibacterial activity of ginger extracts on bacteria isolated from digestive tract infection patients attended Muhoza Health Center

2020 ◽  
Vol 11 (2) ◽  
pp. 35-41
Author(s):  
Callixte Yadufashije ◽  
Adolyne Niyonkuru ◽  
Emanuel Munyeshyaka ◽  
Sibomana Madjidi ◽  
Joseph Mucumbitsi
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Tract Infection ◽  
Health Center ◽  
Digestive Tract ◽  
Inhibition Zone ◽  
Ginger Extract ◽  
E Coli ◽  
Shigella Spp ◽  
Stool Samples

Background: Ginger (Zingiber officinale) has been used for long time due to its potential antimicrobial activity against diversity of microbial pathogens. Aims and Objectives: The study was carried out to investigate the bacteria pathogens found in digestive tract infections and assess antimicrobial activities of ginger extract to identified bacteria. Materials and Methods: Bacteriological studies were carried out on stool samples from 30 patients attending Muhoza health center. Different types of bacteria were isolated from stool samples of digestive tract infection patients by using various methods such culture, biochemical test and antimicrobial activity of ginger extracts was analyzed at INES-Ruhengeri in clinical microbiology laboratory. Results: Study findings showed isolated bacteria and antibacterial activity of Ginger. Isolated bacteria and their percentages including Escherichia coli (46.6%) which is the predominant isolated bacteria, Salmonella species (33.33%), Enterobacter spp (10.0%), Shigella spp (6.6%) and Citrobacter (3.33%) which is the least isolated bacteria. Antibacterial activity of ginger was seen on isolated bacteria, as ethanol and methanol were used for ginger oil extraction, the antibacterial activity of ginger extracts using ethanol was seen on isolated bacteria such us Citrobacter spp with 14 mm of inhibition zone, Shigella spp with 12 mm, Salmonella with 11.1 mm, E. coli with 9.5 mm and Enterobacter spp which was seen to be resistant to ginger extract using ethanol with 0.66mm of inhibition zone. For methanol extracts antibacterial activity was seen as follows: Citrobacter spp at 12 mm, Shigella spp at 11 mm, E. coli at 8 mm, salmonella spp at 6.1 mm, and Enterobacter spp with 5 mm. Enterobacter spp was seen to be the most resistant bacteria in both extracts. Conclusion: Ginger has shown to have an antibacterial activity on bacteria isolated from digestive tract infected patients. It can be used as a medicine to treat these infections. Number of researches should be done to be sure on this reality of antibacterial activity of ginger.

scholarly journals Justification of sorption composite usage on the basis of nanodisperse silica for sepsis prevention in complex treatment of purulent-inflammatory processes

2019 ◽  
Vol 41 part 3 (2) ◽  
pp. 45-50
Author(s):  
O. A. Viltsaniuk ◽  
P. V. Belyaev ◽  
T. P. Osolodchenko ◽  
O. O. Viltsaniuk
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Statistical Processing ◽  
Toxic Substances ◽  
Adhesive Properties ◽  
Adhesion Properties ◽  
E Coli ◽  
Adhesion Ability ◽  
Treatment And Prevention ◽  
Inflammatory Processes

Introduction. The problem of purulent-inflammatory processes treatment and the prevention of sepsis remains one of the most pressing problems of surgery.Purpose of the study. Is to estimate the effectiveness of sorption composition with antimicrobial properties on the basis of nanodisperse silica on the adhesive properties of microorganisms and to substantiate the expediency of its use for the purulent and inflammatory processes treatment and prevention of sepsis.Materials and methods.To determine the antimicrobial activity and the influence of the antiseptics of decasan, myramistin, ethonium, chlorhexidine and the sorption on the microorganisms adhesion properties composition was used the method of diffusion in agar of the museum strains of microorganisms S. Aureus ATCC 25923, E. Coli ATCC 25922, R. Vulgaris 4636, R. Aureginosa ATCC 27853, S. AIbicans 855/653, SI. Perfringens 28, Peptostreptococcus anaerobicus 22, Bacteroides fragilis 13/83, and clinical strains of S. Aureus, R. Aeuroginosa, E. Coli. Adhesive properties of microorganisms were studied according to the Brillis method et al. (1986) with the subsequent statistical processing of the data.Results. Studies have shown that antiseptics have high antimicrobial activity and reduce the adhesion ability of microorganisms. But the use of only antiseptics or only antibiotics for the purification of purulent foci can lead to toxins absorption and tissue decay products from purulent foci. That processes could be the trigger mechanism for sepsis development. The sorption composition is able to reduce the adhesive activity of bacteria, allows to absorb toxic substances from pathological foci, what preventing their resorption and thereby prevent the development of sepsis.Conclusions. The composition made from sorbents and antiseptics is not inferior to antimicrobial activity to antiseptics, which can reduce the virulence of bacteria, by reducing their adhesive activity, and absorb toxins of microorganisms and tissue decomposition products on their surface, thus prevent their absorption, which will prevent the purulent-inflammatory process progression and sepsis development.Keywords: sorption composition, nanodisperse silica, antiseptics, adhesive properties of microorganisms, prevention of sepsis.

Girth body dimensions in men and women with seborrheic dermatitis of varying severity

2021 ◽  
Vol 27 (4) ◽  
pp. 22-27
Author(s):  
Ahmad Raed Khasawneh ◽  
I.V. Serheta ◽  
N.V. Belik ◽  
A.O. Dovhan ◽  
I.I. Zhuchenko
Keyword(s):  
Body Size ◽  
Seborrheic Dermatitis ◽  
Severe Disease ◽  
Statistical Processing ◽  
Modern Medicine ◽  
The Body ◽  
Control Group ◽  
Men And Women ◽  
Healthy Men ◽  
Body Sizes

Today in modern medicine the study of human health is reoriented to the individual principle, which is based on the identification and study of constitutionally determined patterns of manifestation of certain diseases. The purpose of the study is to establish and analyze the features of the girth body sizes in men and women with generalized fatty form of seborrheic dermatitis of varying severity. The comprehensive body size of 40 men and 40 young women (25-44 years) with generalized fatty seborrheic dermatitis (mild and severe) was determined. The control group consisted of the girth sizes of practically healthy men (n=82) and women (n=154) of the same age group, which were selected from the database of the research center National Pirogov Memorial Medical University. Statistical processing of body circumference was performed in the licensed package “Statistica 6.0” using non-parametric evaluation methods. As a result of studies in patients with seborrheic dermatitis of varying severity of men, compared with practically healthy men, found only greater values of the girth of the shoulder in a relaxed state and thighs, neck (only mild), shin in the upper part and waist (in both cases only with a severe degree), as well as smaller values of the girth of the shoulder in a tense state; and in patients of varying severity of women – greater values of the girth of the shoulder in a relaxed state, thighs, lower legs, neck, waist and all girths of the chest and both thighs (only severe), as well as smaller values of the girth of the hand (only with mild). In both men and women with seborrheic dermatitis, differences in girth body sizes are more pronounced in people with severe disease. Between men or women with seborrheic dermatitis of varying severity, there are no significant or trends in differences in girth body sizes. In the analysis of the manifestations of sexual dimorphism of the circumferential body size between men and women with seborrheic dermatitis found greater values in men with mild and severe disease of the upper extremities, hands, shin, feet and neck (in most cases more pronounced in representatives with mild severity), as well as only in men with mild severity – greater values of all chest girths. For a more correct understanding of changes in girth body sizes in Ukrainian men or women with seborrheic dermatitis of varying severity, it is necessary to analyze other constitutional parameters of the body.

scholarly journals 3-Amino-5-(indol-3-yl)methylene-4-oxo-2-thioxothiazolidine Derivatives as Antimicrobial Agents: Synthesis, Computational and Biological Evaluation

2020 ◽  
Vol 13 (9) ◽  
pp. 229
Author(s):  
Volodymyr Horishny ◽  
Victor Kartsev ◽  
Vasyl Matiychuk ◽  
Athina Geronikaki ◽  
Petrou Anthi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Minimal Bactericidal Concentration ◽  
Active Compounds ◽  
Hek 293 ◽  
E Coli ◽  
Thiazolyl Blue Tetrazolium Bromide

Herein we report the design, synthesis, computational, and experimental evaluation of the antimicrobial activity of fourteen new 3-amino-5-(indol-3-yl) methylene-4-oxo-2-thioxothiazolidine derivatives. The structures were designed, and their antimicrobial activity and toxicity were predicted in silico. All synthesized compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin and (for the majority of compounds) streptomycin. The most sensitive bacterium was S. aureus (American Type Culture Collection ATCC 6538), while L. monocytogenes (NCTC 7973) was the most resistant. The best antibacterial activity was observed for compound 5d (Z)-N-(5-((1H-indol-3-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)-4-hydroxybenzamide (Minimal inhibitory concentration, MIC at 37.9–113.8 μM, and Minimal bactericidal concentration MBC at 57.8–118.3 μM). Three most active compounds 5d, 5g, and 5k being evaluated against three resistant strains, Methicillin resistant Staphilococcus aureus (MRSA), P. aeruginosa, and E. coli, were more potent against MRSA than ampicillin (MIC at 248–372 μM, MBC at 372–1240 μM). At the same time, streptomycin (MIC at 43–172 μM, MBC at 86–344 μM) did not show bactericidal activity at all. The compound 5d was also more active than ampicillin towards resistant P. aeruginosa strain. Antifungal activity of all compounds exceeded those of the reference antifungal agents bifonazole (MIC at 480–640 μM, and MFC at 640–800 μM) and ketoconazole (MIC 285–475 μM and MFC 380–950 μM). The best activity was exhibited by compound 5g. The most sensitive fungal was T. viride (IAM 5061), while A. fumigatus (human isolate) was the most resistant. Low cytotoxicity against HEK-293 human embryonic kidney cell line and reasonable selectivity indices were shown for the most active compounds 5d, 5g, 5k, 7c using thiazolyl blue tetrazolium bromide MTT assay. The docking studies indicated a probable involvement of E. coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds.

scholarly journals Antibacterial Activity of Soap Formulated from Garlic (Allium sativum L.) Extract

2021 ◽  
pp. 63-67
Author(s):  
John Lloyd Bernaldez ◽  
Manuela Cecille G. Vicencio
Keyword(s):  
Antibacterial Activity ◽  
Physical Properties ◽  
Allium Sativum ◽  
The Body ◽  
Phytochemical Screening ◽  
E Coli ◽  
Commercial Soap ◽  
Unpleasant Odor ◽  
Ph Range ◽  
Paper Disc

Allium sativum, commonly known as ‘garlic’ has long been used as an antibacterial agent that can actually inhibits growth of infectious agents and at the same time protect the body from the pathogens. This study was conducted to determine the antibacterial activity of soap from garlic extract using the paper-disc method and Kirby-Bauer antibacterial sensitivity test against Staphylococcus aureus and Escherichia coli, and to determine the physical properties of garlic soap and the presence of saponin through phytochemical screening. Garlic soap showed antibacterial activity against E. coli and S. aureus. Mean zone of inhibition was numerically higher in plate extract obtained using garlic soap (14.70mm-18mm), compared to commercial soap. Result of phytochemical screening showed the extract from garlic contains saponin, which is an important ingredient for making soap. Physical properties showed unpleasant odor, dirty white color, smooth texture and pH range of 6.5-7.5. Further studies to determine the potential of garlic soap in the other strains of bacteria.

scholarly journals The Assessment of antimicrobial activity of cerumen (earwax) and antibiotics against pathogenic bacteria isolated from ear pus samples

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Iffat Naz
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Maximum Activity ◽  
Prevalence Data ◽  
Culture Techniques ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Test Organisms ◽  
Staphylococcus Spp

The present study is focused on the assessment of the antimicrobial activity of cerumen and antibiotics against bacteria isolated from ear pus samples. Thus, a total of 50 ear pus samples were collected from infected patients using sterile swabs and were screened using pure culture techniques. Total of 04 different bacterial isolates were identified while, the prevalence data revealed that Pseudomonas spp., were dominant (58%, n = 29) among isolated bacteria followed by Staphylococcus spp., (22%, n = 11), Escherichia coli (14%, n = 7) and Proteus spp., (6%, n = 3). Further, bioassay revealed that Pseudomonas spp., and Staphylococcus spp., were most sensitive to Clindamycin (94.73%) while displayed resistant to Ciprofloxacin and Ampicillin. Similarly, E. coli and Proteus spp., were most sensitive to Ciprofloxacin (92.8-95.21%) as compared to the other antibiotics. Moreover, antibacterial activity of cerumen was also assessed against test organisms and its maximum activity was observed against Pseudomonas spp., (90% equivalent to Clindamycin potency) and Staphylococcus spp., (60% equivalent to Amoxicillin potency) while least effective against E. coli (36%) and Proteus spp., (22%). Thus, it was concluded that the antibacterial activity of cerumen might be due to the presence of potential chemicals i.e. flavonoids and terpenoids.

scholarly journals Features of psychosomatization of medical professionals with occupational deformations

2020 ◽  
Vol 64 (5) ◽  
pp. 278-286
Author(s):  
Olga B. Polyakova ◽  
Tatyana I. Bonkalo
Keyword(s):  
Cardiovascular System ◽  
Statistical Processing ◽  
Maslach Burnout Inventory ◽  
The Body ◽  
Average Level ◽  
Occupational Activity ◽  
Skin Reactions ◽  
Correlation Criterion ◽  
High Level ◽  
Foreign Doctors

Introduction. The stressful conditions of the occupational activity of medical workers lead to the appearance of symptoms of occupational deformations and psychosomatization in them. Purpose. The purpose of identifying the features of medical workers with occupational deformations was achieved by interviewing 6830 medical workers, including 816 cases with pronounced deformations of a professional plan, and establishing links between the components of occupational deformations and psychosomatization. Material and methods. There were used Maslach Burnout Inventory and its modification, Mental Burnout Definition Questionnaire, Questionnaire for Identifying Problems and Their Impact on Health, Psychosomatics of Our Lives, Giessen somatic complaint questionnaire. Mathematical and statistical processing included descriptive statistics, C. Pearson’s correlation criterion, and Cheddock’s table. Results. Medical workers with a high level of exhaustion of emotional and psychoemotional species have a higher average level with a tendency to a high level of deformations of the occupational occupational plan, a higher average level of reduction changes in personal and professional achievements and motivation professional species and an average level of depersonalization processes and estrangement of personal and professional nature dominates exhaustion emotional and psychoemotional species, which determines a high level of complaints about the activity of the cardiovascular system (heart complaints) and intensity of complaints, above average complaints about the activity of the digestive and respiratory systems, gastric complaints, the average level of complaints about the activity of the musculoskeletal system, complaints negative skin reactions, pain in various parts of the body. Discussion. The results of studies by domestic and foreign doctors and psychologists confirm the need for diagnostics, prevention, and correction of professional deformation and leveling of psychosomatic symptoms of medical workers in the context of health care reform. Conclusion. Psychosomatization of medical workers with professional deformations develops according to the coronary type, which gives reason to recommend to medical workers systematic diagnosis, prevention, and treatment of disorders of the cardiovascular system.

scholarly journals Antibacterial and Antifungal Activity of the Human Endometrial Fluid during the Natural Cycle

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Marta Bregón-Villahoz ◽  
Maria-Dolores Moragues ◽  
Inés Arrieta-Aguirre ◽  
Mikel Azkargorta ◽  
Lucía Lainz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Activity Patterns ◽  
Antimicrobial Effect ◽  
The Other ◽  
Natural Cycle ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Related Pattern ◽  
Embryo Transfer Catheter

Purpose. Some microbiota patterns have been associated with favorable IVF prognosis and others with pathological conditions. The endometrial fluid aspirate (EFA) contains antibacterial proteins that are enriched in implantative IVF cycles, but the antimicrobial effect of EFA has not been addressed. We aimed to evaluate the antimicrobial activity of the human endometrial fluid during the natural cycle. Methods. EFA was obtained through an embryo transfer catheter in 38 women, aged 18-40 years, with regular cycles attending to a fertility clinic. The antimicrobial activity of EFAs was tested against two strains of Staphylococcus aureus; one strain each of Streptococcus agalactiae, Enterococcus faecalis, Escherichia coli, and Klebsiella pneumoniae; and three yeasts (Candida albicans, Candida glabrata, and Candida krusei). Results. All samples exhibited antibacterial activity against S. aureus. In addition, 32.4% of EFAs were active against one of the other microorganisms assayed, 16.2% against two, and 5.4% against four of them. In contrast, none exhibited antibacterial activity against E. coli or K. pneumoniae. The antimicrobial activity differs considerably between EFA samples, and we failed to observe a cycle-related pattern. Conclusions. EFA presented two antimicrobial activity patterns: (a) one common to all the samples, exhibiting activity against S. aureus and lack of activity against E. coli and K. pneumoniae, and (b) an individualized pattern, showing activity against some of the other microorganisms tested. The intensity of antibacterial activity differs between EFA samples. Our data suggest that the uterine microbiota is controlled by means of endometrial fluid components.

scholarly journals Effect of intramolecular disulfide bond of bovine lactoferricin on its molecular structure and antibacterial activity against Trueperella pyogenes separated from cow milk with mastitis

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jie Pei ◽  
Lin Xiong ◽  
Min Chu ◽  
Xian Guo ◽  
Ping Yan
Keyword(s):  
Molecular Structure ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Secondary Structure ◽  
Disulfide Bond ◽  
Random Coil ◽  
Cow Milk ◽  
E Coli ◽  
Trueperella Pyogenes ◽  
Intramolecular Disulfide Bond

Abstract Background Lactoferricin (Lfcin) is an antimicrobial activity center of lactoferrin, produced by hydrolysis from the N-terminal of lactoferrin. It was hypothesized that the intramolecular disulfide bond in Lfcin could affect its antibacterial function through influencing its molecular structure. To prove this hypothesis, bovine Lfcin (bLfcin) and its two derivatives, bLfcin with an intramolecular disulfate bond (bLfcin DB) and bLfcin with a mutation C36G (bLfcin C36G), were synthesized, purified, and identified. The circular dichroism spectra of the peptides were detected in solutions with different ionic and hydrophobic strength. The antibacterial activity of the peptides against Trueperella pyogenes, separated from cow milk with mastitis, were determined. Results The secondary structure of bLfcin DB showed more β-turn and less random coil than the other peptides in H2O, similar ratios of secondary structures with bLfcin and bLfcin C36G under ionic conditions, and close percentages of secondary structure with bLfcin under hydrophobic conditions. The synthetic peptides exhibited strong antimicrobial activity against T. pyogenes isolates, T. pyogenes ATCC 19,411, and E. coli ATCC 25,922. The antimicrobial activities of the three peptides were greater against T. pyogenes than against E. coli, and bLfcin DB exhibited higher antibacterial activity compared with its derivatives. Conclusions The intramolecular disulfide bond could change the molecular structure of bLfcin under alternative ionic strengths and hydrophobic effects, and the formation of the disulfide bond is beneficial to executing the antibacterial function of bLfcin.

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