Potential effects of Trachyspermum copticum essential oil and propolis alcoholic extract on Mep3 gene expression of Microsporum canis isolates

Bulbine abyssinica is widely used in folk medicine in Eastern Cape Province, South Africa. The aim of this study was to evaluate the antifungal and toxicity potentials of essential oil, acetone and aqueous extracts of this species using standard procedures and brine shrimp test, respectively. The results showed that the species was active against the growth of Microsporum canis, Microsporum gypseum and Trichophyton rubrum among the evaluated opportunistic fungi. The toxicity results showed that the lowest cysts hatching success was observed with the essential oil, then acetone extract, with aqueous extract exhibiting the highest hatching success. Based on the criterion of toxicity indices of the lethality test, all the plant fractions exhibited LD50 values greater than 1 mg/mL hence are non-toxic. These findings indicate that B. abyssinica is a good source of antifungal agents.

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The Comparative Effect of Hydro Alcoholic and Hydro Distillation Extracts of Melissa officinalis on Acne and Pimple

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.12.35 ◽

2019 ◽

Vol 12 ◽

pp. 35-43 ◽

Cited By ~ 2

Author(s):

Giti Sabet Teymouri ◽

Mozhgan Sabet Teimouri

Keyword(s):

Essential Oil ◽

Rosmarinic Acid ◽

Dry Matter ◽

Water Extract ◽

Distilled Water ◽

Alcoholic Extract ◽

Melissa Officinalis ◽

Sebaceous Glands ◽

Comparative Effect ◽

The People

One of the famous plants in family Lamiaceae isMelissa officinaliswhich is one of the important herbs for health and Medicine. Melisa contains Rosmarinic acid, flavonoids and phenolic. These components have tightening and rejuvenation effect on skin. Also, it has monoterpenes and di-terpenes which are antibacterial. Essential oil of Melisa forms a good complex with sebum, so it can decrease inflammation and acne pain 30 gr of dry leaves and stems of Melisa in 500 ml of distilled water were used for hydro-distillation. For hydro alcoholic extract, we used 150 gr of dry matter (leaves and stems) in 500 ml of ethyl alcohol at 25 °C for 10 days. Then, the alcoholic extract was diluted 5 times with water 20 persons were under experiment for 3 months. Extracts were sprayed on skin 3-5 times a day. The results showed that all the people treated got the best result of this treatment. After the application of the water extract of Melissa, we observed 75% of clients improved the skin pore, 65% of clients improved neurological problems and Acne infections and 92% recovery of hyper secretion of Sebaceous glands. We observed the same results with Hydro Alcoholic extracts. 75% recovery of clients with improved skin pore, 95% of clients with improved neurological problems and Acne infections and 90% recovery of hyper secretion of Sebaceous glands. The results showed that Melisa extract has value of applying on some skin problems.

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Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia

PeerJ ◽

10.7717/peerj.11098 ◽

2021 ◽

Vol 9 ◽

pp. e11098

Author(s):

Lixia Sheng ◽

Shu Zang ◽

Jianwen Wang ◽

Tiantian Wei ◽

Yong Xu ◽

...

Keyword(s):

Gene Expression ◽

Essential Oil ◽

Gene Expression Pattern ◽

Mass Spectrometry Analysis ◽

High Yield ◽

Rosa Rugosa ◽

Spectrometry Analysis ◽

Component Content ◽

China Rose ◽

Aroma Components

Rosa rugosa is an important natural perfume plant in China. Rose essential oil is known as ‘liquid gold’ and has high economic and health values. Monoterpenes are the main fragrant components of R. rugosa flower and essential oil. In this study, a member of the hydrolase gene family RrNUDX1 was cloned from Chinese traditional R. rugosa ‘Tang Hong’. Combined analysis of RrNUDX1 gene expression and the aroma components in different development stages and different parts of flower organ, we found that the main aroma component content was consistent with the gene expression pattern. The RrNUDX1 overexpressed Petunia hybrida was acquired via Agrobacterium-mediated genetic transformation systems. The blades of the transgenic petunias became wider and its growth vigor became strong with stronger fragrance. Gas chromatography with mass spectrometry analysis showed that the contents of the main aroma components of the transgenic petunias including methyl benzoate significantly increased. These findings indicate that the RrNUDX1 gene plays a role in enhancing the fragrance of petunia flowers, and they could lay an important foundation for the homeotic transformation of RrNUDX1 in R. rugosa for cultivating new R. rugosa varieties of high-yield and -quality essential oil.

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Involvement of HPI-axis in anesthesia with Lippia alba essential oil citral and linalool chemotypes: gene expression in the secondary responses in silver catfish

Fish Physiology and Biochemistry ◽

10.1007/s10695-018-0548-3 ◽

2018 ◽

Vol 45 (1) ◽

pp. 155-166 ◽

Cited By ~ 10

Author(s):

Carine de Freitas Souza ◽

Sharine Descovi ◽

Matheus Dellaméa Baldissera ◽

Kalyne Bertolin ◽

Adriane Erbice Bianchini ◽

...

Keyword(s):

Gene Expression ◽

Essential Oil ◽

Silver Catfish ◽

Lippia Alba ◽

Hpi Axis

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Development, Characterization, and Evaluation of Novel Broad-Spectrum Antimicrobial Topical Formulations from Cymbopogon martini (Roxb.) W. Watson Essential Oil

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/9812093 ◽

2018 ◽

Vol 2018 ◽

pp. 1-16 ◽

Cited By ~ 3

Author(s):

Negero Gemeda ◽

Ashenif Tadele ◽

Hirut Lemma ◽

Biruktawit Girma ◽

Getachew Addis ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Broad Spectrum ◽

Chemical Constituents ◽

Trichophyton Mentagrophytes ◽

Microsporum Canis ◽

Skin Infections ◽

Topical Formulations ◽

Life Study

Background. Skin infections were the most frequently encountered of all infections and the 4th leading cause of nonfatal disease burden. Topical drugs have been used for the management of skin infections. The growing concern of drug resistance to the topical agents has warned the need for continuous development of novel drug. Essential oils are the best candidate for new drug with different mode of action and target as they are rich in chemical constituents. Objective. To evaluate and develop safe and effective topical antimicrobial formulations from essential oil of Cymbopogon martini. Method. Essential oil was extracted using hydrodistillation aerial part C. martini and topical formulations were prepared in five different semisolid bases. In vitro antimicrobial investigations were performed on essential oil and topical formulations. Skin sensitizations of the formulations were evaluated using guinea pig maximization. Results. The essential oil of C. martini has shown broad-spectrum antimicrobial potency against all tested organisms with MIC value ranging from 0.65 to 10 μg/ml. Absolute inhibitions of growth of fungi were observed against Trichophyton mentagrophytes and Trichophyton rubrum at concentrations above 1% of oil and against Microsporum canis and Trichophyton verrucosum at a concentration of 4% oil. Among topical formulations, the highest antimicrobial activity was recorded in hydrophilic ointment followed by macrogol blend ointment. The antimicrobial activity of oil was higher in fungal pathogen compared to bacteria. Gram positive bacteria were more sensitive than gram negative bacteria. Hydrophilic and macrogol blend ointment containing 5% oil did not produce any skin sensitization on guinea pigs. Conclusion. In conclusion, topical formulations of C. martini essential oil can be alternative topical agents with safe broad-spectrum activity for the treatment of skin disorder. Further studies should focus on shelf life study and clinical study of the product.

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Effects of Zataria multiflora Boiss. essential oil on growth and gene expression of enterotoxins A, C and E in Staphylococcus aureus ATCC 29213

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2013.02.020 ◽

2013 ◽

Vol 163 (2-3) ◽

pp. 159-165 ◽

Cited By ~ 39

Author(s):

Maryam Azizkhani ◽

Ali Misaghi ◽

Afshin Akhondzadeh Basti ◽

Hassan Gandomi ◽

Hedayat Hosseini

Keyword(s):

Gene Expression ◽

Staphylococcus Aureus ◽

Essential Oil ◽

Zataria Multiflora

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The Chemical Composition and Anti-mycobacterial Activities of Trachyspermum copticum and Pelargonium graveolens Essential Oils

Recent Patents on Anti-Infective Drug Discovery ◽

10.2174/1574891x14666191028113321 ◽

2020 ◽

Vol 15 (1) ◽

pp. 68-74 ◽

Cited By ~ 1

Author(s):

Jalil Kardan-Yamchi ◽

Mohaddese Mahboubi ◽

Hossein Kazemian ◽

Gholamreza Hamzelou ◽

Mohammad M. Feizabadi

Keyword(s):

Mycobacterium Tuberculosis ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Concentration ◽

Mycobacterium Fortuitum ◽

Drug Resistant ◽

Pelargonium Graveolens ◽

Trachyspermum Copticum

Background: Microbial resistance to antibiotics and their adverse effects related to these antibiotics are a matter of global public health in the 21th century. The emergence of drug-resistant strains, has gained the interest of the scientists to discover new antimicrobial agents from the essential oil of medicinal plants. Methods: Anti-mycobacterial effects of Trachyspermum copticum and Pelargonium graveolens essential oils were determined against multi-drug resistant clinical strains of Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium fortuitum and standard strain of Mycobacterium tuberculosis H37Rv by a Broth micro-dilution method. Pelargonium graveolens plant named Narmada was discovered by Kulkarni R.N et al. (Patent ID, USPP12425P2) and a formulation comprising thymol obtained from Trachyspermum is useful in the treatment of drug-resistant bacterial infections (Patent ID, US6824795B2). The chemical composition of hydro-distilled essential oils was determined by GC and GC-MS. Results: Minimum Inhibitory Concentration (MIC) values for T. copticum essential oil against tested isolates were ranged from 19.5 µg/mL to 78 µg/mL. The least minimum inhibitory concentration of P. graveolens extract against M. Kansasii and MDR-TB was 78 µg/ml. Conclusion: The results of the present research introduced T. copticum and P. graveolens essential oils as a remarkable natural anti-mycobacterial agent, but more pharmacological studies are required to evaluate their efficacy in animal models.

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Lipid-Lowering Effects of Lotus Leaf Alcoholic Extract on Serum, Hepatopancreas, and Muscle of Juvenile Grass Carp via Gene Expression

Frontiers in Physiology ◽

10.3389/fphys.2020.584782 ◽

2020 ◽

Vol 11 ◽

Author(s):

Junpeng Yao ◽

Pengcheng Hu ◽

Yanhong Zhu ◽

Yingyan Xu ◽

Qingsong Tan ◽

...

Keyword(s):

Gene Expression ◽

Fatty Acid ◽

Lipid Metabolism ◽

Lipid Droplet ◽

Grass Carp ◽

Lipid Lowering ◽

Lipid Deposition ◽

Alcoholic Extract ◽

Mrna Levels ◽

Lotus Leaf

Compared with wild grass carp (Ctenopharyngodon idellus), intensively cultured fish displayed disordered lipid metabolism, showing excess lipid deposition in the hepatopancreas and muscle. Lotus leaf prevents fat accumulation in humans and may have similar effects on fish. This study explored the regulatory mechanisms by which the dietary addition of an alcoholic extract of lotus leaf (AELL) reduced lipid deposition in the hepatopancreas and muscle of juvenile grass carp. The fish (average initial weight: 34.00 ± 0.40 g) were fed four experimental diets containing different AELL levels (0, 0.07, 0.14, and 0.21%) for 8 weeks. Serum components, lipid droplet size, triacylglycerol (TAG) content, enzymatic activities, and mRNA levels of genes related to lipid metabolism in the hepatopancreas and muscle were analyzed. The results show that dietary AELL supplementation significantly reduced the TAG content and lipid droplet area in the histological sections as well as the fatty acid synthase (FAS) activity in both the hepatopancreas and muscle but enhanced the activities of lipoprotein lipase (LPL) and carnitine palmitoyltransferase I (CPT1) in both tissues. In addition, dietary AELL supplementation decreased the mRNA expression of genes involved in fatty acid uptake (cd36, fatp1/fatp4/fatp6, fabp10/fabp11, acsl1/acsl4) and de novo lipid synthesis (pgd, g6pd, and fasn) as well as the transcription factors pparg and srebf1 in the hepatopancreas and muscle but increased the mRNA levels of genes relating to lipid catabolism (cpt1a, lipe, pnpla2, lpl), lipid transportation (apob), and the transcription factor ppara in both tissues. In conclusion, dietary AELL supplementation reduced lipid accumulation in the hepatopancreas and muscle by affecting the gene expression of proteins with known effects on lipid metabolism in juvenile grass carp.

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Modulation ofmecA Gene Expression by Essential Oil fromSalvia sclareaand Synergism with Oxacillin in Methicillin ResistantStaphylococcus epidermidisCarrying Different Types of Staphylococcal Chromosomal Cassettemec

International Journal of Microbiology ◽

10.1155/2016/6475837 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Romana Chovanová ◽

Mária Mikulášová ◽

Štefánia Vaverková

Keyword(s):

Gene Expression ◽

Essential Oil ◽

Synergistic Effect ◽

Real Time Pcr ◽

Staphylococcus Epidermidis ◽

Salvia Sclarea ◽

Resistant Genes ◽

Different Types ◽

Checkerboard Assay ◽

Time Kill

The essential oil (EO) fromSalvia sclareawas shown to increase the susceptibility of methicillin resistantStaphylococcus epidermidis(MRSE) isolates to oxacillin. The purpose of this study was to investigate the effect of EO fromS. sclareaon expression ofmecA gene of MRSE carrying different types of staphylococcal chromosomal cassette (SCCmec) and to evaluate potential synergistic effect of EO with oxacillin. Using real-time PCR we found that EO alone inhibited the expression of the resistant genesmecA,mecR1, andmecI andblaZ,blaR1, andblaI. The use of the combination of EO with oxacillin resulted in significantly inhibited expression ofmecA gene in all tested strains with different types of SCCmec. Using time-kill assay and checkerboard assay we confirmed synergistic effect of EO fromS. sclareaand oxacillin in MRSE.

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