scholarly journals Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts

2018 ◽  
Vol 14 (2) ◽  
pp. 601-607 ◽  
Author(s):  
Valerie Aurore ◽  
Fabienne Caldana ◽  
Marianne Blanchard ◽  
Solange Kharoubi Hess ◽  
Nils Lannes ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Activity ◽  
Bacterial Pathogens ◽  
Human Osteoclasts

Synergistic Antibacterial Efficacy of Biogenic Synthesized Silver Nanoparticles using Ajuga bractosa with Standard Antibiotics: A Study Against Bacterial Pathogens

2020 ◽  
Vol 21 (3) ◽  
pp. 206-218 ◽  
Author(s):  
Sadia Nazer ◽  
Saiqa Andleeb ◽  
Shaukat Ali ◽  
Nazia Gulzar ◽  
Tariq Iqbal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bacterial Pathogens ◽  
Potential Effect ◽  
Innovative Technology ◽  
Multi Drug Resistance ◽  
Phytochemical Screening ◽  
Cell Proliferation Inhibition ◽  
Agar Well Diffusion Assay ◽  
Scanning Electron

Background: Multi-drug resistance in bacterial pathogens is a major concern of today. Green synthesis technology is being used to cure infectious diseases. Objectives: The aim of the current research was to analyze the antibacterial, antioxidant, and phytochemical screening of green synthesized silver nanoparticles using Ajuga bracteosa. Methods: Extract of A. bracteosa was prepared by maceration technique. Silver nanoparticles were synthesized using A. bracteosa extract and were confirmed by UV-Vis spectrophotometer, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, TLC-Bioautography, TLC-Spot screening, antioxidant, and phytochemical screening were also investigated. Results: UV-Vis spectrum and Scanning electron microscopy confirmed the synthesis of green nanoparticles at 400 nm with tube-like structures. FTIR spectrum showed that functional groups of nanoparticles have a role in capping and stability of AgNP. Agar well diffusion assay represented the maximum antibacterial effect of ABAgNPs against Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa at 0.10 g/mL concentration compared to ABaqu. Two types of interactions among nanoparticles, aqueous extract, and antibiotics (Synergistic and additive) were recorded against tested pathogens. Crystal violet, MTT, TLC-bio-autography, and spot screening supported the findings of the antibacterial assay. Highest antioxidant potential effect in ABaqu was 14.62% (DPPH) and 13.64% (ABTS) while 4.85% (DPPH) and 4.86% (ABTS) was recorded in ABAgNPs. Presence of phytochemical constituents showed pharmacological importance. Conclusion: It was concluded that green synthesis is an innovative technology in which natural products are conjugated with metallic particles and are used against infectious pathogens. The current research showed the significant use of green nanoparticles against etiological agents.

scholarly journals A Fish Galectin-8 Possesses Direct Bactericidal Activity

2020 ◽  
Vol 22 (1) ◽  
pp. 376
Author(s):  
Tengfei Zhang ◽  
Shuai Jiang ◽  
Li Sun
Keyword(s):  
Bactericidal Activity ◽  
Bacterial Pathogens ◽  
Bactericidal Effect ◽  
Immune Defense ◽  
Carbohydrate Binding ◽  
Dependent Manner ◽  
Animal Cells ◽  
Gram Negative ◽  
Tongue Sole ◽  
Wide Range

Galectins are a family of animal lectins with high affinity for β-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a tandem repeat galectin-8 from tongue sole Cynoglossus semilaevis (designated CsGal-8). CsGal-8 possesses conserved carbohydrate recognition domains (CRDs), as well as the conserved HXNPR and WGXEE motifs that are critical for carbohydrate binding. CsGal-8 was constitutively expressed in nine tissues of tongue sole and up-regulated in kidney, spleen, and blood by bacterial challenge. When expressed in HeLa cells, CsGal-8 protein was detected both in the cytoplasm and in the micro-vesicles secreted from the cells. Recombinant CsGal-8 (rCsGal-8) bound to lactose and other carbohydrates in a dose dependent manner. rCsGal-8 bound to a wide range of gram-positive and gram-negative bacteria and was co-localized with the bound bacteria in animal cells. Lactose, fructose, galactose, and trehalose effectively blocked the interactions between rCsGal-8 and different bacteria. Furthermore, rCsGal-8 exerted potent bactericidal activity against some gram-negative bacterial pathogens by directly damaging the membrane and structure of the pathogens. Taken together, these results indicate that CsGal-8 likely plays an important role in the immune defense against some bacterial pathogens by direct bacterial interaction and killing.

Spermine increases bactericidal activity of silver-nanoparticles against clinical methicillin-resistant Staphylococcus aureus

2018 ◽  
Vol 29 (12) ◽  
pp. 1824-1828 ◽  
Author(s):  
Chang Liu ◽  
Han Shen ◽  
Su Wang ◽  
Xiaoli Cao ◽  
Hongpan Xu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Bactericidal Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

Green synthesis of silver nanoparticles using Carum copticum: Assessment of its quorum sensing and biofilm inhibitory potential against gram negative bacterial pathogens

2020 ◽  
Vol 144 ◽  
pp. 104172 ◽  
Author(s):  
Faizan Abul Qais ◽  
Anam Shafiq ◽  
Iqbal Ahmad ◽  
Fohad Mabood Husain ◽  
Rais Ahmad Khan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Quorum Sensing ◽  
Green Synthesis ◽  
Bacterial Pathogens ◽  
Gram Negative ◽  
Carum Copticum ◽  
Inhibitory Potential

Understanding the potential of bio-fabricated non-oxidative silver nanoparticles to eradicate Leishmania and plant bacterial pathogens

2020 ◽  
Vol 10 (6) ◽  
pp. 2057-2067 ◽  
Author(s):  
Bilal Javed ◽  
Naveed Iqbal Raja ◽  
Akhtar Nadhman ◽  
Zia-ur-Rehman Mashwani
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Pathogens

Green synthesis and physical properties of Gum Arabic‐silver nanoparticles and its antibacterial efficacy against fish bacterial pathogens

2020 ◽  
Author(s):  
Mahmoud M. El‐Adawy ◽  
Alaa Eldin Eissa ◽  
Mohamed Shaalan ◽  
Ahmed A. Ahmed ◽  
Nehal A. Younis ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Physical Properties ◽  
Green Synthesis ◽  
Bacterial Pathogens ◽  
Gum Arabic ◽  
Antibacterial Efficacy

scholarly journals Levan-Capped Silver Nanoparticles for Bactericidal Formulations: Release and Activity Modelling

2019 ◽  
Vol 20 (6) ◽  
pp. 1502 ◽  
Author(s):  
Álvaro González-Garcinuño ◽  
Rubén Masa ◽  
María Hernández ◽  
Ángel Domínguez ◽  
Antonio Tabernero ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Dose Response ◽  
Bactericidal Activity ◽  
Lethal Dose ◽  
Response Model ◽  
Inhibition Activity ◽  
E Coli ◽  
Unique Model ◽  
Bacteria Inhibition ◽  
Silver Release

An environmentally friendly technique was used to produce levan-capped silver nanoparticles of about 30 nm (with a loading of 30%) that showed bactericide effect, for E. coli and B. subtilis. That effect was mathematically studied with a dose-response model (lethal dose of 12.4 ppm and 6.8 ppm respectively). These silver nanoparticles were subsequently introduced in a gel to create a silver release system with bacteria inhibition activity. Silver release from the gel and its bactericidal activity was theoretically studied to develop a unique model that is able to predict accurately both silver release and lethal dose for any type of bacteria. This model will be useful for performing predictions for future silver in gel applications.

A new method for producing highly concentrated non-aqueous dispersions of silver nanoparticles and the evaluation of their bactericidal activity

2014 ◽  
Vol 16 (11) ◽  
Author(s):  
Roselaine da S. Oliveira ◽  
Marcos A. Bizeto ◽  
Ana M. A. Liberatore ◽  
Ivan H. J. Koh ◽  
Fernanda F. Camilo
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Activity ◽  
New Method ◽  
Aqueous Dispersions
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