scholarly journals Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 833 ◽  
Author(s):  
Sanghyeon Oh ◽  
Young Joo Kim ◽  
Eun Kyoung Lee ◽  
Sung Wook Park ◽  
Hyeong Gon Yu
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Cell Viability ◽  
Lethal Dose ◽  
Ultraviolet B ◽  
Stress Model ◽  
Antioxidative Effect ◽  
Pathogenic Factors ◽  
Intracellular Ros ◽  
Antioxidative Effects

Oxidative stress has been implicated as critical pathogenic factors contributing to the etiology of diabetic retinopathy and other retinal diseases. This study investigated antioxidative effect of ascorbic acid and astaxanthin on ARPE-19 cells within an oxidative stress model induced by common biological sources of reactive oxygen species (ROS). Hydrogen peroxide (H2O2) at concentrations of 0.1–0.8 mM and 20–100 mJ/cm2 of ultraviolet B (UVB) were treated to ARPE-19 cells. Cell viability and intracellular ROS level changes were measured. With the sublethal and lethal dose of each inducers, 0–750 μM of ascorbic acid and 0–40 μM of astaxanthin were treated to examine antioxidative effect on the model. Ascorbic acid at concentrations of 500 and 750 μM increased the cell viability not only in the UVB model but also in the H2O2 model, but 20 and 40 μM of astaxanthin only did so in the UVB model. The combination of ascorbic acid and astaxanthin showed better antioxidative effect compared to each drug alone, suggesting a synergistic effect.

Evaluation of oxidative stress and its modulation by L-Arginine and L-Ascorbic acid in repetitive restraint stress model in Wistar rats

2020 ◽  
Vol 17 ◽  
pp. 100172
Author(s):  
Giridhari Pal ◽  
Tapan Behl ◽  
Vishwajeet Rohil ◽  
Mimansa Khandelwal ◽  
Garima Gupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Stress Model

scholarly journals Optimized models of xenobiotic-induced oxidative stress in HepG2 cells

2021 ◽  
Vol 18 (5) ◽  
pp. 1001-1007
Author(s):  
Yollada Sriset ◽  
Waranya Chatuphonprasert ◽  
Kanokwan Jarukamjorn
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Hepg2 Cells ◽  
Sodium Selenite ◽  
Cellular Injury ◽  
Stress Model ◽  
Total Glutathione ◽  
Tert Butyl Hydroperoxide ◽  
Stress Models

Purpose: To evaluate the molecular impact of ethanol, sodium selenite, and tert-butyl hydroperoxide (TBHP) on oxidant-antioxidant balance in HepG2 cells to establish an optimized oxidative stress model of HepG2 cells. Methods: HepG2 cells were treated with ethanol (10 - 500 mM) and sodium selenite (1 - 10 µM) for 24 and 48 h and with TBHP (50 - 200 µM) for 3 and 24 h, respectively. Biomarkers for cellular injury, ie, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and for antioxidant system, i.e., superoxide dismutase (SOD), catalase (CAT), and total glutathione content, were determined. Results: All treatments increased the levels of LDH, AST, ALT, and MDA but decreased SOD and CAT activities and the total glutathione content in HepG2 cells. Oxidative stress was induced by these oxidative stressors in HepG2 cells via oxidant-antioxidant imbalance, with TBHP (100 µM, 3 h) acting as a powerful oxidant based on the minimal time to induce oxidative stress. The antioxidants, ascorbic acid and gallic acid, improved oxidant-antioxidant imbalance against xenobiotic-induced oxidative stress in HepG2 cells. Conclusion: These oxidative stress models are suitable for investigating the antioxidant and/or hepatoprotective potential of chemicals, including natural compounds.

scholarly journals Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia by Inhibiting the Nox4/ROS Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Jian Shen ◽  
Kaiyuan Huang ◽  
Yu Zhu ◽  
Kangli Xu ◽  
Renya Zhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cerebral Ischemia ◽  
Protein Expression ◽  
Cell Viability ◽  
Tube Formation ◽  
Neurological Function ◽  
Neurological Deficits ◽  
Ros Generation ◽  
Buyang Huanwu Decoction ◽  
Intracellular Ros

Background. Buyang Huanwu decoction (BYHWD), an important traditional Chinese medicine (TCM), has been used clinically for centuries for the treatment of various diseases. The study aims to explore the BYHWD effects on angiogenesis and neuroprotection after cerebral ischemia/reperfusion (CI/R) injury in rats and to explore the underlying angiogenic roles and mechanisms of BYHWD in hydrogen peroxide (H2O2) induced oxidative stress in human umbilical vein endothelial cells (HUVECs) model. Methods. The effects of BYHWD on neurological function were screened by measuring neurological deficits, spatial memory function, and angiogenesis (by microvascular density (MVD) and cerebral blood flow (CBF)) after CI/R injury in middle cerebral artery occlusion (MCAO) in vivo in rats. In vitro, we examined the angiogenic roles and mechanisms of action of BYHWD in an H2O2-induced oxidative stress HUVECs model by measuring cell viability, apoptosis, vascular tube formation, intracellular ROS generation, NADPH oxidase (Nox) activity, and Nox4 protein expression. Results. BYHWD significantly improved neurological function, including neurological deficits and spatial learning and memory, and significantly increased MVD and CBF in the ischemic penumbra after CI/R injury in rats. BYHWD significantly increased cell viability, inhibited apoptosis, induced vascular tube formation, decreased intracellular ROS generation, and reduced Nox activity and Nox4 protein expression in H2O2-treated HUVECs in a dose-dependent manner. Conclusions. Our study demonstrates that BYHWD promotes neurological function recovery and increases angiogenesis. BYHWD exerts angiogenic effects against cerebral ischemic injury through the downregulation of Nox4, which results in the reduction of ROS generation.

scholarly journals Dipeptidyl Peptidase-4 Involved in Regulating Mitochondria Function in Cardiomyocytes through Nrf2 and PGC-1α Signaling

2020 ◽  
Author(s):  
Shih-Yi Lee ◽  
Shao-Tung Wu ◽  
Ming-Jai Su ◽  
Yao-Jen Liang ◽  
Hui-Chun Ku
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Mitochondrial Function ◽  
Dipeptidyl Peptidase ◽  
Atp Production ◽  
Dipeptidyl Peptidase 4 ◽  
Transmembrane Glycoprotein ◽  
Mitochondrial Ros ◽  
Intracellular Ros ◽  
Dependent Pathway

Abstract Background: Oxidative stress is an imbalance between the production of reactive oxygen species (ROS) and the detoxification ability of reactive intermediates. It will lead to mitochondrial damage and dysfunction, resulting in the dysfunction of bioenergetic control and loss of ATP production, which is contributed to the pathogenesis of cardiac diseases. Dipeptidyl peptidase-4 (DPP4) is a transmembrane glycoprotein ubiquitously expressed and has multifunctional properties. DPP4 inhibitors are a class of oral diabetes drugs that inhibit the enzyme activity. In addition to its enzymatic property, considerably less is known regarding the nonenzymatic function of DPP4.Methods: We knocked down DPP4 gene expression in cultured cardiomyocytes to exclude any external and enzymatic substrate effects and compared the response between DPP4 knockdown and wild-type cardiomyocytes in response to oxidative stress.Results: H2O2-induced oxidative stress-stimulated intracellular and mitochondrial ROS concentration led to the loss of mitochondrial function, ATP production, and increased Bax and cleaved PARP expression, resulting in the loss of cell viability in cardiomyocytes. Oxidative stress induced DPP4 expression. Knocking down DPP4 ameliorated H2O2-induced loss of cell viability by preserving mitochondrial bioenergy, reducing intracellular ROS production, alleviating apoptosis-associated protein expression. Knocking down DPP4 increased its capability against oxidative stress by enhancing Nrf2 and PGC-1α signaling, which is associated with preserving mitochondrial function.Conclusions: DPP4 is a mediator of oxidative stress. Knocking down DPP4 without any external substrate mediators increased the capability of cardiomyocytes against oxidative stress, which indicated that DPP4 mediated more than the enzymatic-dependent pathway.

scholarly journals Assessment of Lemon Balm (Melissa officinalisL.) Hydrogels: Quality and Bioactivity in Skin Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Kristina Ramanauskienė ◽  
Ada Stelmakiene ◽  
Daiva Majienė
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Rosmarinic Acid ◽  
Stress Conditions ◽  
Ros Generation ◽  
Lemon Balm ◽  
Skin Cells ◽  
Biological Studies ◽  
Intracellular Ros

Theaimof the study was to design gels with lemon balm extract, assess their quality, and investigate the effect of rosmarinic acid on skin cells in normal conditions and under oxidative stress.Methods. The quantities of rosmarinic acid (RA) released from gels were evaluated by applying the HPLC technique. HaCaT cell viability was assessed by using the MTT method. ROS generation was measured using DCFH-DA dye. Theresultsshowed that the gelling material affected the release of RA content from gels. Lower and slower RA content release was determined in carbomer-based gels. After 6 hours of biopharmaceutical researchin vitro, at least 4% of RA was released from the gel. The results of the biological studies on HaCaT cells demonstrated that, in the oxidative stress conditions, RA reduced intracellular ROS amounts to 28%; 0.25–0.5 mg/mL of RA increased cell viability by 10–24% and protected cells from the damage caused by H2O2.Conclusions. According to research results, it is appropriate to use a carbomer as the main gelling material, and its concentration should not exceed 1.0%. RA, depending on the concentration, reduces the amount of intracellular ROS and enhances cell viability in human keratinocytes in oxidative stress conditions.

scholarly journals Phenolic Content, Antioxidant and Astroprotective Response to Oxidative Stress of Ethanolic Extracts of Mentha longifolia from Sinai

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Tarek F. Eissa ◽  
Elena González-Burgos ◽  
M. Emilia Carretero ◽  
M. Pilar Gómez-Serranillos
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Phenolic Content ◽  
Water Soluble ◽  
Herbal Remedies ◽  
Total Phenolic ◽  
Aerial Parts ◽  
Intracellular Ros ◽  
Ethanolic Extracts ◽  
Mentha Longifolia

The aerial parts of Mentha longifolia L. are used as herbal remedies for curing different diseases through traditional Bedouin medicine. The antioxidant activity of the ethanolic extracts of M. longifolia was investigated measuring peroxyl radical-scavenging activity by ORAC assay, with Trolox (a water-soluble analogue of α-tocopherol) employed as reference compound. In addition, the total content of phenolic compounds estimated by the Folin-Ciocalteau method and the identification of the polyphenols using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) have been performed. Furthermore, the effect of these extracts on cell viability and intracellular ROS production was assayed using the U373-MG human astrocytoma cell line in a H2O2-induced oxidative stress model. Results showed that the major type of polyphenols found were benzoic acids, cinnamic acids, flavones and flavanones. The total phenolic content was 37.7 mg gallic acid/g sample and the ORAC value was 1.355 μmol TE/mg sample. The data obtained in cellular assays demonstrated that these ethanolic extracts protected H2O2-induced astrocyte damage by increasing cell viability and inhibiting production of intracellular ROS. These results suggest that the investigated extracts obtained from the aerial parts of M. longifolia have antioxidant potential related to their phenol content which have important beneficial health effects, especially in those disease associated with ROS.

scholarly journals Carbon Monoxide Partially Mediates Protective Effect of Resveratrol Against UVB-Induced Oxidative Stress in Human Keratinocytes

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 432 ◽  
Author(s):  
Janice N. Averilla ◽  
Jisun Oh ◽  
Jong-Sang Kim
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Radical Scavenging ◽  
Protoporphyrin Ix ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Related Factor ◽  
Uvb Radiation ◽  
Intracellular Ros ◽  
Tin Protoporphyrin

Based on the antioxidative effect of resveratrol (RES) in mitigating reactive oxygen species (ROS) production through the induction of nuclear factor-erythroid 2-related factor-2 (Nrf2)/heme oxigenase-1 (HO-1) signaling pathway, we investigated whether the protective activity of RES against ROS-mediated cytotoxicity is mediated by intracellular carbon monoxide (CO), a product of HO-1 activity, in ultraviolet B (UVB)-irradiated human keratinocyte HaCaT cells. The cells were exposed to UVB radiation following treatment with RES and/or CO-releasing molecule-2 (CORM-2). RES and/or CORM-2 upregulated HO-1 protein expression, accompanied by a gradual reduction of UVB-induced intracellular ROS levels. CORM-2 reduced intracellular ROS in the presence of tin protoporphyrin IX, an HO-1 inhibitor, indicating that the cytoprotection observed was mediated by intracellular CO and not by HO-1 itself. Moreover, CORM-2 decreased RES-stimulated mitochondrial quantity and respiration and increased the cytosolic protein expressions of radical-scavenging superoxide dismutases, SOD1 and SOD2. Taken together, our observations suggest that RES and intracellular CO act independently, at least partly, in attenuating cellular oxidative stress by promoting antioxidant enzyme expressions and inhibiting mitochondrial respiration in UVB-exposed keratinocytes.

Mitigation of arsenic-mediated renal oxidative stress in rat by Pleurotus florida lectin

2010 ◽  
Vol 30 (8) ◽  
pp. 940-951 ◽  
Author(s):  
Asit Kumar Bera ◽  
Tanmoy Rana ◽  
Subhashree Das ◽  
Debasis Bhattacharya ◽  
Diganta Pan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
No Production ◽  
Dependent Manner ◽  
Oral Supplementation ◽  
Pleurotus Florida ◽  
Antioxidative Effects ◽  
Mushroom Lectin

Oyster mushroom, Pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. Here, the study aimed to investigate the antioxidative effects of mushroom (Pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. Animals were divided into four groups; Group 1 was control. Groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus oral supplementation of mushroom lectin (150 mg/kg body weight) respectively. Both ascorbic acid and mushroom lectin prevented the arsenic-mediated growth retardation and normalized the elevated kidney weight. Disrupted activities of superoxide dismutase (SOD) and catalase (CAT) and enhanced lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxides (NO) production in kidney caused by arsenic could also be maintained towards normalcy by supplementation of mushroom lectin and ascorbic acid. These antioxidative effects were exhibited in a time-dependant manner. Further, arsenic-mediated down-regulation of messenger RNA (mRNA) expression of superoxide dismutase 2 (SOD2) gene was obstructed by these agents. Thus it was found that mushroom lectin reversed the effect of arsenic-mediated oxidative stress in a time-dependent manner.

Sign in / Sign up

Export Citation Format

Share Document