Plantaricin A synthesized by Lactobacillus plantarum induces in vitro proliferation and migration of human keratinocytes and increases the expression of TGF-β1, FGF7, VEGF-A and IL-8 genes

Peptides ◽  
2011 ◽  
Vol 32 (9) ◽  
pp. 1815-1824 ◽  
Author(s):  
Daniela Pinto ◽  
Barbara Marzani ◽  
Fabio Minervini ◽  
Maria Calasso ◽  
Giammaria Giuliani ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Human Keratinocytes ◽  
In Vitro Proliferation ◽  
And Migration ◽  
Tgf Β1 ◽  
Proliferation And Migration

scholarly journals Achyrocline satureioides (Lam.) DC (Asteraceae) Extract-Loaded Nanoemulsions as a Promising Topical Wound Healing Delivery System: In Vitro Assessments in Human Keratinocytes (HaCaT) and HET-CAM Irritant Potential

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1241
Author(s):  
Lucélia Albarello Balestrin ◽  
Tainá Kreutz ◽  
Flávia Nathiely Silveira Fachel ◽  
Juliana Bidone ◽  
Nicolly Espindola Gelsleichter ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hacat Cell ◽  
Chorioallantoic Membrane ◽  
Human Keratinocytes ◽  
Scratch Assay ◽  
Hen’S Egg ◽  
And Migration ◽  
Achyrocline Satureioides ◽  
Proliferation And Migration

Achyrocline satureioides (Lam.) DC Asteraceae extracts (ASEs) have been investigated for the treatment of various skin disorders. This study reports the effects of ASE-loaded nanoemulsions (NEASE) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen’s egg chorioallantoic membrane test (HET-CAM). NEASE exhibited a polydispersity index above 0.12, with a droplet size of 300 nm, ζ-potential of −40 mV, and content of flavonoids close to 1 mg/mL. No cytotoxicity of the ASE was observed on HaCaT by MTT assay (up to 10 µg/mL). A significant increase of HaCaT viability was observed to NEASE (up to 5 μg/mL of flavonoids), compared to treatment with the ASE. The necrosis death evaluation demonstrated that only NEASE did not lead to cell death at all the tested concentrations. The scratch assay demonstrated that NEASE was able to increase the cell migration at low flavonoid concentrations. Finally, the HET-CAM test proved the non-irritative potential of NEASE. Overall, the results indicate the potential of the proposed formulations for topical use in wound healing, in view of their promising effects on proliferation and migration in keratinocytes, combined with an indication of the absence of cytotoxicity and non-irritating potential.

scholarly journals Multivalent conjugates of basic fibroblast growth factor enhance in vitro proliferation and migration of endothelial cells

2018 ◽  
Vol 6 (5) ◽  
pp. 1076-1083 ◽  
Author(s):  
Aline Zbinden ◽  
Shane Browne ◽  
Eda I. Altiok ◽  
Felicia L. Svedlund ◽  
Wesley M. Jackson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Great Promise ◽  
Fibroblast Growth ◽  
In Vitro Proliferation ◽  
And Migration ◽  
Regenerative Therapies ◽  
Proliferation And Migration

Multivalent growth factor conjugates hold great promise for regenerative therapies.

37 Cellular Iron Drives In Vitro Proliferation and Migration in Colorectal Adenocarcinoma

2008 ◽  
Vol 134 (4) ◽  
pp. A-5
Author(s):  
Matthew J. Brookes ◽  
Keith Roberts ◽  
Tariq Iqbal ◽  
Chris Tselepis
Keyword(s):  
Colorectal Adenocarcinoma ◽  
In Vitro Proliferation ◽  
Cellular Iron ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Effects of platelet-rich plasma on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue

2021 ◽  
Vol 8 (1) ◽  
pp. 24
Author(s):  
Tie-ning Zhang ◽  
Quan Li ◽  
Te Ba ◽  
Tian-xi Shao ◽  
Fang Li ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Platelet Rich Plasma ◽  
Human Fibroblasts ◽  
In Vitro Proliferation ◽  
Scratch Assay ◽  
Fetal Bovine ◽  
And Migration ◽  
Wound Scratch Assay ◽  
Proliferation And Migration

Objective: To observe the effects of platelet-rich plasma (PRP) on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue.Methods: Fibroblasts were separated from human chronic refractory wound granulation tissue and then were identified. The obtained fibroblasts were divided into fetal bovine serum (FBS) group, hydrogel group and PRP group, and the three groups were cultured with culture mediums containing FBS, hydrogel and PRP respectively, in order to observe the growth of fibroblasts. The wound scratch assay was used to observe the migration of fibroblasts.Results: PRP group had more fibroblasts than FBS group and hydrogel group since Day 5 of culture, and exhibited greater fibroblast scratch migration area than FBS group on 48 h and 72 h of wound scratch assay (all p < .05).Conclusions: Compared with FBS, human fibroblasts cultured by PRP can more effectively promote the proliferation and migration of fibroblasts.

scholarly journals Visfatin Promotes Wound Healing Through the Activation of ERK1/2 and JNK1/2 Pathway

2018 ◽  
Author(s):  
Byungcheol Lee ◽  
Jisun Song ◽  
Arim Lee ◽  
Daeho Cho ◽  
Tae Sung Kim
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Human Keratinocytes ◽  
Dermal Fibroblasts ◽  
Dependent Manner ◽  
Vegf Expression ◽  
And Migration ◽  
Proliferation And Migration

Visfatin, a member of the adipokine family, plays an important role in many metabolic and stress responses. The mechanisms underlying the direct therapeutic effects of visfatin on wound healing have not been reported yet. In this study, we examined the effects of visfatin on wound healing in vitro and in vivo. Visfatin enhanced the proliferation and migration of human dermal fibroblasts (HDFs) and keratinocytes, and significantly increased the expression of wound healing-related vascular endothelial growth factor (VEGF) in vitro and in vivo. Treatment of HDFs with visfatin induced activation of both extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun N-terminal kinases 1 and 2 (JNK1/2) in a time-dependent manner. Inhibition of ERK1/2 and JNK1/2 led to a significant decrease in visfatin-induced proliferation and migration of HDFs. Importantly, blocking VEGF with its neutralizing antibodies suppressed the visfatin-induced proliferation and migration of HDFs and human keratinocytes, indicating that visfatin induces the proliferation and migration of HDFs and human keratinocytes via increased VEGF expression. Moreover, visfatin effectively improved wound repair in vivo, which was comparable to the wound healing activity of epidermal growth factor (EGF). Taken together, we demonstrate that visfatin promotes the proliferation and migration of HDFs and human keratinocytes by inducing VEGF expression and can be used as a potential novel therapeutic agent for wound healing.

scholarly journals Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Hyeon Ju Park ◽  
Guang-Ri Jin ◽  
Jae Hyun Jung ◽  
Su Bin Hwang ◽  
Su Hyun Lee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hair Loss ◽  
Hair Growth ◽  
Growth Patterns ◽  
Control Group ◽  
And Migration ◽  
Tgf Β1 ◽  
Proliferation And Migration ◽  
Nelumbinis Semen

This study investigated the hair regeneration promotion and hair loss prevention properties of Nelumbinis Semen (NS) extract in vitro and in vivo. The effect of NS on the proliferation and migration of human dermal papilla cells (hDPCs) was measured in vitro via CCK-8 and scratch migration assays, after which the antioxidant activity of NS was also quantified. NS extracts were then applied to the back of 7-week-old C57BL/6 mice for 3 weeks to monitor hair growth patterns and hair follicle (HF) histology. The mice were divided into three groups: negative control group (NC; DMSO), positive control group (PC; 3% minoxidil), and experimental group (NS extract 1,000 ppm). Moreover, to study the molecular mechanisms by which NS extract regenerates hair growth, real-time PCR was used to analyze factors related to the hair growth cycle. The NS extracts were found to possess high antioxidant properties due to their high flavonoid contents and electron-donating ability. Moreover, NS extracts enhanced hDPC proliferation and migration in a concentration-dependent manner (15.63–125 ppm). The hair growth index and growth area of the NS group (2.81 score, 81%) on day 14 were higher than those of the PC group (2.65 score, 68%) ( p < 0.05 ). Additionally, the HFs of the NS group were located deep in the subcutis, similar to the PC group with developed hair roots. Moreover, the mRNA expression of VEGF and IGF-1 was higher in the NS group compared to the PC group, whereas TGF-β1 expression was lower ( p < 0.05 ). Our findings indicate that NS modulates hair growth by increasing IGF-1 and VEGF expression while inhibiting that of TGF-β1. Therefore, our findings suggest that NS extract is a promising new hair loss treatment derived from a natural substance that helps promote hair growth and prevent hair loss.

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