Estrogenic effects of the ethyl-acetate extract of the stem bark of Erythrina lysistemon Hutch (Fabaceae)

Phytomedicine ◽  
2007 ◽  
Vol 14 (2-3) ◽  
pp. 222-226 ◽  
Author(s):  
F.S.F. Tanee ◽  
D. Njamen ◽  
C.B. Magne Ndé ◽  
J. Wanji ◽  
O. Zierau ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Estrogenic Effects

scholarly journals CYTOTOXIC BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA STEM BARK

2016 ◽  
Vol 8 (9) ◽  
pp. 111
Author(s):  
Kartini Hasballah ◽  
Murniana . ◽  
Erya . ◽  
Ardian .
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethyl Acetate Extract ◽  
Leukemia Cell ◽  
Stem Bark ◽  
Hexane Extract ◽  
Leukemia Cell Line ◽  
Calotropis Gigantea ◽  
Murine Leukemia Cell ◽  
Main Components

<p><strong>Objective: </strong>The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of <em>Calotropis gigantea</em> L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388.</p><p><strong>Methods: </strong>The crude extracts of <em>C. gigantea</em> stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS.</p><p><strong>Results: </strong>The qualitative screening of n-hexane extract of <em>Calotropis gigantea</em> L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC<sub>50 </sub>values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC<sub>50</sub> values 57.05 µg/ml and 52.58 µg/ml.</p><p><strong>Conclusion: </strong>Cytotoxic activity from E fraction of n-hexane extract of <em>C. gigantea</em> stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of <em>C. gigantea</em> are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol. </p>

scholarly journals PHENOL-2-(1-METHYL ETHOXY)-METHYL CARBAMATE COUMPOUND IN ETHYL ACETATE EXTRACT OF STEM BARK OF Aglaia angustifolia

2010 ◽  
Vol 8 (3) ◽  
pp. 463-468
Author(s):  
Sofnie M. Chairul
Keyword(s):  
Biological Activity ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Botanical Insecticide ◽  
Activity Test ◽  
Phase Mixture ◽  
Acetate Medium

Isolation of carbamate coumpound from ethyl acetate extract of stem bark of Aglaia angustifolia (Meliaceae), was carried out. The dried stem bark of A. angustifolia was extracted with ethanol (polar solvent), ethyl acetate (medium of polar) and water. From there extract solvent was biological activity test to Crocidolomia binotallis. Ethyl acetate extract solvent more active than another solvent, so that this extract was fractioned and clean up using chromatograpgy column, use SiO2 as stationary phase, mixture of n-hexane/ethyl acetate (10:1 ~ 1:1), ethyl acetate, and ethanol respectively as elution solution. The result of Biological activity test to C. binotallis showed that fraction of ethyl acetate inhibited growth on LC50 3.57 ppm. The compound of isolation result using HPLC, GCMS, FTIR and NMR was identified as phenol-2(1-methyl ethoxy) methyl carbamate coumpound, active as botanical insecticide.   Keywords: Meliaceae, A. angustifolia, carbamate, phenol-2 (1-methyl ethoxy) methyl carbamate

scholarly journals Four Flavan-3-ol Compounds From The Stembark of Chisocheton balansae C.DC. (MELIACEAE)

Molekul ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 9
Author(s):  
Unang Supratman ◽  
Mohamad Fajar ◽  
Supriatno Salam ◽  
Rani Maharani ◽  
Desi Harneti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
13C Nmr ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Methods ◽  
Endemic Plants ◽  
North Sulawesi ◽  
First Time

Chisocheton balansae C.DC., is one of the Meliaceae family plants which is the endemic plants from Soputan Mountain, North Sulawesi, Indonesia. This study was aimed to determine the chemical structure of flavan-3-ol compounds from ethyl acetate extract of C. balansae C.DC stembark. Dried powder of C. balansae C.DC stem bark was extracted consecutively with n-hexane, ethyl acetate, and methanol solvents. Four flavan-3-ol compounds, named catechin (1), epicatechin (2), epigallocatechin-3-O-gallate (3), and epicatechin-3-O-gallate (4) were successfully isolated from ethyl acetate extract. The chemical structure of these isolates was determined by spectroscopic methods (1H-NMR, 13C-NMR, DEPT, and 2D-NMR) and comparison with previous reported spectral data. These compounds are first time reported from this plant.

scholarly journals Antibacterial and antifungal activity of Moringa oleifera stem bark

2013 ◽  
pp. 109-117 ◽  
Author(s):  
Md Shafiqur Rahman ◽  
Laila Zerin ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Stem Bark ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

Petroleum ether, chloroform, ethyl acetate and carbon tetrachloride extracts of Moringa oleifera stem bark were studied for their antimicrobial activities against eleven human pathogenic bacteria (Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae) and four human pathogenic fungi (Aspergillus niger, A. ochraceus, A. ustus and Candida albicans) using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extracts exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extract exhibited the largest zone of inhibition (21 mm in diameter with 2000 mg/disc extract) against S. sonnei. The highest inhibition of fungal radial mycelial growth (52. 00% with 100 mg extract/ml medium) was recorded against C. albicans with ethyl acetate extract. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against B. megaterium, S. dysenteriae, V. cholerae and E. coli. For fungi, the lowest MIC was 500 mg/ml against C. albicans with crude extract of ethyl acetate. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13411 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):109-117, 2008

scholarly journals Triterpenoids from the Stem Bark of Crataeva nurvala

1970 ◽  
Vol 7 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Md Enamul Haque ◽  
Md Nahidul Islam ◽  
Dipankar Das Gupta ◽  
Mahbub Hossain ◽  
Hossain Uddin Shekhar ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Silica Gel ◽  
1H Nmr ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
First Report ◽  
Spectroscopic Analyses ◽  
Phytochemical Investigation

Two triterpenoids, phragmalin triacetate (1) and lupeol (2) were isolated from an ethyl acetate extract of the stem bark of Crataeva nurvala (Capparidaceae) by repeated chromatography over silica gel. The structures of these compounds were determined by spectroscopic analyses (UV, IR, 1H NMR, 13C NMR and EIMS). This is the first report of the systematic phytochemical investigation and the presence of these compounds 1 and 2 from this plant. Key words: Crataeva, Capparidaceae, Phragmalin triacetate and Lupeol.  DOI = 10.3329/dujps.v7i1.1221 Dhaka Univ. J. Pharm. Sci. 7(1): 71-74, 2008 (June)

scholarly journals Antimalarial Activity ofCroton macrostachyusStem Bark Extracts againstPlasmodium berghei In Vivo

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Jackie K. Obey ◽  
Moses M. Ngeiywa ◽  
Paul Kiprono ◽  
Sabah Omar ◽  
Atte von Wright ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Stem Bark ◽  
Significant Suppression ◽  
Innovative Drug ◽  
Croton Macrostachyus ◽  
Different Doses

There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to testin vivoantiplasmodial activity ofCroton macrostachyusH. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized withPlasmodium berghei(ANKA). Different doses (500, 250, and 100 mg/kg) ofC. macrostachyusethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters’ 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (p<0.001): for the ethyl acetate extract in the range of 58–82%, for the methanol extract in the range of 27–68%, for the aqueous extract in the range of 24–72%, and for the isobutanol extract in the range of 61–80%. Chemoprotective effect was significant (p<0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86–92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts ofC. macrostachyusin Balb/c mice supporting the folkloric use of the plant against malaria.

scholarly journals Screening for Bioactive Extracts and Targeted Isolation of Antimicrobial Agents from the Stem Bark of Adenanthera pavonina L.

2020 ◽  
pp. 227-242
Author(s):  
K. Abdu ◽  
M. Adamu
Keyword(s):  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Extract ◽  
Scientific Evidence ◽  
Bacterial Species ◽  
Stem Bark ◽  
Artemia Salina ◽  
Scientific Data ◽  
Adenanthera Pavonina ◽  
Solvent Extract

Bioactive secondary metabolites can be isolated from medicinal plants as antimicrobial agents. Based on ethnopharmacological relevance, Adenanthera pavonina L. is recognized as a plant with good medicinal values and forms the integral part systems of traditional medicine in Borno state, and other parts of Nigeria. Despite the widespread used, most of the information available to consumers about the plant is not backed by credible scientific data. Only a limited number of studies have probed into the scientific evidence for bioactivity and safety of this medicinal plant. Such studies rarely progress to isolation and evaluation of the active antimicrobial agents. In the present study, four solvent-extract (ethanol, ethyl acetate, chloroform and n-hexane) were subjected to antimicrobial activity test against E. coli (E.C), S. typhi (S.T), S. aureus (S.A), A. flavus (A.F), C. albican (C.A) and M. specie (M.S) and toxicity test against Artemia salina. The ethyl acetate extract showed outstanding activity against the microbes (bacteria and fungi) and Artemia salina, respectively. The activity and toxicity effects of the ethyl acetate extract suggests the presence of active antimicrobial agents and hence provides a way forward for column chromatographic isolation of the targeted Antimicrobial agents. The targeted compounds isolated (AP-X38 and AP-X44) were also subjected to antimicrobial test using three bacterial species and found to be even more active that the solvent extract of ethyl acetate. This study justifies part of ethno medicinal claims on the plant.

scholarly journals ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

2019 ◽  
Vol 19 (3) ◽  
pp. 58-63
Author(s):  
Ilham Maulana ◽  
Binawati Ginting ◽  
Nurdin Nurdin ◽  
Saiful Fakri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Myristica Fragrans ◽  
Ic50 Value ◽  
Stem Bark Extract ◽  
Myristica Fragrans Houtt

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared  to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

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