scholarly journals Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.


Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Eis Nurhiliya

ABSTRAK              Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak tanaman sereh (Andropogon nardus), biji mahoni (Switenia macrophylla) dan kombinasi keduanya terhadap mortalitas larva nyamuk Aedes aegypti. Larva nyamuk Aedes aegypti untuk sampel adalah larva hasil rearing F2 yang diperoleh dengan ovitrap dari wilayah Kelurahan Kambu yang merupakan wilayah endemis Demam Berdarah Dengue (DBD). Penelitian ini dilakukan di laboratorium Zoologi, FMIPA UHO, Kendari. Ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) masing-masing dengan konsentrasi 1,5% dan 3,0% dan 4.5%. kombinasi kedua jenis ekstrak tersebut dipaparkan selama 24 jam untuk melihat mortalitas larva nyamuk Aedes aegypti. Pengumpulan data dilakukan dengan menghitung mortalitas larva Aedes aegypti dalam setiap jam pengamatan. Hasil penelitian menunjukkan bahwa, perlakuan dengan ekstrak biji mahoni (Switenia macrophylla) konsentrasi 4,5% menyebabkan mortalitas larva mencapai 100%, sedangkan ekstrak sereh (Andropogon nardus) konsentrasi 4,5% menyebabkan mortalitas larva tertinggi (98%) dalam 24 jam pemaparan. Pada kombinasi ekstrak tanaman sereh (Andropogon nardus) dan biji mahoni (Switenia macrophylla) dengan konsentrasi masing-masing 3.0% +3.0% dan  4,5%+4.5% menyebabkan mortalitas larva mencapai 100%. Berdasarkan WHO (2009), perlakuan ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) dengan konsentrasi tersebut efektif sebagai larvasida untuk larva Aedes aegypti. Kata kunci: Mortalitas, larva Aedes aegypti, ekstrak sereh (Andropogon nardus), ekstrak biji mahoni (Switenia macrophylla). ABSTRACT This study aims to determine the effect of sereh grass extract (Andropogon nardus), mahogany seeds (Switenia macrophylla) and a combination of both of them to the mortality of Aedes aegypti mosquito larvae. Aedes aegypti mosquito larvae for the sample were rearing F2 larvae obtained by ovitrap from the Kambu Village area which was an endemic area of Dengue Hemorrhagic Fever (DHF). This research was conducted at the Zoology laboratory, FMIPA UHO Kendari. Lemon grass extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) each with a concentration of 1.0% and 3.0% and a combination of the two types of extract were exposed for 24 hours to see the mortality of Aedes aegypti mosquito larvae. Data collection was done by calculating the mortality of Aedes aegypti larvae in each observation hour. The results showed that treatment with mahogany seed extract (Switenia macrophylla) concentration of 4.5% caused larval mortality to reach 100%, while lemongrass extract (Andropogon nardus) concentration of 4.5% caused the highest larval mortality (98%) in 24 hours of exposure. The combination of lemongrass extract (Andropogon nardus) and mahogany seeds (Switenia macrophylla) with a concentration of 3.0%+3.0% and 4.5% + 4.5% caused larval mortality to reach 100%. Based on WHO (2009), the treatment of citronella extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) at that concentration were effective  as larvacidal for larval Aedes aegypti mosqouitoes. Keywords: Mortality, Aedes aegypti, Plant Extracts of Lemon Grass (Andropogon nardus), Mahogany Seed Extract (Switenia macrophylla)


Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
Firza Khairullah Sembiring ◽  
Riyani Susan BT Hasan

This research was conducted to determine the effectiveness of betel leaf extract conducted at a concentration of 2.5%, 5.0%, 7.5%, 10% as larvicide toward the development of Aedes Aegypti Sp. Aedes Aegypti Sp larvae are dangerous vectors in Dengue Hemorrhagic Fever (DHF) in humans. The method in this research was carried out on an experimental method by using a post-test only design and sampling with a purposive sampling method. The effectiveness of betel leaf extract larvicide on the development of Aedes Aegypti Sp larvae was carried out by mixing betel leaf extract with several concentrations in one container Aedes Aegypti Sp larvae inside. The results obtained in this research were carried out by the Kruskal-Wallis test, the test found that there was a significant difference because it was given a 95% confidence index. The larvae treated with betel leaf extract with a concentration of 10% had the highest effectiveness in stopping the development of Aedes Aegypti Sp larvae, which proved at the mortality at a concentration of 10%, found total mortality of 20 larvae in 8 hours of treatment. Betel leaf extract with a concentration of 10% had the highest effectiveness of discontinuing the development of Aedes Aegypti Sp larvae.


2018 ◽  
Vol 2 (1) ◽  
pp. 36
Author(s):  
Setyo Dwi Santoso ◽  
Abdul Chamid ◽  
Dwi Viddi Kusuma Pratiwi

Dengue hemorrhagic fever (DHF) is a very dangerous disease, caused by Aedes aegypti mosquitoes. One alternative to controlling Aedes aegypti mosquitoes is to use insecticides. But it would be much better if the insecticides used are natural and environmentally friendly, one of them by using Tomato Leaf Extract (Solanum lycopersicum l.). Tomato leaves contain chemical compounds class Alkaloids and Saponins and can serve as anti larvacide. This research is a laboratory experiment which aims to find out the effect of tomato leaf extract (Solanum lycopersicum L.) activity on the death of III instar larvae of Aedes aegypti mosquito. the effectiveness of samples on Aedes aegypti mosquito larvae by treatment of variation of extract concentration 0.2%, 0.4%, 0.6%, 0.8%, 1% with repetition 2 times. In the results of research showed that the smallest concentration that can kill Aedes aegypti mosquito larvae is 0.6% at 16 hours and probit analysis showed that significance is 0,608 greater than intercept 0.003, it shows that the extract of tomato leaves affects the death of III instar larvae of Aedes aegypti mosquito.


2017 ◽  
Vol 5 (2) ◽  
pp. 113-120
Author(s):  
Marjan Wahyuni ◽  
Rusdi Rusdi

Dengue Haemorrhagic Fever (DHF) is a disease that is still a major problem in Indonesia. Until now there is no cure or vaccine to combat dengue, so the only way is to break the chain of transmission of mosquito Aedes aegypti is the prevention or control of disease vectors. This study aims to determine the Efficiency of Mixed Leaf Extract (Pipper Betle) and Leaf Yam Extract with Temephos On Mortality of Aedes aegypti. This research is experimental and research will be conducted in the laboratory of environmental health. The number of treatment in this study was 3 treatment with different doses of 0.5 mg / l ; 1.0 mg / l ; 1.5 g / l ; 2m g / l and 1 control (0 mg / liter) were treated in the larvae mosquitoes. The results showed that the air temperature at the study site averaged 27oC, the air humidity between 78-79%, the water temperature that became the Aedes aegypti breeding 26-27oC, the average pH 6.8-7. The results showed that the leaf extract beta and bengkuang leaves at a dose of 2.0 mg / l for 24 hours were able to kill Aedes aegypti larvae of 96.6%, while abates with a dose of 2.0 mg / l for 24 hours were able to kill Aedes aegypti larva by 70%. After statistical analysis with Independent t-test showed that betel leaf extract and bengkuang leaf gave a significant effect on the mortality of Aedes aegypti larvae. So it can be concluded that there is a significant effectiveness difference between bengkuang leaf extract (Pachyrrhyzus erosus) and betel leaf (Piper betle) with abate to mortality of Aedes aegypti larvae. for the mortality of Aedes aegypti larvae both in terms of average mortality (p = 0.003) and in terms of mean duration (p = 0,000) and there was a significant relationship (p = 0,000) between giving both larvacides to Aedes aegypti larvae mortality. This research suggests to the public to use organic larvacides and to reduce the use of abate.


Author(s):  
Wihdatul Karima ◽  
Syahrul Ardiansyah

Indonesia has a potential to be exposed to the threat of dengue hemorrhagic fever with the point vector, the Aedes aegypti mosquito, so development need to be stopped. The use of synthetic larvicides in eradicating mosquito larvae can cause environmental pollution. To reduce it, an alternative is needed using plant larvacides with banana leaf. Banana leaves are obtained from Candi Sidoarjo. Ethanol extract of banana leaf contains tannin compounds, alkaloids, terpenoids, saponins, and flavonoids which can be used as larvicides. This research was conducted to determine the toxic effects of banana leaf extract (Musa paradisiaca L.) on the larvae of Aedes aegypti mosquito mortality. This research was conducted using the post test only the control group design with 6 treatment groups including control (aquades) and banana leaf  extract concentrations of 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, 5000 ppm. This study used third instar larvae, each test group countaining 20 larvae with 4 repetitions. That obtained were analyzed using data and probit tests. The results of this study that banana leaf extract has a toxic effect on Aedes aegypti mosquito larvae with LC50 at a concentration of 4638 ppm.


2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies


2021 ◽  
Vol 9 (1) ◽  
pp. 26-30
Author(s):  
Firdha Firdha Novitasari ◽  
Dwi Wahyuni Dwi Wahyuni ◽  
Erma Sulistyaningsih Erma Sulistyaningsih

Introduction: Dengue hemorrhagic fever (DHF) is an important public health problem in Indonesia because its morbidity and mortality are quite high. Prevention by vector control methods with chemical insecticides adversely affects the environment. Research on granular bioinsectide mixture of betel leaf extract and sugar apple seeds against Aedes aegypti is expected to be used to break the chain of Aedes aegypti mosquitoes starting  from the juvenile stage  (eggs) as an effort to control the vector population naturally. Objective: This study aims to analyze the effect of granule bioinsecticide mixture of sugar apple seed extract and betel leaf on changes in the morphology of Aedes aegypti eggs. Methods: This study is a true-experimental study with a Post test Only Control Group Design design. The treatment group will be given a granular bioinsecticide, a mixture of sugar apple seed extract and betel leaf, positive control will be given abate while negative control will be given aquades. Each group uses 25 eggs with 3 repetitions. On day 3, all eggs will be observed using a stereo microscope to determine morphological changes. The data obtained will be analyzed using ordinal regression test. Results: the ordinal regression test got the value of ρ in the Wald test between (0.012 - 0.955)> α = 0.01. Conclusion: there is no effect of mixed granules of betel leaf and sugar apple seeds on the mortality of Aedes aegypti eggs. Further research is needed on new formulations using other natural ingredients to increase the effect of ovicides on Aedes aegypti.


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