Hydrogen sulfide alleviates chilling injury of banana fruit by enhanced antioxidant system and proline content

AbstractThe antioxidant system effects of Kandelia candel were investigated under four different levels of PAH stress. The activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), the responses to the change of malondialdehyde (MDA) contents and the accumulation of proline in K. candel were determined. Our results suggested that the activities of SOD, CAT, POD increased significantly in leaves and roots of K. candel (p≤0.05) with the increase of the external PAH concentrations, while in stems, the activities of these antioxidant enzymes were all significantly inhibited (p≤0.01). We also observed an increase of MDA in leaves, stems and roots, and an obvious correlation between MDA content and PAH concentrations in three locations, which showed that the change of MDA content could be used as a biomarker of K. candel under PAH stress. The proline content was found remarkably enhanced in leaves, stems and roots. However, a significant inverse correlation was observed between the proline content and SOD (r=−0.99, p≤0.01), POD (r=−0.95, p≤0.05) activities in stems. This study suggested that the antioxidative system of K. candel has an obvious organ-dependent feature when exposed to PAH contamination as revealed by discriminant analysis (DA).

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Transcriptome analysis of postharvest blueberries (Vaccinium corymbosum‘Duke’) in response to cold stress

10.21203/rs.2.16686/v3 ◽

2020 ◽

Author(s):

Fan Zhang ◽

ShuJuan Ji ◽

BaoDong Wei ◽

Shunchang Cheng ◽

Jia Hao ◽

...

Keyword(s):

Cold Stress ◽

Cold Storage ◽

Stress Responses ◽

Chilling Injury ◽

Membrane Lipid ◽

Proline Content ◽

Cdna Libraries ◽

Transcriptional Level ◽

Biological Processes ◽

As Stress

Abstract Background: Blueberry ( Vaccinium spp. ) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displayed as pedicle pits; and the samples of 0°C-30 days was the critical point of CI. However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely. Methods: To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in 0d (control) and 30d chilled blueberry. At the same time, the pitting and decay rate, electrolyte leakage (EL), malondialdehyde (MDA) proline content and GSH content were also measured. Results: Two cDNA libraries from 0d (control) and 30d chilled samples were constructed and sequenced, generating a total of 35,060 unigenes with an N50 length of 1,348bp. Of these, 1852 were differentially expressed, with 1,167 upregulated and 685 downregulated. Forty-five cold-induced transcription factor (TF) families containing 1,023 TFs were identified. The DEGs indicated in biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to cold storage. The transcriptional level of 40 DEGs were verified by qRT-PCR. Conclusions: The postharvest cold storage leads serious CI in blueberry, which substantially decreases the quality, storability and consumer acceptance. The MDA content, proline content, EL increased and the GSH content decreased in this chilled process. The biological processes such as stress responses, hormone metabolic processes were significantly affected by CI. Overall, the results obtained here are valuable for preventing CI under cold storage and could help to perfect the lack of the genetic information of non-model plant species.

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Participation of Hydrogen Sulfide in Induction of Antioxidant System in Roots of Wheat Plantlets and Their Heat Resistance by Salicylic Acid

Applied Biochemistry and Microbiology ◽

10.1134/s0003683820040079 ◽

2020 ◽

Vol 56 (4) ◽

pp. 467-472 ◽

Cited By ~ 1

Author(s):

Yu. V. Karpets ◽

M. A. Shkliarevskyi ◽

E. I. Horielova ◽

Yu. E. Kolupaev

Keyword(s):

Salicylic Acid ◽

Hydrogen Sulfide ◽

Heat Resistance ◽

Antioxidant System

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Effects of salicylic acid and ultrasound treatments on chilling injury control and quality preservation in banana fruit during cold storage

Scientia Horticulturae ◽

10.1016/j.scienta.2019.02.018 ◽

2019 ◽

Vol 249 ◽

pp. 334-339 ◽

Cited By ~ 13

Author(s):

Orang Khademi ◽

Meysam Ashtari ◽

Farhang Razavi

Keyword(s):

Salicylic Acid ◽

Cold Storage ◽

Chilling Injury ◽

Banana Fruit ◽

Injury Control

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Revealing Further Insights on Chilling Injury of Postharvest Bananas by Untargeted Lipidomics

Foods ◽

10.3390/foods9070894 ◽

2020 ◽

Vol 9 (7) ◽

pp. 894 ◽

Cited By ~ 1

Author(s):

Juan Liu ◽

Qingxin Li ◽

Junjia Chen ◽

Yueming Jiang

Keyword(s):

Fatty Acids ◽

Low Temperature ◽

Unsaturated Fatty Acids ◽

Membrane Lipids ◽

Saturated Fatty Acids ◽

Chilling Injury ◽

Membrane Damage ◽

Membrane Lipid ◽

Banana Fruit ◽

Control Fruit

Chilling injury is especially prominent in postharvest bananas stored at low temperature below 13 °C. To elucidate better the relationship between cell membrane lipids and chilling injury, an untargeted lipidomics approach using ultra-performance liquid chromatography–mass spectrometry was conducted. Banana fruit were stored at 6 °C for 0 (control) and 4 days and then sampled for lipid analysis. After 4 days of storage, banana peel exhibited a marked chilling injury symptom. Furthermore, 45 lipid compounds, including glycerophospholipids, saccharolipids, and glycerolipids, were identified with significant changes in peel tissues of bananas stored for 4 days compared with the control fruit. In addition, higher ratio of digalactosyldiacylglycerol (DGDG) to monogalactosyldiacylglycerol (MGDG) and higher levels of phosphatidic acid (PA) and saturated fatty acids but lower levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and unsaturated fatty acids were observed in banana fruit with chilling injury in contrast to the control fruit. Meanwhile, higher activities of phospholipase D (PLD) and lipoxygenase (LOX) were associated with significantly upregulated gene expressions of MaPLD1 and MaLOX2 and higher malondialdehyde (MDA) content in chilling injury-related bananas. In conclusion, our study indicated that membrane lipid degradation resulted from reduced PC and PE, but accumulated PA, while membrane lipid peroxidation resulted from the elevated saturation of fatty acids, resulting in membrane damage which subsequently accelerated the chilling injury occurrence of banana fruit during storage at low temperature.

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