Exogenous ethylene alleviates chilling injury of ‘Huangguan’ pear by enhancing the proline content and antioxidant activity

2019 ◽  
Vol 257 ◽  
pp. 108671 ◽  
Author(s):  
Chuangqi Wei ◽  
Lihua Ma ◽  
Yudou Cheng ◽  
Yeqing Guan ◽  
Junfeng Guan
Keyword(s):  
Antioxidant Activity ◽  
Chilling Injury ◽  
Proline Content ◽  
Exogenous Ethylene

scholarly journals Transcriptome analysis of postharvest blueberries (Vaccinium corymbosum‘Duke’) in response to cold stress

2020 ◽  
Author(s):  
Fan Zhang ◽  
ShuJuan Ji ◽  
BaoDong Wei ◽  
Shunchang Cheng ◽  
Jia Hao ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Storage ◽  
Stress Responses ◽  
Chilling Injury ◽  
Membrane Lipid ◽  
Proline Content ◽  
Cdna Libraries ◽  
Transcriptional Level ◽  
Biological Processes ◽  
As Stress

Abstract Background: Blueberry ( Vaccinium spp. ) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displayed as pedicle pits; and the samples of 0°C-30 days was the critical point of CI. However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely. Methods: To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in 0d (control) and 30d chilled blueberry. At the same time, the pitting and decay rate, electrolyte leakage (EL), malondialdehyde (MDA) proline content and GSH content were also measured. Results: Two cDNA libraries from 0d (control) and 30d chilled samples were constructed and sequenced, generating a total of 35,060 unigenes with an N50 length of 1,348bp. Of these, 1852 were differentially expressed, with 1,167 upregulated and 685 downregulated. Forty-five cold-induced transcription factor (TF) families containing 1,023 TFs were identified. The DEGs indicated in biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to cold storage. The transcriptional level of 40 DEGs were verified by qRT-PCR. Conclusions: The postharvest cold storage leads serious CI in blueberry, which substantially decreases the quality, storability and consumer acceptance. The MDA content, proline content, EL increased and the GSH content decreased in this chilled process. The biological processes such as stress responses, hormone metabolic processes were significantly affected by CI. Overall, the results obtained here are valuable for preventing CI under cold storage and could help to perfect the lack of the genetic information of non-model plant species.

Antioxidant activity and proline content of leaf extracts from Dorystoechas hastata

2008 ◽  
Vol 111 (2) ◽  
pp. 400-407 ◽  
Author(s):  
Arife Alev Karagözler ◽  
Bengi Erdağ ◽  
Yelda Çalmaz Emek ◽  
Deniz Aktaş Uygun
Keyword(s):  
Antioxidant Activity ◽  
Proline Content ◽  
Leaf Extracts

scholarly journals Effect of the Application of 1-Methylcyclopropene and Wax Emulsions on Proximate Analysis and Some Antioxidants of Soursop (Annona muricataL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Cristina L. Moreno-Hernández ◽  
Sonia G. Sáyago-Ayerdi ◽  
Hugo S. García-Galindo ◽  
Miguel Mata-Montes De Oca ◽  
Efigenia Montalvo-González
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Total Phenolics ◽  
Chilling Injury ◽  
Proximate Analysis ◽  
Nutritional Composition ◽  
Composition Analysis ◽  
Postharvest Handling ◽  
Phenolics Content

The effect of the application of 1-methylcyclopropene (1-MCP) and wax emulsions, alone or combined, on composition analysis, vitamin C, polyphenols, and antioxidant capacity of soursop was evaluated. Fruits were stored as follows: at 25°C (control), and at 16°C: fruits sprayed with candelilla or flava emulsions, fruits treated with 1500 nL/L of 1-MCP (20°C, 12 h), and fruits treated with 1-MCP and then sprayed with emulsions. Fruits were allowed to ripen and the edible part was used for analysis. Only fruits stored at 16°C without 1-MCP showed visible symptoms of chilling injury. Fruits treated with 1-MCP combined with flava emulsion maintained in greater extent their vitamin C content, dietary fiber, total phenolics content, and antioxidant activity. The combination of 1-MCP and emulsions can be utilized in postharvest handling of soursop because this combination can preserve its nutritional composition and antioxidant activity.

scholarly journals Optimization of Postharvest Storage Conditions to Maintain Fruit Quality and Health Maintaining Properties of Grapefruit

2010 ◽  
Author(s):  
Bhimanagouda S. Patil ◽  
Ron Porat ◽  
G.K. Jayaprakasha ◽  
K.N.C. Murthy
Keyword(s):  
Antioxidant Activity ◽  
Cold Storage ◽  
Chilling Injury ◽  
Modified Atmosphere Packaging ◽  
Storage Period ◽  
Storage Conditions ◽  
Hydrogen Atom Transfer ◽  
Conditioning Experiment ◽  
Conditioning Treatment ◽  
Different Temperatures

Antioxidant activity of fruits is gaining wide interest among consumers due to its importance in counteracting oxidative stress, free radicals and preventing DNA damage. Oxygen radical absorbance capacity (ORAC) assay is one of the commonly used assays to measure the antioxidant activity, which is based on hydrogen atom transfer mechanism. Furocoumarins present in grapefruit are reported to have antiproliferative activity, induce GST activity, inhibit biofilm formation and increase bioavailability of drugs. In the present project ORAC values were measured of Star Ruby grapefruit undergone ethylene degreening treatment, cold storage and temperature conditioning treatment, and modified atmosphere packaging which were stored at different temperatures for prolonged period. In addition, furocoumarins were quantified in Star Ruby grapefruits from cold storage and conditioning experiment conducted in Israel. Conditioning treatment is practiced prior cold storage to reduce chilling injury in grapefruits during cold storage for prolonged period. Levels of 6,7-dihyrdoxy bergamottin decreased during storage period in all three treatments.

scholarly journals Effect of Ethylene on Cell Wall and Lipid Metabolism during Alleviation of Postharvest Chilling Injury in Peach

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1612 ◽  
Author(s):  
Yongchao Zhu ◽  
Ke Wang ◽  
Chunxia Wu ◽  
Yun Zhao ◽  
Xueren Yin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lipid Metabolism ◽  
Chilling Injury ◽  
Response Factors ◽  
Ethylene Treatment ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Endogenous Ethylene ◽  
Highly Correlated ◽  
Exogenous Ethylene

Peach is prone to postharvest chilling injury (CI). Here it was found that exogenous ethylene alleviated CI, accompanied by an increased endogenous ethylene production. Ethylene treatment resulted in a moderately more rapid flesh softening as a result of stronger expression of genes encoding expansin and cell wall hydrolases, especially xylosidase and galactosidase. Ethylene treatment alleviated internal browning, accompanied by changes in expression of polyphenol oxidase, peroxidase and lipoxygenases. An enhanced content of phospholipids and glycerolipids and a reduced content of ceramide were observed in ethylene-treated fruit, and these were associated with up-regulation of lipid phosphate phosphatase, fatty acid alpha-hydroxylase, and golgi-localized nucleotide sugar transporter, as well as down-regulation of aminoalcohol phosphotransferases. Expression of two ethylene response factors (ERFs), ESE3 and ABR1, was highly correlated with that of genes involved in cell wall metabolism and lipid metabolism, respectively. Furthermore, the expression of these two ERFs was strongly regulated by ethylene treatment and the temperature changes during transfer of fruit into or out of cold storage. It is proposed that ERFs fulfill roles as crucial integrators between cell wall modifications and lipid metabolism involved in CI processes ameliorated by exogenous ethylene.

scholarly journals Allelopathic Potential of Haloxylon persicum against Wheat and Black Mustard with Special Reference to Its Phytochemical Composition and Antioxidant Activity

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 244
Author(s):  
Ibrahim Bayoumi Abdel-Farid ◽  
Mohamed Salah Massoud ◽  
Yahia Al-Enazy ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Muhammad Jahangir ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Seed Germination ◽  
Dry Weight ◽  
Proline Content ◽  
Shoot Length ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Wheat Seedlings ◽  
Extract Concentration ◽  
Black Mustard

Haloxylon persicum is a well-known plant of sandy deserts, but yet, its allelopathic and phytochemical potential has not been explored. In the present study, we evaluated the effects of different concentrations (1, 2, 4, and 8%) of the aqueous extract of H. persicum shoots on seed germination and seedling growth of wheat crop and black mustard weed. The effects of the same extract on the biochemical parameters (chlorophyll a, chlorophyll b, carbohydrates content, and proline content) of wheat seedlings were also investigated. Low and moderate concentrations (1, 2, and 4%) of H. persicum aqueous extract nonsignificantly affected seed germination, shoot length, root length, fresh weight, and dry weight of wheat seedlings, whereas these parameters were negatively affected by the application of the highest extract concentration (8%). The results revealed a significant increase in proline content in wheat seedlings under all the applied concentrations of H. persicum extract. Low and moderate concentrations (1–4%) of H. persicum extract significantly enhanced chlorophyll a, chlorophyll b, and total chlorophyll content in wheat seedlings, whereas these parameters were significantly reduced when the highest extract concentration (8%) was applied. Only the lowest concentration (1%) of H. persicum extract reduced the carbohydrates content of wheat seedlings, while other concentrations showed nonsignificant effects. Concentrations of 4 and 8% of H. persicum extract reduced the seed germination percentage of black mustard. The root length, shoot length, and fresh weight of black mustard seedlings were significantly decreased by the application of 1 and 2% extract concentrations and completely inhibited in response to treatment with 4 and 8% concentrations. The dry weight of black mustard seedlings was not affected by 1 and 2% extract concentrations but completely inhibited at 4 and 8% concentrations. The methanol extract of H. persicum contains high contents of bioactive secondary metabolites, such as phenolics, flavonoids, flavonols, anthocyanins, tannins, saponins, as well as proved to has a very strong antioxidant activity of the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH). Based on our results, we recommend the potential application of the aqueous extracts of H. persicum to control black mustard weed in wheat crops. Furthermore, H. persicum revealed an interesting phytochemical pattern with sound antioxidant activity that assigns this plant as a promising candidate to explore its detailed metabolomics profile along with potential bioactivities.

scholarly journals Transcriptome analysis of postharvest blueberries (Vaccinium corymbosum ‘Duke’) in response to cold stress

2019 ◽  
Author(s):  
Fan Zhang ◽  
ShuJuan Ji ◽  
BaoDong Wei ◽  
Shunchang Cheng ◽  
Jia Hao ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Storage ◽  
Stress Responses ◽  
Chilling Injury ◽  
Vaccinium Corymbosum ◽  
Proline Content ◽  
Cdna Libraries ◽  
Transcriptional Level ◽  
Biological Processes ◽  
As Stress

Abstract Background: Blueberry (Vaccinium spp.) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displayed as pedicle pits; and the samples of 0°C-30 days was the critical point of CI. However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely. Methods: To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in 0d (control) and 30d chilled blueberry. At the same time, the pitting and decay rate, electrolyte leakage (EL), malondialdehyde (MDA) proline content and GSH content were also measured.Results: Two cDNA libraries from 0d (control) and 30d chilled samples were constructed and sequenced, generating a total of 35,060 unigenes with an N50 length of 1,348bp. Of these, 1852 were differentially expressed, with 1,167 upregulated and 685 downregulated. Forty-five cold-induced transcription factor (TF) families containing 1,023 TFs were identified. The DEGs indicated in biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to cold storage. The transcriptional level of 40 DEGs were verified by qRT-PCR. Conclusions: The postharvest cold storage leads serious CI in blueberry, which substantially decreases the quality, storability and consumer acceptance. The MDA content, proline content, EL increased and the GSH content decreased in this chilled process. The biological processes such as stress responses, hormone metabolic processes were significantly affected by CI. Overall, the results obtained here are valuable for preventing CI under cold storage and could help to perfect the lack of the genetic information of non-model plant species.

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