Expression patterns of genes involved in sugar metabolism and accumulation during peach fruit development and ripening

Two cultivars of F. vesca, red-fruited Baron Solemacher (BS) and white-fruited Pineapple Crush (PC), were studied to compare and contrast the quantitative accumulation of major polyphenols and related biosynthetic pathway gene expression patterns during fruit development and ripening. Developing PC fruit showed higher levels of hydroxycinnamic acids in green stages and a greater accumulation of ellagitannins in ripe fruit in comparison to BS. In addition to anthocyanin, red BS fruit had greater levels of flavan-3-ols when ripe than PC. Expression patterns of key structural genes and transcription factors of the phenylpropanoid/flavonoid biosynthetic pathway, an abscisic acid (ABA) biosynthetic gene, and a putative ABA receptor gene that may regulate the pathway, were also analyzed during fruit development and ripening to determine which genes exhibited differences in expression and when such differences were first evident. Expression of all pathway genes differed between the red BS and white PC at one or more times during development, most notably at ripening when phenylalanine ammonia lyase 1 (PAL1), chalcone synthase (CHS), flavanone-3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and UDP:flavonoid-O-glucosyltransferase 1 (UFGT1) were significantly upregulated in the red BS fruit. The transcription factors MYB1 and MYB10 did not differ substantially between red and white fruit except at ripening, when both the putative repressor MYB1 and promoter MYB10 were upregulated in red BS but not white PC fruit. The expression of ABA-related gene 9-cis-epoxycarotenoid dioxygenase 1 (NCED1) was higher in red BS fruit but only in the early green stages of development. Thus, a multigenic effect at several points in the phenylpropanoid/flavonoid biosynthetic pathway due to lack of MYB10 upregulation may have resulted in white PC fruit.

Download Full-text

Dynamic Changes of Ascorbic Acid Occurring During Fruit Development and Ripening of Actinida Latifolia and Their Associated Molecular Mechanisms Deciphered by Weighted Gene Co-Expression Network Analysis

10.21203/rs.3.rs-133017/v1 ◽

2020 ◽

Author(s):

Hui Xia ◽

Honghong Deng ◽

Rongping Hu ◽

Lijin Lin ◽

Jin Wang ◽

...

Keyword(s):

Ascorbic Acid ◽

Network Analysis ◽

Fruit Development ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Network Module ◽

Dynamic Changes ◽

Fruit Development And Ripening ◽

Expansion Stage ◽

Highly Correlated

Abstract Background: Actinidia latifolia is an exceptional source with extremely high ascorbic acid (AsA) content. However, its transcriptome atlas is lacking and how AsA accumulates during fruit development and ripening of this special kiwifruit and its associated molecular mechanisms are still poorly understood.Results: Herein, the dynamic changes of AsA content of six stages of A. latifolia fruit development and ripening determined by HPLC demonstrated a rapid increasing profile during the initial expansion stage with a peak around 60 days after flowering (1194.21±69.25 mg 100g-1 FW), followed by a progressive, albeit not significant decrease tendency and reached the minimum levels (1028.76±31.19 mg 100g-1 FW) at maturity. A high-quality full-length (FL) transcriptome of A. latifolia was successfully constructed by third-generation sequencing for the first time, comprising 326,926 FL non-chimeric reads, 15,505 coding sequences, 2882 transcription factors, 18,797 simple sequence repeats, 3328 long noncoding RNAs, and 231 alternative splicing events. Illumina RNA-seq in combination with weighted gene co-expression network analysis revealed a network module highly correlated (r=0.5, p=0.03) with AsA content. Gene co-expression in this network module was explained by its roles in protein processing in endoplasmic reticulum (ko04141), glycolysis/gluconeogenesis (ko00010), and carbon metabolism (ko01200). Moreover, the expression patterns of genes involved in AsA biosynthesis and metabolism validated by qRT-PCR exhibited a similar trend with AsA accumulation.Conclusions: Overall, the dynamic changes of AsA content and associated key genes and enriched metabolic pathways were deciphered, which paves the way for genetic improvement that aims for development of kiwifruit with super-high AsA content.

Download Full-text

Genome-Wide Analysis of the MADS-Box Transcription Factor Family in Solanum lycopersicum

International Journal of Molecular Sciences ◽

10.3390/ijms20122961 ◽

2019 ◽

Vol 20 (12) ◽

pp. 2961 ◽

Cited By ~ 4

Author(s):

Yunshu Wang ◽

Jianling Zhang ◽

Zongli Hu ◽

Xuhu Guo ◽

Shibing Tian ◽

...

Keyword(s):

Gene Family ◽

Fruit Development ◽

Expression Patterns ◽

Functional Characterization ◽

Constitutive Expression ◽

Floral Organ ◽

Mads Box ◽

Mads Box Genes ◽

Fruit Development And Ripening ◽

Mads Box Gene

MADS-box family genes encode transcription factors that are involved in multiple developmental processes in plants, especially in floral organ specification, fruit development, and ripening. However, a comprehensive analysis of tomato MADS-box family genes, which is an important model plant to study flower fruit development and ripening, remains obscure. To gain insight into the MADS-box genes in tomato, 131 tomato MADS-box genes were identified. These genes could be divided into five groups (Mα, Mβ, Mγ, Mδ, and MIKC) and were found to be located on all 12 chromosomes. We further analyzed the phylogenetic relationships among Arabidopsis and tomato, as well as the protein motif structure and exon–intron organization, to better understand the tomato MADS-box gene family. Additionally, owing to the role of MADS-box genes in floral organ identification and fruit development, the constitutive expression patterns of MADS-box genes at different stages in tomato development were identified. We analyzed 15 tomato MADS-box genes involved in floral organ identification and five tomato MADS-box genes related to fruit development by qRT-PCR. Collectively, our study provides a comprehensive and systematic analysis of the tomato MADS-box genes and would be valuable for the further functional characterization of some important members of the MADS-box gene family.

Download Full-text

MicroRNA Profiling During Mulberry (Morus atropurpurea Roxb) Fruit Development and Regulatory Pathway of miR477 for Anthocyanin Accumulation

Frontiers in Plant Science ◽

10.3389/fpls.2021.687364 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xiaonan Dong ◽

Chaorui Liu ◽

Yuqi Wang ◽

Qing Dong ◽

Yingping Gai ◽

...

Keyword(s):

Fruit Development ◽

Target Genes ◽

Expression Patterns ◽

Regulation Of Transcription ◽

Anthocyanin Accumulation ◽

Regulatory Pathway ◽

Development Stages ◽

Fruit Development And Ripening ◽

Non Coding Rnas ◽

Mulberry Fruits

To understand the mechanism of small non-coding RNAs (miRNA)-mediated development and ripening of mulberry fruits, three small RNA libraries from mulberry fruits at different development stages were constructed, and 159 conserved miRNAs as well as 86 novel miRNAs were successfully identified. Among the miRNAs identified, there were 90 miRNAs which showed differential expression patterns at different stages of fruit development and ripening. The target genes of these differential expressed (DE) miRNAs were involved in growth and development, transcription and regulation of transcription, metabolic processes, and etc. Interestingly, it was found that the expression level of mul-miR477 was increased with fruit ripening, and it can target the antisense lncRNA (Mul-ABCB19AS) of the ATP binding cassette (ABC) transporter B 19 gene (Mul-ABCB19). Our results showed that mul-miR477 can repress the expression of Mul-ABCB19AS and increase the expression of Mul-ABCB19, and it acted as a positive regulator participating anthocyanin accumulation through the regulatory network of mul-miR477—Mul-ABCB19AS—Mul-ABCB19.

Download Full-text