Healthcare-associated carbapenem-resistant OXA-72-producing Acinetobacter baumannii of the clonal complex CC79 colonizing migratory and captive aquatic birds in a Brazilian Zoo

2020 ◽  
Vol 726 ◽  
pp. 138232
Author(s):  
Ana Clara Narciso ◽  
Willames M.B.S. Martins ◽  
Luiz G.P. Almeida ◽  
Rodrigo Cayô ◽  
Stéfanie Vanessa Santos ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Aquatic Birds ◽  
Carbapenem Resistant ◽  
Healthcare Associated

scholarly journals Population Structure Analysis of Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates from Brazil Reveals Predominance of Clonal Complexes 1, 15, and 79

2016 ◽  
Vol 60 (4) ◽  
pp. 2545-2547 ◽  
Author(s):  
Carlos Henrique Camargo ◽  
Monique Ribeiro Tiba ◽  
Marta Regina Saes ◽  
Francielli Mahnic de Vasconcellos ◽  
Luis Fernando dos Santos ◽  
...  
Keyword(s):  
Population Structure ◽  
Acinetobacter Baumannii ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Population Structure Analysis

ABSTRACTThe population structure of 71 carbapenem-resistantAcinetobacter baumanniiclinical isolates from several hospitals in Brazil was investigated by ApaI pulsed-field gel electrophoresis,blaOXA-51-like subtyping, and multilocus sequence typing (Institute Pasteur scheme). In addition to the predominance of strains carryingblaOXA-23, we detected the presence ofblaOXA-72andblaOXA-231. We observed a predominance of clonal complex 1 (CC1), CC15, and CC79 and representative strains of the worldwide-disseminated international clone I.

scholarly journals Subtypes, resistance and virulence platforms in extended-drug resistant Acinetobacter baumannii Romanian isolates

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Irina Gheorghe ◽  
Ilda Czobor Barbu ◽  
Marius Surleac ◽  
Ionela Sârbu ◽  
Laura Ioana Popa ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Carbapenem Resistance ◽  
Control Measures ◽  
Drug Resistant ◽  
Infection Control Measures ◽  
Carbapenem Resistant ◽  
Ambulatory Patients ◽  
Severe Infections ◽  
Healthcare Associated ◽  
Sequence Types

AbstractAcinetobacter baumannii has emerged worldwide as a dominant pathogen in a broad range of severe infections, raising an acute need for efficient antibacterials. This is the first report on the resistome and virulome of 33 extended drug-resistant and carbapenem-resistant A. baumannii (XDR CRAB) strains isolated from hospitalized and ambulatory patients in Bucharest, Romania. A total of 33 isolates were collected and analyzed using phenotypic antibiotic susceptibility and conjugation assays, PCR, whole-genome sequencing (WGS), pulsed-field gel electrophoresis (PFGE) and MultiLocus Sequence Typing (MLST). All isolates were extensively drug-resistant (XDR), being susceptible only to colistin. The carbapenem resistance was attributed by PCR mainly to blaOXA-24 and blaOXA-23 genes. PFGE followed by MLST analysis demonstrated the presence of nine pulsotypes and six sequence types. WGS of seven XDR CRAB isolates from healthcare-associated infections demonstrated the high diversity of resistance genes repertoire, as well as of mobile genetic elements, carrying ARGs for aminoglycosides, sulphonamides and macrolides. Our data will facilitate the understanding of resistance, virulence and transmission features of XDR AB isolates from Romanian patients and might be able to contribute to the implementation of appropriate infection control measures and to develop new molecules with innovative mechanisms of action, able to fight effectively against these bugs, for limiting the spread and decreasing the infection rate and mortality.

Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

2020 ◽  
Author(s):  
Aliakbar Rezaei ◽  
Hossein Fazeli ◽  
Jamshid Faghri
Keyword(s):  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Carbapenem Resistance ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Carbapenem Resistant ◽  
Rapd Pcr ◽  
Relationship Of ◽  
Sequence Types ◽  
Respiratory Specimens

AbstractThis study investigated carbapenem resistance among Acinetobacter baumannii isolated from respiratory specimens. Epidemiological relationship of the isolates was also evaluated. In this study, 81 respiratory specimens of A. baumannii from AL Zahra Hospital were confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was performed by disc diffusion method. Carbapenem resistance genes were identified by PCR. The isolates were typed by RAPD-PCR and multilocus sequence typing (MLST) methods. All isolates were resistant to imipenem and 80 isolates to meropenem. Frequency of oxacillinase genes was as follows: blaOXA-23 gene was positive in 74 (91.3%), blaOXA-24 gene in 50 (61.7%) and blaOXA-58 was not found in any isolates. On the other hand 22 (27.2%) isolates contained blaIMP-1, 3 (3.7%) isolates contained blaIMP-2 gene, 5 (6.2%) isolates contained blaVIM-1, 4 (5%) isolates had blaVIM-2 and none of the isolates had blaSIM-1 gene. RAPD-PCR typing identified 16 different patterns, with one pattern being the most frequent one in 26 isolates. In MLST 6 different sequence types were identified, the most predominant being ST2 belonging to clonal complex 2. The results of this study showed high resistance to carbapenems as well as high abundance of oxacillinase genes.

scholarly journals Wide dissemination of OXA-23-producing carbapenem-resistant Acinetobacter baumannii clonal complex 22 in multiple cities of China

2010 ◽  
Vol 65 (4) ◽  
pp. 644-650 ◽  
Author(s):  
Yiqi Fu ◽  
Jianying Zhou ◽  
Hua Zhou ◽  
Qing Yang ◽  
Zeqing Wei ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Carbapenem Resistant ◽  
Wide Dissemination

scholarly journals Molecular dissection of Carbapenem-resistant Acinetobacter baumannii circulating in Indian hospitals using Whole Genome Sequencing

2021 ◽  
Author(s):  
Steffimol Rose ◽  
Varun Shamanna ◽  
Anthony Underwood ◽  
Geetha Nagaraj ◽  
Akshatha Prasanna ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Whole Genome Sequencing ◽  
Acinetobacter Baumannii ◽  
Genome Sequencing ◽  
Clonal Complex ◽  
Carbapenem Resistance ◽  
Evolutionary Divergence ◽  
Whole Genome ◽  
Northern India ◽  
Carbapenem Resistant

Objectives: Carbapenem-resistant Acinetobacter baumannii (CRAB) has acquired worldwide recognition as a serious nosocomial infection. It poses a concern to hospitalized patients because of the limited therapeutic options available. Thus, we investigated the molecular epidemiology and antibiotic resistance profiles of A. baumannii isolates in India. Materials and Methods: We characterized 306 retrospective A. baumannii clinical isolates collected from 18 centers across 10 states and 1 Union Territory of India between 2015 and 2019. Molecular epidemiology, and carbapenem resistance were studied by Whole Genome Sequencing. Results: A total of 105 different Sequence Types (STs) were identified including 48 reported STs and 57 Novel STs. 99 isolates were classified into Clonal Complex 451 (CC451) among which ST848 and ST1956 were the common STs. Carbapenemase resistance was confirmed in all the isolates with the presence of intrinsic bla OXA -51-like genes, and the acquired bla OXA-23 and bla NDM-1 genes. Conclusion: Most of the isolates were grouped under clonal complex 451. ST1053 caused an outbreak in Northern India during 2018 and 2019. Novel MLST alleles and STs were also detected, underlining an evolutionary divergence in India. The carbapenem-resistance was dominated by OXA-type carbapenemases and further surveillance of these carbapenem-resistant A. baumannii and antimicrobial stewardship should be strengthened.

scholarly journals Clinical Carbapenem-Resistant Acinetobacter baylyi Strain CoharboringblaSIM-1andblaOXA-23from China

2011 ◽  
Vol 55 (11) ◽  
pp. 5347-5349 ◽  
Author(s):  
Zhihui Zhou ◽  
Xiaoxing Du ◽  
Li Wang ◽  
Qing Yang ◽  
Yiqi Fu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Gene Cassette ◽  
Large Plasmid ◽  
Acinetobacter Baylyi ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTblaSIM-1andblaOXA-23were codetected in clinical carbapenem-resistantAcinetobacter baylyistrain NB09A30. Both of carbapenemase genes were located on a large plasmid (ca. 360 kb).blaSIM-1was found as a gene cassette inserted into a class 1 integron identical to that determined inAcinetobactersp. isolates from South Korea. The genetic structure ofblaOXA-23in NB09A30 was different from that in the prevalentAcinetobacter baumanniiof clonal complex 92 (CC92) from the same hospital.

scholarly journals Spread of Carbapenem-Resistant Acinetobacter baumannii Global Clone 2 in Asia and AbaR-Type Resistance Islands

2013 ◽  
Vol 57 (11) ◽  
pp. 5239-5246 ◽  
Author(s):  
Dae Hun Kim ◽  
Ji-Young Choi ◽  
Hae Won Kim ◽  
So Hyun Kim ◽  
Doo Ryeon Chung ◽  
...  
Keyword(s):  
Hong Kong ◽  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Carbapenem Resistance ◽  
Surveillance Study ◽  
Structural Variations ◽  
Content Type ◽  
Resistance Determinants ◽  
Carbapenem Resistant ◽  
Resistance Island

ABSTRACTIn this surveillance study, we identified the genotypes, carbapenem resistance determinants, and structural variations of AbaR-type resistance islands among carbapenem-resistantAcinetobacter baumannii(CRAB) isolates from nine Asian locales. Clonal complex 92 (CC92), corresponding to global clone 2 (GC2), was the most prevalent in most Asian locales (83/108 isolates; 76.9%). CC108, or GC1, was a predominant clone in India. OXA-23 oxacillinase was detected in CRAB isolates from most Asian locales except Taiwan.blaOXA-24was found in CRAB isolates from Taiwan. AbaR4-type resistance islands, which were divided into six subtypes, were identified in most CRAB isolates investigated. Five isolates from India, Malaysia, Singapore, and Hong Kong contained AbaR3-type resistance islands. Of these, three isolates harbored both AbaR3- and AbaR4-type resistance islands simultaneously. In this study, GC2 was revealed as a prevalent clone in most Asian locales, with the AbaR4-type resistance island predominant, with diverse variants. The significance of this study lies in identifying the spread of global clones of carbapenem-resistantA. baumanniiin Asia.

scholarly journals Identifying Risk Factors for Healthcare-Associated Infections Caused by Carbapenem-Resistant Acinetobacter baumannii in a Neonatal Intensive Care Unit

2018 ◽  
Vol 18 (1) ◽  
pp. 75 ◽  
Author(s):  
Amira M. Sultan ◽  
Wael A. Seliem
Keyword(s):  
Risk Factors ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Acinetobacter Baumannii ◽  
Neonatal Intensive Care ◽  
Control Group ◽  
Healthcare Associated Infections ◽  
Carbapenem Resistant ◽  
Healthcare Associated

Objectives: Acinetobacter baumannii is a causative pathogen of various healthcare-associated infections (HAIs) and is particularly prevalent in high-risk hospital settings. This study aimed to determine risk factors associated with HAIs caused by carbapenem-resistant A. baumannii (CRAB) in a neonatal intensive care unit (NICU). Methods: This prospective study was performed between January 2013 and June 2014 among NICU patients at the Mansoura University Children’s Hospital, Mansoura, Egypt. Neonates who developed HAIs due to CRAB were assigned to a case group, while those infected with carbapenem-sensitive A. baumannii (CSAB) were assigned to a control group. Results: Among the 124 neonates who developed A. baumannii-caused HAIs during the study period, 91 (73.4%) were caused by CRAB and 33 (26.6%) were caused by CSAB. Prematurity, premature rupture of the membranes (PROM), a previous stay in another hospital, prolonged NICU stay, the presence of invasive devices, previous exposure to carbapenems or aminoglycosides and prolonged antibiotic therapy before infection were significantly associated with CRAB-caused HAIs. A multivariate logistic regression analysis identified prematurity (adjusted odds ratio [aOR] = 25.3; P <0.01), mechanical ventilation (aOR = 18.9; P <0.01) and the previous use of carbapenems (aOR = 124.7; P <0.01) or aminoglycosides (aOR = 22.6; P = 0.04) to be independent risk factors for CRAB infections. Conclusion: Various risk factors were significantly associated with CRAB-caused HAIs among the studied NICU patients.

scholarly journals Carbapenem-Resistant Acinetobacter baumannii in US hospitals: diversification of circulating lineages and antimicrobial resistance

2021 ◽  
Author(s):  
Alina Vladimirovna Iovleva ◽  
Mustapha M Mustapha ◽  
Marissa P Griffith ◽  
Lauren Komarow ◽  
Courtney Luterbach ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acinetobacter Baumannii ◽  
The United States ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Carbapenem Resistant ◽  
Vulnerable Patients ◽  
The Us ◽  
Hospital Systems ◽  
Healthcare Associated

Carbapenem-resistant Acinetobacter baumannii (CRAb) are a major cause of healthcare-associated infections. CRAb are typically multidrug-resistant and infection is difficult to treat. Despite the urgent threat that CRAb pose, few systematic studies of CRAb clinical and molecular epidemiology have been conducted. The Study Network of Acinetobacter as a Carbapenem-Resistant Pathogen (SNAP) is designed to investigate the clinical characteristics and contemporary population structure of CRAb circulating in US hospital systems using whole genome sequencing (WGS). Analysis of the initial 120 SNAP patients from four US centers revealed that CRAb remain a significant threat to hospitalized patients, affecting the most vulnerable patients and resulting in 24% all-cause 30-day mortality. The majority of currently circulating isolates belonged to ST2Pas, a part of Clonal Complex 2 (CC2), which is the dominant drug-resistant lineage in the United States and Europe. We identified three distinct sub-lineages within CC2, which differed in their antibiotic resistance phenotypes and geographic distribution. Most concerning, colistin resistance (38%) and cefiderocol (10%) resistance were common within CC2 sub-lineage C (CC2C), where the majority of isolates belonged to ST2Pas/ST281Ox. Additionally, we identified a newly emergent lineage, ST499Pas that was the most common non-CC2 lineage in our study and had a more favorable drug susceptibility profile compared to CC2. Our findings suggest a shift within the CRAb population in the US during the past 10 years, and emphasize the importance of real-time surveillance and molecular epidemiology in studying CRAb dissemination and clinical impact.

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