scholarly journals Gut microbiota dynamics in travelers returning from India colonized with extended-spectrum cephalosporin-resistant Enterobacteriaceae: A longitudinal study

2019 ◽  
Vol 27 ◽  
pp. 72-80 ◽  
Author(s):  
João Pires ◽  
Julia G. Kraemer ◽  
Esther Kuenzli ◽  
Sara Kasraian ◽  
Regula Tinguely ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Gut Microbiota ◽  
Extended Spectrum

scholarly journals Draft Genome Sequences of Two Enterobacter cloacae subsp. cloacae Strains Isolated from Australian Hematology Patients with Bacteremia

2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Lex E. X. Leong ◽  
David Shaw ◽  
Lito Papanicolas ◽  
Diana Lagana ◽  
Ivan Bastian ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Draft Genome ◽  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Healthy Individuals ◽  
Genome Sequences ◽  
Content Type ◽  
Extended Spectrum ◽  
Genes Encoding

ABSTRACT Enterobacter cloacae is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two Enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases.

scholarly journals Longitudinal Study of Extended-Spectrum-β-Lactamase- and AmpC-Producing Enterobacteriaceae in Household Dogs

2015 ◽  
Vol 59 (6) ◽  
pp. 3117-3124 ◽  
Author(s):  
Valérie O. Baede ◽  
Jaap A. Wagenaar ◽  
Els M. Broens ◽  
Birgitta Duim ◽  
Wietske Dohmen ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Bacterial Species ◽  
Clinical Signs ◽  
Gene Families ◽  
Wild Type ◽  
Macconkey Agar ◽  
Content Type ◽  
Fecal Shedding ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

ABSTRACTA longitudinal study was performed to (i) investigate the continuity of shedding of extended-spectrum-beta-lactamase (ESBL)-producingEnterobacteriaceaein dogs without clinical signs, (ii) identify dominant plasmid-mediated ESBL genes, and (iii) quantify ESBL-producingEnterobacteriaceaein feces. Fecal samples from 38 dogs were collected monthly for 6 months. Additional samples were collected from 7 included dogs on a weekly basis for 6 weeks. Numbers of CFU per gram of feces for non-wild-typeEnterobacteriaceaewere determined by using MacConkey agar supplemented with 1 mg/liter cefotaxime (MCC), and those for totalEnterobacteriaceaewere determined by using MacConkey agar. Cefotaxime-resistant isolates were screened by PCR and sequence analysis for the presence ofblaCTX-M,blaCMY,blaSHV,blaOXA, andblaTEMgene families. Bacterial species were identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) analysis. PCR-negative isolates were tested by a double-disk synergy test for enhanced AmpC expression. A total of 259 samples were screened, and 126 samples were culture positive on MCC, resulting in 352 isolates, 327 of which wereEscherichia coli. Nine dogs were continuously positive during this study, and 6 dogs were continuously negative. Monthly or weekly shifts in fecal shedding were observed for 23 dogs. Genotyping showed a large variety of ESBL genes and gene combinations at single and multiple consecutive sampling moments. The ESBL genesblaCTX-M-1,blaCTX-M-14,blaCTX-M-15,blaSHV-12, andblaCMY-2were most frequently found. The mean number of CFU of non-wild-typeEnterobacteriaceaewas 6.11 × 108CFU/g feces. This study showed an abundance of ESBL-producingEnterobacteriaceaein dogs in the Netherlands, mostly in high concentrations. Fecal shedding was shown to be highly dynamic over time, which is important to consider when studying ESBL epidemiology.

scholarly journals Carriage of Enterobacteria Producing Extended-Spectrum β-Lactamases and Composition of the Gut Microbiota in an Amerindian Community

2015 ◽  
Vol 60 (1) ◽  
pp. 507-514 ◽  
Author(s):  
María José Gosalbes ◽  
Jorge F. Vázquez-Castellanos ◽  
Cécile Angebault ◽  
Paul-Louis Woerther ◽  
Etienne Ruppé ◽  
...  
Keyword(s):  
Risk Factors ◽  
Gut Microbiota ◽  
Individual Risk ◽  
Content Type ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Individual Risk Factors

ABSTRACTEpidemiological and individual risk factors for colonization by enterobacteria producing extended-spectrum beta-lactamases (E-ESBL) have been studied extensively, but whether such colonization is associated with significant changes in the composition of the rest of the microbiota is still unknown. To address this issue, we assessed in an isolated Amerindian Guianese community whether intestinal carriage of E-ESBL was associated with specificities in gut microbiota using metagenomic and metatranscriptomic approaches. While the richness of taxa of the active microbiota of carriers was similar to that of noncarriers, the taxa were less homogeneous. In addition, species of four genera,Desulfovibrio,Oscillospira,Parabacteroides, andCoprococcus, were significantly more abundant in the active microbiota of noncarriers than in the active microbiota of carriers, whereas such was the case only for species ofDesulfovibrioandOscillospirain the total microbiota. Differential genera in noncarrier microbiota could either be associated with resistance to colonization or be the consequence of the colonization by E-ESBL.

Role of gut microbiota in travel-related acquisition of extended spectrum β-lactamase-producing Enterobacteriaceae

2021 ◽  
Author(s):  
Ye Peng ◽  
Suisha Liang ◽  
Kanchana Poonsuk ◽  
Hilda On ◽  
Sze Wang Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Random Forest ◽  
Gut Microbiota ◽  
Area Under The Curve ◽  
International Travel ◽  
Resistant Bacteria ◽  
Extended Spectrum ◽  
Related Risk ◽  
Associated Risk Factors

Abstract Background International travel could facilitate the spread of antimicrobial-resistant bacteria including extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E). Previous studies, which attempted to understand the role of gut microbiota in the acquisition of antimicrobial resistant bacteria during international travels, are limited to western travelers. Methods We established a prospective cohort of 90 Hong Kong travelers to investigate gut microbiota determinants and associated risk factors for the acquisition of ESBL-E. Baseline characteristics and travel-associated risk factors were gathered through questionnaires. Fecal samples were collected in 3–4 days before and after travel. Antimicrobial susceptibility of ESBL-E isolates was tested, and gut microbiota were profiled by 16S rDNA amplicon sequencing. Non-parametric tests were used to detect potential associations, and logistic regression models were used to quantify the associations. Random forest models were constructed to identify microbial predictors for ESBL-E acquisition. Results 49 (54.4%) participants were tested negative for ESBL-E colonization before travel and were followed up after travel. A total of 60 ESBL-E isolates were cultured from 20 (40.8%) participants. Having low Actinobacteria richness and low abundance of short-chain fatty acid-producing bacteria in the gut microbiota before travel increased the risk of acquiring ESBL-E and the risk can be further exacerbated by eating raw seafood during travel. Besides, post-travel ESBL-E positive participants had increased abundances of several opportunistic pathogens such as Staphylococcus, Enterococcus, Escherichia/Shigella and Klebsiella. The random forest model integrating pre-travel microbiota and the identified travel-related risk factor could predict ESBL-E acquisition with an area under the curve of 75.4% (95% confidence interval: 57.9%–93.0%). Conclusions In this study, we identified both travel-related risk factors and microbiota predictors for the risk of ESBL-E acquisition. Our results provide foundational knowledge for future developments of microbiota-based interventions to prevent ESBL-E acquisition during international travels.

scholarly journals Patterns of seasonality and group membership characterize the gut microbiota in a longitudinal study of wild Verreaux's sifakas (Propithecus verreauxi)

2017 ◽  
Vol 7 (15) ◽  
pp. 5732-5745 ◽  
Author(s):  
Andrea Springer ◽  
Claudia Fichtel ◽  
Gabriel A. Al-Ghalith ◽  
Flávia Koch ◽  
Katherine R. Amato ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Gut Microbiota ◽  
Group Membership ◽  
Propithecus Verreauxi

scholarly journals Molecular analysis of blaSHV, blaTEM, and blaCTX-M in extended-spectrum β-lactamase producing Enterobacteriaceae recovered from fecal specimens of animals

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245126
Author(s):  
Hasan Ejaz ◽  
Sonia Younas ◽  
Khalid O. A. Abosalif ◽  
Kashaf Junaid ◽  
Badr Alzahrani ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Gut Microbiota ◽  
Domestic Animals ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Extensive Drug Resistance ◽  
Global Threat ◽  
Resistance Spectrum ◽  
Prevalent Species

Colonization of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as animal gut microbiota is a substantial global threat. This study aimed to determine the molecular characterization of blaSHV, blaTEM, and blaCTX-M variants in animals, as well as to evaluate the antimicrobial resistance conferred by these genes. We prospectively analyzed 1273 fecal specimens of farm and domestic animals for the isolation of enterobacteria that had the ESBL phenotype by using biochemical methods. The extracted genes were amplified by polymerase chain reaction and sequenced for the characterization of blaSHV, blaTEM, and blaCTX-M variants. The drug-resistance spectrum and hierarchical clusters were analyzed against 19 antibacterial agents. Out of 245 (19.2%) ESBL enterobacteria, 180 (75.5%) Escherichia coli and 34 (13.9%) Klebsiella pneumoniae were prevalent species. A total of 73.9% blaCTX-M, 26.1% blaTEM, and 14.2% blaSHV were found among the enterobacteria; however, their association with farm or domestic animals was not statistically significant. The distribution of bla gene variants showed the highest number of blaCTX-M-1 (133; 54.3%), followed by blaCTX-M-15 (28; 11.4%), blaTEM-52 (40; 16.3%), and blaSHV-12 (22; 9%). In addition, 84.5% of the enterobacteria had the integrons intI1. We observed ±100% enterobacteria resistant to cephalosporin, 7 (2.9%) to colistin (minimum inhibitory concentration breakpoint ≥4 μg/mL), 9 (3.7%) to piperacillin-tazobactam, 11 (4.5%) to imipenem, 14 (5.7%) to meropenem, and 18 (7.3%) to cefoperazone-sulbactam, without statistically significant association. Animal gut microbiota contain a considerable number of blaCTX-M, blaTEM, blaSHV, and integrons, which are a potential source of acquired extensive drug resistance in human strains and leaves fewer therapeutic substitutes.

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