scholarly journals Metabogenomic analysis to functionally annotate the regulatory role of long non-coding RNAs in the liver of cows with different nutrient partitioning phenotype

Genomics ◽  
2021 ◽  
Author(s):  
Wietje Nolte ◽  
Rosemarie Weikard ◽  
Elke Albrecht ◽  
Harald M. Hammon ◽  
Christa Kühn
Keyword(s):  
Regulatory Role ◽  
Nutrient Partitioning ◽  
Non Coding Rnas

scholarly journals Identification and characterization of early Fusarium wilt responsive mRNAs and long non-coding RNAs in banana root using high-throughput sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chunzhen Cheng ◽  
Fan Liu ◽  
Na Tian ◽  
Raphael Anue Mensah ◽  
Xueli Sun ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Acid Metabolism ◽  
Function Analysis ◽  
Regulatory Role ◽  
Novel Genes ◽  
Root Transcriptome ◽  
Qrt Pcr ◽  
Non Coding Rnas ◽  
Infection Stage

AbstractFusarium wilt disease, caused by Fusarium oxysporum f.sp. cubense (Foc), has been recognized as the most devastating disease to banana. The regulatory role of long non-coding RNAs (lncRNAs) in plant defense has been verified in many plant species. However, the understanding of their role during early FocTR4 (Foc tropical race 4) infection stage is very limited. In this study, lncRNA sequencing was used to reveal banana root transcriptome profile changes during early FocTR4 infection stages. Quantitative real time PCR (qRT-PCR) was performed to confirm the expression of eight differentially expressed (DE) lncRNAs (DELs) and their predicted target genes (DETs), and three DE genes (DEGs). Totally, 12,109 lncRNAs, 36,519 mRNAs and 2642 novel genes were obtained, of which 1398 (including 78 DELs, 1220 DE known genes and 100 DE novel genes) were identified as FocTR4 responsive DE transcripts. Gene function analysis revealed that most DEGs were involved in biosynthesis of secondary metabolites, plant–pathogen interaction, plant hormone signal transduction, phenylalanine metabolism, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and so on. Coincidently, many DETs have been identified as DEGs in previous transcriptome studies. Moreover, many DETs were found to be involved in ribosome, oxidative phosphorylation, lipoic acid metabolism, ubiquitin mediated proteolysis, N-glycan biosynthesis, protein processing in endoplasmic reticulum and DNA damage response pathways. QRT-PCR result showed the expression patterns of the selected transcripts were mostly consistent with our lncRNA sequencing data. Our present study showed the regulatory role of lncRNAs on known biotic and abiotic stress responsive genes and some new-found FocTR4 responsive genes, which can provide new insights into FocTR4-induced changes in the banana root transcriptome during the early pathogen infection stage.

scholarly journals MicroRNAs in the tumour microenvironment: big role for small players

2013 ◽  
Vol 20 (5) ◽  
pp. R257-R267 ◽  
Author(s):  
Patsy Soon ◽  
Hippokratis Kiaris
Keyword(s):  
Clinical Characteristics ◽  
Experimental Studies ◽  
Anticancer Therapy ◽  
Tumour Microenvironment ◽  
Regulatory Role ◽  
Tumour Stroma ◽  
Physiological Processes ◽  
Non Coding Rnas

MicroRNAs (miRNAs) represent a class of small non-coding RNAs with an important regulatory role in various physiological processes as well as in several pathologies including cancers. It is noteworthy that recent evidence suggests that the regulatory role of miRNAs during carcinogenesis is not limited to the cancer cells but they are also implicated in the activation of tumour stroma and its transition into a cancer-associated state. Results from experimental studies involving cells culturedin vitroand mice bearing experimental tumours, corroborated by profiling of clinical cancers for miRNA expression, underline this role and identify miRNAs as a potent regulator of the crosstalk between cancer and stroma cells. Considering the fundamental role of the tumour microenvironment in determining both the clinical characteristics of the disease and the efficacy of anticancer therapy, miRNAs emerge as an attractive target bearing important prognostic and therapeutic significance during carcinogenesis. In this article, we will review the available results that underline the role of miRNAs in tumour stroma biology and emphasise their potential value as tools for the management of the disease.

scholarly journals Insights into the Regulatory Role of Non-coding RNAs in Cancer Metabolism

2016 ◽  
Vol 7 ◽  
Author(s):  
Fredy O. Beltrán-Anaya ◽  
Alberto Cedro-Tanda ◽  
Alfredo Hidalgo-Miranda ◽  
Sandra L. Romero-Cordoba
Keyword(s):  
Cancer Metabolism ◽  
Regulatory Role ◽  
Non Coding Rnas

scholarly journals The Regulatory Role of Non-coding RNAs on Programmed Cell Death Four in Inflammation and Cancer

2019 ◽  
Vol 9 ◽  
Author(s):  
Mengxiang Zhao ◽  
Nisha Zhu ◽  
Fengyao Hao ◽  
Yuxian Song ◽  
Zhiyong Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Regulatory Role ◽  
Non Coding Rnas

scholarly journals Differentially expressed microRNAs under imidacloprid exposure and identification in Sitobion avenae

2019 ◽  
Author(s):  
Baizhong Zhang ◽  
Jun-Jie Liu ◽  
Liu-yang Lu ◽  
Lan-Fen Xie ◽  
Xi-Ling Chen ◽  
...  
Keyword(s):  
Target Genes ◽  
De Novo ◽  
Sitobion Avenae ◽  
Differentially Expressed ◽  
Regulatory Role ◽  
Sequencing Technology ◽  
Quantitative Expression ◽  
Non Coding Rnas ◽  
Putative Transcript

Abstract BACKGROUD MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate the expression of target genes, especially regulation or metabolism of endogenous or xenobiotic compounds. RESULTS The de novo assembly of the transcriptomes was obtained through Illumina short-read sequencing technology in Sitobion avenae. 57 miRNAs, of which 36 were known and 21 were novel were identified. Quantitative expression levels of miRNA showed that the expression of 5 miRNAs were significant up-regulation, and the expression of 11 miRNAs were significant down-regulation in the nymph of S. avenae treated by imidacloprid compared to the control, respectively. The putative transcript target genes in S. avenae that could be regulated by these miRNAs were also carried out. The potential functions of these miRNAs in the regulation of genes involved in the metabolism, regulatory or detoxification of S. avenae were clarified based on Gene Ontology and KEGG pathway. The effects of these miRNAs identified api-miR-1000, api-miR-316, and api-miR-iab-4 on susceptibility of S. avenae to imidacloprid was determined. Modulation of the abundance of api-miR-1000, api-miR-316, and api-miR-iab-4 through the addition of inhibitors of api-miR-1000, api-miR-316, and api-miR-iab-4 to the artificial diet significantly altered the susceptibility of S. avenae to imidacloprid, which further proved that the regulatory role of these miRNAs in regulation or metabolism of insecticides. CONCLUSION It suggested that differentially expressed microRNAs under the stress of imidacloprid could play critical regulatory role in the resistance of S. avenae to imidacloprid.

scholarly journals RNA sequencing and functional analysis implicate the regulatory role of long non-coding RNAs in tomato fruit ripening

2015 ◽  
Vol 66 (15) ◽  
pp. 4483-4495 ◽  
Author(s):  
Benzhong Zhu ◽  
Yongfang Yang ◽  
Ran Li ◽  
Daqi Fu ◽  
Liwei Wen ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Rna Sequencing ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Regulatory Role ◽  
Tomato Fruit Ripening ◽  
Non Coding Rnas
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