scholarly journals Identification and characterization of early Fusarium wilt responsive mRNAs and long non-coding RNAs in banana root using high-throughput sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chunzhen Cheng ◽  
Fan Liu ◽  
Na Tian ◽  
Raphael Anue Mensah ◽  
Xueli Sun ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Acid Metabolism ◽  
Function Analysis ◽  
Regulatory Role ◽  
Novel Genes ◽  
Root Transcriptome ◽  
Qrt Pcr ◽  
Non Coding Rnas ◽  
Infection Stage

AbstractFusarium wilt disease, caused by Fusarium oxysporum f.sp. cubense (Foc), has been recognized as the most devastating disease to banana. The regulatory role of long non-coding RNAs (lncRNAs) in plant defense has been verified in many plant species. However, the understanding of their role during early FocTR4 (Foc tropical race 4) infection stage is very limited. In this study, lncRNA sequencing was used to reveal banana root transcriptome profile changes during early FocTR4 infection stages. Quantitative real time PCR (qRT-PCR) was performed to confirm the expression of eight differentially expressed (DE) lncRNAs (DELs) and their predicted target genes (DETs), and three DE genes (DEGs). Totally, 12,109 lncRNAs, 36,519 mRNAs and 2642 novel genes were obtained, of which 1398 (including 78 DELs, 1220 DE known genes and 100 DE novel genes) were identified as FocTR4 responsive DE transcripts. Gene function analysis revealed that most DEGs were involved in biosynthesis of secondary metabolites, plant–pathogen interaction, plant hormone signal transduction, phenylalanine metabolism, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and so on. Coincidently, many DETs have been identified as DEGs in previous transcriptome studies. Moreover, many DETs were found to be involved in ribosome, oxidative phosphorylation, lipoic acid metabolism, ubiquitin mediated proteolysis, N-glycan biosynthesis, protein processing in endoplasmic reticulum and DNA damage response pathways. QRT-PCR result showed the expression patterns of the selected transcripts were mostly consistent with our lncRNA sequencing data. Our present study showed the regulatory role of lncRNAs on known biotic and abiotic stress responsive genes and some new-found FocTR4 responsive genes, which can provide new insights into FocTR4-induced changes in the banana root transcriptome during the early pathogen infection stage.

scholarly journals MicroRNAs in the tumour microenvironment: big role for small players

2013 ◽  
Vol 20 (5) ◽  
pp. R257-R267 ◽  
Author(s):  
Patsy Soon ◽  
Hippokratis Kiaris
Keyword(s):  
Clinical Characteristics ◽  
Experimental Studies ◽  
Anticancer Therapy ◽  
Tumour Microenvironment ◽  
Regulatory Role ◽  
Tumour Stroma ◽  
Physiological Processes ◽  
Non Coding Rnas

MicroRNAs (miRNAs) represent a class of small non-coding RNAs with an important regulatory role in various physiological processes as well as in several pathologies including cancers. It is noteworthy that recent evidence suggests that the regulatory role of miRNAs during carcinogenesis is not limited to the cancer cells but they are also implicated in the activation of tumour stroma and its transition into a cancer-associated state. Results from experimental studies involving cells culturedin vitroand mice bearing experimental tumours, corroborated by profiling of clinical cancers for miRNA expression, underline this role and identify miRNAs as a potent regulator of the crosstalk between cancer and stroma cells. Considering the fundamental role of the tumour microenvironment in determining both the clinical characteristics of the disease and the efficacy of anticancer therapy, miRNAs emerge as an attractive target bearing important prognostic and therapeutic significance during carcinogenesis. In this article, we will review the available results that underline the role of miRNAs in tumour stroma biology and emphasise their potential value as tools for the management of the disease.

scholarly journals Role of the DksA-Like Protein in the Pathogenesis and Diverse Metabolic Activity of Campylobacter jejuni

2008 ◽  
Vol 190 (13) ◽  
pp. 4512-4520 ◽  
Author(s):  
Jiae Yun ◽  
Byeonghwa Jeon ◽  
Yi-Wen Barton ◽  
Paul Plummer ◽  
Qijing Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Campylobacter Jejuni ◽  
Pathogenic Bacteria ◽  
Acid Metabolism ◽  
Acid Synthesis ◽  
Regulatory Role ◽  
Intestinal Cells ◽  
Amino Acid Synthesis ◽  
Metabolic Reactions

ABSTRACT DksA is well known for its regulatory role in the transcription of rRNA and genes involved in amino acid synthesis in many bacteria. DksA has also been reported to control expression of virulence genes in pathogenic bacteria. Here, we elucidated the roles of a DksA-like protein (CJJ81176_0160, Cj0125c) in the pathogenesis of Campylobacter jejuni. As in other bacteria, transcription of stable RNA was repressed by the DksA-like protein under stress conditions in C. jejuni. Transcriptomic and proteomic analyses of C. jejuni 81-176 and an isogenic mutant lacking the DksA-like protein showed differential expression of many genes involved in amino acid metabolism, iron-related metabolism, and other metabolic reactions. Also, the C. jejuni DksA-like protein mutant exhibited a decreased ability to invade intestinal cells and induce release of interleukin-8 from intestinal cells. These results suggest that the DksA-like protein plays an important regulatory role in diverse metabolic events and the virulence of C. jejuni.

scholarly journals Insights into the Regulatory Role of Non-coding RNAs in Cancer Metabolism

2016 ◽  
Vol 7 ◽  
Author(s):  
Fredy O. Beltrán-Anaya ◽  
Alberto Cedro-Tanda ◽  
Alfredo Hidalgo-Miranda ◽  
Sandra L. Romero-Cordoba
Keyword(s):  
Cancer Metabolism ◽  
Regulatory Role ◽  
Non Coding Rnas

scholarly journals P.038 Investigating the role of long non-coding RNAs in glioblastoma multiforme

2018 ◽  
Vol 45 (s2) ◽  
pp. S26-S26
Author(s):  
A Lebel ◽  
V Charest ◽  
P Whitlock ◽  
D Charest ◽  
P Morin
Keyword(s):  
Survival Rate ◽  
Glioblastoma Multiforme ◽  
Brain Tumors ◽  
Malignant Gliomas ◽  
Standard Of Care ◽  
Qrt Pcr ◽  
Resistance Modulation ◽  
Non Coding Rnas ◽  
One Year

Background: Malignant gliomas are the most common and deadly brain tumors. Mean survival rate for a patient diagnosed with a glioblastoma multiforme (GBM) remains slightly over one year. Standard of care consists of treatment with temozolomide (TMZ) and radiotherapy. Recent work has highlighted functions of long non-coding RNAs (lncRNAs) in GBM progression and TMZ response even though the information regarding these newly discovered molecules is sparse. The overarching objective of this project was thus to assess the expression of select lncRNAs in GBM tumor samples and in models of TMZ resistance. Methods: A qRT-PCR-based approach was undertaken to measure six lncRNAs in 19 primary GBM samples, four GBM cell lines and in-house developed TMZ-resistant GBM cells. Results: Elevated levels of Hotair and H19 were observed in primary GBM tumors while decreased expression of MEG3 was recorded in the same samples. Interestingly, levels of PANDA increased 3.4-fold in GBM cells resistant to TMZ when compared with their sensitive counterparts. Conclusions: Overall, this work provides evidence of lncRNA deregulation in GBM tumors and reveals a previously unexplored lncRNA potentially involved in TMZ resistance. Modulation of lncRNA targets via RNAi-mediated approaches is envisioned to clarify their function and to strengthen their position as therapeutic options in GBMs.

scholarly journals The Regulatory Role of Non-coding RNAs on Programmed Cell Death Four in Inflammation and Cancer

2019 ◽  
Vol 9 ◽  
Author(s):  
Mengxiang Zhao ◽  
Nisha Zhu ◽  
Fengyao Hao ◽  
Yuxian Song ◽  
Zhiyong Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Regulatory Role ◽  
Non Coding Rnas

scholarly journals LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhenxing Sun ◽  
Naili Wei ◽  
Shenglian Yao ◽  
Guihuai Wang ◽  
Yaxing Sun ◽  
...  
Keyword(s):  
Glioma Cell ◽  
Caspase 3 ◽  
Recent Literature ◽  
Subcellular Fractionation ◽  
Luciferase Reporter ◽  
Biological Functions ◽  
Positive Regulation ◽  
Qrt Pcr ◽  
Non Coding Rnas

Abstract Background Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated. Methods qRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p. Results LINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK. Conclusion LINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma.

Sign in / Sign up

Export Citation Format

Share Document