The relationship between post-parturient fatty liver and blood composition in dairy cows

1983 ◽  
Vol 101 (2) ◽  
pp. 473-480 ◽  
Author(s):  
I. M. Reid ◽  
G. J. Rowlands ◽  
A. M. Dew ◽  
R. A. Collins ◽  
C. J. Roberts ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Aspartate Aminotransferase ◽  
Blood Composition ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Esterified Fatty Acids ◽  
Aspartate Amino Transferase ◽  
The Relationship

SUMMARYThe blood composition of 45 cows with more than 20% fat in the liver (moderate fatty liver) was compared with that of 83 cows with less than 20% fat in the liver (mild fatty liver). The cows were sampled at 1 week after calving and were from three Friesian and one Guernsey herds. Blood concentrations of non-esterified fatty acids, bilirubin and aspartate amino-transferase were significantly elevated in cows with more than 20% in the liver whereas concentrations of glucose, cholesterol, albumin and magnesium were all significantly reduced. Blood samples from cows in two of the herds were analysed for ammonia, insulin and D(-)3-hydroxybutyrate. Cows with moderate fatty liver in these two herds had significantly lower insulin concentrations and higher D(-)3-hydroxybutyrate concentrations than those with mild fatty liver. Using an equation based on blood concentrations of non-esterified fatty acids, glucose and aspartate aminotransferase it was possible to assign correctly three out of four cows to either the mild or moderate fatty liver groups.

scholarly journals Investigation of omentin-1 and metabolic parameters in periparturient cows

2021 ◽  
Vol 72 (1) ◽  
pp. 2733
Author(s):  
S AYGORMEZ ◽  
E ATAKISI
Keyword(s):  
Fatty Acids ◽  
Energy Metabolism ◽  
Dairy Cows ◽  
Negative Correlation ◽  
Serum Glucose ◽  
Blood Samples ◽  
Positive Correlation ◽  
Periparturient Period ◽  
Esterified Fatty Acids ◽  
Periparturient Cows

We aimed to determine the level of omentin-1 hormone and other in periparturient period of dairy cows. It was also aimed to determine whether there is a correlation between omentin-1, glucose, Non-esterified fatty acids (NEFA), triglycerides (TG) and β-hydroxybutyrate (BHB). Blood samples were taken a month before parturition (PreP30), two weeks before parturition (PreP14), at parturition (P), two weeks after parturition (PostP14) and a month after parturition (PostP30). Concentrations of serum omentin-1 at P, serum glucose at P, PostP14, and PostP30, serum NEFA at P, serum TG PreP30 and PreP14, and serum BHB at P were statistically significantly higher than at other times. A positive correlation was observed between omentin-1 and glucose, NEFA and BHB, glucose and NEFA and BHB, and NEFA and BHB. A negative correlation was found between TG and omentin-1, glucose, NEFA and BHB. In conclusion, findings of the present study shows that omentin-1 may play an important role in the periparturient period. A positive correlation of omentin-1 with energy fuels NEFA, glucose, and BHB suggests that omentin-1 plays a role in energy metabolism like other adipokines. The fact that omentin-1 levels increase during delivery, when the fetus needs energy most, supports this hypothesis.

The effects of non-esterified fatty acids and β-hydroxybutyrate on the hepatic CYP2E1 in cows with clinical ketosis

2019 ◽  
Vol 86 (1) ◽  
pp. 68-72
Author(s):  
Zhicheng Peng ◽  
Xiaobing Li ◽  
Zhe Wang ◽  
Guowen Liu ◽  
Xinwei Li
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Dairy Cows ◽  
Blood Concentrations ◽  
Cytochrome P4502e1 ◽  
Esterified Fatty Acids ◽  
Cyp2e1 Expression ◽  
Expression And Activity

AbstractDairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis exhibited a low blood concentration of glucose but high concentrations of NEFA and BHB. Hepatic mRNA, protein expression, and activity of CYP2E1 were significantly higher in cows with clinical ketosis than in control cows. In vitro, both NEFA and BHB treatment markedly up-regulated the mRNA and protein expressions as well as activity of CYP2E1 in cow hepatocytes. Taken together, these results indicate that high levels of NEFA and BHB significantly up-regulate the expression and activity of hepatic CYP2E1, and may be influential in the induction of oxidative stress in cows with clinical ketosis.

Effects of Yerba Mate (Ilex paraguariensis) supplementation on the productive performance of dairy cows during mid-lactation

2010 ◽  
Vol 50 (6) ◽  
pp. 339 ◽  
Author(s):  
Pietro Celi ◽  
Herman W. Raadsma
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Ilex Paraguariensis ◽  
Yerba Mate ◽  
Reactive Oxygen Metabolites ◽  
Blood Samples ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Esterified Fatty Acids ◽  
Oxygen Metabolites

Yerba Mate (Ilex paraguariensis), a tea known for its high antioxidant content, was fed (250 g/cow.day) to 8 of 16 Holstein cows for 6 weeks to assess its effect on their performance. Cows were weighed and blood samples were taken on Weeks 0, 3 and 6. Blood samples were centrifuged and plasma was analysed for reactive oxygen metabolites, biological antioxidant potential, advanced oxidation protein products and non-esterified fatty acids. Cows were milked two times daily and milk yields were recorded daily for individual cows. On Weeks 0 and 6, individual milk samples were collected from two consecutive milkings, composited, and analysed for somatic cell counts, fat and true protein concentrations. Plasma concentrations of reactive oxygen metabolites, biological antioxidant potential and non-esterified fatty acids were not affected by Yerba Mate supplementation. Similarly, no effect of Yerba Mate supplementation was noted on milk fat and protein content and on somatic cell counts. This study indicates that supplementation of dairy cows’ diet with Yerba Mate during mid lactation seems to improve milk yield when cows are fed with maize silage; however, even if the effect on milk yield was significant it was quite small and needs to be validated with further studies. Cows’ oxidative status was not affected by Yerba Mate supplementation indicating that the effect of Yerba Mate on their productive performances is not mediated by changes in redox status.

scholarly journals Biochemical Profile and Productive Performance in Dairy Cows with Lameness During Postpartum Period

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Renan Braga Paiano ◽  
Daniela Becker Birgel ◽  
Rüdiger Daniel Ollhoff ◽  
Eduardo Harry Birgel Junior
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Production ◽  
Body Condition ◽  
Body Condition Score ◽  
Biochemical Profile ◽  
Productive Performance ◽  
Blood Samples ◽  
Esterified Fatty Acids ◽  
Condition Score

Background: Lameness in dairy cows is classified as a gait change caused by multifactorial process. The phase of the peripartum causes intense physiological changes for the adaptation of late gestation and onset of lactation. The aim of this study was to characterize the changes in the biochemical profile and productive performance in dairy cows with lameness during postpartum period.Materials, Methods & Results: This study was conducted at the University of São Paulo farm, in Pirassununga, São Paulo State, Brazil, from January to March 2017. A total, of 48 multiparous (2 to 3 lactations and 3-4 years old) dairy cows, that had the milk production of 9,200 kg/ dairy cow in a period of 305 days in the previous lactation, were included in the study. All cows were managed under the same conditions and nutritional regimen. Evaluation of body condition score was performed by a single person on -18, -12, -8, -5, and -2 days before parturition, at parturition, and on days 1, 7, 14, 21, 30, 45 and 60 after parturition. Milk production was recorded on days 7, 14, 21, 30, 45 and 60 after parturition and saved in the software program. Blood samples were performed on -18, -12, -8, -5, and -2 days before parturition, at parturition, and on days 1, 7, 14, 21, 30, 45 and 60 after parturition. Blood samples were assayed for albumin, calcium, cholesterol, triglycerides, non-esterified fatty acids, β-hydroxybutyrate, urea, creatinine, gamma-glutamil-transferase and total protein concentrations. Dairy cows were divided into a lame group (11) and normal group (37) based on locomotion score from parturition to seven days postpartum. Lame cows was classified if their score was > 2, and normal cows was classified if their score was ≤ 2 and free of any disease. Dairy cows that suffer by any health disorder other than lameness were excluded from this study. Cow diagnosed with lameness outside the diagnostic period were excluded from this study. The averages of the milk production, body condition score and biochemical profile were compared with the Tukey’s test. Lame cows showed lower (P < 0.05) concentrations of albumin (on days -18 -12, -8, -5, -2 relative to parturition, at parturition, and on days 7, 14, 21, 45 and 60 after parturition) calcium (on days -18, -12, -8, -5 and -2 before parturition, and on days 7, 14, 21, 45 and 60 after parturition), cholesterol (on days -12, -8, -5 before parturition and on days 7, 14, 21, 30, 45 and 60 after parturition) than normal cows. Cow with lameness showed higher (P < 0.05) concentration of triglyceride (on days 7, 14, 21, 30, 45 and 60 after parturition), non-esterified fatty acids (on days 1, 7, 14, 21, 30, 45 and 60 after parturition) and β-hydroxybutyrate (on days -12, -5 and -2 before parturition, at parturition, and on days 1, 14, 21 and 30 after parturition) than normal group. Cows with lameness presented higher (P < 0.05) values of body condition score on days -18, -12 and -8 before parturition, and lower (P < 0.05) values on days 7, 14, 21, 30, 45 and 60 after parturition than normal cows. Milk production was lower (P < 0.05) for cows with lameness (on days 7, 14, 21, 30, 45 and 60 after parturition) than normal cows.Discussion: The overall prevalence of lameness in the evaluated period was 22.92% (11/48). Among lame cows, 07 presented laminitis, 02 had interdigital hyperplasia and 02 had sole ulcer. Our findings prove that the biochemical profile and productivity of dairy cows during the peripartum and postpartum period were affected by lameness at the early lactation. 

Evaluation of fructosamine as a new biomarker for diagnosis of hepatic lipidosis in dairy cows

2015 ◽  
Vol 55 (8) ◽  
pp. 1005 ◽  
Author(s):  
Masoud Mostafavi ◽  
Hesam A. Seifi ◽  
Mehrdad Mohri ◽  
Abdullah Jamshidi
Keyword(s):  
Fatty Acids ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Likelihood Ratio ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Serum Total Bilirubin ◽  
Hepatic Lipidosis ◽  
Esterified Fatty Acids ◽  
Serum Fructosamine

An abattoir-based cohort study using liver and serum specimens from Holstein dairy cows (452 healthy and 54 fatty liver cases) was conducted. Serum fructosamine and other biochemical parameters and fat content of liver specimens were measured. There were significant negative correlations of fructosamine with hepatic lipid content (P = 0.001), and serum total bilirubin (P = 003) and significant positive correlation with glucose (P < 0.02), albumin (P < 0.0001) and cholesterol (P < 0.0001) within normal cows. In fatty liver-affected cows significant positive correlations were seen between fructosamine and cholesterol (P < 0.0001) and albumin (P < 0.0001). In addition fructosamine had a significant negative correlation with the ratio of non-esterified fatty acids to cholesterol in both normal (P < 0.0001) and fatty liver-affected cows (P = 0.006). We found that serum fructosamine concentration was lower (P < 0.001) in cows suffering from fatty liver. The area under the receiver operating characteristic curve of fructosamine for diagnosis of fatty liver was 67.6. Optimum fructosamine cut-point based on the maximum total of sensitivity (SE) and specificity (SP) was <213 µmol/L (SE = 71%; SP = 65). Cows with serum fructosamine concentrations below 213 µmol/L were 4.5 times more likely to have hepatic lipidosis (odds ratio = 4.5; 95% CI = 2.4–8.6; P < 0.0001). We found that fructosamine showed a combination of high SE, SP, which was greater than aspartate aminotransferase, non-esterified fatty acids, β-hydroxybutyrate, total cholesterol, bile acids and bilirubin. In addition, fructosamine showed a positive likelihood ratio of 2.0, and negative likelihood ratio of 0.45. In conclusion, the present results indicate that fructosamine measurement could improve the diagnosis of fatty liver in dairy cows.

Effects of growth hormone-releasing factors and cottonseed meal on hormones and metabolites in plasma from lambs fed lucerne chaff ad libitum

1994 ◽  
Vol 45 (6) ◽  
pp. 1125 ◽  
Author(s):  
RD Sainz ◽  
BJ Hosking ◽  
FJ Hart ◽  
GSG Spencer
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Growth Factor ◽  
Cottonseed Meal ◽  
Metabolic Effects ◽  
Insulin Like Growth Factor ◽  
Plasma Urea ◽  
Blood Samples ◽  
Esterified Fatty Acids ◽  
Ad Libitum

This study was part of an experiment on the effects of cottonseed meal (CSM) and growth hormone-releasing factor (GRF) on growth in lambs fed lucerne chaff. Forty-eight crossbred lambs were fed lucerne chaff ad libitum, alone or with a cottonseed meal supplement (CSM; 300 g/day). Eight lambs from each group were injected twice daily with recombinant or synthetic GRF (rGRF and sGRF respectively; 30 8g per kg body weight/day) or excipient only for 28 or 30 days. Jugular blood samples were obtained on days 0, 7, 14, 21 and 28. On day 29, blood samples were taken from selected lambs (n = 2/group) at 0, 20, 40, 60, 80, 100, 120, 160 and 240 min after injection. Supplementation of lucerne chaff with CSM generally increased glucose and urea concentrations in plasma, reflecting improved energy and protein status. However, concentrations of growth hormone, insulin-like growth factor-1 and insulin were unaffected by CSM, as were plasma proteins, triacylglycerols and non-esterified fatty acids. Injection of rGRF and sGRF stimulated secretion of growth hormone (GH), insulin-like growth factor-1 (IGF-1) and insulin. Peak GH concentrations (20 min) following injection of GRF appeared to be higher in lambs fed CSM than in those receiving lucerne chaff alone. In contrast, maximal concentrations of IGF-1 were seen after 14 days of treatment. These changes were accompanied by increased plasma glucose, with no changes in triacylglycerols, non-esterified fatty acids and protein. Plasma urea was reduced by GRF, indicating that amino acids were diverted away from catabolism towards protein synthesis. These two sources of GRF were very similar in their endocrine and metabolic effects. This confirms similar observations regarding their effects on growth performance and carcass composition.

scholarly journals Effects of starvation on intermediary metabolism in the lactating cow. A comparison with metabolic changes occurring during bovine ketosis

1972 ◽  
Vol 128 (5) ◽  
pp. 1311-1318 ◽  
Author(s):  
G. D. Baird ◽  
R. J. Heitzman ◽  
K. G. Hibbitt
Keyword(s):  
Fatty Acids ◽  
Plasma Concentration ◽  
Dairy Cows ◽  
Blood Concentration ◽  
Ketone Bodies ◽  
Fat Metabolism ◽  
Metabolic Changes ◽  
Initial Increase ◽  
Blood Concentrations ◽  
Lactating Dairy Cows

1. The purpose of this study was to determine the nature of the metabolic changes associated with carbohydrate and fat metabolism that occurred in the blood and liver of lactating dairy cows during starvation for 6 days. 2. During starvation, the blood concentrations of the free fatty acids and ketone bodies increased, whereas that of citrate decreased. After an initial increase, the blood concentration of glucose subsequently declined as starvation progressed. Starvation caused a significant decrease in the plasma concentration of serine and a significant increase in that of leucine. 3. After 6 days of starvation the hepatic concentrations of oxaloacetate, citrate, phosphoenolpyruvate, 2-phosphoglycerate, 3-phosphoglycerate, glucose, glycogen, ATP and NAD+ had all decreased, as had the hepatic activities of phosphopyruvate carboxylase (EC 4.1.1.32) and pyruvate kinase (EC 2.7.1.40). 4. The above metabolic changes are similar to those previously found to occur in cows suffering from spontaneous ketosis (Baird et al., 1968; Baird & Heitzman, 1971). 5. Milk yield decreased progressively during starvation. 6. There were marked differences in the ability of individual animals to resist the onset of severe starvation ketosis.

The relationship between fatty liver and fertility in dairy cows: a farm investigation

1983 ◽  
Vol 101 (2) ◽  
pp. 499-502 ◽  
Author(s):  
I. M. Reid ◽  
Sally M. Dew ◽  
R. A. Collins ◽  
M. J. Ducker ◽  
Glenys A. Bloomfield ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Dairy Cows ◽  
Fatty Infiltration ◽  
Reproductive Physiology ◽  
Calving Interval ◽  
And Performance ◽  
The Relationship

Earlier studies have reported a relationship between degree of fatty infiltration of the liver after calving and calving interval (Reid, Roberts & Manston, 1979; Reid, 1983). The aimof the present investigation was to relate the severity of fatty liver with cow reproductive physiology and performance.

scholarly journals Blood Concentrations of Designer Benzodiazepines: Relation to Impairment and Findings in Forensic Cases

2020 ◽  
Vol 44 (8) ◽  
pp. 905-914 ◽  
Author(s):  
Gunhild Heide ◽  
Gudrun Høiseth ◽  
Gerrit Middelkoop ◽  
Åse Marit Leere Øiestad
Keyword(s):  
High Performance ◽  
Clinical Test ◽  
Concentration Data ◽  
Criminal Offenders ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
The Relationship ◽  
Designer Benzodiazepines

Abstract The use of designer benzodiazepines appears to be increasing in many countries, but data concerning blood concentrations are scarce, making interpretation of concentrations difficult. The aim of this study was to report blood concentrations of clonazolam, diclazepam, etizolam, flualprazolam, flubromazepam, flubromazolam and phenazepam and to investigate the relationship between blood concentrations and impairment. The concentration data are from blood samples collected from living cases (apprehended drivers and other drug offences) and medico-legal autopsies. The blood samples were analysed for the seven designer benzodiazepines mentioned above by ultra high performance liquid chromatography–tandem mass spectrometry. Positive cases from between 1 June 2016 and 30 September 2019 were included. Blood concentrations and the conclusion from a clinical test of impairment (when available) are reported. The presented seven benzodiazepines were detected in a total of 575 cases, where 554 of these cases concerned apprehended drivers or other criminal offenders. The number of findings and the median (range) concentrations were as follows: clonazolam, n = 22, 0.0041 mg/L (0.0017–0.053 mg/L); diclazepam, n = 334, 0.0096 mg/L (0.0016–0.25 mg/L); etizolam, n = 40, 0.054 mg/L (0.015–0.30 mg/L); flualprazolam, n = 10, 0.0080 mg/L (0.0033–0.056 mg/L); flubromazepam, n = 5, 0.037 mg/L (0.0070–0.70 mg/L); flubromazolam, n = 20, 0.0056 mg/L (0.0004–0.036 mg/L); and phenazepam, n = 138, 0.022 mg/L (0.0018–0.85 mg/L). A designer benzodiazepine was the only drug detected with relevance for impairment in 25 of the 554 living cases. The physician concluded with impairment in 19 of the 25 cases. Most of the concentrations in these cases were relatively similar to or higher than the median reported concentrations. The most frequent other drugs detected were amphetamine, tetrahydrocannabinol, clonazepam and methamphetamine. The presented blood concentrations can be helpful with the interpretation of cases involving one or more of these seven benzodiazepines. The results indicate that concentrations commonly observed in forensic cases are associated with impairment.

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