Effect of ACTH and oxytocin treatment on lactoferrin and citrate in cows' milk

1985 ◽  
Vol 52 (3) ◽  
pp. 379-389 ◽  
Author(s):  
Alex S. A. Thomas ◽  
Lloyd R. Fell
Keyword(s):  
Milk Yield ◽  
Antibacterial Properties ◽  
Significant Rise ◽  
Coliform Bacteria ◽  
Molar Ratio ◽  
Milk Secretion ◽  
Citrate Concentration ◽  
Lactating Cows ◽  
Cell Counts ◽  
Acth Treatment

SUMMARYSix dairy cows were treated before milkings with either oxytocin (Pitocin, 20 i.u.) or ACTH (Synacthen, 150 i.u.), principally to determine their effect on the ratio of citrate:lactoferrin concentrations in the milk. With ACTH treatment, after 3 d milk yield and citrate concentration decreased significantly, lactoferrin and bovine serum albumin (BSA) concentrations increased significantly. Somatic cell counts (SCC) increased temporarily in the milk of three of the cows which previously had > 100000 cells/ml. Lactoferrin yield remained fairly constant but citrate yield was significantly reduced. The citrate: lactoferrin molar ratio decreased from 1373 to 606. With oxytocin treatment, after 4 d milk yield first increased and then significantly decreased, citrate concentration decreased significantly while there were no significant changes in lactoferrin or BSA concentration or in the yield of any other milk constituents. The citrate: lactoferrin molar ratio decreased from 1621 to 1301. There were no significant changes in SCC either during treatment or 4 d after treatment but there was a significant rise at 16 d after treatment. It was concluded that in lactating cows both hormones affected citrate and lactoferrin concentrations in the direction that would improve the antibacterial properties of milk, but that this was accompanied by adverse effects on milk secretion. The extent of the change was not sufficient to be likely to produce inhibition of coliform bacteria.

The use of probiotics for the correction of disturbances of metabolism in cows

2021 ◽  
pp. 47-53
Author(s):  
O. V. Kovaleva ◽  
N. V. Sannikova ◽  
О. V. Shulepova ◽  
N. M. Kostomakhin ◽  
Yu. A. Karmatskikh
Keyword(s):  
Bacillus Subtilis ◽  
Milk Yield ◽  
Biochemical Parameters ◽  
Metabolic Disorders ◽  
Ketone Bodies ◽  
Animal Husbandry ◽  
Feed Additives ◽  
Coliform Bacteria ◽  
Lactating Cows ◽  
Microbial Number

Currently, there is a huge range of different feed additives on the market, which are widely used in animal husbandry, but they act simultaneously, i. e. by stopping their use the resulting effect is also lost. Metabolic disorders due to feeding errors begin imperceptibly without any precursors, and first of all this affects the biochemical parameters of the blood only later leads to alimentary diseases with deep often irreversible degenerative changes in organs and tissues. In practice, there are rarely disorders of any one type of metabolism more often there are combinations of various metabolic disorders, especially often this occurs in the absence of a complete balanced feeding. The purpose of the work was to evaluate the effectiveness of the use of a probiotic drug based on Bacillus subtilis in the watering of lactating cows for the correction of metabolic disorders. In order to assess the actual presence of probiotics in the water during the experiment its analysis has been carried out. It has shown that in the water that enters the distribution tank the total microbial number was 9×10[sup]5[/sup] CFU/ml, and in the drinker this indicator was at the level of 3,8×10[sup]5[/sup] CFU/ml. Common coliform bacteria and thermotolerant coliform bacteria have been not detected. Studies have shown that when using the drug based on Bacillus subtilis containing five strains of bacteria in an amount of 0,01 %, which is 6 ml/head/day in the watering of lactating cows during the increasing the milk yield period there was a tendency to correct metabolic disorders. There was the decrease in the content of ketone bodies by 11,9 %, the increase in glucose by 34 % and the decrease in urea by 7,1 mmol/l.

Effect of Bypass Protein on Milk Production and Economic of Lactating Crossbred Cows

2020 ◽  
Vol 7 (01) ◽  
Author(s):  
RATNESH K CHOUDHARY ◽  
A Saran ROY ◽  
N K SINGH3 ◽  
SANJAY KUMAR ◽  
RAUSHAN K SINGH
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
Feed Cost ◽  
Total Diet ◽  
T1 And T2 ◽  
Daily Milk Yield ◽  
On Farm ◽  
Daily Milk

An On-Farm Trial was conducted on 24 lactating crossbred cows for assessment of feeding formaldehyde treated mustard cake (bypass protein) on milk production and economic analysis of lactating cow. Cows were divided into three groups having 8 cows each, treatments were farmers’ practice (FP); (Control): The lactating animals under this group were fedas per the feeding schedule of the farmers (5 kg. dry roughage as rice straw + 6 hrs grazing as local grass and 4 kg. commercial concentrates), T1: The lactating animals under this group were fed as per farmers practicewith 12% mustard cake of total diet was provided to the cow by replacing the same amount of commercial concentrates andT2: The lactating animals under this group were fed as per farmers practicewith 12% formaldehyde treated mustard cake of total diet was provided to the cow by replacing same amount of commercial concentrates. The average daily milk yield of lactating cows under FP, T1 and T2 was 8.58, 8.82 and 9.85 kg per cow, respectively. Differences between FP and T2 were significant. The daily increase in milk yield was 1.27 kg and 1.03 kg in cows fed T2diet over the cows fed FP and T1diet, respectively. The B: C ratios for FP, T1 and T2 groups were 2.6, 3.0 and 3.3, respectively. The feed cost reduced in T2 group by Rs. 8.64 and increased milk production by 1.27 kg in respect to FP group.

Comparison of milk production, mastitis, lameness and days to conception of Brown Swiss, Holstein and Holstein cross Brown Swiss dairy cattle

2006 ◽  
Vol 2006 ◽  
pp. 86-86
Author(s):  
G Wellwood ◽  
J K Margerison
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Milk Production ◽  
Milk Yield ◽  
Complex Disease ◽  
Economic Loss ◽  
Brown Swiss ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Holstein Friesian

Mastitis is a complex disease causing inflammation of the udder, which has been estimated to cost the dairy farmer between £40-£117/cow per year (Stott et al., 2002). Economic loss occurs as a result of discarded milk, reduced milk yield and milk quality, increased vet costs and an increase in replacement costs. The objective of this study was to examine the effect of breed on the incidence of mastitis and somatic cell counts and milk production capabilities of Holstein Friesian, Brown Swiss and Brown Swiss crossbred cows.

scholarly journals Characterization of Short-Term Heat Stress in Holstein Dairy Cows Using Altered Indicators of Metabolomics, Blood Parameters, Milk MicroRNA-216 and Characteristics

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 722
Author(s):  
Jang-Hoon Jo ◽  
Jalil Ghassemi Nejad ◽  
Dong-Qiao Peng ◽  
Hye-Ran Kim ◽  
Sang-Ho Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Blood Parameters ◽  
Negative Effects ◽  
Short Term ◽  
Lactating Cows ◽  
Humidity Index ◽  
Holstein Dairy Cows

This study aims to characterize the influence of short-term heat stress (HS; 4 day) in early lactating Holstein dairy cows, in terms of triggering blood metabolomics and parameters, milk yield and composition, and milk microRNA expression. Eight cows (milk yield = 30 ± 1.5 kg/day, parity = 1.09 ± 0.05) were homogeneously housed in environmentally controlled chambers, assigned into two groups with respect to the temperature humidity index (THI) at two distinct levels: approximately ~71 (low-temperature, low-humidity; LTLH) and ~86 (high-temperature, high-humidity; HTHH). Average feed intake (FI) dropped about 10 kg in the HTHH group, compared with the LTLH group (p = 0.001), whereas water intake was only numerically higher (p = 0.183) in the HTHH group than in the LTLH group. Physiological parameters, including rectal temperature (p = 0.001) and heart rate (p = 0.038), were significantly higher in the HTHH group than in the LTLH group. Plasma cortisol and haptoglobin were higher (p < 0.05) in the HTHH group, compared to the LTLH group. Milk yield, milk fat yield, 3.5% fat-corrected milk (FCM), and energy-corrected milk (ECM) were lower (p < 0.05) in the HTHH group than in the LTLH group. Higher relative expression of milk miRNA-216 was observed in the HTHH group (p < 0.05). Valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, lactic acid, 3-phenylpropionic acid, 1,5-anhydro-D-sorbitol, myo-inositol, and urea were decreased (p < 0.05). These results suggest that early lactating cows are more vulnerable to short-term (4 day) high THI levels—that is, HTHH conditions—compared with LTLH, considering the enormous negative effects observed in measured blood metabolomics and parameters, milk yield and compositions, and milk miRNA-216 expression.

scholarly journals The Effects of Turnip (Brassica rapa) Extract on the Growth Performance and Health of Broilers

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 867
Author(s):  
Reza Eghbaldost-Jadid ◽  
Mehran Nosrati ◽  
Behrouz Rasouli ◽  
Alireza Seidavi ◽  
Clive J. C. Phillips
Keyword(s):  
Broiler Chickens ◽  
Growth Promotion ◽  
Antibacterial Properties ◽  
Low Density Lipoproteins ◽  
Feed Additives ◽  
Coliform Bacteria ◽  
Broiler Chicks ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Four Levels

There are concerns about inclusion of antibiotics in the feed of broiler chickens, because of the development of antibiotic resistance, leading to a search for alternative feed additives. Turnip extract is known to have antibacterial properties but has not been tested in the diet of broiler chickens. We allocated 200 broiler chicks to receive one of four levels of turnip extract in their water, 0, 150, 300 or 450 ppm, or a standard antibiotic, Virginiamycin, over a 42-day growing period. Although initially there were detrimental effects of providing 450 ppm, overall the 150 ppm level of supplementation increased weight gain, compared with birds given Virginiamycin, and decreased gizzard weight. Birds given 150 ppm or Virginiamycin had increased low-density lipoproteins (LDLs) and reduced very low-density lipoproteins (VLDLs) in their blood serum and reduced antibody responses to sheep red blood cells, compared to birds in the 450 ppm treatment. Birds given turnip extract at 450 ppm had fewer Gram-negative lactose and coliform bacteria than those provided with no turnip extract, and those provided with 150–300 ppm had the same as those provided with Virginiamycin. Turnip extract could potentially replace antibiotics included in the feed of broiler chickens for growth promotion and the control of bacterial infection of the gastrointestinal tract.

A COMPARISON OF TWO METHODS OF COMBINING ALFALFA CUBES WITH CORN SILAGE FED TO LACTATING COWS

1977 ◽  
Vol 57 (3) ◽  
pp. 559-565
Author(s):  
L. J. FISHER
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Corn Silage ◽  
Feeding Time ◽  
Plasma Urea ◽  
Lactating Cows ◽  
Fat Yield ◽  
Changeover Period ◽  
Feeding Systems

Sixteen Holstein cows in early lactation were assigned to a switch-back experiment consisting of two experimental periods 35 days in length separated by a 7-day changeover period. Treatments consisted of two feeding systems, alfalfa cubes ensiled with corn silage or alfalfa cubes fed with the corn silage. When cows were fed corn–alfalfa cube silage they consumed more forage, 2.26 vs. 2.16% of body weight per cow per day than when they were fed the alfalfa cubes with the silage. Fat-corrected milk yield was somewhat higher, 25.4 vs. 24.0 kg/day; fat percent and fat yield were greater, 3.13 vs. 2.70% and 918 vs. 814 g/day for corn–alfalfa cube silage as compared to cubes added at feeding time. However, milk yield was greater, 29.9 vs. 29.1 kg/day, and milk lactose percent was greater, 5.26 vs. 5.18% for cows fed cubes at feeding time compared to cows fed corn–alfalfa cube silage. There was no difference between feeding systems in blood minerals or metabolites with the exception of plasma urea nitrogen which was higher, 20.2 vs. 17.2 mg %, for the alfalfa cubes fed with the corn silage. Apparent dry matter and protein digestibility for the total ration were 68.1 and 71.6% when corn alfalfa cube silage was fed compared to 65.4 and 68.5% when the cubes were fed with the corn silage (P < 0.05). It was concluded from this study that alfalfa cubes ensiled with corn silage was an effective method of increasing the dry matter and protein content of corn silage and in improving the digestibility of the total ration.

SUBSTITUTION OF RAPESEED MEAL FOR SOYBEAN MEAL AS A SOURCE OF PROTEIN FOR LACTATING COWS

1976 ◽  
Vol 56 (2) ◽  
pp. 233-242 ◽  
Author(s):  
L. J. FISHER ◽  
DOROTHY S. WALSH
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Rapeseed Meal ◽  
Milk Composition ◽  
Hormone Metabolism ◽  
Thyroid Hormone Metabolism ◽  
Lactating Cows ◽  
Lower Blood ◽  
Concentrate Mixture ◽  
Low Erucic Acid

Sixteen lactating Holstein cows were randomly assigned to treatment sequences in a 4 × 4 changeover experiment. Experimental periods were 28 days in length separated by 7 days for changeover. Cows were fed corn silage free choice and gradually increasing amounts of one of four dairy concentrates containing 0, 11, 22, or 34% rapeseed meal (RSM) of a Canadian, experimental, low glucosinolate–low erucic acid variety. Concentrate intake (kg dry matter/day), milk yield (kg/day) and body weight change (kg/28 days) were 8.45a, 8.71a, 8.37a and 7.17b; 24.40a, 24.03ab, 22.92bc and 21.96c and 7.6b, 22.4b, 13.0ab, and 3.0b for 0, 11, 22 and 34% RSM mixtures, respectively. There was no influence of treatment on milk composition. Ration dry matter (DM) and nitrogen digestibilities declined (P < 0.05) as the RSM in the concentrate mixture increased. The amount of nitrogen excreted in the urine was greatest for cows fed 0% RSM and least for cows fed 34% RSM. Cows fed concentrate mixtures containing 34% RSM had a lower blood thyroxine level than others (P < 0.05), but RSM-containing concentrates had no apparent effect on the conversion of thyroxine to triiodothyronine. Results of the trial indicated that this experimental variety of RSM had no detrimental effect on concentrate intake, DM digestibility, milk composition or thyroid hormone metabolism when included at levels up to 22% in the concentrate mixture. However, milk yield and apparent digestibility of ration nitrogen were decreased (P < 0.05) by the 22% RSM mixture. It was not certain from the current findings whether the depression in digestibility was due largely to the high oil content of the meal or due to the higher fiber content of the RSM-supplemented rations.

scholarly journals 127 Predicting Dry Matter Intake of Gestating and Lactating Beef Cows

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 58-58
Author(s):  
Megan A Gross ◽  
Claire Andresen ◽  
Amanda Holder ◽  
Alexi Moehlenpah ◽  
Carla Goad ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Milk Yield ◽  
Feed Intake ◽  
Beef Cows ◽  
Multiple Regression Equation ◽  
Validation Data ◽  
Data Set ◽  
Lactating Cows ◽  
Downward Bias ◽  
Protein Supply

Abstract In 1996, the NASEM beef cattle committee developed and published an equation to estimate cow feed intake using results from studies conducted or published between 1979 and 1993 (Nutrient Requirements of Beef Cattle). The same equation was recommended for use in the most recent version of this publication (2016). The equation is sensitive to cow weight, diet digestibility and milk yield. Our objective was to validate the accuracy of this equation using more recent published and unpublished data. Criteria for inclusion in the validation data set included projects conducted or published within the last ten years, direct measurement of forage intake, adequate protein supply, and pen feeding (no tie stall or metabolism crate data). The validation data set included 29 treatment means for gestating cows and 26 treatment means for lactating cows. Means for the gestating cow data set was 11.4 ± 1.9 kg DMI, 599 ± 77 kg BW, 1.24 ± 0.14 Mcal/kg NEm per kg of feed and lactating cow data set was 14.5 ± 2.0 kg DMI, 532 ± 116.3 kg BW, and 1.26 ± 0.24 Mcal NEm per kg feed, respectively. Non intercept models were used to determine equation accuracy in predicting validation data set DMI. The slope for linear bias in the NASEM gestation equation did not differ from 1 (P = 0.07) with a 3.5% positive bias. However, when the NASEM equation was used to predict DMI in lactating cows, the slope for linear bias significantly differed from 1 (P &lt; 0.001) with a downward bias of 13.7%. Therefore, a new multiple regression equation was developed from the validation data set: DMI= (-4.336 + (0.086427 (BW^.75) + 0.3 (Milk yield)+6.005785(NEm)), (R-squared=0.84). The NASEM equation for gestating beef cows was reasonably accurate while the lactation equation underestimated feed intake.

THE MAINTENANCE OF LACTATION IN THE RABBIT AFTER HYPOPHYSECTOMY

1969 ◽  
Vol 43 (4) ◽  
pp. 651-662 ◽  
Author(s):  
A. T. COWIE ◽  
P. E. HARTMANN ◽  
A. TURVEY
Keyword(s):  
Growth Hormone ◽  
Immune Responses ◽  
Milk Yield ◽  
Human Growth Hormone ◽  
Synergistic Effects ◽  
Human Growth ◽  
Milk Composition ◽  
Bovine Growth Hormone ◽  
Partial Recovery ◽  
Milk Secretion

SUMMARY Twenty-one rabbits were hypophysectomized during the second week of lactation. Hypophysectomy inhibited milk secretion within 3–7 days. Restoration of the milk yield to pre-operative levels was obtained when daily injections of either sheep prolactin (50 or 100 i.u.) or human growth hormone (HGH) (2·5 or 5 mg.) were administered; partial recovery of the milk yield was obtained with 25 i.u. sheep prolactin/day or 1·25 mg. HGH/day. Neither bovine growth hormone (BGH), adrenocorticotrophin (ACTH) nor cortisol acetate had any significant restorative effects on milk secretion or synergistic effects when combined with sheep prolactin or HGH. The probable development of immune responses to sheep prolactin and HGH was observed. After hypophysectomy, changes in milk composition were found which were similar in trend to the more gradual normal changes observed in late lactation. Sheep prolactin and HGH restored the composition of the milk as well as the yields to normal levels, and both hormones caused rapid increases in lactose yields. The pigeon crop-stimulating potency of the HGH preparation was low (equivalent to 3·7 i.u. sheep prolactin/mg.), yet its lactogenic and galactopoietic potencies were high thus emphasizing the unreliability of assessing the mammotrophic potencies of HGH preparations in birds.

scholarly journals On the Nature of the Cellular Elements Present in Milk. Part III. The Milk of Animals other than the Cow

1911 ◽  
Vol 11 (1) ◽  
pp. 97-104 ◽  
Author(s):  
R. Tanner Hewlett ◽  
Sidney Villar ◽  
Cecil Revis
Keyword(s):  
General Consideration ◽  
Milk Secretion ◽  
High Cell ◽  
Cell Counts ◽  
Artificial Condition ◽  
Definite Manner ◽  
Blood Elements ◽  
Great Stress ◽  
Very High ◽  
Stimulation Of

The general consideration of these results only tends to confirm the conclusions already arrived at. A uniform type of life evidently tends to a fairly uniform excretion of tissue cells from the udder. The case of Goat II also emphasises the effect of outside causes in increasing temporarily this excretion, while some of the samples of human milk show plainly that very high cell counts are not by any means necessarily connected with any diseased or disordered condition of the mammary gland.We again emphasise the view we have already stated, viz. that in the cow the udder must be looked upon as an organ which has by breeding and selection been brought to an artificial condition of milk secretion and that this has been accompanied by a stimulation of the tissues to cell proliferation and that this proliferation may quite easily be caused to become abnormally great, leading to the appearance of an increased number of cells in the secretion. In support of this view we lay great stress on the fact constantly noticed by us, viz. that when the cell count is high for any reason, the cells themselves are always well-defined, showing little signs of degeneration, and also stain in a much more characteristic and definite manner, a fact which is diffcult to explain if they are to be considered to be blood elements.

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