Influence of milk somatic cell content on Parmigiano-Reggiano cheese yield

2015 ◽  
Vol 82 (2) ◽  
pp. 222-227 ◽  
Author(s):  
Andrea Summer ◽  
Piero Franceschi ◽  
Paolo Formaggioni ◽  
Massimo Malacarne
Keyword(s):  
Somatic Cell ◽  
Milk Fat ◽  
Crude Protein ◽  
Whey Proteins ◽  
Titratable Acidity ◽  
Cell Content ◽  
Significant Drop ◽  
True Protein ◽  
Morning Milk ◽  
Parmigiano Reggiano

The aim of this study was to determine the influence of the somatic cell content (SCC) of milk on Parmigiano-Reggiano cheese yield, produced in commercial cheese factories under field conditions. The study was carried out following the production of 56 batches of Parmigiano-Reggiano in 13 commercial cheese factories by processing milk collected from Italian Friesian cattle herds. The vat-milk (V-milk) used for making each cheese batch was obtained by mixing evening milk (partially skimmed following spontaneous separation of fat overnight, natural creaming) and morning milk. The batches of cheese produced were divided into 5 classes according to the SCC value of the evening milk determined prior to natural creaming (class 1, from 0 to 200 000; 2, 201 000–300 000; 3, 301 000–400 000; 4, 401 000–500 000; 5, over 501 000 cells/ml). The cheese yield was calculated as the amount of 24-h cheese, expressed in kilograms, obtained from 100 kg of V-milk (24 h ACY). The values of fat, crude protein, true protein, casein and 24 h ACY of V-milk were negatively correlated with the somatic cell score (SCS) of the evening milk. Conversely, a positive correlation was observed between chloride and SCS. Fat, protein fractions (crude protein, casein and whey proteins), P and titratable acidity of V-milk were positively correlated with its 24 h ACY, while chloride, pH and SCS showed a negative correlation. A significant drop in 24 h ACY was observed in classes 3, 4 and 5, therefore when the SCC of the evening milk exceeded 300 000 cells/ml. Finally a lower recovery of milk fat in cheese was observed as SCC of evening milk increase.

scholarly journals Influence of composite κ-casein and β-lactoglobulin genotypes on composition, rennetability and heat stability of milk of cows of Slovak Pied breed

2008 ◽  
Vol 52 (No. 9) ◽  
pp. 292-298 ◽  
Author(s):  
A. Michalcová ◽  
Z. Krupová
Keyword(s):  
Protein Content ◽  
Milk Fat ◽  
Least Squares Method ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Heat Stability ◽  
Lactation Stage ◽  
Negative Effect ◽  
Starch Gel ◽  
True Protein

The effect of the phenotypic combination of genetic variants κ-CN/β-Lg on the contents of technologically and nutritionally important components of milk as well as technological properties of milk was investigated in this paper. The samples of milk were collected from 72 cows of Slovak Pied breed in the second lactation coming from three farms, once from the different lactation stage. The genotyping of milk samples was determined by the horizontal electrophoretic separation of milk proteins on starch gel with addition of urea and mercaptoethanol. Statistical analysis was carried out in the programmes Statistica 6.0 and Statgraphic Plus 5.1 by the least-squares method using the GLM. A non-significant association of <i>B</i> allele in the κ-CN/β-Lg phenotypic combination with milk fat, crude protein content and true protein content was found out. A positive significant effect of κ-CN/β-Lg <i>BB/BB</i> on casein content and casein number was confirmed. The value of casein number increased in the following order: <i>BB/BB</i> > <i>AB/AB</i> > <i>AA/AA<i> (78.43%:77.53%:75.78%). With regard to whey proteins and heat stability, a negative effect of B allele in the phenotypic combination κ-CN/β-Lg was found out.

Dairy maturation of milk used in the manufacture of Parmigiano-Reggiano cheese: effects on physico-chemical characteristics, rennet-coagulation aptitude and rheological properties

2008 ◽  
Vol 75 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Massimo Malacarne ◽  
Andrea Summer ◽  
Paolo Formaggioni ◽  
Piero Franceschi ◽  
Sandro Sandri ◽  
...  
Keyword(s):  
Whey Proteins ◽  
Titratable Acidity ◽  
Operating Conditions ◽  
Chemical Characteristics ◽  
Proteose Peptone ◽  
Rennet Coagulation ◽  
Physico Chemical ◽  
Microbial Development ◽  
The Difference ◽  
Parmigiano Reggiano

The aim of this research was to study the effects of dairy maturation on the physico-chemical characteristics and technological properties of milk used for Parmigiano-Reggiano cheese manufacture. Three different operating conditions (CF1, CF2 and CF3) were considered. Full cream milk from the evening milking was stored on the farm and delivered to the cheese factory in churns (CF1) or in thermoregulated tanks at a temperature not lower than 18°C (CF2 and CF3). The natural creaming (10–12 h overnight) was performed in a traditional large flat vat containing 10–12 hl (CF1 and CF2) or in thermoregulated large flat vats containing 60 hl at about 15°C (CF3). Twenty-four, 24 and 22 maturation trials were performed in CF1, CF2, and CF3, respectively, during 2 consecutive years. A significant increase (P⩽0·05) in pH during the maturation of milk was observed in CF1 and CF2. The increase of pH was higher (P⩽0·05) in CF1 than CF2 and CF3. The values of titratable acidity were higher (P⩽0·05) in partially skimmed evening (PS) milk than in full cream (FC) milk in each operative condition. The increase observed in CF2 was higher than those reported in CF1 and CF3. Compared with FC milk, PS milk showed lower values (P⩽0·05) of casein and casein number and higher contents (P⩽0·05) of whey proteins and, particularly, proteose-peptone. The increase of proteose-peptone – per 100 g SNF or 100 g casein – was significantly higher (P⩽0·05) in CF1 than in CF2 and, in particular, than in CF3. A higher increase (P⩽0·05) of resistance to compression was observed in CF1 with respect to CF3. CF2 variation was not different with respect to either CF1 or CF3. Variation of the difference between PS and FC milks (PS-FC) in pH, TBC and fat were clearly lower in CF3 than CF1. This means that the control of milk temperature throughout the whole maturation phase offers a greater control of both microbial development and extent of creaming.

scholarly journals Effect of dietary crude protein and forage contents on enteric methane emissions and nitrogen excretion from dairy cows simultaneously

2016 ◽  
Vol 56 (3) ◽  
pp. 312 ◽  
Author(s):  
M. Niu ◽  
J. A. D. R. N. Appuhamy ◽  
A. B. Leytem ◽  
R. S. Dungan ◽  
E. Kebreab
Keyword(s):  
Protein Synthesis ◽  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Crude Protein ◽  
Microbial Protein ◽  
Ch4 Emissions ◽  
Lactating Dairy Cows ◽  
Enteric Methane ◽  
True Protein

The study aimed to examine, simultaneously, the effects of changing dietary forage and crude protein (CP) contents on enteric methane (CH4) emissions and nitrogen (N) excretion from lactating dairy cows. Twelve post-peak lactating Holstein cows (157 ± 31 days postpartum; mean ± s.d.) were randomly assigned to four treatments from a 2 × 2 factorial arrangement of two dietary forage levels [37.4% (LF) vs 53.3% (HF) of DM] and two dietary CP levels [15.2% (LP) vs 18.5% (HP) of DM] in a 4 × 4 Latin square design with four 18-day periods. Alfalfa hay was the sole source of dietary forage. Cows were fed ad libitum and milked twice daily. During the first 14 days, cows were housed in a free-stall barn, where enteric CH4 emissions were measured using the GreenFeed system from Days 8 to 14 in each period. Cows were then moved to metabolic cages, where faeces and urine output (kg/cow.day) were measured by total collection from Days 16 to 18 of each period. No dietary forage by CP interactions were detected for DM intake, milk production, enteric CH4 emissions, or N excretions. There was a tendency for DM intake to increase 0.6 kg/day in cows fed LF (P = 0.06). Milk production increased 2.1 kg/day in LF compared with HF (P < 0.01). Milk fat content decreased in cows fed LF compared with HF (1.07 vs 1.17 kg/day; P < 0.01). Milk contents of true protein, lactose and solid non-fat were greater in cows fed LF (P < 0.01). No difference in DM intake, milk yield and milk contents of true protein, lactose and solid non-fat was found between cows fed HP or LP. However, milk fat content increased 0.16 kg/day in cows fed HP (P < 0.05). Enteric CH4 emissions, and CH4 per unit of DM intake, energy-corrected milk, total digested organic matter and neutral detergent fibre were not affected by dietary CP, but decreased by LF compared with HF (P < 0.01). Milk true protein N was not affected by dietary CP content but was higher for LF compared with HF. Dietary N partitioned to milk true protein was greater in cows fed LF compared with HF (29.4% vs 26.7%; P < 0.01), also greater in cows fed LP compared with HP (30.8% vs 25.2%; P < 0.01). Dietary N partitioned to urinary N excretion was greater in cows fed HP compared with LP (39.5% vs 29.6%; P < 0.01) but was not affected by dietary CP content. Dietary N partitioned to faeces was not affected by dietary CP but increased in cows fed LP compared with HP (34.2% vs 27.8%; P < 0.01). Total N excretion (urinary plus faecal) as proportion to N intake did not differ between HP and LP, but tended to be lower in cows fed LF compared with the HF diet (64.2% vs 67.9%; P = 0.09). Both milk urea N (P < 0.01) and blood urea N (P < 0.01) declined with decreasing dietary CP or forage contents. Based on purine derivative analysis, there was a tendency for interaction between dietary CP and forage content on microbial protein synthesis (P < 0.09). Rumen microbial protein synthesis tended to be lower for high forage and low protein treatments. Increasing dietary forage contents resulted in greater CH4 emission (g/kg of energy-corrected milk) and manure N excretion (g/kg of energy-corrected milk) intensities of lactating dairy cows. Cows receiving reduced CP diets had low manure N outputs and improved milk true protein production efficiencies, regardless of dietary forage content.

Effects of the full cream milk somatic cell content on the characteristics of vat milk in the manufacture of Parmigiano-Reggiano cheese

2009 ◽  
Vol 33 (S1) ◽  
pp. 281-283 ◽  
Author(s):  
P. Franceschi ◽  
A. Summer ◽  
S. Sandri ◽  
P. Formaggioni ◽  
M. Malacarne ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Content ◽  
Parmigiano Reggiano

scholarly journals Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 456
Author(s):  
Hewa Bahithige Pavithra Chathurangi Ariyarathne ◽  
Martin Correa-Luna ◽  
Hugh Thomas Blair ◽  
Dorian John Garrick ◽  
Nicolas Lopez-Villalobos
Keyword(s):  
Gene Ontology ◽  
New Zealand ◽  
Dairy Cows ◽  
Candidate Genes ◽  
Milk Fat ◽  
Crude Protein ◽  
Single Locus ◽  
Protein Utilization ◽  
Grazing Dairy Cows ◽  
Genomic Regions

The objective of this study was to identify genomic regions associated with milk fat percentage (FP), crude protein percentage (CPP), urea concentration (MU) and efficiency of crude protein utilization (ECPU: ratio between crude protein yield in milk and dietary crude protein intake) using grazing, mixed-breed, dairy cows in New Zealand. Phenotypes from 634 Holstein Friesian, Jersey or crossbred cows were obtained from two herds at Massey University. A subset of 490 of these cows was genotyped using Bovine Illumina 50K SNP-chips. Two genome-wise association approaches were used, a single-locus model fitted to data from 490 cows and a single-step Bayes C model fitted to data from all 634 cows. The single-locus analysis was performed with the Efficient Mixed-Model Association eXpedited model as implemented in the SVS package. Single nucleotide polymorphisms (SNPs) with genome-wide association p-values ≤ 1.11 × 10−6 were considered as putative quantitative trait loci (QTL). The Bayes C analysis was performed with the JWAS package and 1-Mb genomic windows containing SNPs that explained > 0.37% of the genetic variance were considered as putative QTL. Candidate genes within 100 kb from the identified SNPs in single-locus GWAS or the 1-Mb windows were identified using gene ontology, as implemented in the Ensembl Genome Browser. The genes detected in association with FP (MGST1, DGAT1, CEBPD, SLC52A2, GPAT4, and ACOX3) and CPP (DGAT1, CSN1S1, GOSR2, HERC6, and IGF1R) were identified as candidates. Gene ontology revealed six novel candidate genes (GMDS, E2F7, SIAH1, SLC24A4, LGMN, and ASS1) significantly associated with MU whose functions were in protein catabolism, urea cycle, ion transportation and N excretion. One novel candidate gene was identified in association with ECPU (MAP3K1) that is involved in post-transcriptional modification of proteins. The findings should be validated using a larger population of New Zealand grazing dairy cows.

scholarly journals Hyperketonemia Predictions Provide an On-Farm Management Tool with Epidemiological Insights

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1291
Author(s):  
Ryan S. Pralle ◽  
Joel D. Amdall ◽  
Robert H. Fourdraine ◽  
Garrett R. Oetzel ◽  
Heather M. White
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Milk Yield ◽  
Milk Fat ◽  
Somatic Cell Count ◽  
Past Research ◽  
Management Tool ◽  
Milk Analysis ◽  
Linear Regression Models ◽  
High Throughput Detection

Prediction of hyperketonemia (HYK), a postpartum metabolic disorder in dairy cows, through use of cow and milk data has allowed for high-throughput detection and monitoring during monthly milk sampling. The objective of this study was to determine associations between predicted HYK (pHYK) and production parameters in a dataset generated from routine milk analysis samples. Data from 240,714 lactations across 335 farms were analyzed with multiple linear regression models to determine HYK status. Data on HYK or disease treatment was not solicited. Consistent with past research, pHYK cows had greater previous lactation dry period length, somatic cell count, and dystocia. Cows identified as pHYK had lower milk yield and protein percent but greater milk fat, specifically greater mixed and preformed fatty acids (FA), and greater somatic cell count (SCC). Differential somatic cell count was greater in second and fourth parity pHYK cows. Culling (60d), days open, and number of artificial inseminations were greater in pHYK cows. Hyperketonemia prevalence decreased linearly in herds with greater rolling herd average milk yield. This research confirms previously identified risk factors and negative outcomes associated with pHYK and highlights novel associations with differential SCC, mixed FA, and preformed FA across farm sizes and production levels.

Herring silage as a protein supplement for young cattle

1991 ◽  
Vol 71 (4) ◽  
pp. 1187-1196 ◽  
Author(s):  
J. W. G. Nicholson ◽  
D. A. Johnson
Keyword(s):  
Amino Acids ◽  
Free Amino ◽  
Crude Protein ◽  
Fish Meal ◽  
Unsaturated Fatty Acids ◽  
Rumen Fluid ◽  
Potassium Sorbate ◽  
Protein Supplement ◽  
Young Cattle ◽  
True Protein

Fish silage made by grinding herring and adding formic acid, β-hydroxytoluene and potassium sorbate was evaluated as a protein supplement for young cattle. Only about 15% of the crude protein in the herring silage was true protein. Ammonia N accounted for 8% of the crude protein and most of the rest was peptides and free amino acids. The crude protein of herring silage was as resistant as fish meal to deamination when fermented in rumen fluid, and more resistant than soybean or casein. The herring silage was readily accepted by Holstein heifers fed hay or grass–legume silage with potatoes (7 kg d−1) and a supplement (1.5 kg d−1). Feed intake and weight gain were similar when the heifers were fed hay with either soybean meal or herring silage but were higher when forage silage replaced the hay. Rumen fluid NH3-N and blood urea levels were normal, even for cattle fed the high non-protein N diet of forage silage with herring silage. The herring silage depressed rumen fluid acetate levels and increased propionate in the heifers fed hay + potatoes, probably because of the unsaturated fatty acids in the herring. Well-made herring silage was a suitable protein supplement for young cattle fed forage and potato diets. Key words: Herring silage, fish silage, potatoes, cattle, protein degradation

Aggregation of whey proteins during storage of acidified milk

1976 ◽  
Vol 43 (1) ◽  
pp. 45-51 ◽  
Author(s):  
P. J. Argyle ◽  
N. Jones ◽  
R. C. Chandan ◽  
J. F. Gordon
Keyword(s):  
Physical Properties ◽  
Milk Fat ◽  
Dairy Products ◽  
Storage Temperature ◽  
Whey Proteins ◽  
Disc Electrophoresis ◽  
Protein Fractions ◽  
Irreversible Aggregation ◽  
Acid Whey

SummarySlow, irreversible aggregation of whey proteins in acidified milk or whey at pH 3·4–4·6 held for up to 10 d at 35–45°C was revealed by the reduction of discrete bands in disc electrophoresis. The aggregation was confirmed by precipitation of protein observed in stored, acid whey. The rate of aggregation of all protein fractions increased with the acidity and the storage temperature. It was enhanced by the presence of casein, but was unaffected by the presence of milk fat or by pasteurization of the fresh, unacidified milk at 70°C. The effect may contribute to the physical properties of certain fresh cheeses and other cultured dairy products.

Chemical changes in bovine milk fat globule membrane caused by heat treatment and homogenization of whole milk

2002 ◽  
Vol 69 (4) ◽  
pp. 555-567 ◽  
Author(s):  
SUNG JE LEE ◽  
JOHN W. SHERBON
Keyword(s):  
Heat Treatment ◽  
Membrane Proteins ◽  
Milk Fat ◽  
Whey Proteins ◽  
Bovine Milk ◽  
Chemical Changes ◽  
Milk Fat Globule Membrane ◽  
Whole Milk ◽  
Milk Fat Globule ◽  
Fat Globule

The effects of heat treatment and homogenization of whole milk on chemical changes in the milk fat globule membrane (MFGM) were investigated. Heating at 80 °C for 3–18 min caused an incorporation of whey proteins, especially β-lactoglobulin (β-lg), into MFGM, thus increasing the protein content of the membrane and decreasing the lipid. SDS-PAGE showed that membrane glycoproteins, such as PAS-6 and PAS-7, had disappeared or were weakly stained in the gel due to heating of the milk. Heating also decreased free sulphydryl (SH) groups in the MFGM and increased disulphide (SS) groups, suggesting that incorporation of β-lg might be due to association with membrane proteins via disulphide bonds. In contrast, homogenization caused an adsorption of caseins to the MFGM but no binding of whey proteins to the MFGM without heating. Binding of caseins and whey proteins and loss of membrane proteins were not significantly different between milk samples that were homogenized before and after heating. Viscosity of whole milk was increased when milk was treated with both homogenization and heating.

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