scholarly journals Focusing on fatty acid profile in milk from different species after in vitro digestion

2018 ◽  
Vol 85 (2) ◽  
pp. 257-262 ◽  
Author(s):  
Antonella Santillo ◽  
Lucia Figliola ◽  
Maria G Ciliberti ◽  
Mariangela Caroprese ◽  
Rosaria Marino ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Human Milk ◽  
Fatty Acid Profile ◽  
In Vitro Digestion ◽  
Milk Formula ◽  
Formula Milk ◽  
Caprine Milk ◽  
Chain Fatty Acids

We report the fatty acid profile of raw milk and of the corresponding digested milk from different sources (human milk, formula milk and donkey, bovine, ovine and caprine milk) to gain information on the nutritional quality of different milk sources in infant nutrition.Short chain fatty acids (SC-FA) were higher in bovine and caprine milk, intermediate in ovine and donkey and lower in human and formula milk. Medium chain fatty acids (MC-FA) showed the highest values for bovine and caprine milk and the lowest for donkey and formula milk, whereas long chain fatty acids (LC-FA) were the highest in donkey and formula milk and intermediate in human milk.The percentage distribution of fatty acids liberated after in vitro digestion did not reflect the patterns found in the corresponding milk sources. In particular, MC free fatty acids (MC-FFA) showed the highest and the lowest values in donkey and in formula milk, LC-FFA showed the highest value in human milk. The total FFA was highest in human milk, lowest in formula milk and intermediate in donkey, bovine, ovine, and caprine milk.

scholarly journals An Analysis of Oxidative Changes and the Fatty Acid Profile in Stored Poultry Sausages with Liquid and Microencapsulated Fish Oil Additives

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4293
Author(s):  
Krzysztof Kawecki ◽  
Jerzy Stangierski ◽  
Piotr Konieczny
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Profile ◽  
In Vitro Digestion ◽  
Negative Influence ◽  
Omega 3 ◽  
Epa And Dha ◽  
Microencapsulated Fish Oil

This study deals with the fatty acid profile and oxidative changes (TBARS) in vacuum-packed (VP) or modified-atmosphere-packed (MAP) finely-comminuted poultry sausages with liquid fish oil and microencapsulated fish oil (MC) additives. An analysis of omega-3 fatty acids (EPA and DHA) showed that their content in the samples with the fish oil additive decreased from the initial value of 0.22 g∙100 g−1 of the product to 0.18 g∙100 g−1 (MAP) and 0.17 g∙100 g−1 (VP), respectively. After in vitro digestion, the total EPA and DHA content in the sample with microencapsulated oil amounted to 0.17 g∙100 g−1 of the product. The TBARS values showed the VP samples with both forms of the fish oil additive had the lowest values on the first day of storage. Storage of the samples for 21 days caused a slight increase in the degree of lipid oxidation. The research indicated that the forms of the oil additive did not have a negative influence on the sensory features or the physicochemical properties of the sausages. The EPA and DHA levels in samples with liquid fish oil and those with oil microcapsules were sufficient for the sausage producer to declare high content of these fatty acids in accordance with the current EC regulation.

scholarly journals Effect of storage on fatty acid profile of butter from cows fed whole or ground flaxseed with or without monensin

2010 ◽  
Vol 39 (10) ◽  
pp. 2297-2303 ◽  
Author(s):  
Daniele Cristina da Silva-Kazama ◽  
Geraldo Tadeu dos Santos ◽  
Paula Toshimi Matumoto Pintro ◽  
Jesuí Vergílio Visentainer ◽  
Ricardo Kazama ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profile ◽  
Saturated Fatty Acids ◽  
Relative Percentage ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Ground Flaxseed ◽  
Chain Fatty Acids

Eight Holstein cows with body weight 570 ± 43 kg and 60 ± 20 lactation days were distributed in a double Latin square design with four 21-day periods to determine the effects of feeding ground or whole flaxseed with or without monensin supplementation (0.02% on a dry matter basis) on fatty acid profile of butter stored for 15 and 45 days. Ground flaxseed supply, in comparison to whole flaxseed, reduced relative percentages of 16:0, cis7-16:1, 17:0, and cis10-17:1 but it increased those of cis9,trans11-18:2, cis3-18:3, and omega 3 fatty acids in butter fat, reducing relative percentage of medium-chain fatty acids and increasing the content of polyunsaturated fatty acids. Supplementation with monensin increased relative percentages of cis9,trans11-18:2 and tended to increase relative percentage of 17:0 and decrease that of saturated fatty acids in butter. Butter from cows fed diet with monensin presented lower relative percentages of cis 6-20:4. Relative percentages of cis 9-16:1, cis10-17:1, 18:0, trans11-18:1, cis9-18:1, cis3-18:3, cis6-20:4 in butter stored for 15 days were higher than those stored for 45 days and the relative percentages of cis3-20:5 tended to decrease with the increase of storage period. As a result, relative percentages of saturated fatty acids and medium-chain fatty acids increased with storage time, while those of monounsaturated and long-chain fatty acids decreased. Butter enriched with polyunsaturated fatty acids may have a shorter shelf life due to the negative effect of storage on fatty acid profile which may cause oxidation and rancidity.

Short-chain fatty acid (SCFA) and medium-chain fatty acid (MCFA) concentrations in human milk consumed by infants born at different gestational ages and the variations in concentration during lactation stages

2020 ◽  
Vol 11 (2) ◽  
pp. 1869-1880 ◽  
Author(s):  
Xinyue Dai ◽  
Tinglan Yuan ◽  
Xinghe Zhang ◽  
Qin Zhou ◽  
Huiya Bi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Human Milk ◽  
Short Chain Fatty Acids ◽  
Term Infant ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Medium Chain ◽  
Naturally Occurring ◽  
Chain Fatty Acids

Short-chain fatty acids and medium-chain fatty acids (4:0, 6:0 and 8:0) are naturally occurring in human milk triacylglycerol and are present in highest amounts in mature full-term infant milk (1.47 ± 0.66 mg g−1 fat).

scholarly journals Short-Chain and Total Fatty Acid Profile of Faeces or Plasma as Predictors of Food-Responsive Enteropathy in Dogs: A Preliminary Study

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 89
Author(s):  
Cristina Higueras ◽  
Ana I. Rey ◽  
Rosa Escudero ◽  
David Díaz-Regañón ◽  
Fernando Rodríguez-Franco ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Propionic Acid ◽  
Fatty Acid Profile ◽  
Total Fatty Acid ◽  
Isovaleric Acid ◽  
Short Chain ◽  
Control Group ◽  
Inflammatory Status ◽  
Chain Fatty Acids

The aim of this study was to evaluate differences in short-chain fatty acids (SCFAs) and the total fatty acid profile of faeces or plasma as possible indicators of FRE in comparison with healthy dogs. FRE dogs had a lower concentration (p = 0.026) of plasma α-tocopherol as an indicator of the oxidative status of the animal, and lower C20:5n-3 (p = 0.033), C22:5n-3 (p = 0.005), polyunsaturated fatty acids (PUFA) (p = 0.021) and n-6 (p = 0.041) when compared with the control dogs; furthermore, sick dogs had higher proportions of plasma C20:3n-6 (p = 0.0056). The dogs with FRE showed a decrease in the production of faecal levels of SCFAs, mainly propionic acid (C3) (p = 0.0001) and isovaleric acid (iC5) (p = 0.014). FRE dogs also had a lower proportion of C15:0 (p = 0.0003), C16:1n-9 (p = 0.0095), C16:1n-7 (p = 0.0001), C20:5n-3 (p = 0.0034) and monounsaturated fatty acids (p = 0.0315), and tended to have lower n-3 (p = 0.058) and a reduced desaturase activity index in the stool when compared with the control group. However, the dogs with chronic enteropathy tended to have greater C20:4n-6 (p = 0.065) in their faeces as signs of damage at the intestinal level. The faecal parameters were better predictors than plasma. The highest correlations between faecal odd-chain, medium- or long-chain fatty acids and SCFAs were observed for C15:0 that correlated positively with faecal acetic acid (C2) (r = 0.72, p = 0.004), propionic acid (r = 0.95, p = 0.0001), isobutyric acid (iC4) (r = 0.59, p = 0.027) and isovaleric acid (r = 0.64, p = 0.0136), as well as with total SCFAs (r = 0.61, p = 0.02). Conversely, faecal C20:4n-6 showed a high inverse correlation (r = −0.83, p = 0.0002) with C2 and C3 (r = −0.59, p = 0.027). Canine inflammatory bowel disease (IBD) activity (CIBDAI) index correlated negatively mainly with faecal measurements, such as C3 (r = −0.869, p = 0.0005) and C15:0 (r = −0.825, p = 0.0018), followed by C16:1/C16:0 (r = −0.66, p= 0.0374) and iC5 (r = −0.648, p = 0.0310), which would indicate that these fatty acids could be good non-invasive indicators of the chronic inflammatory status, specifically FRE.

scholarly journals In Vitro and In Vivo Digestibility of Soybean, Fish, and Microalgal Oils, and Their Influences on Fatty Acid Distribution in Tissue Lipid of Mice

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5357
Author(s):  
Bo-Ram Na ◽  
Jeung-Hee Lee
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Distribution ◽  
Docosapentaenoic Acid ◽  
In Vitro Digestion ◽  
Fish Oils ◽  
Microalgal Oil ◽  
Mouse Tissues

The digestion rates of microalgal (docosahexaenoic acid, DHA, 56.8%; palmitic acid, 22.4%), fish (DHA, 10.8%; eicosapentaenoic acid, EPA, 16.2%), and soybean oils (oleic, 21.7%; linoleic acid, 54.6%) were compared by coupling the in vitro multi-step and in vivo apparent digestion models using mice. The in vitro digestion rate estimated based on the released free fatty acids content was remarkably higher in soybean and fish oils than in microalgal oil in 30 min; however, microalgal and fish oils had similar digestion rates at longer digestion. The in vivo digestibility of microalgal oil (91.49%) was lower than those of soybean (96.50%) and fish oils (96.99%). Among the constituent fatty acids of the diet oils, docosapentaenoic acid (DPA) exhibited the highest digestibility, followed by EPA, DHA, palmitoleic, oleic, palmitic, and stearic acid, demonstrating increased digestibility with reduced chain length and increased unsaturation degree of fatty acid. The diet oils affected the deposition of fatty acids in mouse tissues, and DHA concentrations were high in epididymal fat, liver, and brain of mice fed microalgal oil. In the present study, microalgal oil showed lower in vitro and in vivo digestibility, despite adequate DHA incorporation into major mouse organs, such as the brain and liver.

Composition of milk cephalins

1968 ◽  
Vol 46 (3) ◽  
pp. 205-210 ◽  
Author(s):  
G. M. Moore ◽  
J. B. M. Rattray ◽  
D. M. Irvine
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Human Milk ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Phosphatidyl Serine ◽  
Variable Component ◽  
Layer Chromatography ◽  
Palmitic Acids

Lipid was extracted from lyophilized samples of cow, goat, sheep, horse, and human milk. The cephalins were isolated by thin-layer chromatography and ascertained to be the major, but variable component of all milk phospholipids examined except in the case of the horse. The predominant cephalin type was found to be phosphatidyl ethanolamine (PE), while phosphatidyl serine (PS) was determined to be either absent or to account for approximately 20% of the total cephalins. Lysocephalins were generally absent. The fatty acid composition of PE and PS was characterized by high levels of oleic and palmitic acids and by the virtual lack of long-chain polyunsaturated fatty acids. Individual differences in fatty acid content were slight among members of the same species, but human milk PE showed considerable variations in composition. PE was determined to be more highly unsaturated than PS with the unsaturated fatty acids occurring primarily at the β-position of the PE molecule. The results obtained on the chemical structure of milk cephalins have been discussed in relationship to the suggested activity of these compounds on the blood coagulation process in vitro.

scholarly journals Regiospecific Positioning of Palmitic Acid in Triacylglycerol Structure of Enzymatically Modified Lipids Affects Physicochemical and In Vitro Digestion Properties

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4015
Author(s):  
Hyeon-Jun Chang ◽  
Jeung-Hee Lee
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
In Vitro Digestion ◽  
Lower Content ◽  
Melting Points ◽  
Fat Crystals ◽  
Triacylglycerol Structure ◽  
Acetone Fractionation

Tripalmitin-(PPP, 81.2%), 1,3-dipalmitoyl-2-oleoylglycerol-(POP, 64.4%), 1,2-dipalmitoyl-3-oleoylglycerol-(PPO, 86.5%), and 1,3-dioleoyl-2-palmitoylglycerol-(OPO, 50.2%)-rich lipids with different regiospecific positions of palmitic acid (P) were synthesized via acetone fractionation and lipase-catalyzed acidolysis, and their physicochemical and hydrolytic characteristics were compared. Triacylglycerols (TAGs) with higher content of P, wherein P was at the sn-1 (or 3) position, had higher melting points, crystallization temperatures, and packing densities of fat crystals compared to those with a lower content of P, and with P at the sn-2 position. The in vitro digestion degree calculated as released fatty acid (FA) (%) at 30, 60, and 120 min was in the following order: OPO-rich > PPO-rich > POP-rich lipids. At 120 min, in vitro digestion of the OPO-rich lipid released 92.6% of fatty acids, resulting in the highest digestibility, while 89.7% and 87.2% of fatty acids were released from the OPO-rich and PPO-rich lipids, respectively. Over the digestion period, the TAG and monoacylglycerol (MAG) contents decreased, while the diacylglycerol (DAG) content initially increased and then decreased, and the 1,2-DAG content exceeded the 1,3-DAG content. Therefore, the content and stereospecific position of P attached to a specific TAG affected the physicochemical and in vitro digestion characteristics of the lipids.

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