Comparison of immunomodulatory properties of purified lactoferrin, lactoperoxidase and β-casein in sheep

1998 ◽  
Vol 65 (4) ◽  
pp. 697-701 ◽  
Author(s):  
CHUN W. WONG ◽  
DENNIS L. WATSON ◽  
GEOFFREY O. REGESTER ◽  
GEOFFREY W. SMITHERS
Keyword(s):  
Biomedical Applications ◽  
Bovine Milk ◽  
Milk Proteins ◽  
Biologically Active ◽  
Immunological Effects ◽  
Bovine Milk Proteins ◽  
Proteins And Peptides

Bovine milk contains a variety of proteins and peptides that are biologically active (Ogra & Ogra, 1978; Duncan & McArthur, 1981; Newby et al. 1982; Juto, 1985; Stoeck et al. 1989; Mincheva-Nilsson et al. 1990; Watson, 1990; Barta et al. 1991; Politis et al. 1991; Fiat et al. 1993). Our laboratory has a long-term interest in some purified milk proteins, particularly lactoferrin (LF), lactoperoxidase (LP) and β-casein (β-CN), which have been shown to be immunologically significant. Some of our recent studies on these bovine milk proteins, particularly β-CN, indicated that their in vitro immunological effects did not always parallel their in vivo activities (Wong et al. 1996a, b; 1997a, b). This study was designed to investigate and compare the capacity of these purified bovine milk proteins to modulate a range of components that are vital to in vivo immune responses in sheep, with a view to providing further information on their potential in biomedical applications. To achieve this objective, a sensitive lymphatic cannulation model was employed that allows in vivo immune components and their functions to be measured in lymph collected under physiological conditions.

Biologically active casein peptides implicated in immunomodulation

1989 ◽  
Vol 56 (3) ◽  
pp. 357-362 ◽  
Author(s):  
D. Migliore-Samour ◽  
F. Floc'h ◽  
P. Jollès
Keyword(s):  
Synthetic Peptides ◽  
Viral Infections ◽  
Milk Proteins ◽  
Biologically Active ◽  
Passive Immunity ◽  
Maternal Milk ◽  
Enzymic Digestion ◽  
Immunomodulating Peptides

SummaryMaternal milk should not only be considered as a nutrient, but also as a protecting agent against aggressions from the neonate's new environment. Breastfeeding facilitates transmission of a passive immunity by multifunctional factors which have a direct effect on the neonate's resistance to bacterial and viral infections. Among these factors are the main milk proteins, the caseins: during enzymic digestion of human and bovine caseins, immunomodulating peptides are released. Corresponding synthetic peptides stimulated in vitro phagocytic activity of murine and of human macrophages and exerted in vivo a protective effect against Klebsiella pneumoniae infection of mice. These data suggest that casein peptides may exert a stimulating function on the immune system of the newborn.

Transport across Caco-2 monolayers of peptides arising from in vitro digestion of bovine milk proteins

2013 ◽  
Vol 139 (1-4) ◽  
pp. 203-212 ◽  
Author(s):  
Gianluca Picariello ◽  
Giuseppe Iacomino ◽  
Gianfranco Mamone ◽  
Pasquale Ferranti ◽  
Olga Fierro ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Milk Proteins ◽  
In Vitro Digestion ◽  
Bovine Milk Proteins

scholarly journals Delivery of Nucleic Acids and Nanomaterials by Cell-Penetrating Peptides: Opportunities and Challenges

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Yue-Wern Huang ◽  
Han-Jung Lee ◽  
Larry M. Tolliver ◽  
Robert S. Aronstam
Keyword(s):  
Nucleic Acids ◽  
Biomedical Applications ◽  
Cell Penetrating Peptides ◽  
Biologically Active ◽  
The Past ◽  
Cell Penetrating ◽  
Cellular Entry

Many viral and nonviral systems have been developed to aid delivery of biologically active molecules into cells. Among these, cell-penetrating peptides (CPPs) have received increasing attention in the past two decades for biomedical applications. In this review, we focus on opportunities and challenges associated with CPP delivery of nucleic acids and nanomaterials. We first describe the nature of versatile CPPs and their interactions with various types of cargoes. We then discussin vivoandin vitrodelivery of nucleic acids and nanomaterials by CPPs. Studies on the mechanisms of cellular entry and limitations in the methods used are detailed.

Effect of prior dietary exposure to cows' milk protein on antigen-specific and nonspecific cellular proliferation in mice

2005 ◽  
Vol 72 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Susanne Brix ◽  
Orit H Magyar ◽  
Vibeke Barkholt ◽  
Hanne Frøkiær
Keyword(s):  
Immune Cells ◽  
Cellular Proliferation ◽  
Ex Vivo ◽  
Bovine Milk ◽  
Milk Proteins ◽  
Dietary Exposure ◽  
Dietary Components ◽  
The Impact ◽  
Proteins And Peptides

The impact of dietary components on the immune system is gaining increased attention in the effort to develop safe food products, some even with health-promoting potential, as well as to improve the basic understanding of the immunomodulatory potential of common food components. In such studies, which are mainly based on experiments in vitro, it is important to be able to differentiate nonspecific activation of immune cells induced by dietary components from ex vivo restimulation of antigen-specific cells that might be present in cell cultures owing to prior dietary exposure to the antigens in cell donors. Focusing on the immunostimulatory potential of cows' milk proteins and peptides, we studied the impact of prior dietary exposure to cows' milk on proliferation of murine immune cells upon ex vivo stimulation with bovine milk proteins. Nonspecific proliferation induced by β-casein peptides was further assessed on cells from mice bred on a cows'-milk-free diet. Regarding the dietary effect, we found that prior oral intake of cows' milk proteins affected cell proliferation induced by culturing with cows' milk proteins in vitro, as spleen cells from mice fed a milk-containing diet showed a significantly greater proliferative response than did cells from mice bred on a cows'-milk-free diet. Studies of immune enhancing potentials of β-casein peptides showed that some peptides stimulate proliferation of immune cells nonspecifically. In conclusion, these findings stress the importance of employing immune cells from mice unexposed to cows' milk for studies of the immunomodulating capacity of cows' milk proteins and peptides, in order to rule out the interference caused by antigen-specific immune responses. By using such cells, we here show that some β-casein peptides possess the potential to induce proliferation in immune cells in a nonspecific manner.

scholarly journals In vitro immunogenicity of various native and thermally processed bovine milk proteins and their mixtures

2018 ◽  
Vol 101 (10) ◽  
pp. 8726-8736 ◽  
Author(s):  
Dimuthu Bogahawaththa ◽  
Rabia Ashraf ◽  
Jayani Chandrapala ◽  
Osaana Donkor ◽  
Todor Vasiljevic
Keyword(s):  
Bovine Milk ◽  
Milk Proteins ◽  
Thermally Processed ◽  
Bovine Milk Proteins

Characterization and kinetics of gastric emptying of peptides derived from milk proteins in the preruminant calf

1992 ◽  
Vol 59 (4) ◽  
pp. 437-447 ◽  
Author(s):  
P. Scanff ◽  
M. Yvon ◽  
S. Thirouin ◽  
J.-P. Pelissffir
Keyword(s):  
Gastric Emptying ◽  
Skim Milk ◽  
Milk Proteins ◽  
Reversed Phase ◽  
Pancreatic Enzymes ◽  
Biologically Active ◽  
Empty Stomach ◽  
Kinetics Of

SummaryThe gastric emptying kinetics of peptides derived from milk protein were studied in vivo in preruminant calves by collecting and characterizing the whole effluent leaving the stomach for 12 h after ingestion of crude skim milk. Peptides were isolated by reversed-phase HPLC and identified. Particular attention was paid to biologically active peptides and to peptides that could be precursors of biologically active sequences. A gastrin inhibitor, the caseinomacropeptide, was emptied from the stomach only during the first 0·5 h of digestion and rapidly hydrolysed. Precursors of immunostimulatory peptides from αs1 - and β-caseins were emptied throughout digestion in the gastric effluent. A precursor of β-casomorphins (peptide 58–93 of β-casein) was emptied from the stomach 3·5 h after the meal when it was taken on an empty stomach. From this precursor, peptides that may be resistant to hydrolysis by intestinal peptidase were obtained after in vitro hydrolysis by pancreatic enzymes. A phosphopeptide (fragment 110–142 of αs1-casein) was also found in digesta after a few hours of digestion. When the meal was not taken on an empty stomach, these peptides were emptied in the first digesta at a low concentration. The potential activity of these peptides is discussed. The results support the hypothesis that active sequences could still be present in the gut after the action of pancreatic enzymes.

In vitro digestibility and immunoreactivity of bovine milk proteins

2016 ◽  
Vol 190 ◽  
pp. 581-587 ◽  
Author(s):  
Andrew B. Do ◽  
Kristina Williams ◽  
Ondulla T. Toomer
Keyword(s):  
Bovine Milk ◽  
Milk Proteins ◽  
In Vitro Digestibility ◽  
Bovine Milk Proteins

scholarly journals Mineral-binding milk proteins and peptides; occurrence, biochemical and technological characteristics

2000 ◽  
Vol 84 (S1) ◽  
pp. 91-98 ◽  
Author(s):  
Gerd E. Vegarud ◽  
T. Langsrud ◽  
C. Svenning
Keyword(s):  
Trace Elements ◽  
Specific Binding ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Inorganic Ions ◽  
Specific Binding Sites ◽  
Long Time ◽  
Proteins And Peptides

Minerals and trace elements in cow's milk occur as inorganic ions and salts or form complexes with proteins and peptides, carbohydrates, fats and small molecules. The main mineral binder or chelators of calcium are the caseins, αs1-casein, αs2-casein, β-casein and κ-casein, but also whey proteins and lactoferrin bind specific minerals like calcium, magnesium, zinc, iron, sodium and potassium. Less documented is the binding of trace elements. Peptides obtained byin vitroorin vivohydrolysis act as mineral trappers through specific and non-specific binding sites. They may then function as carriers, chelators, of various minerals and thus enhance or inhibit bioavailability. Peptides from milk proteins have found interesting new applications in the food industry as products with improved functionality or as ingredients of dietary products, or used in pharmaceutical industry. Fortification of foods with minerals in a low concentration has for a long time been used in some countries to overcome mineral deficiency, which is an increasing problem in humans. These types of foods are being used to create a new generation of super foods in the industry today.

scholarly journals STUDY OF THE EFFECT OF BIOLOGICALLY ACTIVE SUBSTANCES ON THE REPRO-DUCTION OF THE TRANSPLANTED CELL CULTURE LINE

2021 ◽  
Vol 246 (2) ◽  
pp. 157-160
Author(s):  
E.M. Plotnikova ◽  
◽  
I.A. Nesterova ◽  
Z.G. Churina ◽  
A.S. Saifullin ◽  
...  
Keyword(s):  
Cell Lines ◽  
Animal Cell ◽  
Biologically Active ◽  
High Biological Activity ◽  
Long Term Storage ◽  
Cell Culture Line ◽  
Chitin And Chitosan

The present study was carried out with take into account the high biological activity of bi-opolymers, in particular chitin and chitosan for animal cell cultures in vivo and in vitro. The aim of the study was investigating the effect of apiphytoextract on the growth and reproduction of trans-planted Taurus-1 cell lines after their recriopreservation for long-term storage in the liquid nitrogen. The use of apiphytoextract as part of the culture medium in the cultivation of hydrated cell lines allowed the use of the method of cell recriopreservation for reproduction of viruses, which are used for vaccine production.

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