Proliferating cell nuclear antigen in laryngeal cancer

AbstractThe expression of proliferating cell nuclear antigen in paraffin sections from 154 cases of laryngeal squamous cell carcinoma were examined. There was significant difference in PCNA expression between the control group and cancer patients (p<0.001). The mean score of PCNA was higher in patients with poor prognosis than in patients with satisfactory outcome after treatment (p<0.05). There was no significant correlation between the PCNA count and the patient's age and sex, T and N stage and site of the tumour.Univariate analysis revealed that the PCNA score correlated with the patients' survival rates. In multivariate analysis the prognostic value of PCNA was on the statistical borderline (p = 0.049). In our study clinical features like N and T status had a more important influence on survival rate. Nevertheless it appears that the immunohistological examination of PCNA in paraffin section could be a complementary prognostic tool for laryngeal carcinoma. PCNA expression may also be a valuable tool for differentiating malignant from benign laryngeal epithelium.

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Effect of the aqueous extract of Sida cordifolia on liver regeneration after partial hepatectomy

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502006000700009 ◽

2006 ◽

Vol 21 (suppl 1) ◽

pp. 37-39 ◽

Cited By ~ 13

Author(s):

Renata Lemos Silva ◽

Gustavo Barreto de Melo ◽

Valdinaldo Aragão de Melo ◽

Ângelo Roberto Antoniolli ◽

Paulo Roberto Teixeira Michellone ◽

...

Keyword(s):

Oral Administration ◽

Liver Regeneration ◽

Partial Hepatectomy ◽

Aqueous Extract ◽

Proliferating Cell Nuclear Antigen ◽

Nuclear Antigen ◽

Hepatic Regeneration ◽

Control Group ◽

Sida Cordifolia ◽

Proliferating Cell

PURPOSE: The use of medicinal plants for the treatment of human diseases has increased worldwide. Many of them are used by oral administration and, after absorption, may affect many organs. Therefore, this study aimed at assessing the effects of the aqueous extract of Sida cordifolia leaves, popularly known in Brazil as "malva-branca", on liver regeneration. METHODS: Twenty rats were divided into four groups: control, Sida100, Sida200 and Sida400 groups. All animals were submitted to oral administration of distilled water, 100, 200 and 400 mg/kg of the aqueous extract of Sida cordifolia, respectively. Immediately after this, they underwent 67% partial hepatectomy. Twenty four hours later, their livers were removed. Hepatic regeneration was assessed by immunohistochemical staining for proliferating cell nuclear antigen (PCNA) using the PC-10 monoclonal antibody. RESULTS: Sida100 and Sida200 groups disclosed higher liver regeneration indices than control group (p<0.001 and p<0.05, respectively). CONCLUSION: The aqueous extract of Sida cordifolia stimulates liver regeneration after 67% partial hepatectomy in rats.

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Effect of Clostridium on proliferating cell nuclear antigen and ghrelin in the small intestine of fattening pigs fed with deoxynivalenol

World Mycotoxin Journal ◽

10.3920/wmj2020.2569 ◽

2020 ◽

pp. 1-14

Author(s):

F. Li ◽

L. Huang ◽

H. Chen ◽

X. Yuan ◽

C. Wang ◽

...

Keyword(s):

Small Intestine ◽

Proliferating Cell Nuclear Antigen ◽

Animal Health ◽

Blood Parameters ◽

Intestinal Morphology ◽

Toxic Effects ◽

Nuclear Antigen ◽

Control Group ◽

Proliferating Cell ◽

Fattening Pigs

Grains and feed are severely contaminated by deoxynivalenol (DON) globally, threatening both human and animal health. Research on bio-degradation of DON, in general, is gaining attention. The aim of this research was to estimate the effect of Clostridium sp. WJ06 as a microbiological detoxification of DON based on the expression and distribution of proliferating cell nuclear antigen (PCNA) as well as ghrelin in the small intestine. A total of 24 fattening pigs were randomly divided into three groups. The control group was fed with a basic diet, the DON group was fed with DON at 5.0 mg/kg in feed, and the DON+C group was provided DON feed with Clostridium sp. WJ06. Several selected blood parameters, the intestinal morphology, and the expression and distribution of PCNA and ghrelin, were evaluated. The results proved that the selected blood parameters were altered, the intestinal villi were damaged, the epithelium was shed, as well as the expression and distribution of PCNA and ghrelin were changed by DON exposure. These toxic effects were prevented by the addition of Clostridium sp. WJ06. In short, the addition of Clostridium sp. WJ06 to the feed may eliminate the toxic effects of DON in fattening pigs. An underlying mechanism is likely modulation of the expression and distribution of PCNA and ghrelin.

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The immunohistochemical expression of p21WAF1/Cip1 and Proliferating cell nuclear antigen in laryngeal squamous cell carcinomas

The Journal of Laryngology & Otology ◽

10.1017/s0022215106002726 ◽

2006 ◽

Vol 120 (12) ◽

pp. 1042-1048 ◽

Cited By ~ 4

Author(s):

X Li ◽

S Izumaru ◽

K Sakamoto ◽

Y Miyajima ◽

T Nakashima

Keyword(s):

Squamous Cell ◽

Proliferating Cell Nuclear Antigen ◽

Inverse Correlation ◽

Squamous Cell Carcinomas ◽

Nuclear Antigen ◽

Clinicopathological Parameters ◽

T Stage ◽

N Stage ◽

Tissue Specimens ◽

Proliferating Cell

The purpose of this study was to assess the significance of the expression of p21WAF1/Cip1 and proliferating cell nuclear antigen (PCNA) in human laryngeal squamous cell carcinomas. Forty-eight patients (25 supraglottic, 23 glottic) who had undergone operations between 1998 and 2003 were included in this study. The Envision immunohistochemistry method was utilized to stain these tissue specimens. The results showed that the immunostaining of p21WAF1/Cip1 do not show any correlation with differentiation, N stage, metastasis, survival, recurrence or the laryngeal site of involvement. A significant inverse correlation was detected between p21WAF1/Cip1 and the T stage. In contrast, the percentage of PCNA labelled cells showed a significant correlation with the T stage, but not with other clinicopathological parameters. There were differences in the expression of p21WAF1/Cip1 and PCNA between the supraglottic and the glottic carcinomas. In conclusion, our findings suggest that p21WAF1/Cip1 and PCNA may play an important role in the progression of carcinoma of the supraglottic larynx.

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Retinoic Acid Reduces Glomerular Injury in a Rat Model of Glomerular Damage

Journal of the American Society of Nephrology ◽

10.1681/asn.v1181479 ◽

2000 ◽

Vol 11 (8) ◽

pp. 1479-1487 ◽

Cited By ~ 4

Author(s):

JÜRGEN WAGNER ◽

CLAUDIUS DECHOW ◽

CHRISTIAN MORATH ◽

INGO LEHRKE ◽

KERSTIN AMANN ◽

...

Keyword(s):

Retinoic Acid ◽

Rat Model ◽

Proliferating Cell Nuclear Antigen ◽

Treated Group ◽

Nuclear Antigen ◽

Control Group ◽

Glomerular Injury ◽

Proliferating Cell ◽

Vehicle Treated Group ◽

Glomerular Damage

Abstract.In the reaction of kidneys to injury, cytokine-driven proliferation plays an important role and precedes the development of glomerulosclerosis. There is great interest in agents that may interfere with such proliferation. Therefore, a rat model of mesangioproliferative glomerulonephritis (induced by anti-Thy1.1) was studied, and the effects of all-trans-retinoic acid (all-trans-RA) and isotretinoin, powerful antiproliferative and anti-inflammatory substances, on glomerular damage and cell proliferation were examined. Vehicle-injected control rats were compared with rats treated with daily subcutaneous injections of 10 mg/kg body wt all-trans-RA or 40 mg/kg body wt isotretinoin (n= 9 to 11 per group), using either a pretreatment (days -2 through 8) or posttreatment (days +3 through +8) protocol,i.e., starting before or after the induction of anti-Thy1.1 nephritis, respectively. All-trans-RA prevented the BP increase evoked by anti-Thy1.1 (anti-Thy1.1/vehicle, 112.2 ± 4.8 mmHg; anti-Thy1.1/RA, 87.5 ± 2.5 mmHg;P< 0.001). Treatment with all-trans-RA or isotretinoin produced a 70% decrease in the urinary albumin excretion rate (P< 0.02). Periodic acid-Schiff staining of saline-perfused kidneys (day 8) revealed significantly fewer glomerular cells in RA-treated nephritic rats (anti-Thy1.1/vehicle, 97 ± 3.1 cells/glomerulus; anti-Thy1.1/RA, 80 ± 4.4;P< 0.02; control/vehicle, 69 ± 1.2). No difference was observed between all-trans-RA and isotretinoin treatment. The capillary occlusion scores were significantly lower for the anti-Thy1.1/RA-treated group (1.9 ± 0.1) than for the anti-Thy1.1/vehicle-treated group (2.9 ± 0.5,P< 0.001). In the anti-Thy1.1/vehicle-treated group, 11.9 ± 1.1 glomerular cells were proliferating cell nuclear antigen-positive; however, in the anti-Thy1.1/RA-treated group, only 5.3 ± 0.8 cells were proliferating cell nuclear antigen-positive (P< 0.002; control, 2.2 ± 0.2). Glomerular mitoses were reduced by 67% in the anti-Thy1.1/RA-treated group, compared with the anti-Thy1.1/control group (P< 0.002). Glomerular staining for platelet-derived growth factor B-chain was significantly reduced in anti-Thy1.1-treated nephritic rats in the presence of isotretinoin or all-trans-RA, compared with the vehicle-treated group (P< 0.001). It is concluded that all-trans-RA limits glomerular proliferation, glomerular lesions, and albuminuria in an established model of renal damage. The findings point to retinoids as potential novel modulators of glomerular injury.

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