Pathogenicity of the coccidium Eimeria crandallis in laboratory lambs

Parasitology ◽  
1985 ◽  
Vol 91 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Janet Catchpole ◽  
M. W. Gregory

Ten colostrum-deprived, coccidia-free lambs were reared artificially in indoor cages. At 4 weeks of age they were allocated to 5 groups of 2: Groups 1–3 received 104 105 and 106 sporulated oocysts of Eimeria erandallis/lamb respectively, each as a single inoculum. Group 4 lambs received daily doses increasing over a 4-week period. Groups 1–3 showed diarrhoea and weight loss from about day 14, followed by slow recovery from about day 30. The diarrhoea was accompanied by a wave of oocyst output and a reduction in serum albumin and plasma alkaline phosphatase activity. One lamb in group 4 died of an unrelated condition on day 15; the other showed no clinical effect of the oocyst inoculations and its oocyst output was related only to the first week or two of inoculation. Lambs in Group I showed no clinical effect or oocyst output following challenge with 106 oocysts on day 28. E. crandallis showed high pathogenicity and immunogenicity. Its pathogenicity was not closely related to infecting dose within the range 104–106.

2021 ◽  
Vol 40 (2) ◽  
pp. 36-40
Author(s):  
Carmen Beatriz Borges Fortes ◽  
Susana Maria Werner Samuel

The purpose of this study was to evaluate the shown efectiveness by different ways of storage of amalgam residues, to reduce the liberation of mercury from such residues to the air ambience and to compare with lhe highest value established by the NR-15 (regulation norm number 15) from the decroe number 3214, of the brazilian labor ministry, dated from june, 8 h of 1978. As mercury source, amalgam in capsules of the brand Dispersalloy (Dentsplay) was used after grinding, in the mechanic amalgamizer varimix II (caulk), the quantity of amalgam in cach capsule was collected from a sieve that had a mesh with mm of width to standardize lhe fragmentation. The fragmented amalgam was stored in jars made of transparent polypropylene, that could store 5()() ml; one of the jars was kept in a dry atmosphere (group l), and the others were immersed in distilled water (group 2), in glycerin (group 3) and in a solution of dentistry x-ray fixer (group 4). These jars were kept tightly shut, and put inside a bigger polypropylene recipient, during the time they were stored. Thirty minutes after the storage of the fragments, the jars were opened and air samples were collected around each of the four jars. The same procedure was performed 30 days after the initial storage. A Pump Sampler of Air manufactured by SKCâ was used to do the air collection. The air samples were analyzed by a spectrophotorneter of atomic absorption, to determinate the quantity of mercury. This analysis showed the following mercury concentration rates in the air samples, from the first and the second collection of the four groups, in the following order: group I 6,100 mg/m3 and 0,816 mg/m3 ; group 2 - 0,252 mg/rn3 and 0,157 mg/m 3 ; group 3 — 0,071 mg/m3 and 0,005 mg/m3 ; groui) 4 — 0,256 mg/m3 and 0,005 mg/m3. The results showed that glycerin is the safest way to store amalgam residues, since the quantity of mercuor vapor found in the air, in both collections: the first one and the other 30 days after, never surpassed the highest value established by NR-15 (Regulation Norm number 15), also demonstrating, bigger security, since the initial period of storage of these residues.


1977 ◽  
Vol 23 (11) ◽  
pp. 2148-2150 ◽  
Author(s):  
R H Eaton

Abstract Two methods for measuring plasma alkaline phosphatase activity are compared: one makes use of phenyl phosphate, carbonate-bicarbonate buffer, and continuous-flow methodology; the other of p-nitrophenyl phosphate, diethanolamine buffer, and reaction-rate analysis. Results by the methods correlate well (r = 0.98) over a wide range of values (up to 10-fold the upper limit of normal). A factor can therefore be applied to convert results by one method into those that would be obtained by the other. The possibility that the presence of different proportions of isoenzymes in the plasma will affect this factor is considered. We have used the new method, with a conversion factor, as the routine method of alkaline phosphatase measurement in a clinical chemistry laboratory, with no problems.


1993 ◽  
Vol 21 (6) ◽  
pp. 811-813 ◽  
Author(s):  
L. Kashyap ◽  
L. N. Yaddanapudi ◽  
Sandhya

The incidence and degree of hypoxaemia during induction of balanced anaesthesia and endotracheal intubation were studied prospectively in 80 healthy adults undergoing elective surgery randomly divided into four equal groups of 20. Group I was preoxygenated for three minutes. The other three groups were not preoxygenated. Groups 1 and 2 were ventilated with 100% oxygen, while Groups 3 and 4 were ventilated with 50% and 33% oxygen respectively. Anaesthesia was induced with thiopentone 3-5 mg/kg and endotracheal intubation was done after ventilating for one minute with the chosen gas. Arterial desaturation was measured by pulse oximetry. In Groups 1-3 there was a significant increase and in Group 4 a significant decrease in saturation from the preinduction value. The arterial oxygen saturation was similar in Groups 1 and 2. Two patients in Group 3 and four in Group 4 had hypoxaemia. This incidence was not statistically significant. We conclude that ventilation with 100% oxygen for one minute prior to intubation and preoxygenation for three minutes are equally effective in preventing hypoxaemia during induction.


2018 ◽  
Vol 69 (8) ◽  
pp. 2081-2088 ◽  
Author(s):  
Alin Alexandru Odor ◽  
Edwin Sever Bechir ◽  
Deborah Violant ◽  
Victoria Badea

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.


2017 ◽  
Vol 05 (02) ◽  
pp. 084-089
Author(s):  
Alisha Dhingra ◽  
Ashu Gupta ◽  
Anshu Minocha ◽  
Nayantara Sen

Abstract Aim: The aim of this study was to compare the reversal of shear bond strength of composite to bleached enamel immediately after bleaching followed by application of various antioxidant solutions. Material and Methods: Seventy central incisors were divided into seven groups. Groups I and II served as unbleached and bleached controls respectively. Groups III, IV, V, VI and VII served as the experimental groups and were subjected to 37.5% hydrogen peroxide bleaching followed by 10 min application of 10% sodium ascorbate, 25% alpha-tocopherol, 6.5% grape seed extract, 5% lycopene and 5% green tea extract respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA followed by Post Hoc Multiple Comparisons test. Results: The bond strength values for Group I (positive control) were maximum and significantly different than all the other groups except Grape seed extract group (Group V). When compared to Group II (bleached control), all the groups showed significantly higher bond strength. Significant difference in the bond strength values were seen between Group III (10% sodium ascorbate) and Group V. Also values for Group V were significantly different from Group VI (5% lycopene). All the other values showed insignificantly different results. Conclusion: All the antioxidant solutions improved the shear bond strength values after bleaching but only Grape seed extract application reversed the values to the non bleached levels. Lycopene was least effective. Other antioxidants showed comparable results.


2014 ◽  
Vol 881-883 ◽  
pp. 757-760
Author(s):  
Xiao Qing Ren ◽  
Li Zhen Ma ◽  
Xin Yi He

The objective of this study was to examine the effect of different levels of catfish bone paste to flour on the physicochemical, textural and crumb structure properties of steamed bread. Six different levels (0, 1, 3, 5, 7,10 %) of catfish bone paste to flour were used in the formulation of the steamed bread. The results showed that the weight loss and TTA of steamed bread decreased with an increase in the levels of the catfish bone paste. On the other hand, the pH increased with an increase in the levels of the catfish bone paste. The specific volume, hardness, chewiness and gas cell structure in the crumb of steamed bread with catfish bone paste at 5% supplementation level were better. Thus, a value of 5% catfish bone paste was considered a better level for incorporation into the steamed bread.


2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ayssar Nahlé ◽  
Ideisan I. Abu-Abdoun ◽  
Ibrahim Abdel-Rahman

The inhibition and the effect of temperature and concentration of trans-4-hydroxy-4′-stilbazole on the corrosion of mild steel in 1 M HCl solution was investigated by weight loss experiments at temperatures ranging from 303 to 343 K. The studied inhibitor concentrations were between  M and  M. The percentage inhibition increased with the increase of the concentration of the inhibitor. The percentage inhibition reached about 94% at the concentration of  M and 303 K. On the other hand, the percentage inhibition decreased with the increase of temperature. Using the Temkin adsorption isotherm, the thermodynamic parameters for the adsorption of this inhibitor on the metal surface were calculated. Trans-4-hydroxy-4′-stilbazole was found to be a potential corrosion inhibitor since it contained not only nitrogen and oxygen, but also phenyl and pyridine rings that are joined together with a double bond (–C=C–) in conjugation with these rings.


1976 ◽  
Vol 38 (1) ◽  
pp. 191-198 ◽  
Author(s):  
D. C. Murray

Of 12 overweight women half received 10 wk. of self-control training and the rest received an equal period aimed at increasing determination to lose weight. Half of each treatment group had expressed a preference for the type of treatment they received and half for the other type of treatment. Both groups lost a statistically significant amount of weight, and at a 3-mo. follow-up there was still a significant weight loss. Follow-up at 6 mo. on 9 of the 12 original subjects indicated both groups regained much of their lost weight. There was no evidence that either type of treatment or receiving one's preferred type of treatment was related to weight loss.


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