Molecular characterization of Polychromophilus parasites of Scotophilus kuhlii bats in Thailand

Abstract Parasites of the haemosporidian genus Polychromophilus have exclusively been described in bats. These parasites belong to the diverse group of malaria parasites, and Polychromophilus presents the only haemosporidian taxon that infects mammalian hosts in tropical as well as in temperate climate zones. This study provides the first information of Polychromophilus parasites in the lesser Asiatic yellow bat (Scotophilus kuhlii) in Thailand, a common vespertilionid bat species distributed in South and Southeast Asia. The gametocyte blood stages of the parasites could not be assigned to a described morphospecies and molecular analysis revealed that these parasites might represent a distinct Polychromophilus species. In contrast to Plasmodium species, Polychromophilus parasites do not multiply in red blood cells and, thus, do not cause the clinical symptoms of malaria. Parasitological and molecular investigation of haemosporidian parasites of wildlife, such as the neglected genus Polychromophilus, will contribute to a better understanding of the evolution of malaria parasites.

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Molecular Characterization of Human Atypical Sorbitol-Fermenting Enteropathogenic Escherichia coli O157 Reveals High Diversity

Journal of Clinical Microbiology ◽

10.1128/jcm.02897-15 ◽

2016 ◽

Vol 54 (5) ◽

pp. 1357-1363 ◽

Cited By ~ 6

Author(s):

Annelene Kossow ◽

Wenlan Zhang ◽

Martina Bielaszewska ◽

Sophie Rhode ◽

Kevin Hansen ◽

...

Keyword(s):

Escherichia Coli ◽

Clinical Symptoms ◽

Diverse Group ◽

Full Spectrum ◽

Content Type ◽

E Coli ◽

Uremic Syndrome ◽

Encoding Gene ◽

Encoding Genes

Alongside the well-characterized enterohemorrhagicEscherichia coli(EHEC) O157:H7, serogroup O157 comprises sorbitol-fermenting typical and atypical enteropathogenicE. coli(EPEC/aEPEC) strains that carry the intimin-encoding geneeaebut not Shiga toxin-encoding genes (stx). Since little is known about these pathogens, we characterized 30 clinical isolates from patients with hemolytic uremic syndrome (HUS) or uncomplicated diarrhea with respect to their flagellin gene (fliC) type and multilocus sequence type (MLST). Moreover, we applied whole-genome sequencing (WGS) to determine the phylogenetic relationship with othereae-positive EHEC serotypes and the composition of therfbO157 region.fliCtyping resulted in fivefliCtypes (H7, H16, H34, H39, and H45). Isolates of eachfliCtype shared a unique ST. In comparison to the 42 HUS-associatedE. coli(HUSEC) strains, only thestx-negative isolates withfliCH7 shared their ST with EHEC O157:H7/H−strains. With the exception of one O157:H−fliCH16isolate, HUS was exclusively associated withfliCH7. WGS corroborated the separation of thefliCH7 isolates, which were closely related to the EHEC O157:H7/H−isolates, and the diverse group of isolates exhibiting differentfliCtypes, indicating independent evolution of the different serotypes. This was also supported by the heterogeneity within therfbO157 region that exhibited extensive recombinations. The genotypic subtypes and distribution of clinical symptoms suggested that thestx-negative O157 strains withfliCH7 were originally EHEC strains that loststx. The remaining isolates form a distinct and diverse group of atypical EPEC isolates that do not possess the full spectrum of virulence genes, underlining the importance of identifying the H antigen for clinical risk assessment.

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In vitro characterization of engineered red blood cells as viral traps against HIV-1 and SARS-CoV-2

Molecular Therapy — Methods & Clinical Development ◽

10.1016/j.omtm.2021.03.003 ◽

2021 ◽

Vol 21 ◽

pp. 161-170 ◽

Cited By ~ 1

Author(s):

Magnus A.G. Hoffmann ◽

Collin Kieffer ◽

Pamela J. Bjorkman

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

In Vitro Characterization ◽

Hiv 1

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In Vitro Assay for Single-cell Characterization of Impaired Deformability in Red Blood Cells under Recurrent Episodes of Hypoxia

Lab on a Chip ◽

10.1039/d1lc00598g ◽

2021 ◽

Author(s):

YUHAO QIANG ◽

Jia Liu ◽

Ming Dao ◽

E Du

Keyword(s):

Shear Stress ◽

Red Blood Cells ◽

Single Cell ◽

Blood Cells ◽

Blood Circulation ◽

In Vitro Assay ◽

Vitro Assay ◽

Cyclic Shear

Red blood cells (RBCs) are subjected to recurrent changes in shear stress and oxygen tension during blood circulation. The cyclic shear stress has been identified as an important factor that...

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Characterization of shear stress preventing red blood cells aggregation at the individual cell level: The temperature dependence

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-168020 ◽

2017 ◽

Vol 64 (4) ◽

pp. 853-857 ◽

Cited By ~ 11

Author(s):

K. Lee ◽

A. Priezzhev ◽

S. Shin ◽

F. Yaya ◽

I. Meglinski

Keyword(s):

Shear Stress ◽

Red Blood Cells ◽

Temperature Dependence ◽

Blood Cells ◽

Individual Cell ◽

Cell Level ◽

The Individual

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Characterization of Chemokine Receptors Expressed in Primitive Blood Cells During Human Hematopoietic Ontogeny

Stem Cells ◽

10.1634/stemcells.18-5-374 ◽

2000 ◽

Vol 18 (5) ◽

pp. 374-381 ◽

Cited By ~ 27

Author(s):

M. Rosu-Myles ◽

M. Khandaker ◽

D. M. Wu ◽

M. Keeney ◽

S. R. Foley ◽

...

Keyword(s):

Blood Cells ◽

Chemokine Receptors

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Molecular and chemical characterization of blood cells by infrared spectroscopy: A new optical tool in hematology

Blood Cells Molecules and Diseases ◽

10.1016/j.bcmd.2005.06.009 ◽

2005 ◽

Vol 35 (3) ◽

pp. 404-412 ◽

Cited By ~ 31

Author(s):

Kan-Zhi Liu ◽

Min-Hua Shi ◽

Henry H. Mantsch

Keyword(s):

Infrared Spectroscopy ◽

Blood Cells ◽

Chemical Characterization

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Characterization of Microparticles and Exosomes from Red Blood Cells During Storage: Implications for Transfusion Toxicity

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2016.10.527 ◽

2016 ◽

Vol 100 ◽

pp. S193

Author(s):

Joo-Yeun Oh ◽

Xin Xu ◽

Kristopher Genschmer ◽

Ming Zhong ◽

Jindong Li ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells

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Genetic and Antigenic Characterization of Avian Avulavirus Type 6 (AAvV-6) Circulating in Canadian Wild Birds (2005–2017)

Viruses ◽

10.3390/v13040543 ◽

2021 ◽

Vol 13 (4) ◽

pp. 543

Author(s):

Tamiko Hisanaga ◽

Catherine Soos ◽

Nicola Lewis ◽

Oliver Lung ◽

Matthew Suderman ◽

...

Keyword(s):

Clinical Symptoms ◽

Fusion Gene ◽

Full Genome ◽

Genome Sequences ◽

Viral Shedding ◽

Genetic Groups ◽

Antigenic Characterization ◽

Specific Pathogen ◽

First Time

We describe for the first time the genetic and antigenic characterization of 18 avian avulavirus type-6 viruses (AAvV-6) that were isolated from wild waterfowl in the Americas over the span of 12 years. Only one of the AAvV-6 viruses isolated failed to hemagglutinate chicken red blood cells. We were able to obtain full genome sequences of 16 and 2 fusion gene sequences from the remaining 2 isolates. This is more than double the number of full genome sequences available at the NCBI database. These AAvV-6 viruses phylogenetically grouped into the 2 existing AAvV-6 genotype subgroups indicating the existence of an intercontinental epidemiological link with other AAvV-6 viruses isolated from migratory waterfowl from different Eurasian countries. Antigenic maps made using HI assay data for these isolates showed that the two genetic groups were also antigenically distinct. An isolate representing each genotype was inoculated in specific pathogen free (SPF) chickens, however, no clinical symptoms were observed. A duplex fusion gene based real-time assay for the detection and genotyping of AAvV-6 to genotype 1 and 2 was developed. Using the developed assay, the viral shedding pattern in the infected chickens was examined. The chickens infected with both genotypes were able to shed the virus orally for about a week, however, no significant cloacal shedding was detected in chickens of both groups. Chickens in both groups developed detectable levels of anti-hemagglutinin antibodies 7 days after infection.

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