Protein Deposition Rate of Hybrid Boars and its Measurement

Author(s):  
T.A. Van Lunen ◽  
D.J.A. Cole

The development of genotypes with high protein deposition rates (PDR) has brought into question previously accepted theories concerning growth, protein deposition and nutrient requirements of pigs. It is clear that the full potential for lean deposition of these new genotypes can only be realized if we adopt appropriate nutritional strategies. As a first step the work reported here was conducted to determine the protein deposition rate of highly selected hybrid boars and to evaluate nitrogen balance and carcass composition techniques for estimating PDR.

1995 ◽  
Vol 60 (2) ◽  
pp. 299-305 ◽  
Author(s):  
N. Oksbjerg ◽  
M. T. Sørensen

AbstractTwo experiments were conducted to examine the effects of dietary inclusion of an ephedrine (Eph)/caffeine (Caf) mixture on finishing (55 to 100 kg live weight) castrated male pigs. The first experiment was conducted as a dose-response study and showed that the mixture had an optimal effect on performance and carcass composition at a dietary inclusion level of approximately 56/560 mg/kg of Eph and Caf, respectively. In the second experiment, interactions between the compounds were studied by dietary inclusion of Eph (56 mg/kg) and Caf (560 mg/kg) separately or as a mixture (56/560 mg/kg). The mixture reduced food intake by 0·12 and improved the food: gain ratio by 0·08. Muscle protein deposition rate was increased proportionately by 0·25, lipid accretion rate was decreased proportionately by 0·27, and gross energetic efficiency of energy deposition decreased proportionately by 0·09. This resulted in a changed proportional carcass composition towards more muscle (0·08) and less fat (0·21) after treatment with the mixture. The reduction in food intake was sustained due to an interaction over the first 3 weeks of treatment changing to additive effects by the compounds. The increased muscle protein deposition rate after feeding the mixture was entirely due to Eph, while the decreased gross energetic efficiency of energy deposition was caused by Caf. On the other hand, both compounds contributed almost equally to the decrease in lipid accretion rate. These results show that an Eph/Caf mixture markedly improves performance and body composition in finishing castrated pigs.


Author(s):  
T.A. Van Lunen ◽  
D.J.A. Cole

With the development of pig genotypes having high N deposition rates, previously accepted nutrient requirement recommendations must be re-examined. Requirements for protein (assuming lysine represents ‘ideal’ protein) for conventional growing pigs have been in the range of .60 to .74 g lysine/MJ DE (Batterham, 1994) while recent work with hybrid boars has shown a positive growth and N deposition response to ratios of the order of 1.0 (Rao and McCracken, 1991). It is generally accepted that sex has an effect on N deposition rate (Campbell et al., 1990) and therefore possibly on nutrient requirements. There is little information available on the effect of sex on growth and N deposition rate of modern genotypes. This experiment was conducted to estimate the N deposition rate of highly selected boars, gilts and castrates from 25 to 90 kg liveweight when fed diets containing ratios from 0.4 to 1.4 g lysine/MJ DE.


Critical Care ◽  
2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Pierre Singer ◽  
Itai Bendavid ◽  
Ilana BenArie ◽  
Liran Stadlander ◽  
Ilya Kagan

Abstract Background and aims Combining energy and protein targets during the acute phase of critical illness is challenging. Energy should be provided progressively to reach targets while avoiding overfeeding and ensuring sufficient protein provision. This prospective observational study evaluated the feasibility of achieving protein targets guided by 24-h urinary nitrogen excretion while avoiding overfeeding when administering a high protein-to-energy ratio enteral nutrition (EN) formula. Methods Critically ill adult mechanically ventilated patients with an APACHE II score > 15, SOFA > 4 and without gastrointestinal dysfunction received EN with hypocaloric content for 7 days. Protein need was determined by 24-h urinary nitrogen excretion, up to 1.2 g/kg (Group A, N = 10) or up to 1.5 g/kg (Group B, N = 22). Variables assessed included nitrogen intake, excretion, balance; resting energy expenditure (REE); phase angle (PhA); gastrointestinal tolerance of EN. Results Demographic characteristics of groups were similar. Protein target was achieved using urinary nitrogen excretion measurements. Nitrogen balance worsened in Group A but improved in Group B. Daily protein and calorie intake and balance were significantly increased in Group B compared to Group A. REE was correlated to PhA measurements. Gastric tolerance of EN was good. Conclusions Achieving the protein target using urinary nitrogen loss up to 1.5 g/kg/day was feasible in this hypercatabolic population. Reaching a higher protein and calorie target did not induce higher nitrogen excretion and was associated with improved nitrogen balance and a better energy intake without overfeeding. PhA appears to be related to REE and may reflect metabolism level, suggestive of a new phenotype for nutritional status. Trial registration 0795-18-RMC.


2002 ◽  
Vol 25 (4) ◽  
pp. 261-268 ◽  
Author(s):  
R. Bellomo ◽  
H. K. Tan ◽  
S. Bhonagiri ◽  
I. Gopal ◽  
J. Seacombe ◽  
...  

Aims To study the effect of combined continuous veno-venous hemodiafiltration (CVVHDF) and high (2.5 g/kg/day) parenteral amino acid supplementation on nitrogen balance, amino acid losses and azotemic control in a cohort of patients with severe acute renal failure (ARF). Methods We administered 2.5 grams/kg/day of amino acids intravenously to seven critically ill patients with ARF. We obtained paired blood and ultrafiltrate (UF) samples (n=20) and calculated amino acid clearances and losses, nitrogen balance, protein catabolic rate and total nitrogen losses. Results The median total serum amino acid concentration was high at 5.2 mmol/L with particularly high concentrations of ornithine, lysine, and phenylalanine, but a low level of histidine. The median overall amino acid clearance was 18.6 ml/min (range: 12 to 29 ml/min). UF losses as percentage of administered dose were high for tyrosine (53.6 %) but low for methionine (3.0 %) and arginine (2.3 %). A positive nitrogen balance was achieved in 7 (35%) of the 20 study days with an overall median nitrogen balance of -1.8 g/day. Urea levels were maintained at a median of 26.6 mmol/L. Conclusions High protein intake increases the serum concentrations of most amino acids. Such protein supplementation, when coupled with CVVHDF, achieves a slightly negative overall nitrogen balance in extremely catabolic patients while still allowing adequate azotemic control.


2000 ◽  
Vol 51 (5) ◽  
pp. 531 ◽  
Author(s):  
F. R. Dunshea ◽  
D. K. Kerton ◽  
P. D. Cranwell ◽  
R. G. Campbell ◽  
B. P. Mullan ◽  
...  

Seventy 14-day-old male pigs that were either heavy (6.0 kg) or light (3.6 kg) for age were weaned into individual pens and fed 1 of 7 diets containing 9.2–21.0 g lysine/kg feed in a study designed to determine the effect of dietary lysine and weight on lysine requirements of pigs. Five pigs from each weight group were used to determine initial body composition. When pigs reached 10 kg they were slaughtered and empty body composition was analysed to determine protein tissue accretion rates. Protein deposition rate was not affected by weight at weaning but increased with dietary lysine content before reaching a plateau. The relationship between protein deposition (PD, g/day) and dietary lysine (L, g/kg) was described by 3 models. The rectilinear model, which had a linear ascending phase (PD = 4.84 + 1.948L, R2 = 0.935, P = 0.002) and a horizontal component representing maximum protein deposition rate of 32.3 g/day, revealed that maximum protein deposition occurred at 14.1 g lysine/kg. The quadratic function (PD = – 2.74 + 3.74L – 0.099L2 , R2 = 0.916, P = 0.003) provided an estimate of the lysine requirement of 14.9 g lysine/kg occurring at a point where PD reached 95% of the maximum protein deposition rate (32.5 g/day). Use of an asymptotic model (PD = 32.60 – 186 0.727L, R2 = 0.919, P = 0.003) provided an estimate of 14.9 g lysine/kg occurring at a point where PD reached 95% of the maximum protein deposition rate (32.6 g/day). These data indicate that early weaned pigs should be fed a highly digestible diet containing 14–15 g lysine/kg to maximise protein deposition.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 109-110
Author(s):  
Robert Goodband ◽  
Mariana Menegat ◽  
Kiah Gourley ◽  
Lori Thomas ◽  
Mike Tokach ◽  
...  

Abstract Improvements in genetic selection, nutrition, health, and management has led to continuous improvement in sow productivity over the past 10 to 20 years. Today’s sows are more prolific with greater number of pigs gestated, nursed, and weaned per litter than in the past. Modern genotype females are also faster growing and leaner than predecessors. These changes in reproductive performance and body composition alter nutrient requirements during gestation and lactation. Gestation nutrient requirements appear to be met with approximately 11 to 12 g/d of standardized ileal digestible (SID) lysine and 5,000 to 6,000 Kcal/d NE. Providing greater lysine intake increases protein deposition and sow weight gain and may reduce stillbirth rate; however, the magnitude of economic benefit provides marginal economic response. Increasing energy and amino acid intake the last 3 to 4 days pre-farrowing appears to be a better alternative to shorten farrowing duration, increase the number of pigs born alive, and their weaning weights, than providing extra feed from d 90 of gestation through farrowing. During lactation, the goal is still to increase feed intake to minimize sow weight loss. Lysine requirement estimates in lactation seem to vary from study to study possibly reflecting the degree of sow muscle and fat catabolism. A summary of several lactation studies predicts a daily lactation requirement of 27 g SID Lys from the diet with an additional 13 g mobilized from body tissue for each 1 kg of litter growth. From a feed management standpoint, the availability of equipment to provide ad libitum consumption has been a major benefit in lactation. In conclusion, the literature suggests there are many successful ways to feed sows in gestation, peri-partum, and lactation, indicating that today’s sows are extremely resilient to nutritional challenges.


2017 ◽  
Vol 73 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Ali Karpuz ◽  
Salih Colmekci ◽  
Hakan Kockar ◽  
Hilal Kuru ◽  
Mehmet Uckun

AbstractThe structural and corresponding magnetic properties of Ni/Cu films sputtered at low and high deposition rates were investigated as there is a limited number of related studies in this field. 5[Ni(10 nm)/Cu(30 nm)] multilayer thin films were deposited using two DC sputtering sources at low (0.02 nm/s) and high (0.10 nm/s) deposition rates of Ni layers. A face centered cubic phase was detected for both films. The surface of the film sputtered at the low deposition rate has a lot of micro-grains distributed uniformly and with sizes from 0.1 to 0.4 μm. Also, it has a vertical acicular morphology. At high deposition rate, the number of micro-grains considerably decreased, and some of their sizes increased up to 1 μm. The surface of the Ni/Cu multilayer deposited at the low rate has a relatively more grainy and rugged structure, whereas the surface of the film deposited at the high rate has a relatively larger lateral size of surface grains with a relatively fine morphology. Saturation magnetisation, Ms, values were 90 and 138 emu/cm3 for deposition rates of 0.02 and 0.10 nm/s, respectively. Remanence, Mr, values were also found to be 48 and 71 emu/cm3 for the low and high deposition rates, respectively. The coercivity, Hc, values were 46 and 65 Oe for the low and high Ni deposition rates, respectively. The changes in the film surfaces provoked the changes in the Hc values. The Ms, Mr, and Hc values of the 5[Ni(10 nm)/Cu(30 nm)] films can be adjusted considering the surface morphologies and film contents caused by the different Ni deposition rates.


2000 ◽  
Vol 609 ◽  
Author(s):  
W.M.M. Kessels ◽  
A.H.M. Smets ◽  
J.P.M. Hoefnagels ◽  
M.G.H. Boogaarts ◽  
D.C. Schram ◽  
...  

ABSTRACTFrom investigations on the SiH3 and SiH radical density and the surface reaction probability in a remote Ar-H2-SiH4 plasma, it is unambiguously demonstrated that the a-Si:H film quality improves significantly with increasing contribution of SiH3 and decreasing contribution of very reactive (poly)silane radicals. Device quality a-Si:H is obtained at deposition rates up to 100 Å/s for conditions where film growth is governed by SiH3 (contribution ∼90%) and where SiH has only a minor contribution (∼2%). Furthermore, for SiH3 dominated film growth the effect of the deposition rate on the a-Si:H film properties with respect to the substrate temperature is discussed.


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