Structural and chemical analysis by transmission and scanning Electron Microscopy of particles found in eyes of cataract patients

1995 ◽  
Vol 53 ◽  
pp. 710-711
Author(s):  
D. Barron
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Medical Center ◽  
The Body ◽  
Cataract Operation ◽  
X Ray ◽  
Selected Area Electron Diffraction ◽  
Long Time ◽  
Time Accumulation ◽  
Scanning Electron

There has been a problem associated with removal of cataracts noted by a number of ophthalmologists, in particular, from Dr. David George at the Madigan Army Medical Center in the state of Washington. Particles were found lodged in the eyes of patients after the cataract operation (see fig. 1). There was serious concern about the origin of the particles. Were they from the ultrasonic surgical head, the surgical blade, or were they from a long time accumulation of the metals found in the body? In an effort to find answers, Dr. George sent samples of the cataract tissue filtered through a Whatman filter to the U.S. Army Center for Health Promotion and Preventive Medicine (CHPPM) for analysis by transmission electron microscopy (TEM), scanning electron microscopy (STEM), x-ray elemental spectral analysis (EDAX), and selected area electron diffraction (SAED). The results were compared with the composition of sample particles from the ultrasonic head and the surgical blade. These particles were filed off the head and blade and secured to a formvar film stretched over a TEM grid. Each set of particles was then analyzed by TEM and a x-ray elemental spectrum was generated.

Composition and Microstructure of a Natural Nano-Structure Mineral: Six-Ring Rock

2011 ◽  
Vol 295-297 ◽  
pp. 869-872
Author(s):  
Qing Shan Li ◽  
Xin Wang ◽  
Jun Liu ◽  
Guang Zhong Xing
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Dispersive Spectrometer ◽  
The Body ◽  
X Ray Diffraction ◽  
Nano Structure ◽  
X Ray ◽  
Transmission Electron ◽  
Nano Size ◽  
Scanning Electron

Six-ring Rock is widely used as containers of water and additives to produce health care products. In this paper, the composition and microstructure of Six-ring Rock have been investigated by using scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, x-ray diffraction and other technologies. Results show that Six-ring Rock is composed of CaMg(CO3)2, SiO2 and KAlSi3O8. Fe atoms exist in CaMg(CO3)2 by replacing Mg atoms. Six-ring Rock shows nano-size lamellar and acerose microstructures on the surface, and nano-size monocrystals in the body. Six-ring Rock is a natural nano structure mineral.

Mercury intake by inflammatory phagocytes: in vivo cytology of mouse macrophages and neutrophils by X-ray elemental microanalysis coupled with scanning electron microscopy

2004 ◽  
Vol 23 (9) ◽  
pp. 447-453 ◽  
Author(s):  
Elisabete M Cunha ◽  
Maria João R Oliveira ◽  
Paula G Ferreira ◽  
Artur P Águas
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peritoneal Cavity ◽  
The Body ◽  
Air Pouch ◽  
X Ray ◽  
Elemental Microanalysis ◽  
Short Period ◽  
Scanning Electron

Phagocytes remove and store mercury (Hg) that enters the body. Macrophages and granulocytes respond in opposite ways to Hg: macrophages loose cell viability, and neutrophils become protected from apoptosis. We have investigated the cytology of early intake of Hg by macrophages and neutrophils after a short period (2-4 min) of in vivo exposure to HgCl2. The two types of phagocytes were attracted either to a subcutaneous air pouch or to the peritoneal cavity of BALB/c mice by in situ BSA injection. BSA caused, 72 hours later, inflammatory exudates where neutrophils (air-pouch cavity) or macrophages (peritoneal cavity) were the predominant cell type. A lethal dose of HgCl2 (25 mg) was then injected in the two inflammatory cavities. The mice died 2-4 min later and the cell exudates were harvested and studied by scanning electron microscopy coupled with X-ray elemental microanalysis (SEM-XRM). More than half of the phagocytes showed ingested Hg; a higher percentage of macrophages (around 70%) than neutrophils (around 50%) were positive for the metal. Intracellular particles of Hg were spheroid and presented a small diameter (less than 20 nm). They could be seen in large numbers inside phagocytes (up to 20-30 Hg dots per cell); they were scattered throughout the cytoplasm of the cells. The ability of phagocytes to ingest Hg increased as the BSA-induced inflammation progressed. We conclude that (i) Hg is quickly ingested as small particles by phagocytes; (ii) endocytosis of Hg increases with the degree of activation of phagocytes; and (iii) phagocytes internalize Hg by pinocytosis.

scholarly journals Characterization of Orthorhombicα-MoO3Microplates Produced by a Microwave Plasma Process

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Arrak Klinbumrung ◽  
Titipun Thongtem ◽  
Somchai Thongtem
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microwave Plasma ◽  
Vibration Modes ◽  
X Ray Diffraction ◽  
Plasma Process ◽  
X Ray ◽  
Selected Area Electron Diffraction ◽  
Scanning Electron

Orthorhombic α-MoO3microplates were produced from (NH4)6Mo7O24·4H2O solid powder by a 900 W microwave plasma for 40, 50, and 60 min. Phase, morphologies, and vibration modes were characterized by X-ray diffraction (XRD), selected area electron diffraction (SAED), scanning electron microscopy (SEM), and Raman and Fourier transform infrared (FTIR) spectroscopy. Sixty min processing resulted in the best crystallization of the α-MoO3phase, with photoluminescence (PL) in a wavelength range of 430–440 nm.

The use of scanning electron microscopy and energy dispersive X-ray spectroscopy in a case of occupational death

2020 ◽  
Vol 88 (3) ◽  
pp. 163-168
Author(s):  
I Aquila ◽  
S Boca ◽  
P Ricci ◽  
G Perozziello ◽  
P Candeloro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cerebral Haemorrhage ◽  
The Body ◽  
Energy Dispersive ◽  
Legal Responsibility ◽  
X Ray ◽  
Work Related ◽  
High Resolution Images ◽  
Scanning Electron

Scanning electron microscopy is a technique that provides high-resolution images at the micro- and nano-scale. The combination of scanning electron microscopy and energy dispersive X-ray spectroscopy analysis is developing fast for application in forensic science. In this work, we report a case of work-related traumatic death of a 50-year-old man. The autopsy showed cranial fractures with cerebral haemorrhage. It was more difficult to understand the accident dynamics because the body had been shifted from the accident site to mask what had really taken place. Scanning electron microscopy/energy dispersive X-ray spectroscopy was used to identify the material of the impacting tool and to establish the possible legal responsibility of the employer. In this study, we demonstrate that scanning electron microscopy/energy dispersive X-ray spectroscopy is a useful forensic tool for the analysis of biological samples. Further, for studying the lacerations on the corpse from doubtful blunt tools, scanning electron microscopy/energy dispersive X-ray spectroscopy can assist in demonstrating that the scene has been falsified, as it was in this case.

scholarly journals Nanomaterials modify the growth of crops and some characteristics of organisms from agricultural or forest soils: An experimental study at laboratory, greenhouse and land level

2019 ◽  
Vol 4 (4) ◽  
pp. 29-49 ◽  
Author(s):  
Andrea Pérez-Moreno ◽  
Cesar Roberto Sarabia-Castillo ◽  
Gabriela Medina-Pérez ◽  
Hermes Pérez-Hernández ◽  
Jorge Roque De La Puente ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Common Bean ◽  
Cross Sections ◽  
Forest Soils ◽  
Ultra Performance Liquid Chromatography ◽  
The Body ◽  
Infrared Spectrometry ◽  
X Ray ◽  
Scanning Electron

Currently, some concerns regarding the potential toxicity of nanoparticles (NP) on the environment have emerged. The effect of ZnO, TiO2, and Fe2O3 NP on corn (Zea mays L.), common beans (Phaseolus vulgaris L.), nanobioremediation of polycyclic aromatic hydrocarbons (PAH), and soil organisms from agricultural or forest soils was studied at laboratory, greenhouse, and land level. The samples were analyzed by X-ray diffraction (XRD), field emission scanning electron microscopy with X-ray energy dispersion spectrometry (FESEM-EDS), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) gas chromatography (GC), ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) and Fourier transform infrared spectrometry with attenuated total reflectance (FTIR-ATR). ZnO-NP did not harm the mycorrhizal root colonization but, the presence of ZnO-NP decreased the degradation of PAH. The synthesis of metabolites from corn was more affected by the PAH than by ZnONP. FTIR spectra showed that NP affected the synthesis of compounds from specific functional groups in common bean plants. Fe2O3-NP were attached to the body of forestsoil organisms and significantly increased the concentration of Fe in their body, while TiO2-NP changed the morphological tissue of roots and stems of common bean as witnessed by micrographs of longitudinal and cross-sections. The NP used in this research significantly changed some response variables on the experiments carried-out at laboratory, greenhouse, and land level.

scholarly journals Structural analysis of a Nb-based alloy for biomedical application

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
JR. Severino Martins Jr ◽  
CR. Grandini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Crystalline Structure ◽  
Melting Furnace ◽  
The Body ◽  
Body Centered Cubic ◽  
X Ray Diffraction ◽  
Toxic Response ◽  
X Ray ◽  
Scanning Electron

The purpose of research in the biomaterials field is to produce new materials with physical and chemical properties close to the tissue to be replaced with minimal toxic response to the foreign body. Among the various metallic materials, titanium and its alloys have this great combination of properties. The most promising alloys are those with niobium, molybdenum, tantalum, and zirconium as alloying elements added to titanium. Thus, this kind of alloys integrate a new class of alloys without aluminum and vanadium (which cause cytotoxicity) and have a low modulus of elasticity (below 100 GPa). The objective of this work is to analyze the structure and microstructure of a niobium-based alloy, Ti-50wt%Nb. This alloy was produced in an arc-melting furnace with an inert atmosphere of argon gas. After melting, the samples were characterized by density, X-ray diffraction, scanning electron microscopy, and hardness. The X-ray diffraction data shows the peaks corresponding to the beta phase (with body-centered cubic crystalline structure), corroborated by scanning electron microscopy images. The value of the lattice parameter of the body-centered cubic crystalline structure was 3.2868 Å.  

Biomolecule-Assisted Synthesis of Ribbon-Like Sb2S3 Nanostructure

2010 ◽  
Vol 97-101 ◽  
pp. 2144-2147
Author(s):  
Xin Yu Yang ◽  
Wei Dong Xiang ◽  
Xi Yan Zhang ◽  
Hai Tao Liu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Sulfur Source ◽  
X Ray ◽  
Selected Area Electron Diffraction ◽  
Transmission Electron ◽  
High Resolution Tem ◽  
Scanning Electron

L-Cystine was successfully used as a novel kind of sulfur source to synthesize the nanostructure of ribbon-like Sb2S3. The nanoribbons were usually tens of micronmeters in length, typically 100-300 nm in width. The nanostructure of ribbon-like Sb2S3 were examined using diverse techniques including X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), selected-area electron diffraction, and high-resolution TEM.

Ultrastructural Analysis of Retrieved Ceramic Ball Heads

2003 ◽  
Vol 13 (4) ◽  
pp. 224-228
Author(s):  
G. Maccauro ◽  
L. Pilloni ◽  
L. Petrella ◽  
L. Proietti ◽  
V. De Santis ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hip Replacement ◽  
Ultrastructural Analysis ◽  
Ceramic Structure ◽  
Ceramic Ball ◽  
X Ray ◽  
Essential Condition ◽  
Long Time ◽  
Scanning Electron

Surface modification of alumina heads retrieved for aseptic loosening (6 cases) has been analysed using scanning electron microscopy, coupled with energy dispersive X-ray microanalysis. Ultrastructural analysis showed the presence of voids with grain detachment on all the retrieved cups and also in those cases in which macroscopic signs of wear were absent. The preservation of the macroscopic integrity of ceramic structure is an essential condition for long-time survival of total hip replacement, even if ceramic may also play a role in PE damage for the abrasive effects of grains. Macroscopic wear observed in two cases is not related to degradation of ceramic material but to other factors connected to surgery.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

Scanning Electron Microscopy and X-Ray Analyses of Dental Tissues from a Hibernator

1976 ◽  
Vol 34 ◽  
pp. 178-179
Author(s):  
M. L. Zimny ◽  
A. C. Haller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Temperature ◽  
Ground Squirrel ◽  
Alveolar Bone ◽  
X Ray ◽  
Dental Tissues ◽  
Bone Minerals ◽  
Apical Tooth ◽  
Scanning Electron

During hibernation the ground squirrel is immobile, body temperature reduced and metabolism depressed. Hibernation has been shown to affect dental tissues varying degrees, although not much work has been done in this area. In limited studies, it has been shown that hibernation results in (1) mobilization of bone minerals; (2) deficient dentinogenesis and degeneration of alveolar bone; (3) presence of calculus and tears in the cementum; and (4) aggrevation of caries and pulpal and apical tooth abscesses. The purpose of this investigation was to study the effects of hibernation on dental tissues employing scanning electron microscopy (SEM) and related x-ray analyses.

Sign in / Sign up

Export Citation Format

Share Document