scholarly journals Toxoplasma gondiiseroprevalence in wild boars (Sus scrofa) in Sweden and evaluation of ELISA test performance

2014 ◽  
Vol 143 (9) ◽  
pp. 1913-1921 ◽  
Author(s):  
C. WALLANDER ◽  
J. FRÖSSLING ◽  
I. VÅGSHOLM ◽  
A. UGGLA ◽  
A. LUNDÉN
Keyword(s):  
Test Performance ◽  
Latent Class ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Serum Samples ◽  
Wild Boars ◽  
Wide Range ◽  
South Sweden ◽  
Spatio Temporal ◽  
Set Up

SUMMARYToxoplasma gondiiis a zoonotic protozoan parasite, infecting a wide range of warm-blooded animals. The Swedish wild boar population is expanding and increased hunting provides its meat to a growing group of consumers. We performed a spatio-temporal investigation ofT. gondiiseroprevalence in Swedish wild boars. An ELISA was set up and evaluated against a commercial direct agglutination test, using Bayesian latent class analysis. The ELISA sensitivity and specificity were estimated to 79% and 85%, respectively. Of 1327 serum samples, 50% were positive. Thirty-four per cent of young wild boars and 55% of adults were positive (P< 0·001). The total seroprevalence ranged from 72% in 2005 to 38% in 2011 (P< 0·001), suggesting a declining trend. The highest seroprevalence, 65%, was recorded in South Sweden. In other regions it varied from 29% in Stockholm to 46% in East Middle Sweden.

scholarly journals Enzyme Immunoassay Detection of Antigen-Specific Immunoglobulin G Antibodies in Longitudinal Serum Samples from Patients with Cryptosporidiosis

2001 ◽  
Vol 8 (2) ◽  
pp. 415-423 ◽  
Author(s):  
Jeffrey W. Priest ◽  
Anna Li ◽  
Mohamad Khan ◽  
Michael J. Arrowood ◽  
Patrick J. Lammie ◽  
...  
Keyword(s):  
Western Blot ◽  
Immunoglobulin G ◽  
Protozoan Parasite ◽  
Epidemiologic Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igg Antibody ◽  
Large Format ◽  
Western Blot Assay ◽  
Wide Range

ABSTRACT Cryptosporidium parvum is a protozoan parasite that causes diarrheal illness in a wide range of mammalian hosts, including humans. Characteristic serum immunoglobulin G (IgG) antibody responses to antigens in the 27- and 17-kDa size ranges have been shown to develop after infection, and several enzyme-linked immunosorbent assay (ELISA) and Western blot assay formats have been used to measure these IgG levels in human serum. Using a collection of serial samples from laboratory-confirmed cryptosporidiosis patients, we compared the results obtained by using two new ELISAs with those obtained with two different Western blot assays. When assayed with the large-format Western blot, 97% of the 67 patients had a demonstrable antibody response on at least one occasion. The Cp23 ELISA correctly identified 93% of the samples that had a 27-kDa response by Western blot and 100% of the negative samples. The Triton antigen ELISA detected 77% of the samples that had a 17-kDa response by Western blot and 88% of the negative samples. The sensitivity of the Triton antigen assay was higher for samples collected between 16 and 92 days after the onset of symptoms (96%). The minigel-format Western blot did not compare favorably with the large-format blot for the detection of antibodies to the 27-kDa antigen (71% sensitivity). A half-life of about 12 weeks was estimated for antibodies to both the 27- and 17-kDa antigens. We believe the Cp23 and Triton antigen ELISAs will be useful in epidemiologic studies of the prevalence ofCryptosporidium infection in the population.

Sero-prevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic Zones of Himachal Pradesh, India

2021 ◽  
Author(s):  
Diksha Sharma ◽  
Snehil Gupta ◽  
Khushboo Sethi ◽  
Sanjay Kumar ◽  
RAJENDER KUMAR
Keyword(s):  
Control Strategies ◽  
Protozoan Parasite ◽  
Trypanosoma Evansi ◽  
Himachal Pradesh ◽  
Serum Samples ◽  
Climatic Zones ◽  
Lower Altitude ◽  
Whole Cell Lysate ◽  
Glucose Levels ◽  
Wide Range

Abstract Trypanosoma evansi, a hemoflagellate protozoan parasite causes wasting disease called surra in wide range of animals. Although, the organism has been reported from various parts of the India, data generated from organized epidemiological study is still in infancy in majority states of India. In the present study, livestock of Himachal Pradesh, India was targeted for epidemiological investigation of T. evansi infections. A total of 440 equines and 444 cattle serum samples were collected from four agro-climatic Zones. Further, serum samples of 280 buffaloes from three different agro-climatic Zones of Himachal Pradesh were also collected and evaluated for presence of T. evansi infection by indirect ELISA. Data generated showed higher prevalence in buffalo (23.57%) followed by cattle (22.52%) and equines (1.82%). Disease was found to be more prevalent (P < 0.05) in cattle of lower altitude as compared to those of higher altitudes. No significant variation was seen in prevalence of disease on the basis of age and sex of the animals. Serum biochemical analysis revealed increased levels of BUN in T. evansi infected equines. Levels of liver function enzymes such as ALT/GGT and AST were found to be significantly elevated (P < 0.01) in infected animals whereas glucose levels were significantly lower in surra infected animals as compared to non-infected animals. Western blot analysis of whole cell lysate (WCL) antigen of T. evansi using surra infected serum samples of equines showed immunodominant bands in the range of 100-25 kDa. Surra infected bovine serum samples recognized polypeptide bands in the range of 85-32 kDa, including protein clusters of 52-55 and 48-46 kDa. Poypeptide cluster of 62-66 kDa was found common to serum samples of bovines and equines from all agro-climatic Zones. Animal trypanosomosis was found to be highly prevalent in livestock of Himachal Pradesh and thus there is dire need for designing of proper control strategies against surra.

scholarly journals A network approach for provisional assay recognition of a Hendra virus antibody ELISA: test validation with low sample numbers from infected horses

2018 ◽  
Vol 30 (3) ◽  
pp. 362-369 ◽  
Author(s):  
Axel Colling ◽  
Ross Lunt ◽  
Jemma Bergfeld ◽  
Leanne McNabb ◽  
Kim Halpin ◽  
...  
Keyword(s):  
Test Performance ◽  
Serum Antibody ◽  
Relative Sensitivity ◽  
Elisa Test ◽  
Hendra Virus ◽  
Test Validation ◽  
Serum Samples ◽  
Antibody Elisa ◽  
Almost All ◽  
Positive Results

Obtaining statistically sound numbers of sera from Hendra virus (HeV)-infected horses is problematic because affected individuals usually die or are euthanized before developing a serum antibody response. As a consequence, test validation becomes a challenge. Our approach is an extension of OIE principles for provisional recognition and included 7 validation panels tested across multiple laboratories that provided estimates for test performance characteristics. At a 0.4 S/P cutoff, 16 of 19 sera from HeV-infected horses gave positive results in the HeV soluble G, indirect ELISA (HeVsG iELISA; DSe 84.2% [95% CI: 60.4–96.6%]); 463 of 477 non-infected horse sera tested negative (DSp 97.1% [95% CI: 95.1–98.4%]). The HeVsG iELISA eliminated almost all false-positive results from the previously used HeV iELISA, with marginally decreased relative sensitivity. Assay robustness was evaluated in inter-laboratory and proficiency testing panels. The HeVsG iELISA is considered to be fit for purpose for serosurveillance and international movement of horses when virus neutralization is used for follow-up testing of positive or inconclusive serum samples.

The Role of Test Anticipation in the Link Between Performance-Approach Goals and Academic Achievement

2016 ◽  
Vol 75 (3) ◽  
pp. 123-132 ◽  
Author(s):  
Marie Crouzevialle ◽  
Fabrizio Butera
Keyword(s):  
Learning Strategies ◽  
Test Performance ◽  
Longitudinal Design ◽  
Study Strategies ◽  
High Achievers ◽  
Course Content ◽  
And Mathematics ◽  
Set Up ◽  
Approach Goals ◽  
Goal Endorsement

Abstract. Performance-approach goals (i.e., the desire to outperform others) have been found to be positive predictors of test performance, but research has also revealed that they predict surface learning strategies. The present research investigates whether the high academic performance of students who strongly adopt performance-approach goals stems from test anticipation and preparation, which most educational settings render possible since examinations are often scheduled in advance. We set up a longitudinal design for an experiment conducted in high-school classrooms within the context of two science, technology, engineering, and mathematics (STEM) disciplines, namely, physics and chemistry. First, we measured performance-approach goals. Then we asked students to take a test that had either been announced a week in advance (enabling strategic preparation) or not. The expected interaction between performance-approach goal endorsement and test anticipation was moderated by the students’ initial level: The interaction appeared only among low achievers for whom the pursuit of performance-approach goals predicted greater performance – but only when the test had been scheduled. Conversely, high achievers appeared to have adopted a regular and steady process of course content learning whatever their normative goal endorsement. This suggests that normative strivings differentially influence the study strategies of low and high achievers.

Settlers or Movers? The Temporality of Past Migrations, Political Inaction and its Consequences, 1945–1985

2018 ◽  
Author(s):  
Jozefien De Bock
Keyword(s):  
Temporary Workers ◽  
Guest Workers ◽  
Temporary Migration ◽  
Wide Range ◽  
Settler Societies ◽  
Future Policy ◽  
Set Up ◽  
Legal Frameworks ◽  
Temporary Migrants ◽  
Multicultural Policies

Historically, those societies that have the longest tradition in multicultural policies are settler societies. The question of how to deal with temporary migrants has only recently aroused their interest. In Europe, temporary migration programmes have a much longer history. In the period after WWII, a wide range of legal frameworks were set up to import temporary workers, who came to be known as guest workers. In the end, many of these ‘guests’ settled in Europe permanently. Their presence lay at the basis of European multicultural policies. However, when these policies were drafted, the former mobility of guest workers had been forgotten. This chapter will focus on this mobility of initially temporary workers, comparing the period of economic growth 1945-1974 with the years after the 1974 economic crisis. Further, it will look at the kind of policies that were developed towards guest workers in the era before multiculturalism. This way, it shows how their consideration as temporary residents had far-reaching consequences for the immigrants, their descendants and the receiving societies involved. The chapter will finish by suggesting a number of lessons from the past. If the mobility-gap between guest workers and present-day migrants is not as big as generally assumed, then the consequences of previous neglect should serve as a warning for future policy making.

ORAL HISTORY SOURCES AS LEARNING MATERIALS: A CASE STUDY OF THE NATIONAL UNIVERSITY OF SCIENCE AND TECHNOLOGY

2016 ◽  
Vol 3 (2) ◽  
pp. 82-93
Author(s):  
Gugulethu Shamaine Nkala ◽  
Rodreck David
Keyword(s):  
Oral History ◽  
Learning Experience ◽  
Science And Technology ◽  
Group Discussion ◽  
Human Interaction ◽  
Learning Material ◽  
Wide Range ◽  
National Archives ◽  
National University ◽  
Set Up

Knowledge presented by Oral History (OH) is unique in that it shares the tacit perspective, thoughts, opinions and understanding of the interviewee in its primary form. While teachers, lecturers and other education specialists have at their disposal a wide range of primary, secondary and tertiary sources upon which to relate and share or impart knowledge, OH presents a rich source of information that can improve the learning and knowledge impartation experience. The uniqueness of OH is presented in the following advantages of its use: it allows one to learn about the perspectives of individuals who might not otherwise appear in the historical record; it allows one to compensate for the digital age; one can learn different kinds of information; it provides historical actors with an opportunity to tell their own stories in their own words; and it offers a rich opportunity for human interaction. This article discusses the placement of oral history in the classroom set-up by investigating its use as a source of learning material presented by the National Archives of Zimbabwe to students in the Department of Records and Archives Management at the National University of Science and Technology (NUST). Interviews and a group discussion were used to gather data from an archivist at the National Archives of Zimbabwe, lecturers and students in the Department of Records and Archives Management at NUST, respectively. These groups were approached on the usability, uniqueness and other characteristics that support this type of knowledge about OH in a tertiary learning experience. The findings indicate several qualities that reflect the richness of OH as a teaching source material in a classroom set-up. It further points to weak areas that may be addressed where the source is considered a viable strategy for knowledge sharing and learning. The researchers present a possible model that can be used to champion the use of this rich knowledge source in classroom education at this university and in similar set-ups. 

MONITORING STUDIES OF ARBOVIRUS INFECTIONS TRANSMITTED BY MOSQUITOES ON THE TERRITORY OF THE VOLGOGRAD REGION

2019 ◽  
pp. 60-66
Author(s):  
E.V. Molchanova ◽  
D.N. Luchinin ◽  
A.O. Negodenko ◽  
D.R. Prilepskaya ◽  
N.V. Boroday ◽  
...  
Keyword(s):  
Virus Antigen ◽  
Elisa Test ◽  
Serum Samples ◽  
Blood Sucking ◽  
Tick Borne Encephalitis ◽  
Volgograd Region ◽  
Two Samples ◽  
Probable Presence ◽  
California Serogroup ◽  
Tick Borne Encephalitis Virus

The paper presents data from the monitoring studies’ results of arbovirus infections transmitted by mosquitoes in the Volgograd region. West Nile virus antigen (WNV) in 9 samples, Tahyna virus in one sample, Batai virus in two samples were detected in the study of 110 samples of field material (blood-sucking mosquitoes) by ELISA test. Antibodies to WNV in 16.58 percent of the samples, to tick-borne encephalitis virus in 1.08 percent, to viruses of the California serogroup and Ukuniemi in 1.09 percent, to the virus Sindbis in 2.17 percent were detected as a result of the study of blood serum samples from donors in the Volgograd region. Thus, we obtained data on the probable presence of the Batai, Sindbis, Ukuniemi and Californian serogroup viruses along with the circulation of WNV on the territory of the Volgograd region.

Nanofiltration of endocrine disrupting compounds

2003 ◽  
Vol 3 (5-6) ◽  
pp. 321-327 ◽  
Author(s):  
M. Gallenkemper ◽  
T. Wintgens ◽  
T. Melin
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Endocrine Disrupting Compounds ◽  
Municipal Wastewater Treatment ◽  
Water And Wastewater Treatment ◽  
Wide Range ◽  
Endocrine Disrupting ◽  
Municipal Wastewater Treatment Plants ◽  
Set Up

Endocrine disrupting compounds can affect the hormone system in organisms. A wide range of endocrine disrupters were found in sewage and effluents of municipal wastewater treatment plants. Toxicological evaluations indicate that conventional wastewater treatment plants are not able to remove these substances sufficiently before disposing effluent into the environment. Membrane technology, which is proving to be an effective barrier to these substances, is the subject of this research. Nanofiltration provides high quality permeates in water and wastewater treatment. Eleven different nanofiltration membranes were tested in the laboratory set-up. The observed retention for nonylphenol (NP) and bisphenol A (BPA) ranged between 70% and 100%. The contact angle is an indicator for the hydrophobicity of a membrane, whose influence on the permeability and retention of NP was evident. The retention of BPA was found to be inversely proportional to the membrane permeability.

Statistical Quality Control of HIV-1 ELISA Test Performance

1994 ◽  
Vol 89 (428) ◽  
pp. 1200-1208 ◽  
Author(s):  
R. C. Gentleman ◽  
M. S. Hamada ◽  
D. E. Matthews ◽  
A. R. Wilson
Keyword(s):  
Quality Control ◽  
Test Performance ◽  
Elisa Test ◽  
Statistical Quality Control ◽  
Statistical Quality ◽  
Hiv 1

scholarly journals Detection of Nipah virus in Pteropus medius in 2019 outbreak from Ernakulam district, Kerala, India

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. B. Sudeep ◽  
Pragya D. Yadav ◽  
Mangesh D. Gokhale ◽  
R. Balasubramanian ◽  
Nivedita Gupta ◽  
...  
Keyword(s):  
Index Case ◽  
Nipah Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Genotype I ◽  
Visceral Organs ◽  
Distinct Cluster ◽  
New Genotype ◽  
Kerala State ◽  
Set Up

Abstract Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection.

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