Phytohormone dynamics impact fatty acid and oil accumulation during soybean seed maturation

2021 ◽  
pp. 1-14
Author(s):  
Thien Q. Nguyen ◽  
Anna B. Kisiala ◽  
Nguyen Ngoc Hai ◽  
Suresh Narine ◽  
R. J. Neil Emery
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Hormonal Regulation ◽  
Oil Content ◽  
Oil Quality ◽  
Soybean Seed ◽  
Principal Component ◽  
Seed Maturation ◽  
Hormone Regulation ◽  
Oil Accumulation

Abstract Fatty acid (FA) levels and profiles are vital for soybean oil quality, while cytokinins (CKs) and abscisic acid (ABA) are potent regulators of plant growth and development. Previous research suggested associations between FA biosynthesis and hormonal signalling networks; however, hormonal regulation of FA accumulation during soybean (Glycine max) seed maturation has never been measured. We analysed hormone and FA profiles obtained from HPLC-(ESI)-MS/MS and GC-FID screening during soybean seed maturation. A multilayered data processing approach, involving heat-maps, principal component analysis (PCA), correlation and multiregression models, suggested a strong relationship between hormone metabolism and FA/oil accumulation during seed maturation. Most strikingly, positive correlations were found between the levels of CK ribosides [transZeatin riboside (tZR), N6-isopentenyladenosine (iPR)] at the early stages of SM (R5-R6) and C18:0, C18:2 and oil content at the R8 stage. Moreover, multiple regression models revealed functional linkages between several CK derivatives and FA and oil content in mature seeds. To further test the significance of hormone regulation in FA metabolism, plants of two soybean accessions with contrasting hormone and FA profiles were sprayed with exogenous ABA and transZeatin (tZ) during the seed-filling period (R5-R6). Depending on the hormone type and concentration, these treatments distinctly modified biosynthesis of all tested FAs, except for C18:0. Most remarkably, tZ (50 nM) promoted production of C16:0, C18:1, C18:2, C18:3, and oil accumulation in maturing seeds. Overall, the results indicate impactful roles for ABA and CKs in FA accumulation during SM and represent a further step towards understanding FA biosynthesis, and potential improvements of soybean oil profiles.

scholarly journals Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds

2019 ◽  
Vol 20 (9) ◽  
pp. 2202 ◽  
Author(s):  
Songnan Yang ◽  
Long Miao ◽  
Jianbo He ◽  
Kai Zhang ◽  
Yan Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Candidate Genes ◽  
Molecular Basis ◽  
Oil Content ◽  
Seed Oil ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Seed Oil Content ◽  
Oil Accumulation ◽  
Expression Levels

Soybean is one of the most important oil crops in the world. Revealing the molecular basis and exploring key candidate genes for seed oil synthesis has great significance for soybean improvement. In this study, we found that oil accumulation rates and gene expression levels changed dynamically during soybean seed development. The expression levels of genes in metabolic pathways such as carbon fixation, photosynthesis, glycolysis, and fatty acid biosynthesis were significantly up-regulated during the rapid accumulation of oil in developing soybean seeds. Through weighted correlation network analysis, we identified six co-expression modules associated with soybean seed oil content and the pink module was the most positively correlated (r = 0.83, p = 7 × 10−4) network. Through the integration of differential expression and co-expression analysis, we predicted 124 candidate genes potentially affecting soybean seed oil content, including seven genes in lipid metabolism pathway, two genes involved in glycolysis, one gene in sucrose metabolism, and 12 genes belonged to transcription factors as well as other categories. Among these, three genes (GmABI3b, GmNFYA and GmFAD2-1B) have been shown to control oil and fatty acid content in soybean seeds, and other newly identified candidate genes would broaden our knowledge to understand the molecular basis for oil accumulation in soybean seeds.

scholarly journals Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Karen Hudson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Large Scale ◽  
Oil Quality ◽  
Soybean Seed ◽  
Subsequent Generation ◽  
Oil Composition ◽  
Fatty Acid Profiling ◽  
Wide Range

To identify genetic variation for fatty acid composition in mature soybean seeds, 4566 M3generation seed samples from a chemically mutagenized population were subjected to fatty acid profiling by gas chromatography. In the population, a wide range of variation in the content for each of the five major fatty acids was observed. Seventy-nine lines were identified which contained significantly high or low levels of one of the five major soybean fatty acids. These lines were advanced to the subsequent generation. Of the 79 lines showing a variant fatty acid profile in the M3, 52 showed clear heritability for the oil composition in the seeds of the subsequent generation. These lines are likely to represent 52 distinct genetic mutations. These mutants may represent new loci involved in the determination of soybean seed oil content or could be new isolates or alleles of previously identified genetic variants for soybean oil composition.

scholarly journals Cold-pressed cactus pear seed oil: Quality and stability

2021 ◽  
Vol 72 (3) ◽  
pp. e415 ◽  
Author(s):  
M. De Wit ◽  
V.K. Motsamai ◽  
A. Hugo
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Physicochemical Properties ◽  
Acid Composition ◽  
Oil Content ◽  
Seed Oil ◽  
Oil Quality ◽  
Cactus Pear ◽  
Cold Pressed

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).

scholarly journals Selecting Yield and Nutritional Traits in Sphenostylis stenocarpa Landraces for Food Improvement

2020 ◽  
Vol 11 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Charity Aremu ◽  
Micheal Abberton ◽  
Timothy Adebiyi ◽  
Abiola J. Asaleye ◽  
Henry Inegbedion ◽  
...  
Keyword(s):  
Oil Content ◽  
Agricultural Sector ◽  
Oil Quality ◽  
Maximum Yield ◽  
Principal Component ◽  
Variation Patterns ◽  
Variance Table ◽  
Quality Of Food ◽  
Sphenostylis Stenocarpa

Background: Sphenostylis stenocarpa is an underexploited African indigenous food crop that is enriched in nutritional quality. Objective: Exploring the robust genetic base of this landrace can help to maximize the benefit of the agricultural sector on the economy through production that is enhanced by packaging and patent. This as well will increase the quality of food production and promote African campaign on food sustainability. Methods: Upon this, this research made use of multiple statistics to identify S. stenocarpa yield and nutritional trait relatedness that supported selection for maximum yield and nutritional trait output. Yield and related traits including protein and oil contents of twenty-three Sphenostylis stenocarpa landraces were studied under a four year planting seasons in Teaching and Research farm of Landmark University, Nigeria. Results: Trait variances from Landrace × Year (L × Y) interaction, Principal Component and Cluster analyses were evaluated and the variation patterns were identified. Some vegetative (maturity phase, height and branching) and yield traits (Pod traits, seed yield and oil content) correlated significantly (p < 0.05) in the L × Y interactions. This suggests the usefulness of these traits in improving S. stenocarpa grain and oil quality yield. Tuber and nodule yield including protein content did not differ significantly in the variance table. Conclusion: The result indicates that one location trial is insufficient to determine such trait performance. The first four PCs that accounted for 51 percent of the total variations were traceable to branching, maturity date, pod numbers, seed and oil content as main contributors to yield.

scholarly journals Identification of Key Genes Involved in Embryo Development and Differential Oil Accumulation in Two Contrasting Maize Genotypes

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 993 ◽  
Author(s):  
Xiangxiang Zhang ◽  
Meiyan Hong ◽  
Heping Wan ◽  
Lixia Luo ◽  
Zeen Yu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Embryo Development ◽  
Oil Content ◽  
Fatty Acid Biosynthesis ◽  
Developmental Stages ◽  
Enrichment Analysis ◽  
Sequencing Analysis ◽  
Oil Accumulation ◽  
Maize Genotypes ◽  
Maize Embryo

Maize is an important oil seed crop and a major food crop in different parts of the world. Since maize has relatively lower seed oil content as compared to other oil crops, efforts are continuing to improve its oil content percentage. In this study, we analyzed two contrasting maize genotypes with differential oil accumulation percentages. High oil-content (HOC) maize had 11% oil content while low oil-content (LOC) maize had significantly lower oil content (5.4%). Transmission electron microscopy revealed a higher accumulation of oil bodies in the HOC maize embryo as compared to LOC maize. Comparative RNA-sequencing analysis at different developmental stages of the seed embryos identified 739 genes that are constantly differentially expressed (DEGs) at all the six developmental stages from 15 days after pollination (DAP) to 40 DAP. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified fatty acid metabolism and fatty acid biosynthesis as the most enriched biological pathways contributed by these DEGs. Notably, transcriptional changes are more intense at the early stages of embryo development as compared to later stages. In addition, pathways related to oil biosynthesis and their corresponding genes were more enriched at 30 DAP, which seems to be the key stage for oil accumulation. The study also identified 33 key DEGs involved in fatty acid and triacylglycerols biosynthesis, most of which were up-regulated in HOC, that may shape the differential oil contents in the two contrasting maize. Notably, we discovered that both acyl-CoA-dependent and acyl-CoA-independent processes are essential for the high oil accumulation in maize embryo.

scholarly journals Response of Different Level of Nitrogen and Phosphorus on Grain Yield, Oil Quality and Nutrient Uptake of Soybean

1970 ◽  
Vol 44 (2) ◽  
pp. 187-192
Author(s):  
AA Jahangir ◽  
RK Mondal ◽  
K Nada ◽  
MAM Sarker ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Nutrient Uptake ◽  
Oil Content ◽  
Oil Quality ◽  
Soybean Seed ◽  
Nutrient Content ◽  
Single Application ◽  
Nitrogen And Phosphorus ◽  
Nitrogen Phosphorus ◽  
Different Levels ◽  
Key Words Nitrogen

A field experiment was conducted to find out the effect to different levels of nitrogen and phosphorus on yield, oil contents and quality and nutrient uptake of soybean. The maximum plant height and number of pod per plant were recorded by the application of 40kg N / ha. Single application of phosphorus also bring significant increased in oil content of soybean. Nitrogen, phosphorus, potash content of soybean seed and straw varied with different treatment of NP. Interaction showed oil content of soybean was influenced with increasing level of nitrogen and phosphorus. The percent of oil and yield of soybean were found maximized by the application of 40kg N / ha + 30kg P2O5 / ha. However, moisture, density, refractive index and peroxide value of soybean oil remain more or less same throughout the experiment. Key words: Nitrogen, Phosphorus, Oil quality, Nutrient content, Grain and Straw yield. DOI: 10.3329/bjsir.v44i2.3670 Bangladesh J. Sci. Ind. Res. 44(2), 187-192, 2009

scholarly journals Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

2014 ◽  
Vol 12 (7) ◽  
pp. 934-940 ◽  
Author(s):  
William Haun ◽  
Andrew Coffman ◽  
Benjamin M. Clasen ◽  
Zachary L. Demorest ◽  
Anita Lowy ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Gene Family ◽  
Oil Quality ◽  
Fatty Acid Desaturase ◽  
Targeted Mutagenesis ◽  
Fatty Acid Desaturase 2

scholarly journals Effect of Genotypes and Environments on Oil Content and Oil Quality Parameters and Their Correlation in Peanut (Arachis hypogaea L.)1

1993 ◽  
Vol 20 (2) ◽  
pp. 84-89 ◽  
Author(s):  
S. L. Dwivedi ◽  
S. N. Nigam ◽  
R. Jambunathan ◽  
K. L. Sahrawat ◽  
G. V. S. Nagabhushanam ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Arachis Hypogaea ◽  
Soil Ph ◽  
Oil Content ◽  
Oil Quality ◽  
Quality Parameters ◽  
Environment Interaction ◽  
Arachis Hypogaea L ◽  
Fe Content

Abstract The quality of edible oils is now receiving increasing consideration from consumers and processors. The present study was conducted to investigate the effects of environments on oil content and fatty acid composition in peanut. The correlation between oil content and oil quality parameters was also studied. Thirteen peanut (Arachis hypogaea L.) genotypes were grown in 12 environments for the study. Soils at experiment locations differed significantly for pH, EC, and N, P, Zn, Mn, and Fe contents. Significant genotype, environment, and genotype x environment interaction effects were observed for oil content, individual fatty acid contents, and derived oil quality parameters. The original range of 34-54% of oil content based on one season/location evaluation in these lines was not repeatable, and ranged from 45-50% in multilocation evaluation. Oil content was positively correlated with soil pH and Fe content. The correlation of oleic and linoleic acid content with soil pH and Fe content was positive in the former and negative in the latter. The oil content was positively correlated with O/L ratio. Oliec and linoleic acid contents were negatively correlated. Selection for reduced linoleic acid level in genotypes would also reduce levels of total long chain saturated fatty (TLCSF) acids. Of the thirteen genotypes tested, ICG 5856, ICG 5369, and ICGV 87124 could be used in breeding for improved oil quality.

Metribuzin Effects on Seed Constituents of Soybean Varieties

Weed Science ◽  
1974 ◽  
Vol 22 (6) ◽  
pp. 575-577 ◽  
Author(s):  
W. S. Hardcastle ◽  
R. E. Wilkinson ◽  
C. T. Young
Keyword(s):  
Glycine Max ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Acid Concentration ◽  
Total Fatty Acid ◽  
Oil Quality ◽  
Fatty Acid Concentration ◽  
Percent Protein

Metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazine-5(4H)one] (0, 0.56, 1.12, 1.68 kg/ha) applied the soybean (Glycine max (L.) Merr.) cultivars ‘Bragg,’ ‘Hampton,’ ‘Bienville,’ ‘Coker 318,’ ‘Coker 102,’ and ‘Hardee’ induced significant changes in total fatty acid concentration of soybean oil. Minor changes in soybean oil quality were induced by metribuzin but percent protein was not influenced.

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