Rumen microbial counts and in vivo digestibility in buffaloes and cattle given different diets

2002 ◽  
Vol 75 (2) ◽  
pp. 323-329 ◽  
Author(s):  
S. Puppo ◽  
S. Bartocci ◽  
S. Terramoccia ◽  
F. Grandoni ◽  
A. Amici
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Protein Digestibility ◽  
Rumen Content ◽  
Crude Protein Content ◽  
Bacterial Counts ◽  
Microbial Counts

AbstractRuminal bacterial counts and in vivo digestibility were determined on four Mediterranean buffalo bulls and four Friesian bulls, all fistulated at the rumen, and given at maintenance level (50 g/kg M0·75 per day of dry matter) four different diets with the same crude protein content (N ✕ 6·25 = 140 g/kg dry matter) and with forage: concentrate ratios as follows: diet D12·5 = 0·875: 0·125; diet D25·0 = 0·75: 0·25; diet D37·5 = 0·625: 0·375; diet D50·0 = 0·5: 0·5. All the animals received the diets during four consecutive periods in a Latin-square design. Buffaloes had higher total microbial counts (10·78 v. 10·08 log10cells per g dry rumen content, P < 0·01) as compared with cattle; differences in total ruminal bacterial counts among the diets were only observed within the buffalo species (diet D12·5 v. diets D25·0, D37·5, D50·0: 10·04 v. 10·92, 10·98, 11·17 log10cells per g dry rumen content, P 0·01) and when comparing the two species for each diet, significantly higher values for bacterial counts in buffaloes were found for diets D25·0: 10·92 v. 10·28 (P 0·05), D37·5: 10·98 v. 10·08 (P 0·01) and D50·0: 11·17 v. 9·76 (P 0·01) log10cells per g dry rumen content. Cattle showed significantly higher digestibility values for: organic matter (0·696 v. 0·676, P 0·05), neutral-detergent fibre (NDF; 0·548 v. 0·511, P 0·05) and cellulose (0·621 v. 0 · 509, P 0·01), while the crude protein digestibility (CPD) values were similar (0·667 and 0·671). Comparing the two species for each diet, cattle showed significantly higher digestibility values for organic matter in diet D50·0 only (0·714 v. 0·688, P 0·01), for NDF in diet D12·5 only (0·578 v. 0·531, P 0·05) and for cellulose in all diets (0·660 v. 0·546, 0·630 v. 0·525, 0·605 v. 0·505, 0·588 v. 0·460, P 0·01); in contrast buffaloes showed higher values of the CPD for diet D12·5 (0·662 v. 0·632, P 0·05).

THE EFFECTS OF ADVANCING MATURITY ON CRUDE PROTEIN AND DIGESTIBILITY OF MEADOW FOXTAIL (Alopecurus pratensis) AND TIMOTHY (Phleum pratense)

1983 ◽  
Vol 63 (4) ◽  
pp. 1083-1085 ◽  
Author(s):  
GWEN WALDIE ◽  
S. B. M. WRIGHT ◽  
R. D. H. COHEN
Keyword(s):  
British Columbia ◽  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Phleum Pratense ◽  
Crude Protein Content ◽  
Phleum Pratense L

The crude protein content (CP %) and in vivo digestibilities of dry matter (DMD %) and protein (PD %) were determined on freshly cut monocultures of timothy (Phleum pratense L.) and meadow foxtail (Alopecurus pratensis L.) from 19 June to 23 Aug. at Prince George, British Columbia. CP, DMD and PD declined significantly with advancing maturity for both grasses.Key words: Meadow foxtail, timothy, protein, digestibility

Intakes andin vivoapparent digestibilities of four types of conserved grass forage by ponies

2000 ◽  
Vol 71 (3) ◽  
pp. 527-534 ◽  
Author(s):  
M.J. S. Moore-Colyer ◽  
A. C. Longland
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Live Weight ◽  
High Energy ◽  
Digestible Energy ◽  
Faecal Samples ◽  
Gross Energy

AbstractThe intakes and in vivo apparent digestibilities (AD) of four forages: hay (H), haylage (HY), big-bale silage (BB) and clamp silage (CS), were investigated in Welsh cross pony geldings (live weight (LW) ca. 330 kg). The trial was a 4✕4 Latin-square design with diets offered at 1•65 g/kg dry matter (DM) per100 kg LW per day, in two equal meals. Food and faecal samples were analysed for DM, organic matter, crude protein (CP), gross energy, calcium (Ca), phosphorus (P), magnesium (Mg) and non-starch polysaccharide (NSP) composition and content. Significant differences (P < 0·05) in DM intakes (DMI) g/kg M0·75were recorded between foods, where HY = BB = H > CS. For all measurements AD was significantly (P < 0·05) higher for BB and CS than for H, with HY being intermediate in all but digestibilities of Ca and Mg. Digestibility of NSP of BB and CS were significantly (P < 0·05) greater than for H and HY. The digestible energy (DE) of H, HY, BB and CS were 5·75, 9·09, 9·83 and 11·98 MJ/kg DM respectively, which coupled with the corresponding DMI resulted in all foods but H meeting the theoretical daily DE requirements of the ponies. Similarly, theoretical digestible CP requirements were met on all diets except H. NSP fractions accounted for 0·5 of daily DE in both H and BB, which were significantly (P < 0·05) higher than for CS and HY at 0·4 and 0·2 respectively. These results indicate that CS, BB and HY are readily digested by ponies and thus offer suitable high-energy alternatives to hay in horse rations.

scholarly journals The influence of the method of preservation of forages on the digestion in dairy cows. 1. Composition of the forages and digestibility of dry matter, organic matter and nitrogen.

1975 ◽  
Vol 23 (1) ◽  
pp. 3-9
Author(s):  
S. Tamminga ◽  
C.J. van der Koelen
Keyword(s):  
Organic Matter ◽  
Formic Acid ◽  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Mixed Diet ◽  
Apparent Digestibility

1. Grass from the same sward was ensiled without additive, with 14.6 g formic acid/100 g crude protein or 10.8 g formic acid and 10.6 g formaldehyde/100 g crude protein. Similar grass was dried and pelleted. Drying or ensiling with the mixture reduced solubility of N in the preserved grass but formic acid increased it, and ensiling without additive increased it even more. Apparent digestibility of N in the rumen of cows tended to decrease with decrease in solubility. Digestibility in vitro of the mixed diet given to the cows, calculated from digestibility of the separate components, agreed well with the values in vivo for diets with silages, but was high for that with dried grass. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Development of yield and nutritive value of timothy in primary growth and regrowth in northern growing conditions

2008 ◽  
Vol 19 (3) ◽  
pp. 252 ◽  
Author(s):  
O. NISSINEN ◽  
P. KALLIAINEN ◽  
L. JAUHIAINEN
Keyword(s):  
Organic Matter ◽  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Rapid Decline ◽  
Crude Protein Content ◽  
Percentage Unit ◽  
Primary Growth ◽  
Organic Matter Digestibility

The development of the yield and nutritive value of timothy (Phleum pratense L.) both in the primary growth and in the regrowth were studied at MTT Plant Production Research, a unit of MTT Agrifood Research Finland, in Rovaniemi (66°35´N) in 1999–2001. The dry matter yield and leaf:stem ratio were measured from the crop samples, and the contents of crude protein and organic matter digestibility of both whole plant samples and leaf and stem fractions were analysed. In primary growth, the most rapid increase of dry matter, 220–240 kg ha-1 per day, was measured around the beginning of the heading stage. There was a very strong positive correlation between the proportion of stems and the amount of dry matter in the primary yield. The daily growth rate of the regrowth was less than half of that of the primary growth. The fastest decrease, 1 percentage unit per day, in crude protein content was measured at the pasture stage (4–5-leaf stage). During the entire sampling period, the average daily decline in crude protein content in the primary growth of timothy was 0.65 percentage units. The main cause for the rapid decline in crude protein content was the high proportion of stem matter and its low protein content. In the regrowth, during the last four weeks before the harvest, the average daily decline in crude protein content was 0.28 percentage units.The average decline in organic matter digestibility from early pasture stage to late silage stage was 0.9 percentage units per day. The most remarkable change was noticed at the growth stage of timothy when about the half of stems were heading and it was then that the digestibility decreased by more than one percentage unit per day. The rapid decline in organic matter digestibility was due to the low digestibility of stem matter. The daily change in forage digestibility in the regrowth was very small, on average 0.11%.;

scholarly journals Replacing grass silage with pea-barley intercrop silage in the feeding of the dairy cow

2008 ◽  
Vol 15 (3) ◽  
pp. 235 ◽  
Author(s):  
P. PURSIAINEN ◽  
M. TUORI
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Protein Concentration ◽  
Milk Protein ◽  
Latin Square ◽  
Crude Protein Content ◽  
Grass Silage ◽  
Ad Libitum ◽  
Ad Libitum Intake ◽  
Barley Silage

The effect of replacing wilted grass silage (GS) with pea-barley intercrop silage (PBS) on feed intake, diet digestibility and milk production was studied with 8 multiparous Ayrshire-cows in a replicated 4 × 4 Latin square experiment. Proportion of PBS was 0 (PBS0), 33 (PBS33), 67 (PBS67) or 100 (PBS100) % of silage dry matter (DM). The DM content was 559 and 255 g kg-1 for GS and PBS. Crude protein content was 131 and 170 g kg-1 DM, respectively. Pea-barley silage was more extensively fermented than GS with total fermentation acid content of 120 vs. 12 g kg-1 DM. Silage was fed for ad libitum intake and supplemented with on the average 13 kg concentrate per day. Silage DM intake was 9.2 (PBS0), 9.7 (PBS33), 9.0 (PBS67) and 7.1 (PBS100) kg per day (Pquadr. < 0.05). The energy corrected milk yield [30.3 (PBS0), 29.8 (PBS33), 30.3 (PBS67), 31.3 (PBS100) kg per day] was not significantly affected by the treatment. Milk protein concentration decreased linearly (P < 0.05) in response to feeding PBS. It is concluded that PBS can replace up to two thirds of wilted, moderate quality GS in the feeding of dairy cows because in this experiment pure pea-barley silage reduced silage intake.

scholarly journals Mesquite Extract as Phytogenic Additive to Improve the Nutrition of Sheep

2017 ◽  
Vol 9 (7) ◽  
pp. 164 ◽  
Author(s):  
Renato Tonhá Alves Júnior ◽  
Evaristo Jorge Oliveira de Souza ◽  
Airon Aparecido Silva de Melo ◽  
Dulciene Karla De Andrade Silva ◽  
Thaysa Rodrigues Torres ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
Ingestive Behavior ◽  
Rumen Content ◽  
Ciliated Protozoa ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Four concentrations (0, 200, 400, 600 and 800 mg extract per ml of water) of mesquite extract were used as phytogenic additive to verify the potential to increase the nutritional value of the feed, ruminal parameters (primarily propionate production) and nitrogen use efficiency, microbial protein synthesis and quantify the reduction of ciliated protozoa and characterize the ingestive behavior of sheep. Ten adult male sheep were subjected to a 5 × 5 double Latin square design. Prior to feeding, the animals received the mesquite extract. Nutrient intake was estimated from the difference of the amount of feed provided and the total surplus. Rumen content samples were collected to evaluate the profile of short-chain fatty acids, ammonia nitrogen, pH, ciliated protozoa, turnover rate and disappearance rate. To estimate the microbial protein synthesis, the technique of purine derivatives was used. The mesquite extract quadratically increased (P < 0.05) the digestibility of dry matter, organic matter, crude protein and total digestible nutrients, as well as increased propionate production, acetate:propionate ratio and microbial protein synthesis. The numbers of ciliate protozoa in the rumen decreased as a result of mesquite extract inclusion in the diet. The use of mesquite pod extract at a concentration of 488 mg/mL is recommended to improve digestibility of dry matter, organic matter, crude protein and total digestible nutrients, and to optimize microbial protein synthesis and increase propionic acid production.

The use of near infra-red reflectance spectroscopy (nirs) for the evaluation of forages. The application to undried silage

1992 ◽  
Vol 1992 ◽  
pp. 221-221
Author(s):  
C W Baker ◽  
D I Givens
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Agricultural Development ◽  
Reflectance Spectroscopy ◽  
Wet Chemistry ◽  
Advisory Service ◽  
Infra Red ◽  
Routine Basis

NIRS is now a familiar tool in the assessment of forage and feedingstuff quality. Recently NIRS has been applied to the direct prediction of in vivo organic matter digestibility (OMD) of grass silages (Barber et al 1990) and cereal straws (Givens et al 1991).The Agricultural Development and Advisory Service (ADAS) has been using NIRS to predict the in vivo OMD of oven dried silages on a routine basis since 1989, and it has proved to be reliable, accurate and rapid. In addition to OMD, crude protein (CP), and neutral detergent fibre (NDF), are routinely predicted by NIRS, leaving pH, ammonia, dry matter and ash still to be determined by wet chemistry methods.

scholarly journals PSIV-13 Supplemental phytase increases phosphorus digestibility in pigs regardless of phytase source or feed pelleting

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 229-229
Author(s):  
Bokyung Hong ◽  
Beob G Kim
Keyword(s):  
Escherichia Coli ◽  
Organic Matter ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Basal Diet ◽  
Inorganic P ◽  
Different Doses ◽  
Fecal Collection

Abstract Two experiments were conducted to investigate the effects of phytase source and feed pelleting on digestibility of dry matter (DM), crude protein, organic matter (OM), and phosphorus (P) with different doses of phytase in pigs. In Exp. 1, ten barrows (31.4 ± 3.5 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 6 Latin square design with 5 diets and 6 periods. In Exp. 2, ten barrows (28.2 ± 3.2 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 5 Latin square design with 5 diets and 5 periods. Each period lasted 10 d consisted of a 5-d adaptation and a 5-d collection periods and the marker to marker method was used for fecal collection. In both experiments, a basal diet was prepared based on corn and soybean meal without phytase or inorganic P. Four additional diets were prepared in a 2 × 2 factorial treatment arrangement with 2 phytase sources (Buttiauxella spp. or Escherichia coli) and 2 feed forms (mash or pellet). Both experiments employed the same treatments but had different supplemental phytase doses (500 and 1,500 FTU/kg in Exp. 1 and 2, respectively). In Exp. 1, supplemental phytase increased standardized total tract digestibility of P (66.1 vs. 51.7%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM, crude protein, and OM (P &lt; 0.001). In Exp. 2, supplemental phytase increased standardized total tract digestibility of P (72.8 vs. 44.8%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM and OM (P &lt; 0.001). Taken together, supplemental phytase at 500 and 1,500 FTU/kg increases P digestibility regardless of phytase source or feed pelleting.

Mixtures of annual legumes and small-grained cereals for forage production under low rainfall

1989 ◽  
Vol 113 (2) ◽  
pp. 249-253 ◽  
Author(s):  
D. N. Droushiotis
Keyword(s):  
Organic Matter ◽  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Crude Protein Content ◽  
Forage Production ◽  
Legume Seed ◽  
Matter Production ◽  
Digestible Organic Matter ◽  
Triticale Line

SUMMARYTwo small-grained cereals, Mulga oats and a triticale line, and two legumes, local vetch and local peas, were grown in pure stands and in mixtures at various cereal: legume seed ratios (20:80, 40:60, 60:40 and 80:20) for three successive cropping years, 1981/82 to 1983/84, at Laxia and Dromolaxia, Cyprus, in each year.The cereal pure stands produced, on average, more dry matter, 8·40 t/ha, and more digestible organic matter, 4·12 t/ha, than the legume pure stands, 3·68 and 2·18 t/ha, respectively. On average, total dry matter production decreased linearly as the seed proportion of the legume component in the mixture increased. The proportion of the legume in the harvested material was much lower than expected from the seed ratios. The highest proportion of legume was seen in the mixtures of peas with triticale and ranged from 8·1 to 35·5% at the various sowing rates. Digestibility and crude protein content were highest in the mixtures of triticale and peas.

UTILIZATION OF LODGEPOLE PINE (Pinus contorta) MUKA (FOLIAGE) BY SHEEP

1982 ◽  
Vol 62 (2) ◽  
pp. 467-471
Author(s):  
R. M. TAIT ◽  
C. GASTON ◽  
J. R. HUNT ◽  
G. M. BARTON
Keyword(s):  
Organic Matter ◽  
Pinus Contorta ◽  
Dry Matter ◽  
Nutritive Value ◽  
Lodgepole Pine ◽  
Latin Square ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Acid Detergent Fiber ◽  
Alfalfa Meal

Eight sheep were used in two 4 × 4 latin square digestion trials substituting alfalfa meal with unsteamed or steamed muka (foliage) at levels of 20, 40 or 60%. Including muka at all levels depressed (P < 0.05) the digestibility of dry matter, organic matter, crude protein and acid detergent fiber compared with the control alfalfa meal diet. When calculated by difference, the digestibility of muka dry matter and organic matter was not affected (P < 0.05) by either the level of its inclusion in the diets or by steaming. The mean dry matter and organic matter digestibilities of muka were 29.2 and 30.4%, respectively. The protein content of muka was 5.0% and the estimated protein digestibility at the 60% level of inclusion was only 6.6%. With unsteamed muka, the digestibility of acid detergent fiber calculated by difference averaged −11.9%, suggesting the presence of factors which depressed the digestibility of fiber in the basal diet. Steaming appeared to reduce this depression. A growth trial was conducted with lambs where alfalfa meal, as the sole roughage source, was compared to the same diet with 20% of the alfalfa replaced by steamed muka. Growth rates were not different (P > 0.05) being 142 and 138 g/day, respectively. A taste panel evaluation of the carcasses found no adverse effects on flavor due to the inclusion of steamed muka in the diet. Key words: Lodgepole pine muka, nutritive value, sheep

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