scholarly journals 3D printing of custom sample holders as a responsive and cost-effective method of sample holder generation for electron microscopy.

2021 ◽  
Vol 27 (S1) ◽  
pp. 3172-3174
Author(s):  
Alex Strachan ◽  
Dan Haspel ◽  
Natasha Stephen
2012 ◽  
Vol 15 (3) ◽  
pp. 589-596 ◽  
Author(s):  
K. Stojecki ◽  
J. Karamon ◽  
J. Sroka ◽  
T. Cencek

Abstract Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Tien-Jen Chang ◽  
Lukas Vaut ◽  
Martin Voss ◽  
Oleksii Ilchenko ◽  
Line Hagner Nielsen ◽  
...  

AbstractConventional photopolymerization-based 3D printing still requires developing a concise and cost-effective method to improve the printing resolution at the nanoscale. Here, we propose the use of a gaming console optical drive pickup unit for 3D photopolymerization. This mass-produced optical pickup unit features a finely adjustable diode laser, allowing us to adjust the printing resolution from tens of micrometres down to hundreds of nanometres without requiring oxygen radical scavenging or costly femtosecond lasers. We evaluate the 3D printing performance using a commercial photopolymer under different laser exposure parameters. The proposed printing system achieves a resolution of 385 nm along the lateral direction and XYZ nano-resolution linear stages enable a printing volume of up to 50 × 50 × 25 mm3. Finally, we demonstrate the fabrication of 3D stereoscopic microstructures. The substantially simplified optics proposed here paves the way for affordable high-resolution micro/nanoscale 3D fabrication.


2018 ◽  
Vol 25 (04) ◽  
pp. 1850087
Author(s):  
DONGDONG LIANG ◽  
SHIMIN LIU ◽  
ZHINUO WANG ◽  
YU GUO ◽  
WEIWEI JIANG ◽  
...  

The composite of a nanocrystalline SnO2 thick film deposited on an Al-doped ZnO ceramic substrate was firstly proposed. This study also provided a simple, fast and cost effective method to prepare SnO2 thick film and Al-doped ZnO ceramic as well as the final composite. The crystal structure, morphology, composition, pore size distribution and gas sensitivity of the composite were investigated by means of X-ray diffraction, scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, Barrett–Joyner–Halenda analysis and gas sensitive measurement system. Results indicated that the composite was fully porous consisted of SnO2, ZnO and ZnAl2O4 crystal phases. The macrosized pores generated in the composite could enhance the gas infiltration into the sensing layers effectively. In this way, combining a high gas-transporting-capability and a nanocrystalline SnO2 thick film, the composite showed very impressive performance. The gas sensitivity of the composite was high enough for ethanol vapor with different concentrations, which was comparable to other kinds of reported SnO2 gas sensors, while showing two straight lines with a turning point at 1000[Formula: see text]ppm. Finally, the gas sensitive mechanism was proposed based on the microstructure and composition of the composite.


2013 ◽  
Vol 11 (8) ◽  
pp. 1393-1401 ◽  
Author(s):  
Mehdi Rahimi-Nasrabadi ◽  
S. Pourmortazavi ◽  
Morteza Khalilian-Shalamzari ◽  
S. Hajimirsadeghi ◽  
M. Zahedi

AbstractA simple and fast chemical method was used for synthesis of manganese tungstate nanoplates in flower-like clusters; while Taguchi robust design was employed as statistical method for optimization of the experimental parameters for the procedure. Ultrafine manganese tungstate plates in flower-like clusters were synthesized via a direct precipitation method involving addition of manganese ion solution to the aqueous tungstate reagent. Effects of various reaction conditions such as manganese and tungstate concentrations, flow rate of reagent addition and reactor temperature on the thickness of the synthesized manganese tungstate plates were investigated experimentally. Analysis of variance (ANOVA) showed that manganese tungstate nanoplates could be effectively synthesized by tuning significant parameters of precipitation procedure. Meanwhile, optimum conditions for synthesis of MnWO4 nanoplates via this simple, fast, and cost effective method were proposed. The structure and composition of the prepared nanoplates under optimum conditions were characterized by EDX, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), FT-IR spectroscopy, and photoluminescence techniques.


Author(s):  
Lawrence M. Roth

The female reproductive tract may be the site of a wide variety of benign and malignant tumors, as well as non-neoplastic tumor-like conditions, most of which can be diagnosed by light microscopic examination including special stains and more recently immunoperoxidase techniques. Nevertheless there are situations where ultrastructural examination can contribute substantially to an accurate and specific diagnosis. It is my opinion that electron microscopy can be of greatest benefit and is most cost effective when applied in conjunction with other methodologies. Thus, I have developed an approach which has proved useful for me and may have benefit for others. In cases where it is deemed of potential value, glutaraldehyde-fixed material is obtained at the time of frozen section or otherwise at operation. Coordination with the gynecologic oncologist is required in the latter situation. This material is processed and blocked and is available if a future need arises.


The choice of cost-effective method of anticorrosive protection of steel structures is an urgent and time consuming task, considering the significant number of protection ways, differing from each other in the complex of technological, physical, chemical and economic characteristics. To reduce the complexity of solving this problem, the author proposes a computational tool that can be considered as a subsystem of computer-aided design and used at the stage of variant and detailed design of steel structures. As a criterion of the effectiveness of the anti-corrosion protection method, the cost of the protective coating during the service life is accepted. The analysis of existing methods of steel protection against corrosion is performed, the possibility of their use for the protection of the most common steel structures is established, as well as the estimated period of effective operation of the coating. The developed computational tool makes it possible to choose the best method of protection of steel structures against corrosion, taking into account the operating conditions of the protected structure and the possibility of using a protective coating.


Author(s):  
Yuliya Prozherina ◽  

3D printing of drugs is an innovative and cost-effective technology, which is a major step towards personalized medicine. This technology can be used for the development of controlled-release drugs; fixed-dose combination drugs, as well as for the creation of orodispersible dosage forms. The global 3D drug market is still largely at the research stage, but its rapid growth is expected in the coming decade [1].


1996 ◽  
Vol 33 (8) ◽  
pp. 23-29 ◽  
Author(s):  
I. Dor ◽  
N. Ben-Yosef

About one hundred and fifty wastewater reservoirs store effluents for irrigation in Israel. Effluent qualities differ according to the inflowing wastewater quality, the degree of pretreatment and the operational parameters. Certain aspects of water quality like concentration of organic matter, suspended solids and chlorophyll are significantly correlated with the water column transparency and colour. Accordingly optical images of the reservoirs obtained from the SPOT satellite demonstrate pronounced differences correlated with the water quality. The analysis of satellite multispectral images is based on a theoretical model. The model calculates, using the radiation transfer equation, the volume reflectance of the water body. Satellite images of 99 reservoirs were analyzed in the chromacity space in order to classify them according to water quality. Principal Component Analysis backed by the theoretical model increases the method sensitivity. Further elaboration of this approach will lead to the establishment of a time and cost effective method for the routine monitoring of these hypertrophic wastewater reservoirs.


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