Effect of Citric Pectin on beta-Carotene Bioavailability in Rats

2002 ◽  
Vol 72 (4) ◽  
pp. 199-203 ◽  
Author(s):  
Márcia Zanutto ◽  
Alceu Jordão Júnior ◽  
Mônica Meirelles ◽  
Rosa Fávaro ◽  
Hélio Vannucchi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Wistar Rats ◽  
Beta Carotene ◽  
Retinyl Palmitate ◽  
Control Group ◽  
Plasma Retinol ◽  
Experimental Group ◽  
Retinol Concentration

The effect of citric pectin on the bioavailability of synthetic beta-carotene was studied. Thirty Wistar rats were used, ten animals were sacrificed at the beginning of the experiment and remaining animals were divided into two groups and received the following diets for 30 days: control group (CG) – 24 mg beta-carotene/g diet + 0% citric pectin; experimental group (EG) – 24 mugbeta-carotene/g diet + 7% citric pectin. Plasma and liver beta-carotene, vitamin A, and retinyl palmitate concentrations were determined by high-performance liquid chromatography (HPLC). Plasma retinol concentration was 1.42 ± 0.36 mumol/L for CG and 1.10 ± 0.24 mumol/L for EG (p = 0.1), and plasma beta-carotene concentration was 0.20 ± 2.51 mumol/L for CG and 0.07 ± 0.04 µumol/L for EG (p = 0.01). Only traces of retinyl palmitate were detected in CG and none in EG. Retinol did not differ significantly between groups CG and EG, while a significantly higher beta-carotene concentration was observed for CG. Liver concentrations of retinol (CG: 4.90 ± 2.51 µug/g; EG: 2.68 ± 1.12 µug/g), beta-carotene (CG: 0.98 ± 0.28 µug/g; EG: 0.11 ± 0.06 µug/g), and retinyl palmitate (CG: 95.47 ± 45.13 µug/g, EG: 37.01 ± 17.20 µug/g) differed significantly between groups (p < 0.05), with a lower concentration being observed for EG. We conclude that 7% citric pectin in the rat diet decreases the bioavailability of synthetic beta-carotene, reducing the liver reserves of vitamin A and beta-carotene.

scholarly journals Analysis of Vitamin A in Multivitamin Products

2021 ◽  
Vol 11 (3) ◽  
pp. 174-184
Author(s):  
A. S. Alekseeva ◽  
T. B. Shemeryankina ◽  
M. N. Lyakina ◽  
M. S. Smirnova ◽  
E. P. Fedorova ◽  
...  
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Beta Carotene ◽  
Test Methods ◽  
Retinyl Palmitate ◽  
Test Method ◽  
Medicinal Products ◽  
Retinyl Acetate ◽  
Preliminary Purification

Vitamin A is present in multivitamin products mainly in the form of retinol esters: retinyl acetate, retinyl palmitate, and beta carotene—retinol precursor (dimer) found in plants, which is capable of converting into retinol in liver cells. Retinol is determined in medicinal products primarily by high performance liquid chromatography (HPLC), with preliminary purification and vitamin isolation by liquid-liquid extraction. However, scientific literature also describes other methods of sample preparation and analysis of such compounds. An important issue is differentiation of vitamin A from other fat-soluble vitamins often included as components in multivitamin products. The aim of the study was to analyse and summarise data on current methods used for determination of vitamin A and its derivatives in medicinal products. The authors analysed the range of vitamin A products authorised in the Russian Federation, and the test methods described in their product specification files. The study demonstrated that the test method most often used for determination of retinol esters was HPLC with isocratic elution mode using octadecylsilyl packing in the reverse-phase mode, and, less frequently, aminopropylsilyl packing in the normal phase mode. Determination of beta carotene in medicinal products is most often performed using spectrophotometry. 

scholarly journals Gestational diabetes: a risk of puerperal hypovitaminosis A?

2015 ◽  
Vol 87 (1) ◽  
pp. 463-470 ◽  
Author(s):  
FERNANDA B.S. RESENDE ◽  
LARISSA Q. DE LIRA ◽  
EVELLYN C. GRILO ◽  
MAYARA S.R. LIMA ◽  
ROBERTO DIMENSTEIN
Keyword(s):  
Gestational Diabetes ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Test Group ◽  
Control Group ◽  
Serum Retinol ◽  
Cross Sectional ◽  
The Individual ◽  
Retinol Concentration

The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference was found in retinol concentration in maternal milk and serum between the groups. However, it was observed that 16.7% had vitamin A deficiency in the group of patients with diabetes and only 4.1% had such deficiency in the control group. Although no difference was found in colostrum and serum retinol concentration between women with and without gestational diabetes, the individual analysis shows that those with diabetes are at higher risk of being vitamin A deficient.

scholarly journals Streptozotocin-induced diabetes in rats is associated with impaired metabolic availability of vitamin A (retinol)

1996 ◽  
Vol 75 (4) ◽  
pp. 615-622 ◽  
Author(s):  
P. J. Tuitoek ◽  
S. Ziari ◽  
A. T. C. Tsin ◽  
R. V. Rajotte ◽  
Miyoung Suh ◽  
...  
Keyword(s):  
Vitamin A ◽  
Wistar Rats ◽  
Plasma Concentrations ◽  
Basal Diet ◽  
Diabetic Rats ◽  
Lower Plasma ◽  
Vitamin A Supplementation ◽  
Streptozotocin Induced Diabetes ◽  
Plasma Retinol ◽  
Retinol Concentration

Using streptozotocin-induced diabetic Wistar rats, studies were carried out to examine the metabolic availability of vitamin A in the plasma, liver and the retina of the eye. Control and diabetic rats were fedad lib. on a semi-purified diet either with or without (basal) vitamin A supplementation, or pair-fed on the basal diet for 4 weeks. Despite the fact that diabeticrats consumed 48% more feed, they had lower plasma concentrations of retinol (P<0·003). The decrease in plasma retinol concentration was a response to diabetes (or diabetes-induced trauma), since neither pair-feeding (P<0·01) nor vitamin A supplementation altered this effect (P<0·05). Furthermore, the hepatic concentrations of the vitamin in these animals remained elevated and this increase was greater in the supplemented diabetic group (P<0·001). Decreases in 11-cis retinal (a component of rhodopsin) concentrations in the retina were also observed in diabetic animals. The increased hepatic and the decreased plasma and retina vitamin Alevels suggest a defect in the transport of the vitamin from the liver.

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kela

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
LILY RESTUSARI ◽  
SRI WIDIA NINGSIH ◽  
YULIANA ARSIL
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kelangsungan hidup anak dan standar upaya penyelamatan proses kehamilan dan persalinan. Namun pemanasan yang terjadi selama proses penggorenganpada minyak dapatmerusak jumlah vitamin A yang difortifikasi. Tujuan penelitian ini untuk membandingkan nilai retensi vitamin A di dalam kentang yang digoreng dengan menggunakan minyak goreng curah dan minyak goreng kemasan fortifikasi dengan pengaruh suhu dan waktu penggorengan. Penelitian ini menggunakan metode eksperimental disain rancangan acak lengkap (RAL) dengan dua faktor, suhu (140dan 170oC) dan waktu penggorengan (10 dan 15 menit) tanpa pengulangan.Sampel penelitian yang digunakan adalah kentang beku, minyak goreng curah dan minyak goreng kemasan fortifikasi.Nilai retensi vitamin A kentang dan minyak goreng diukur dengan menggunakan metode High Performance Liquid Chromatography (HPLC).Analisis perbedaan dan interaksi variabel diolah dengan ANOVA dua arah. Berdasarkan luas area puncak vitamin A pada kromatogram sebelum dan setelah penggorengan, ditemukan kecenderungan penurunan persentase retensi vitamin A dari minyak goreng kemasan fortifikasi dibandingkan minyak goreng curah.Nilai retensi vitamin A pada kentang yang digoreng dengan minyak goreng curah adalah 217-251%,sedangkan retensivitamin A kentang yang digoreng dengan minyak goreng kemasan fortifikasi adalah 48-131%. Analisis ANOVA dua arah tanpa pengulangan untuk persen retensi vitamin A menunjukkan bahwa (p<0,05) variabel jenis minyak goreng berpengaruh secara nyata terhadap retensi vitamin A. Retensi vitamin A pada kentang goreng yang digoreng dengan minyak goreng curah jauh lebih tinggi dibanding minyak goreng kemasan fortifikasi

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