Einfluß von Ramipril auf den Verlauf des Plasma-Thrombomodulinspiegels bei Diabetikern

VASA ◽  
1999 ◽  
Vol 28 (3) ◽  
pp. 172-180 ◽  
Author(s):  
Borcea ◽  
Morcos ◽  
Isermann ◽  
Henkels ◽  
Ziegler ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Cell ◽  
Enzyme Inhibitor ◽  
Cell Damage ◽  
Treated Group ◽  
Control Group ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Double Blind Study ◽  
Endothelial Cell Damage

Background: In diabetic patients endothelial dysfunction is reflected by an increased urinary albumine excretion, which can be reduced by ACE-inhibitors. No data are available showing a endothelial-protective effect by determining a marker reflecting endothelial cell-damage. Patients and methods: The effect of angiotensin converting enzyme inhibitor (ACEI) (ramipril) treatment on the progression of endothelial cell damage, – assessed by measurement of plasma-thrombomodulin (TM), – was investigated in an open, non randomized, prospective pilot study over a period of 18 months in diabetic patients. 87 patients with an urinary albumin concentration (UAC) below 100 mg/l at baseline were included. 46 patients were treated without ACEI and served as a control group, 41 patients were treated with ACEI. Participation in this study did not affect intensity in the treatment of blood glucose, blood pressure or diet. At study entry both groups were comparable with respect to duration of diabetes, diabetic complications, vascular risk factors, body mass index, medications used to treat diabetes, presence of hypertension, glycemic control, tryglycerides, HDL cholesterol, creatinine, UAC and plasma-TM. Age, blood pressure, and total cholesterol were significantly higher in the ACEI group, compared with the control group. Results: After a follow up of 18 months a significant increase in UAC (DeltaUAC = 10.48 mg/l, p = 0.03) and plasma-TM (DeltaTM = 3.06 ng/l, p = 0.009) was observed in the control group, while in the ACEI treated group a decrease in albuminuria (DeltaUAC = –7.44 mg/l, p = 0.01) and plasma-TM (DeltaTM = –4.78 ng/l, p = 0.001) was seen. Despite a similar approach in hypertension and diabetes control in both groups, UAC and plasma-TM decreased after 18 months only in the ACEI treated group. Treatment with ACEI was the strongest predictor (p=0.0001) indicating decrease of UAC and plasma-TM (multi regression analysis). Conclusion: Plasma-thrombomodulin might be a useful marker for assessing the efficacy of drugs potentially protecting the vessel wall. While the present study was a open, non randomized study, further investigation is necessary to proof the hypothesis in a randomized, placebo-controlled, double-blind study.

Kinetics of Thrombin Generation in Patients with Multiple Myeloma.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3535-3535 ◽  
Author(s):  
Anna D. Petropoulou ◽  
Grigoris T. Gerotziafas ◽  
Kostas Zervas ◽  
A. Mpanti ◽  
Michel Meyer Samama ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Endothelial Cell ◽  
Plasma Levels ◽  
Thrombin Generation ◽  
Cell Damage ◽  
Control Group ◽  
Endothelial Cell Damage ◽  
Thrombotic Risk ◽  
Soluble Thrombomodulin ◽  
Significant Difference

Abstract Thalidomide has emerged as a promising treatment for multiple myeloma (MM). Thrombosis is the most serious complication of thalidomide therapy, essentially when it is combined with dexamethasone. The pathogenesis of thrombosis in MM patients (pts) treated with thalidomide is not clear and probably of multi factorial origin. We used the Thrombin Generation test (TGT) and measured the plasma levels of soluble thrombomodulin (sTM) to better clarify the MM-related and thalidomide-related thrombogenicity. TGT was performed in citrated frozen platelet poor plasma (PPP). Blood was obtained from 26 MM pts, Salmon and Durie stage II and III, 62.5 years old (42–77), 9 males and 17 females, 10 treated with thalidomide (100–200mg/d orally) and dexamethasone (40mg/d for 4 days) (TD group) and 16 receiving no treatment (MM group). 13 healthy volunteers formed the control group. Thrombin Generation (TG) was initiated by adding the PPP reagent (Thrombogram-Thrombinoscope®) and the triggering solution (CaCl2 and fluorogenic substrate). We analyzed the endogenous thrombin potential (ETP), the Cmax and the velocity index of TG. The plasma levels of sTM in PPP were measured by a specific ELISA (Diagnostica Stago, France). In the MM group we observed an increase of the ETP, though not significant compared to the controls. The Cmax was almost equal to the control group value, while the velocity index of TG was statistically lower in the MM group compared to controls. In the TD group, a statistically significant increase of ETP was observed as compared to the control group. The Cmax was higher, compared to controls, though not significantly, whereas the velocity index of TG was almost equal to the control group value. There was no significant difference in the TG parameters between MM and TD groups. sTM in the control group was 45±14ng/ml. Both groups of pts had significantly increased sTM plasma levels as compared to the control but the difference between the two groups did not reach significance. Results are shown in Table 1. In patients with MM coexists an increase of sTM, a marker of endothelial cell damage, together with an increased TG capacity. The addition of thalidomide treatment is associated with a slight but not significant increase of ETP and Cmax. The co-existence of endothelial cell damage with increased TG capacity could be associated to the increased thrombotic risk in MM patients treated with thalidomide. This hypothesis will be controlled in a prospective study. Table 1: Thrombogram parameters and sTM plasma levels of studied pts. Control MM group TD group * Results significantly different between the MM and TD groups and the control group (p<0.05 vs the control group) ETP (nM×min) 1399±297 1651±478 1747±448* Cmax (nM) 366±54 342±52 402±99 Velocity Index (nM/min) 198±45 160±19* 184±65 STM (ng/ml) 45±14 84±42* 73±30*

scholarly journals Assessment of Corneal Endothelium among Diabetic Patients in a Multispecialty Hospital in Tamil Nadu

2020 ◽  
Author(s):  
Soundaram Meenakshisundaram ◽  
Melina I Sahay ◽  
Damal Kandadai Sriram ◽  
Melvin George
Keyword(s):  
Blood Pressure ◽  
Endothelial Cell ◽  
Blood Sugar ◽  
Cell Density ◽  
Corneal Thickness ◽  
Diabetic Group ◽  
Control Group ◽  
Diabetic Patients ◽  
Duration Of Diabetes ◽  
Significant Difference

Introduction: Corneal damage is the common issue affecting 70% of diabetic patients. Diabetic cornea has functional abnormalities such as decreased corneal Endothelial Cell Density (ECD), thicker corneas with increased endothelial permeability, lesser corneal sensitivity, and elevated corneal auto-fluorescence. Aim: To study the corneal endothelial characteristics among diabetic patients and to identify the factors associated with endothelial damage and compare the corneal changes based on the duration of diabetes mellitus and glycaemic control. Materials and Methods: The hospital based cross-sectional study was conducted in 155 patients (100 diabetic and 55 control) from 24th September 2016 to 29th March 2018. The corneal endothelial morphological features were evaluated using a non-contact EM-3000 specular microscope. The morphological characteristics of endothelial cell like the ECD, Coefficient of Variation (CV), Central Corneal Thickness (CCT), and percentage of hexagonal cells were compared between diabetic patients and the control population. Age, gender, blood pressure, duration of diabetes, fasting and Postprandial Blood Sugar (PPBS) HbA1c value, blood urea, and serum creatinine, Intra Ocular Pressure (IOP) were also recorded, and the endothelial cell characteristics were compared between the diabetic groups according to the duration (< or ≥ 3 years) and HbA1c% (< or ≥ 7%). An independent t-test was performed to compare the means of endothelial characteristics between study subjects and the control group and also between duration of diabetes and HbA1C levels. A p-value of less than 0.05 was considered statistically significant. The data were analysed using a SPSS statistical program. Results: Baseline characteristics like Fasting Blood Sugar (FBS), PPBS, HbA1c, Systolic and Diastolic Blood Pressure (SBP and DBP) were significantly higher in diabetic group than control. A significant difference in corneal thickness (p=0.01) and hexagonality (p<0.001) were also observed between both the groups. The diabetic group showed a reduction in cell density and a higher coefficient variation of cell size however the differences were not statistically significant, There was no significant difference in the ECD, CCT, CV, and hexagonality irrespective to HbA1c level and duration of diabetes. Conclusion: Patients with diabetes had a significant increase in corneal thickness, and a higher frequency of pleomorphism compared to control subjects. In terms of ECD and co-efficient variation, no difference was observed between groups.

scholarly journals The Tale of Two Challanges: Stem Cells and Diabetic Vasculopathy

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
Y M Naguib ◽  
S M Abdelghany ◽  
R I Noreldin
Keyword(s):  
Blood Pressure ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Complications ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Diabetic Patients ◽  
Human Umbilical Cord ◽  
Systemic Complications

Abstract Background Diabetes is a metabolic disorder highly linked to several systemic complications. Diabetic patients largely suffer from hyperglycemia-induced macro- and micro-vascular abnormalities. Accumulating data have suggested a beneficial role of endothelial progenitor cells in diabetic microvascular diseases. Objective We evaluated the possible therapeutic effect of injecting transformed human umbilical cord mesenchymal stem cells on cardiovascular and renal functions in old diabetic rats. Methods Thirty old (18-14 months) male Wistar albino rats weighing 300-350g were used in the present study. Diabetes was induced by intra-peritoneal streptozotocin injection. Rats were assigned (10/group) to Naive (received no treatment), diabetic control (injected with saline), and diabetic transformed mesenchymal stem cell treated (TMSCs). Measurement of blood pressure and doppler studies were performed, and blood samples were collected. Animals were then scarified and large and small vessels were collected for immunohistopathology. Results Anti-CD31 immuno-staining has shown successful homing of the injected transformed stem cells to the vascular endothelium. TMSCs treated group featured reduced systolic blood pressure, heart rate and pulse wave velocity when compared to control group. TMSCs treated group had lower serum level of vascular endothelial growth factor (VEGF), interleukin1β (IL-1β) and tumor necrosis factor alpha (TNFα). Renal function parameters (KIM-1 and cystatin C)) were significantly lower in TMSCs treated group. Renal artery doppler study revealed improved blood flow and reduced resistance in the TMSCs treated group when compared to the control group. Conclusion We show here that transformed mesenchymal stem cells could be a potential therapeutic approach against hyperglycemia-induced macro- and micro-vascular complications in aged diabetics.

scholarly journals Endothelial cell damage is the central part of COVID-19 and a mouse model induced by injection of the S1 subunit of the spike protein

2021 ◽  
Vol 51 ◽  
pp. 151682
Author(s):  
Gerard J. Nuovo ◽  
Cynthia Magro ◽  
Toni Shaffer ◽  
Hamdy Awad ◽  
David Suster ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Mouse Model ◽  
Cell Damage ◽  
Spike Protein ◽  
Endothelial Cell Damage

Antioxidant activities of aqueous leaf extracts of Toona sinensis on free radical-induced endothelial cell damage

2011 ◽  
Vol 137 (1) ◽  
pp. 669-680 ◽  
Author(s):  
Hsin-Ling Yang ◽  
Ssu-Ching Chen ◽  
Kai-Yuan Lin ◽  
Mei-Tsun Wang ◽  
Yu-Chang Chen ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Cell Damage ◽  
Endothelial Cell Damage ◽  
Leaf Extracts ◽  
Toona Sinensis

Effect of hyperglycemia on brain pH levels in areas of focal incomplete cerebral ischemia in monkeys

1986 ◽  
Vol 65 (5) ◽  
pp. 693-696 ◽  
Author(s):  
W. Richard Marsh ◽  
Robert E. Anderson ◽  
Thoralf M. Sundt
Keyword(s):  
Adverse Effect ◽  
Cerebral Ischemia ◽  
Cell Damage ◽  
Treated Group ◽  
Squirrel Monkeys ◽  
Control Group ◽  
Content Type ◽  
Brain Ph ◽  
Fine Print ◽  
Incomplete Ischemia

✓ The adverse effect of a minimal cerebral blood flow (CBF) in models of global ischemia has been noted by many investigators. One factor believed important in this situation is the level of blood glucose, since a continued supply of this metabolite results in increased tissue lactate, decreased brain pH, and increased cell damage. The authors have extended these observations to a model of focal incomplete ischemia. Brain pH was measured in fasted squirrel monkeys in regions of focal incomplete ischemia after transorbital occlusion of the middle cerebral artery (MCA). In both control and hyperglycemic animals, CBF was reduced to less than 30% of baseline. At 3 hours after MCA occlusion, brain pH in the control group was 6.66 ± 0.68 as compared to 6.27 ± 0.26 in the glucose-treated group. This difference was statistically significant by Student's unpaired t-test (p < 0.05). Thus, hyperglycemia results in decreased tissue pH in regions of focal incomplete cerebral ischemia in monkeys.

scholarly journals Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma

2013 ◽  
Vol 44 (1) ◽  
pp. 6 ◽  
Author(s):  
Albina Sokoli ◽  
Katrin Groebel ◽  
Katharina Hoelzle ◽  
Werner M Amselgruber ◽  
José M Mateos ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Damage ◽  
Cell Tropism ◽  
Endothelial Cell Damage ◽  
Mycoplasma Suis ◽  
Hemotrophic Mycoplasma ◽  
Insight Into

ASSOCIATION BETWEEN ANTERIOR CHAMBER DEPTH AND ENDOTHELIAL CELL LOSS AFTER PHACOEMULSIFICATION

2021 ◽  
pp. 30-31
Author(s):  
Gargi Verma ◽  
Kishor Kumar
Keyword(s):  
Endothelial Cell ◽  
Anterior Chamber ◽  
Cell Damage ◽  
Anterior Chamber Depth ◽  
Cell Loss ◽  
Endothelial Cell Loss ◽  
Endothelial Cell Damage ◽  
Percentage Decrease ◽  
Central Cornea ◽  
Shallow Anterior Chamber

Purpose: To assess the association between anterior chamber depth and endothelial cell loss after phacoemulsication Material and Methods: Ninety patients were recruited for this comparative type of observational study who were further divided into 3 groups according to anterior chamber depth (ACD). All patients underwent phacoemulsication procedure and intraocular implantation. Postoperatively endothelial cell loss (ECL) was calculated by measuring percentage decrease in endothelial cell density of central cornea and regression coefcient between ACD and ECLwas assessed in each group. Results: Endothelial cell loss was signicantly higher in shallow anterior chambers to other groups and the association between ACD and ECLwas signicant only in shallow anterior chamber depth group. Conclusion: Eyes with shallow anterior chamber depth are at higher risk for endothelial cell damage and shows an association between ACD and ECLwhen compared to deep anterior chamber depth.

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