Effects of aneuploidy on cell behaviour and function

2022 ◽  
Author(s):  
Rong Li ◽  
Jin Zhu
Keyword(s):  
Cell Behaviour ◽  
And Function

scholarly journals The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function, which leads to early-onset osteoarthritis

2018 ◽  
Vol 373 (1759) ◽  
pp. 20170335 ◽  
Author(s):  
Elizabeth A. Lawrence ◽  
Erika Kague ◽  
Jessye A. Aggleton ◽  
Robert L. Harniman ◽  
Karen A. Roddy ◽  
...  
Keyword(s):  
Early Onset ◽  
Mechanical Performance ◽  
Bone Development ◽  
Type Ii Collagen ◽  
Cell Behaviour ◽  
Major Structural Component ◽  
Genes Encoding ◽  
Type Xi Collagen ◽  
And Function ◽  
The Impact

Collagen is the major structural component of cartilage, and mutations in the genes encoding type XI collagen are associated with severe skeletal dysplasias (fibrochondrogenesis and Stickler syndrome) and early-onset osteoarthritis (OA). The impact of the lack of type XI collagen on cell behaviour and mechanical performance during skeleton development is unknown. We studied a zebrafish mutant for col11a2 and evaluated cartilage, bone development and mechanical properties to address this. We show that in col11a2 mutants, type II collagen is made but is prematurely degraded in maturing cartilage and ectopically expressed in the joint. These changes are correlated with increased stiffness of both bone and cartilage; quantified using atomic force microscopy. In the mutants, the skeletal rudiment terminal region in the jaw joint is broader and the interzone smaller. These differences in shape and material properties impact on joint function and mechanical performance, which we modelled using finite element analyses. Finally, we show that col11a2 heterozygous carriers reach adulthood but show signs of severe early-onset OA. Taken together, our data demonstrate a key role for type XI collagen in maintaining the properties of cartilage matrix; which when lost leads to alterations to cell behaviour that give rise to joint pathologies. This article is part of the Theo Murphy meeting issue ‘Mechanics of development’.

scholarly journals The extracellular matrix: a new dimension in disease diagnosis and treatment

2016 ◽  
Vol 38 (4) ◽  
pp. 10
Author(s):  
Susan Aungier ◽  
Kim Midwood
Keyword(s):  
Extracellular Matrix ◽  
Disease Diagnosis ◽  
Structure And Function ◽  
Diagnosis And Treatment ◽  
Tissue Structure ◽  
Cell Behaviour ◽  
3D Environment ◽  
Wide Range ◽  
And Function ◽  
Function Matrix

The extracellular matrix (ECM) forms the complex and dynamic 3D environment that defines tissue structure and function. Matrix molecules provide much of the microenvironmental plasticity that dictates cell behaviour, and their dysregulated expression is associated with a wide range of diseases including cancers, and inflammatory and fibrotic conditions. Here, we describe how these matrix molecules are beginning to be used for disease diagnosis and treatment.

scholarly journals The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function, which leads to early onset osteoarthritis

2018 ◽  
Author(s):  
Elizabeth A Lawrence ◽  
Erika Kague ◽  
Jessye A Aggleton ◽  
Robert L Harniman ◽  
Karen A Roddy ◽  
...  
Keyword(s):  
Early Onset ◽  
Mechanical Performance ◽  
Bone Development ◽  
Type Ii Collagen ◽  
Cell Behaviour ◽  
Major Structural Component ◽  
Genes Encoding ◽  
Type Xi Collagen ◽  
And Function ◽  
The Impact

Abstract (max 200 words)Collagen is the major structural component of cartilage and mutations in the genes encoding Type XI collagen are associated with severe skeletal dysplasias (Fibrochondrogenesis and Stickler syndrome) and early onset osteoarthritis. The impact of the lack of Type XI collagen on cell behaviour and mechanical performance during skeleton development is unknown. We studied a zebrafish mutant for col11a2 and evaluated cartilage, bone development and mechanical properties to address this. We show that in col11a2 mutants Type II collagen is made but is prematurely degraded in maturing cartilage and ectopically expressed in the joint. These changes are correlated with increased stiffness of both bone and cartilage; quantified using Atomic Force Microscopy. In the mutants, the skeletal rudiment terminal region in the jaw joint are broader and the interzone smaller. These differences in shape and material properties impact on joint function and mechanical performance, which we modelled using Finite Element Analyses. Finally, we show that col11a2 heterozygous carriers reach adulthood but show signs of severe early onset osteoarthritis. Taken together our data demonstrate a key role for Type XI collagen in maintaining the properties of cartilage matrix; which when lost leads to alterations to cell behaviour that give rise to joint pathologies.

scholarly journals Tissue architecture: the ultimate regulator of epithelial function?

1998 ◽  
Vol 353 (1370) ◽  
pp. 857-870 ◽  
Author(s):  
Carmen Hagios ◽  
André Lochter ◽  
Mina J. Bissell
Keyword(s):  
Cell Adhesion ◽  
Cell Shape ◽  
Tissue Structure ◽  
Tissue Architecture ◽  
Cell Behaviour ◽  
Matrix Interactions ◽  
Adhesive Interactions ◽  
And Function ◽  
Extracellular Matrix Interactions

The architecture of a tissue is defined by the nature and the integrity of its cellular and extracellular compartments, and is based on proper adhesive cell–cell and cell–extracellular matrix interactions. Cadherins and integrins are major adhesion–mediators that assemble epithelial cells together laterally and attach them basally to a subepithelial basement membrane, respectively. Because cell adhesion complexes are linked to the cytoskeleton and to the cellular signalling pathways, they represent checkpoints for regulation of cell shape and gene expression and thus are instructive for cell behaviour and function. This organization allows a reciprocal flow of mechanical and biochemical information between the cell and its microenvironment, and necessitates that cells actively maintain a state of homeostasis within a given tissue context. The loss of the ability of tumour cells to establish correct adhesive interactions with their microenvironment results in disruption of tissue architecture with often fatal consequences for the host organism. This review discusses the role of cell adhesion in the maintenance of tissue structure and analyses how tissue structure regulates epithelial function.

scholarly journals Cellular sensing of micron-scale curvature: a frontier in understanding the microenvironment

Open Biology ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 190155 ◽  
Author(s):  
Richard K. Assoian ◽  
Nathan D. Bade ◽  
Caroline V. Cameron ◽  
Kathleen J. Stebe
Keyword(s):  
Molecular Mechanisms ◽  
Radius Of Curvature ◽  
Valuable Insight ◽  
Glass Plastic ◽  
Cell Behaviour ◽  
Flat Surfaces ◽  
Biological Studies ◽  
And Function ◽  
Insight Into

The vast majority of cell biological studies examine function and molecular mechanisms using cells on flat surfaces: glass, plastic and more recently elastomeric polymers. While these studies have provided a wealth of valuable insight, they fail to consider that most biologically occurring surfaces are curved, with a radius of curvature roughly corresponding to the length scale of cells themselves. Here, we review recent studies showing that cells detect and respond to these curvature cues by adjusting and re-orienting their cell bodies, actin fibres and nuclei as well as by changing their transcriptional programme. Modelling substratum curvature has the potential to provide fundamental new insight into cell behaviour and function in vivo .

Origin and function of fluctuations in cell behaviour and the emergence of patterns

2009 ◽  
Vol 20 (7) ◽  
pp. 877-884 ◽  
Author(s):  
Ana M. Mateus ◽  
Nicole Gorfinkiel ◽  
Alfonso Martinez Arias
Keyword(s):  
Cell Behaviour ◽  
And Function

scholarly journals The multifarious regulation of the apical junctional complex

Open Biology ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 190278 ◽  
Author(s):  
Alexandra D. Rusu ◽  
Marios Georgiou
Keyword(s):  
Cell Adhesion ◽  
Cell Junctions ◽  
Junctional Complex ◽  
Tissue Architecture ◽  
Comprehensive Overview ◽  
Cell Behaviour ◽  
Apical Junctional Complex ◽  
Mediate Cell ◽  
And Function ◽  
Cell Cell

Epithelial cells form highly organized polarized sheets with characteristic cell morphologies and tissue architecture. Cell–cell adhesion and intercellular communication are prerequisites of such cohesive sheets of cells, and cell connectivity is mediated through several junctional assemblies, namely desmosomes, adherens, tight and gap junctions. These cell–cell junctions form signalling hubs that not only mediate cell–cell adhesion but impact on multiple aspects of cell behaviour, helping to coordinate epithelial cell shape, polarity and function. This review will focus on the tight and adherens junctions, constituents of the apical junctional complex, and aims to provide a comprehensive overview of the complex signalling that underlies junction assembly, integrity and plasticity.

Conformation of Ribosomes from Escherichia Coli

1974 ◽  
Vol 32 ◽  
pp. 210-211
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Binding Sites ◽  
Ribosomal Proteins ◽  
Fluorescent Dyes ◽  
Protein Biosynthesis ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Dimensional Structure ◽  
Complete Understanding ◽  
And Function

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.

The Effect of Oxidized Cholesterol on the Aorta of Rabbits- Scanning Electron Microscopic Observations

1982 ◽  
Vol 40 ◽  
pp. 340-341
Author(s):  
S. K. Pena ◽  
C. B. Taylor ◽  
J. Hill ◽  
J. Safarik
Keyword(s):  
Cholesterol Biosynthesis ◽  
Cholesterol Content ◽  
Arterial Injury ◽  
Electron Microscopic ◽  
Aortic Smooth Muscle ◽  
Oxidized Cholesterol ◽  
Initial Event ◽  
Membrane Structure And Function ◽  
Scanning Electron Microscopic ◽  
And Function

Introduction: Oxidized cholesterol derivatives have been demonstrated in various cell cultures to be very potent inhibitors of 3-hvdroxy-3- methylglutaryl Coenzyme A reductase which is a principle regulator of cholesterol biosynthesis in the cell. The cholesterol content in the cells exposed to oxidized cholesterol was found to be markedly decreased. In aortic smooth muscle cells, the potency of this effect was closely related to the cytotoxicity of each derivative. Furthermore, due to the similarity of their molecular structure to that of cholesterol, these oxidized cholesterol derivatives might insert themselves into the cell membrane, alter membrane structure and function and eventually cause cell death. Arterial injury has been shown to be the initial event of atherosclerosis.

Ultrastructure of developing visual cortical synapses in the cat superior colliculus

1991 ◽  
Vol 49 ◽  
pp. 156-157
Author(s):  
Caroline A. Miller ◽  
Laura L. Bruce
Keyword(s):  
Superior Colliculus ◽  
Phosphate Buffer ◽  
Receptive Fields ◽  
Visual Cortical Area ◽  
Cortical Synapses ◽  
Visual Cortical ◽  
And Function ◽  
Phosphate Buffered Saline ◽  
The Brain ◽  
Cat Superior Colliculus

The first visual cortical axons arrive in the cat superior colliculus by the time of birth. Adultlike receptive fields develop slowly over several weeks following birth. The developing cortical axons go through a sequence of changes before acquiring their adultlike morphology and function. To determine how these axons interact with neurons in the colliculus, cortico-collicular axons were labeled with biocytin (an anterograde neuronal tracer) and studied with electron microscopy.Deeply anesthetized animals received 200-500 nl injections of biocytin (Sigma; 5% in phosphate buffer) in the lateral suprasylvian visual cortical area. After a 24 hr survival time, the animals were deeply anesthetized and perfused with 0.9% phosphate buffered saline followed by fixation with a solution of 1.25% glutaraldehyde and 1.0% paraformaldehyde in 0.1M phosphate buffer. The brain was sectioned transversely on a vibratome at 50 μm. The tissue was processed immediately to visualize the biocytin.

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