scholarly journals Deciphering the microbiome shift during fermentation of medicinal plants

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Martina Köberl ◽  
Sabine Erschen ◽  
Mohammad Etemadi ◽  
Richard Allen White ◽  
Tarek F. El-Arabi ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Time Course ◽  
Human Microbiome ◽  
Fermented Food ◽  
Gene Profiling ◽  
Rrna Gene ◽  
Positive Health ◽  
Leaf Surfaces ◽  
Natural Leaf ◽  
Positive Health Outcomes

Abstract The importance of the human-microbiome relationship for positive health outcomes has become more apparent over the last decade. Influencing the gut microbiome via modification of diet represents a possibility of maintaining a healthy gut flora. Fermented food and lactic acid bacteria (LAB) display a preventive way to inhibit microbial dysbioses and diseases, but their ecology on plants is poorly understood. We characterized the microbiome of medicinal plants (Matricaria chamomilla L. and Calendula officinalis L.) using 16S rRNA gene profiling from leaves that were fermented over a six-week time course. The unfermented samples were characterized by a distinct phyllosphere microbiome, while the endosphere revealed a high similarity. During fermentation, significant microbial shifts were observed, whereby LAB were enhanced in all approaches but never numerically dominated. Among the LAB, Enterococcaceae were identified as the most dominant family in both plants. M. chamomilla community had higher relative abundances of Lactobacillaceae and Carnobacteriaceae, while C. officinalis showed a higher presence of Leuconostocaceae and Streptococcaceae. The natural leaf microbiome and the indigenous LAB communities of field-grown Asteraceae medicinal plants are plant-specific and habitat-specific and are subjected to significant shifts during fermentation. Leaf surfaces as well as leaf endospheres were identified as sources for biopreservative LAB.

scholarly journals The power-trust cycle in global health: Trust as belonging in relations of dependency

2021 ◽  
pp. 1-21
Author(s):  
Emma-Louise Anderson ◽  
Laura Considine ◽  
Amy S. Patterson
Keyword(s):  
Global Health ◽  
Health Outcomes ◽  
Positive Health ◽  
Global Health Initiatives ◽  
Health Initiatives ◽  
Power Trust ◽  
Trust And Power ◽  
Positive Health Outcomes ◽  
Exercise Of Discretion ◽  
The Relationship

Abstract Trust between actors is vital to delivering positive health outcomes, while relationships of power determine health agendas, whose voices are heard and who benefits from global health initiatives. However, the relationship between trust and power has been neglected in the literatures on both international politics and global health. We examine this relationship through a study of relations between faith based organisations (FBO) and donors in Malawi and Zambia, drawing on 66 key informant interviews with actors central to delivering health care. From these two cases we develop an understanding of ‘trust as belonging’, which we define as the exercise of discretion accompanied by the expression of shared identities. Trust as belonging interacts with power in what we term the ‘power-trust cycle’, in which various forms of power undergird trust, and trust augments these forms of power. The power-trust cycle has a critical bearing on global health outcomes, affecting the space within which both local and international actors jockey to influence the ideologies that underpin global health, and the distribution of crucial resources. We illustrate how the power-trust cycle can work in both positive and negative ways to affect possible cooperation, with significant implications for collective responses to global health challenges.

scholarly journals Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhen Han ◽  
Peter S. Thuy-Boun ◽  
Wayne Pfeiffer ◽  
Vincent F. Vartabedian ◽  
Ali Torkamani ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Human Microbiome ◽  
Host Immunity ◽  
Rrna Gene ◽  
Metabolic Labeling ◽  
Selective Labeling ◽  
Fecal Microbiome ◽  
Acetylneuraminic Acid ◽  
Intestinal Mucus ◽  
Microbial Systems

AbstractN-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures.

When Religion Hurts: Structural Sexism and Health in Religious Congregations

2021 ◽  
pp. 000312242199668
Author(s):  
Patricia Homan ◽  
Amy Burdette
Keyword(s):  
General Social Survey ◽  
State Level ◽  
Religious Participation ◽  
Religious Institutions ◽  
Religious Congregations ◽  
Positive Health ◽  
Nationally Representative ◽  
Positive Health Outcomes ◽  
High Degree ◽  
The Relationship

An emerging line of research has begun to document the relationship between structural sexism and health. This work shows that structural sexism—defined as systematic gender inequality in power and resources—within U.S. state-level institutions and within marriages can shape individuals’ physical health. In the present study, we use a novel dataset created by linking two nationally representative surveys (the General Social Survey and the National Congregations Study) to explore the health consequences of structural sexism within another setting: religious institutions. Although religious participation is generally associated with positive health outcomes, many religious institutions create and reinforce a high degree of structural sexism, which is harmful for health. Prior research has not reconciled these seemingly conflicting patterns. We find that among religious participants, women who attend sexist religious institutions report significantly worse self-rated health than do those who attend more inclusive congregations. Furthermore, only women who attend inclusive religious institutions exhibit a health advantage relative to non-participants. We observe marginal to no statistically significant effects among men. Our results suggest the health benefits of religious participation do not extend to groups that are systematically excluded from power and status within their religious institutions.

scholarly journals Meta-Apo improves accuracy of 16S-amplicon-based prediction of microbiome function

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Gongchao Jing ◽  
Yufeng Zhang ◽  
Wenzhi Cui ◽  
Lu Liu ◽  
Jian Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
Large Scale ◽  
Low Cost ◽  
Human Microbiome ◽  
Amplicon Sequencing ◽  
Training Sample ◽  
Rrna Gene ◽  
16S Amplicon Sequencing ◽  
Cross Platform ◽  
Functional Profiles

Abstract Background Due to their much lower costs in experiment and computation than metagenomic whole-genome sequencing (WGS), 16S rRNA gene amplicons have been widely used for predicting the functional profiles of microbiome, via software tools such as PICRUSt 2. However, due to the potential PCR bias and gene profile variation among phylogenetically related genomes, functional profiles predicted from 16S amplicons may deviate from WGS-derived ones, resulting in misleading results. Results Here we present Meta-Apo, which greatly reduces or even eliminates such deviation, thus deduces much more consistent diversity patterns between the two approaches. Tests of Meta-Apo on > 5000 16S-rRNA amplicon human microbiome samples from 4 body sites showed the deviation between the two strategies is significantly reduced by using only 15 WGS-amplicon training sample pairs. Moreover, Meta-Apo enables cross-platform functional comparison between WGS and amplicon samples, thus greatly improve 16S-based microbiome diagnosis, e.g. accuracy of gingivitis diagnosis via 16S-derived functional profiles was elevated from 65 to 95% by WGS-based classification. Therefore, with the low cost of 16S-amplicon sequencing, Meta-Apo can produce a reliable, high-resolution view of microbiome function equivalent to that offered by shotgun WGS. Conclusions This suggests that large-scale, function-oriented microbiome sequencing projects can probably benefit from the lower cost of 16S-amplicon strategy, without sacrificing the precision in functional reconstruction that otherwise requires WGS. An optimized C++ implementation of Meta-Apo is available on GitHub (https://github.com/qibebt-bioinfo/meta-apo) under a GNU GPL license. It takes the functional profiles of a few paired WGS:16S-amplicon samples as training, and outputs the calibrated functional profiles for the much larger number of 16S-amplicon samples.

scholarly journals Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniel R. Leadbeater ◽  
Nicola C. Oates ◽  
Joseph P. Bennett ◽  
Yi Li ◽  
Adam A. Dowle ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Salt Marshes ◽  
Molecular Mechanisms ◽  
Surface Sediments ◽  
Gene Profiling ◽  
Oxidative Enzymes ◽  
Lignocellulose Degradation ◽  
Rrna Gene ◽  
Enzymatic Mechanisms

Abstract Background Salt marshes are major natural repositories of sequestered organic carbon with high burial rates of organic matter, produced by highly productive native flora. Accumulated carbon predominantly exists as lignocellulose which is metabolised by communities of functionally diverse microbes. However, the organisms that orchestrate this process and the enzymatic mechanisms employed that regulate the accumulation, composition and permanence of this carbon stock are not yet known. We applied meta-exo-proteome proteomics and 16S rRNA gene profiling to study lignocellulose decomposition in situ within the surface level sediments of a natural established UK salt marsh. Results Our studies revealed a community dominated by Gammaproteobacteria, Bacteroidetes and Deltaproteobacteria that drive lignocellulose degradation in the salt marsh. We identify 42 families of lignocellulolytic bacteria of which the most active secretors of carbohydrate-active enzymes were observed to be Prolixibacteracea, Flavobacteriaceae, Cellvibrionaceae, Saccharospirillaceae, Alteromonadaceae, Vibrionaceae and Cytophagaceae. These families secreted lignocellulose-active glycoside hydrolase (GH) family enzymes GH3, GH5, GH6, GH9, GH10, GH11, GH13 and GH43 that were associated with degrading Spartina biomass. While fungi were present, we did not detect a lignocellulolytic contribution from fungi which are major contributors to terrestrial lignocellulose deconstruction. Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. Conclusions Here, we identify a diverse cohort of previously undefined bacteria that drive lignocellulose degradation in the surface sediments of the salt marsh environment and describe the enzymatic mechanisms they employ to facilitate this process. Our results increase the understanding of the microbial and molecular mechanisms that underpin carbon sequestration from lignocellulose within salt marsh surface sediments in situ and provide insights into the potential enzymatic mechanisms regulating the enrichment of polyphenolics in salt marsh sediments.

‘You should tell somebody’: An evaluation of a survivor stories blog project to motivate sexual assault victims

2021 ◽  
pp. 001789692110164
Author(s):  
Rachel E Riggs
Keyword(s):  
Sexual Assault ◽  
Health Outcomes ◽  
Thematic Analysis ◽  
Social Cognitive ◽  
Cognitive Theory ◽  
Positive Health ◽  
Sexual Assault Survivors ◽  
Assault Victims ◽  
Positive Health Outcomes ◽  
Blog Posts

Objective: Sexual assault victims often do not disclose their assaults or seek positive health outcomes. The RAINN Survivor Stories project shares testimonials in the form of online blogs from sexual assault survivors to motivate and encourage others to come forward and disclose their assaults. This study aimed to better understand the themes present in the survivor stories to motivate victims to disclose their assaults and seek positive health outcomes. Design: A theoretical thematic analysis was conducted on blog posts created for the project to identify (a) how the posts tell survivor stories and (b) how the posts model positive health outcomes using social cognitive theory and the disclosure processes model as a guide. Setting: Online setting linked to the rainn.org website. Method: Blog posts were collected for inductive thematic analysis. Themes were identified based on their prevalence in the data and their pertinence to the research questions. Results: Emerging themes included (a) overcoming initial disclosure, (b) overcoming the lasting effects of victimisation, (c) utilising support and (d) advocating for others after assault. Conclusion: Findings offer insight to researchers and practitioners creating media messages for sexual assault victims and other stigmatised groups by expanding understanding of modelled positive health outcomes in media and the disclosure process of victims.

Understanding Variations in the Health Consequences of Sedentary Behavior: A Taxonomy of Social Interaction, Novelty, Choice, and Cognition

2021 ◽  
pp. 1-9
Author(s):  
Wendell C. Taylor
Keyword(s):  
Older Adults ◽  
Social Interaction ◽  
Health Outcomes ◽  
Sedentary Behavior ◽  
Age Groups ◽  
Sedentary Behaviors ◽  
Positive Health ◽  
Adverse Health Outcomes ◽  
Important Challenge ◽  
Positive Health Outcomes

The study of sedentary behaviors requires taxonomies (classification schemes) to standardize data collection, measurements, and outcomes. Three taxonomies of sedentary behaviors have been identified, but none address an important challenge in sedentary behavior research, which is to distinguish between beneficial and detrimental health effects of various sedentary behaviors. Some sedentary behaviors (e.g., reading) are associated with positive health outcomes, whereas other sedentary behaviors (e.g., television viewing) are associated with adverse health outcomes. To address directly this complexity and present a different conception and understanding of discrepant findings related to health outcomes, a new taxonomy is needed. The development of the new taxonomy is guided by analysis of literature and selection of a relevant and informative behavioral sciences theoretical framework (i.e., self-determination theory). Because older adults are an increasing percentage of the population and report a high prevalence of sedentary behaviors, the new taxonomy was designed for older adults with potential application to all age groups. Taylor’s taxonomy of sedentary behaviors is parsimonious with four domains: social interaction (i.e., not solitary, companionship, interacting, and connecting with others); novelty (i.e., refreshingly new, unusual, or different); choice (i.e., volition, preferred option or alternative, the power, freedom, or decision to choose); and cognition (i.e., mentally stimulating and engaging).

scholarly journals Implementation Research: An Efficient and Effective Tool to Accelerate Universal Health Coverage

2019 ◽  
Author(s):  
Agnes Binagwaho ◽  
Miriam F. Frisch ◽  
Kelechi Udoh ◽  
Laura Drown ◽  
Jovial Thomas Ntawukuriryayo ◽  
...  
Keyword(s):  
Health Outcomes ◽  
Universal Health Coverage ◽  
Implementation Research ◽  
Implementation Strategies ◽  
Health Coverage ◽  
Positive Health ◽  
Implementation Framework ◽  
Variable Success ◽  
Positive Health Outcomes ◽  
Universal Health

Success in the implementation of evidence-based interventions (EBIs) in different settings has had variable success. Implementation research offers the approach needed to understand the variability of health outcomes from implementation strategies in different settings and why interventions were successful in some countries and failed in others. When mastered and embedded into a policy and implementation framework, the application of implementation research by countries can provide policy-makers and implementers with the knowledge necessary to work towards universal health coverage (UHC) with the effectiveness, efficiency, sustainability, and fidelity needed to achieve sustainable positive health outcomes for all. To achieve this goal however, work is needed by the communities of research producers and consumers to create more clarity on implementation research methodologies and to build capacity to apply them as a critical tool for countries on their path to achieving UHC.

scholarly journals How involved are husbands in antenatal care? The perspective of women availing antenatal services at a maternity hospital in rural South Karnataka-India

2019 ◽  
Vol 8 (10) ◽  
pp. 3879
Author(s):  
Nancy A. Gnanaselvam ◽  
Apoorva Dore ◽  
Sadhana Beaty ◽  
Jacqueline V. Y. ◽  
Tresa Rose A. ◽  
...  
Keyword(s):  
Antenatal Care ◽  
Male Involvement ◽  
Maternity Hospital ◽  
Clinical Findings ◽  
Vital Role ◽  
Positive Health ◽  
Nuclear Families ◽  
Care Workers ◽  
Positive Health Outcomes ◽  
Antenatal Services

Background: Male involvement in maternal health is associated with significant positive health outcomes in the mother. We aimed to assess the antenatal womens’ perception of their husbands’ involvement in antenatal care in a maternity hospital in rural Karnataka, India.Methods: 200 pregnant women in a maternity hospital in Karnataka were interviewed using a semi-structured pilot tested questionnaire on their perception about their husbands’ participation in antenatal care.Results: The mean score of women’s perception of husband’s awareness of antenatal care was 10.1±2.2 out of a maximum of 12, while that of husband’s attitude to antenatal care was 7.7±1.4 out of a maximum of 9 and of husband’s participation was 21.7±5.4 out of a maximum of 30. The score of perception of husband’s awareness was significantly higher among multigravidae and among women who had a planned pregnancy. The score of perception of husband’s attitude was significantly higher among women from nuclear families, multigravidae, those with a living child and those with no complications in the current pregnancy. The score of perception of husband’s participation was significantly higher among women with no complications in the current pregnancy.Conclusions: Husbands play a vital role in the antenatal care of their wives. This should be harnessed by health care workers by providing health education to them, encouraging husbands to accompany their wives to antenatal checkups and labour and promptly communicating clinical findings to them.

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